FastQCFastQC Report
Wed 25 May 2016
SRR1033166_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033166_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4970458
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC59950.031.187744
GTATCAA65300.030.0799581
CAACGCA63300.029.6108255
ATCAACG63900.029.265753
GGTATCA44000.028.2621771
AACGCAG67750.027.7257586
GTACATG125400.025.3923851
TATCAAC80300.023.8263472
TACATGG127150.023.698472
ACATGGG124050.022.8779773
ACGCAGA80550.022.6788547
CGCAGAG81650.021.9132048
CATGGGG83050.019.6847384
GAGTACA93450.017.3897841
GCAGAGT99600.017.2567429
CTGTCGC12150.015.8469129
GTATTAG24400.015.6368591
AGTACAT96200.015.4415412
TATACTG32950.015.397485
AGAGTAC83850.015.37362910-11
Produced by FastQC (version 0.11.5)