##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033166_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4970458
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.70322996391882	33.0	31.0	34.0	30.0	34.0
2	31.861214399155973	34.0	31.0	34.0	30.0	34.0
3	31.97984732996436	34.0	31.0	34.0	30.0	34.0
4	35.375478275845005	37.0	35.0	37.0	33.0	37.0
5	35.46276721380605	37.0	35.0	37.0	35.0	37.0
6	35.605366547710496	37.0	37.0	37.0	35.0	37.0
7	35.56885864441466	37.0	37.0	37.0	35.0	37.0
8	35.58438578497193	37.0	37.0	37.0	35.0	37.0
9	37.287896407131896	39.0	39.0	39.0	35.0	39.0
10-11	37.27141241310157	39.0	38.0	39.0	35.0	39.0
12-13	37.23022073619775	39.0	38.0	39.0	35.0	39.0
14-15	38.71572267585804	41.0	39.0	41.0	36.0	41.0
16-17	38.723913973319966	41.0	39.0	41.0	36.0	41.0
18-19	38.71815534906442	41.0	39.0	41.0	35.0	41.0
20-21	38.740933833461625	41.0	39.0	41.0	35.0	41.0
22-23	38.70839166129157	41.0	39.0	41.0	35.0	41.0
24-25	38.7005746754122	41.0	39.0	41.0	35.0	41.0
26-27	38.655177148665175	41.0	39.0	41.0	35.0	41.0
28-29	38.626162518625044	41.0	39.0	41.0	34.5	41.0
30-31	38.62635998533737	41.0	39.0	41.0	35.0	41.0
32-33	38.68967145482368	41.0	39.0	41.0	35.0	41.0
34-35	38.66560677104605	41.0	39.0	41.0	35.0	41.0
36-37	38.595634949535835	41.0	39.0	41.0	35.0	41.0
38-39	38.52599589414094	41.0	39.0	41.0	34.0	41.0
40-41	38.47336845015087	41.0	39.0	41.0	34.0	41.0
42-43	38.37531571537271	41.0	39.0	41.0	34.0	41.0
44-45	38.270646789491025	40.5	38.0	41.0	34.0	41.0
46-47	38.15887710951385	40.0	38.0	41.0	33.0	41.0
48-49	38.05375621321013	40.0	38.0	41.0	33.0	41.0
50-51	37.945176681907384	40.0	38.0	41.0	33.0	41.0
52-53	37.810181073856775	40.0	38.0	41.0	33.0	41.0
54-55	37.67107548238009	40.0	37.0	41.0	33.0	41.0
56-57	37.527166912988704	40.0	37.0	41.0	32.5	41.0
58-59	37.34702767833467	40.0	37.0	41.0	32.0	41.0
60-61	37.13981025491011	40.0	36.0	41.0	32.0	41.0
62-63	36.89596864111919	39.0	36.0	41.0	31.0	41.0
64-65	36.63990229874189	39.0	35.0	41.0	31.0	41.0
66-67	36.35792747871524	39.0	35.0	41.0	31.0	41.0
68-69	36.06023177341001	38.0	35.0	40.0	31.0	41.0
70-71	35.715310239016205	37.0	35.0	40.0	30.5	41.0
72-73	35.352356161142495	37.0	35.0	39.5	30.0	41.0
74-75	34.978397564168134	36.0	35.0	39.0	30.0	41.0
76-77	34.63465177655661	36.0	34.0	39.0	30.0	40.5
78-79	34.25436207287135	35.5	34.0	37.5	29.0	39.5
80-81	33.87512076351918	35.0	34.0	37.0	29.0	39.0
82-83	33.633204324430466	35.0	34.0	37.0	29.0	39.0
84-85	33.34168521291197	35.0	34.0	36.0	29.0	38.0
86-87	33.07070837335311	35.0	34.0	36.0	29.0	37.0
88-89	32.6880346841277	35.0	34.0	36.0	28.5	37.0
90-91	32.48802977512334	35.0	34.0	35.0	28.0	36.5
92-93	32.30214257921503	35.0	34.0	35.0	27.5	36.0
94-95	32.12720165827777	35.0	34.0	35.0	27.0	36.0
96-97	31.949224296835425	35.0	34.0	35.0	27.0	36.0
98-99	31.761956544044835	35.0	34.0	35.0	26.5	36.0
100	31.609023353582305	35.0	34.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.07157350715557698
1101	2	0.135877594250168
1101	3	0.09489262419331368
1101	4	0.06320787391332061
1101	5	0.0721223796612449
1101	6	0.14095580921806317
1101	7	0.13796101696673446
1101	8	0.08882529239130577
1101	9	0.18313631325410284
1101	10-11	0.15879754419505332
1101	12-13	0.18618201548669333
1101	14-15	0.20778772996595052
1101	16-17	0.17125961939012058
1101	18-19	0.2020903307282822
1101	20-21	0.16735880694001537
1101	22-23	0.23612661782665612
1101	24-25	0.16178363374474003
1101	26-27	0.19197047769306153
1101	28-29	0.21100059704606622
1101	30-31	0.21449383530502075
1101	32-33	0.2100065786504146
1101	34-35	0.23257772971678037
1101	36-37	0.1970804427245625
1101	38-39	0.19325727715762753
1101	40-41	0.21730103280363977
1101	42-43	0.20885478216916908
1101	44-45	0.21327581575015842
1101	46-47	0.21205455212797375
1101	48-49	0.1230798765573482
1101	50-51	0.09171448533536619
1101	52-53	0.13874877467774382
1101	54-55	0.12687739525615171
1101	56-57	0.15953322683303384
1101	58-59	0.15710009412222803
1101	60-61	0.1307897059105798
1101	62-63	0.15753028469003993
1101	64-65	0.1016859971837718
1101	66-67	0.1106879604774278
1101	68-69	0.09223435087154996
1101	70-71	0.13319839418892343
1101	72-73	0.18294157264093514
1101	74-75	0.08896866543651782
1101	76-77	0.08161094900073351
1101	78-79	0.10371855843177968
1101	80-81	0.11640117038234621
1101	82-83	0.15298988563405658
1101	84-85	0.1250622457068431
1101	86-87	0.17735426952263111
1101	88-89	0.28501398400706535
1101	90-91	0.3290660752635901
1101	92-93	0.35732480243954257
1101	94-95	0.3749625906924763
1101	96-97	0.36162292544382524
1101	98-99	0.3809107837640866
1101	100	0.3895755673404082
1102	1	0.1425816835735425
1102	2	0.14559449180451622
1102	3	0.16408290704501383
1102	4	0.13020984601440944
1102	5	0.02774391633180784
1102	6	0.10622804704276234
1102	7	0.13792176414264645
1102	8	0.17798229240418806
1102	9	0.1705830002484987
1102	10-11	0.2419865307005793
1102	12-13	0.24734220085940706
1102	14-15	0.2640196115792506
1102	16-17	0.26508568812062805
1102	18-19	0.200899513564039
1102	20-21	0.16824161936611404
1102	22-23	0.20584854621624515
1102	24-25	0.17385563235971802
1102	26-27	0.19719503541779204
1102	28-29	0.23427394293102566
1102	30-31	0.23931652799632985
1102	32-33	0.21390984118239942
1102	34-35	0.20336677872593611
1102	36-37	0.20074930526228485
1102	38-39	0.12727185518235729
1102	40-41	0.15781558900225434
1102	42-43	0.15922512527008337
1102	44-45	0.12969212409043251
1102	46-47	0.14286978564295083
1102	48-49	0.12726323146795693
1102	50-51	0.12120221204634873
1102	52-53	0.14396168420670818
1102	54-55	0.2052905987026108
1102	56-57	0.13673135933775882
1102	58-59	0.11983223884570293
1102	60-61	0.10257434513165009
1102	62-63	0.11932571843773587
1102	64-65	0.12902491671217575
1102	66-67	0.11699664381985997
1102	68-69	0.14209829379037586
1102	70-71	0.13259507007650484
1102	72-73	0.15203686912911962
1102	74-75	0.11650911470811565
1102	76-77	0.10193318687672104
1102	78-79	0.1111859865090139
1102	80-81	0.1204620482756269
1102	82-83	0.10823045107987639
1102	84-85	0.12254462838062352
1102	86-87	0.08509916856457522
1102	88-89	0.2117743574833284
1102	90-91	0.28065089663873266
1102	92-93	0.3027398526151188
1102	94-95	0.26816763506788277
1102	96-97	0.2554372661964095
1102	98-99	0.3198861596833922
1102	100	0.33247164389299044
1103	1	0.07616105172017029
1103	2	0.06969572078061148
1103	3	0.08954643378591243
1103	4	0.12974867180454197
1103	5	0.042383886198571474
1103	6	0.13181273347208133
1103	7	0.1398445676165423
1103	8	0.16700152829516668
1103	9	0.19882641567909332
1103	10-11	0.2245082234673248
1103	12-13	0.2261053238156947
1103	14-15	0.25057360546510665
1103	16-17	0.2303405410188617
1103	18-19	0.18476372243476646
1103	20-21	0.15397294356850466
1103	22-23	0.22741322588832702
1103	24-25	0.17128110834359234
1103	26-27	0.1915148666119748
1103	28-29	0.25877794084483696
1103	30-31	0.24404455110286705
1103	32-33	0.23703953995079274
1103	34-35	0.2391965858670062
1103	36-37	0.2801387418597514
1103	38-39	0.26274669490641855
1103	40-41	0.21904422394047884
1103	42-43	0.20891050462829952
1103	44-45	0.21336665038096925
1103	46-47	0.22018532724420936
1103	48-49	0.26209352326514335
1103	50-51	0.2584035903517403
1103	52-53	0.22198111871728088
1103	54-55	0.21619709748710392
1103	56-57	0.16735847032201434
1103	58-59	0.18594671613067248
1103	60-61	0.22522534072972888
1103	62-63	0.24572023329027814
1103	64-65	0.2673300747970515
1103	66-67	0.2654407440366313
1103	68-69	0.1735815502801472
1103	70-71	0.15798658419325307
1103	72-73	0.23957566635899497
1103	74-75	0.2466941234775888
1103	76-77	0.20630216253088918
1103	78-79	0.1883200207924247
1103	80-81	0.2292667493965439
1103	82-83	0.2079831597025077
1103	84-85	0.20243805785695201
1103	86-87	0.22106261422562312
1103	88-89	0.29825121699850854
1103	90-91	0.32468959386434193
1103	92-93	0.2932753859035131
1103	94-95	0.26236700755843856
1103	96-97	0.2781993928427866
1103	98-99	0.34523729664167035
1103	100	0.3888664352330409
1104	1	-0.025225700775639126
1104	2	-0.051233642523982326
1104	3	-0.04621883298674945
1104	4	-0.053303411663442546
1104	5	-0.07804647672254816
1104	6	-0.030244710008652476
1104	7	0.011720496678236714
1104	8	-0.005401084495616715
1104	9	-0.0034357014397699004
1104	10-11	0.020881058888214454
1104	12-13	0.06547499988824512
1104	14-15	0.0022904845502864646
1104	16-17	0.061229768858176215
1104	18-19	0.02117117280940306
1104	20-21	-0.013436299221979198
1104	22-23	-0.032155840288865534
1104	24-25	-0.021883744363776714
1104	26-27	0.01919277250152618
1104	28-29	0.06440638510544261
1104	30-31	0.07299325181681837
1104	32-33	0.09023405800205353
1104	34-35	0.058642197248843786
1104	36-37	0.11734101124779528
1104	38-39	0.05310567357408047
1104	40-41	0.03866864603717346
1104	42-43	0.05988402663925285
1104	44-45	0.0467227389935303
1104	46-47	0.048388085668783276
1104	48-49	-0.03330945253294004
1104	50-51	0.04012754646340255
1104	52-53	-0.026177454071607542
1104	54-55	-0.02292830268265078
1104	56-57	-0.014987795550126748
1104	58-59	-0.028651185967227377
1104	60-61	-0.020358923281058594
1104	62-63	-0.012294068335393149
1104	64-65	-0.0872115827136426
1104	66-67	-0.025017873131780277
1104	68-69	-0.04586672710482276
1104	70-71	-0.010053208731008567
1104	72-73	-0.015433591942773717
1104	74-75	-0.06647049225173163
1104	76-77	-0.11951492703388311
1104	78-79	-0.14996898148687166
1104	80-81	-0.07817686598376383
1104	82-83	-0.04346846553609396
1104	84-85	0.011977717211763661
1104	86-87	0.07437535201155043
1104	88-89	0.13028076708489778
1104	90-91	0.04371265721503903
1104	92-93	0.014253114274957568
1104	94-95	0.06002639145858524
1104	96-97	0.0729456970622877
1104	98-99	0.08901971954840349
1104	100	0.11928746983614147
1105	1	0.05673205043521179
1105	2	0.11457381129582345
1105	3	0.06376415973493721
1105	4	0.05660185094303927
1105	5	0.10550876916418161
1105	6	0.12461051740559981
1105	7	0.1223656822359942
1105	8	0.1440363125321653
1105	9	0.17318563217819616
1105	10-11	0.15885757679175327
1105	12-13	0.14867096800530533
1105	14-15	0.161340568188443
1105	16-17	0.17413222947813978
1105	18-19	0.17163080848813905
1105	20-21	0.18321366475866796
1105	22-23	0.2278410067870169
1105	24-25	0.19436941200668656
1105	26-27	0.22285934161988052
1105	28-29	0.26632076736272836
1105	30-31	0.29650291914295224
1105	32-33	0.28451619764212666
1105	34-35	0.2504095303555758
1105	36-37	0.25440320085413504
1105	38-39	0.2576000871282247
1105	40-41	0.2192142870260554
1105	42-43	0.2261753784894296
1105	44-45	0.2339723316341562
1105	46-47	0.2765884808772796
1105	48-49	0.25886317212966503
1105	50-51	0.30972781437333907
1105	52-53	0.3129586545701599
1105	54-55	0.25317463979705934
1105	56-57	0.248324722444778
1105	58-59	0.23066588200634897
1105	60-61	0.21276848202979437
1105	62-63	0.2801620437881809
1105	64-65	0.28721692271367516
1105	66-67	0.27321198941175595
1105	68-69	0.21458067696141114
1105	70-71	0.19615489095574645
1105	72-73	0.2232892000552198
1105	74-75	0.21859271196203878
1105	76-77	0.2205620370054504
1105	78-79	0.20897647075982206
1105	80-81	0.1671948935054388
1105	82-83	0.1759862381107098
1105	84-85	0.15245920476175456
1105	86-87	0.20060871447809348
1105	88-89	0.23010333879176414
1105	90-91	0.23383583197526292
1105	92-93	0.21999893935837633
1105	94-95	0.24924442492408616
1105	96-97	0.271281126436989
1105	98-99	0.27825435460678705
1105	100	0.31346133479330973
1106	1	0.07782124338036311
1106	2	0.08970219578708694
1106	3	0.05009421933369396
1106	4	0.06225449931037019
1106	5	0.018443844758536443
1106	6	0.043092194751544355
1106	7	0.047143842415820814
1106	8	0.074354545648184
1106	9	0.07413268348535951
1106	10-11	0.05229765625676208
1106	12-13	0.021716240676617815
1106	14-15	0.06131272745422933
1106	16-17	0.019646420324740177
1106	18-19	0.06842436734540769
1106	20-21	0.054649257994810796
1106	22-23	0.08423658771168618
1106	24-25	0.10470573926821913
1106	26-27	0.10871960631671129
1106	28-29	0.12321202527891728
1106	30-31	0.08226133431964655
1106	32-33	0.026573986985233944
1106	34-35	0.02854196271238152
1106	36-37	0.0821505263715423
1106	38-39	0.04479927195899336
1106	40-41	0.03209848449474606
1106	42-43	0.04342455539234891
1106	44-45	0.0083617941261096
1106	46-47	0.058090015148899
1106	48-49	0.06178887421048529
1106	50-51	0.018550249248399098
1106	52-53	-0.00832579658963084
1106	54-55	0.0033001795901910214
1106	56-57	0.030530575994120568
1106	58-59	0.013275775959733949
1106	60-61	0.011187202941592034
1106	62-63	0.050279609099646905
1106	64-65	0.017865234082208303
1106	66-67	0.06237159096748002
1106	68-69	0.0016192995678991906
1106	70-71	0.08521851892518839
1106	72-73	0.11246125299458498
1106	74-75	0.09312963241308836
1106	76-77	0.05644137517010961
1106	78-79	0.06569084441324691
1106	80-81	0.08896728034707735
1106	82-83	0.04210094007028431
1106	84-85	0.08762065303955069
1106	86-87	0.09054720371020863
1106	88-89	0.17251727501457026
1106	90-91	0.14811584354059448
1106	92-93	0.19555458818271632
1106	94-95	0.1806093670508062
1106	96-97	0.1588455984889947
1106	98-99	0.2272688299232044
1106	100	0.22005459892120172
1107	1	0.18603282808215127
1107	2	0.1825748788556396
1107	3	0.16211677415667225
1107	4	0.14339395100037677
1107	5	0.08722475425940246
1107	6	0.06166980070342021
1107	7	0.05793008850116621
1107	8	0.08901166920419712
1107	9	0.12972458271410403
1107	10-11	0.11258571417848628
1107	12-13	0.05378649322860696
1107	14-15	0.05872306695297169
1107	16-17	0.044848315756915724
1107	18-19	0.12652471973110124
1107	20-21	0.11204393887811648
1107	22-23	0.11040869345234938
1107	24-25	0.11588748793541015
1107	26-27	0.18154679295279408
1107	28-29	0.1419520280951474
1107	30-31	0.1635848660905097
1107	32-33	0.16864786677484034
1107	34-35	0.15941274036717346
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2216	10-11	-0.2418451540312887
2216	12-13	-0.2555971964591137
2216	14-15	-0.29407416029115296
2216	16-17	-0.2542955812324479
2216	18-19	-0.2824053204939361
2216	20-21	-0.24919652024982497
2216	22-23	-0.2606342502569987
2216	24-25	-0.2552775512942702
2216	26-27	-0.3543436280453349
2216	28-29	-0.26438415740502563
2216	30-31	-0.3329827254088329
2216	32-33	-0.2600529079698717
2216	34-35	-0.2896469470078955
2216	36-37	-0.28063768187740123
2216	38-39	-0.27281029600379725
2216	40-41	-0.2753738104896186
2216	42-43	-0.30188198374481345
2216	44-45	-0.2775547207373066
2216	46-47	-0.1991281368257276
2216	48-49	-0.20250683998869334
2216	50-51	-0.2052314244433191
2216	52-53	-0.19394464412598467
2216	54-55	-0.17749145032605185
2216	56-57	-0.24423482704904131
2216	58-59	-0.2568256552013679
2216	60-61	-0.18323957175284278
2216	62-63	-0.11660586846375054
2216	64-65	-0.15271511969691431
2216	66-67	-0.1834292210136752
2216	68-69	-0.19471371760101164
2216	70-71	-0.2024725869559063
2216	72-73	-0.09321765100813906
2216	74-75	-0.11364256521035543
2216	76-77	-0.18085217610983761
2216	78-79	-0.2052033099429451
2216	80-81	-0.12690403334565303
2216	82-83	-0.17849500031942966
2216	84-85	-0.1653953117864262
2216	86-87	-0.18371123558991798
2216	88-89	-0.142891389663518
2216	90-91	-0.14311589759461896
2216	92-93	-0.126079885854125
2216	94-95	-0.1397380223027298
2216	96-97	-0.146160399770622
2216	98-99	-0.19237194671844904
2216	100	-0.17157079646900186
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	37547.0
3	6465.0
4	4172.0
5	3650.0
6	3288.0
7	3722.0
8	4576.0
9	4931.0
10	5470.0
11	6045.0
12	6528.0
13	7507.0
14	8171.0
15	8844.0
16	8803.0
17	8888.0
18	8942.0
19	8917.0
20	9108.0
21	9486.0
22	10961.0
23	14569.0
24	22041.0
25	31307.0
26	34143.0
27	29973.0
28	30901.0
29	39441.0
30	48280.0
31	59502.0
32	78856.0
33	109237.0
34	151592.0
35	229562.0
36	431048.0
37	948450.0
38	1694647.0
39	838762.0
40	2126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.36517828899651	20.281550483741416	13.53351569901225	23.819755528249825
2	14.917367224373447	20.389895210425138	41.19843307115797	23.494304494043448
3	17.72498750323869	25.935928405183756	30.50973297898833	25.829351112589226
4	11.827486718948906	16.989400317396743	40.08024482016365	31.102868143490696
5	12.399899605244116	38.476954391837836	36.56790423129277	12.555241771625278
6	30.208592388701767	39.07271256624775	18.158463414339696	12.560231630710788
7	26.505465576495634	32.91812429792759	23.43846923215134	17.137940893425434
8	24.359773556932517	37.313646667844466	20.955400760524824	17.371179014698196
9	24.674013327014922	16.994087055208613	21.59064955866714	36.741250059109326
10-11	23.299439866858332	27.26806331533903	29.79957413742605	19.632922680376584
12-13	23.90301701760839	24.65685891796568	29.6311872242778	21.808936840148128
14-15	21.24931860123548	26.734203873705443	26.455376203552955	25.561101321506126
16-17	21.046710737405625	30.075829400098865	27.12568974348681	21.751770119008704
18-19	20.948313687430783	29.231658187153243	28.885538692670217	20.934489432745757
20-21	22.149367048105713	28.5028732946149	28.307171509522828	21.04058814775656
22-23	21.671154690693566	28.285537931558345	28.41621966685162	21.62708771089647
24-25	21.645644740379648	28.333215274077244	28.35053174962208	21.670608235921033
26-27	21.360010914488882	28.371010521897293	28.411024277694864	21.85795428591896
28-29	21.388986846414394	28.4249855243651	28.47273625468666	21.71329137453385
30-31	21.63902942387386	28.808872599306735	28.08963554094555	21.462462435873856
32-33	21.33418107894579	28.461321589661875	28.542369907137044	21.662127424255292
34-35	21.456623496832062	28.30005759890537	28.60114141411601	21.642177490146565
36-37	21.803728306242	28.5418768453301	28.114530889271546	21.539863959156357
38-39	21.55005421768082	28.66496531738521	28.295462113924135	21.489518351009835
40-41	21.35590932019523	28.45187627855374	28.52972381528987	21.662490585961166
42-43	21.36578213706815	28.30141262899334	28.503806017838144	21.828999216100364
44-45	21.46091544740279	28.488430700399412	28.46085528652173	21.589798565676062
46-47	21.51825230503516	28.632666897181082	28.33339386264929	21.51568693513447
48-49	21.466477795669846	28.52631429637489	28.314342449165636	21.69286545878963
50-51	21.515298209863577	28.41045816965532	28.32448445954266	21.74975916093844
52-53	21.497945104326245	28.406583633831396	28.407277787368496	21.688193474473863
54-55	21.607772833170248	28.735985085766202	28.217493743568657	21.438748337494896
56-57	21.448004686228227	28.46735771029102	28.26256156936737	21.822076034113376
58-59	21.361184072204825	28.124109083848982	28.62522521253726	21.889481631408938
60-61	21.584566724547454	28.50637897491959	28.241383497306536	21.66767080322642
62-63	21.38981410597539	28.544264386189578	28.22858959359637	21.837331914238664
64-65	21.4262921915726	28.28393860775984	28.35774651004409	21.93202269062347
66-67	21.268827992990847	28.348035659802008	28.268665127475213	22.11447121973194
68-69	21.700850500698397	28.48633610697125	28.15834835168649	21.654465040643863
70-71	21.60248401842653	28.309891076725535	28.20579343418304	21.881831470664896
72-73	21.433232639582936	28.348015225884932	28.410344821689172	21.808407312842963
74-75	21.45194365573766	28.440493226063623	28.269096414478895	21.838466703719817
76-77	21.530300984799357	28.361052590704173	28.155479471761087	21.953166952735383
78-79	21.640915540168056	28.439965410315903	28.25820458776066	21.660914461755382
80-81	21.591532685728758	28.287181036908393	28.30649590047051	21.81479037689234
82-83	21.38986887175386	28.283693722241505	28.524492865154695	21.801944540849945
84-85	21.406273231779295	28.24873704628681	28.3388323384557	22.006157383478197
86-87	21.596737198207887	28.369940061317866	28.25246294033424	21.78085980014001
88-89	21.40707137657255	28.238110896315415	28.31801408542931	22.036803641682727
90-91	21.377317246871637	28.10740948126303	28.488679514944515	22.026593756920818
92-93	21.566455307038936	28.289491063816698	28.22768945063872	21.916364178505653
94-95	21.355657861353638	28.073529826988818	28.44932164077306	22.121490670884477
96-97	21.366330066711782	28.045725617755185	28.445504476412047	22.14243983912099
98-99	21.431062308308515	28.15240274914479	28.3379263054653	22.078608637081395
100	21.53894137491484	27.99283352891625	28.260179655625645	22.208045440543266
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	324.0
1	304.5
2	300.5
3	313.0
4	268.5
5	214.5
6	191.0
7	193.5
8	208.5
9	208.0
10	213.0
11	233.5
12	259.0
13	327.5
14	402.5
15	454.0
16	601.0
17	828.0
18	1198.5
19	1787.0
20	2722.5
21	4280.5
22	6779.5
23	10893.5
24	16251.0
25	22441.5
26	31535.5
27	44112.5
28	59418.0
29	75256.0
30	90223.0
31	106629.0
32	123577.0
33	139321.0
34	153749.5
35	165698.0
36	174798.0
37	182718.0
38	187418.5
39	189148.0
40	191469.5
41	191619.0
42	186315.5
43	179414.0
44	174173.0
45	168868.5
46	164514.0
47	159287.0
48	153146.0
49	148939.5
50	144393.5
51	138624.5
52	129137.5
53	120297.0
54	115020.0
55	108833.0
56	100540.0
57	91109.0
58	82814.0
59	75373.5
60	67093.0
61	57049.5
62	48200.0
63	40523.5
64	33260.5
65	26854.5
66	20609.0
67	15184.5
68	11180.0
69	8469.5
70	6170.0
71	4463.0
72	3557.0
73	2684.5
74	1901.0
75	1334.5
76	808.0
77	503.0
78	341.0
79	209.0
80	136.5
81	91.0
82	63.5
83	47.0
84	35.5
85	28.0
86	19.5
87	16.0
88	15.0
89	12.5
90	15.0
91	12.0
92	6.5
93	5.5
94	4.0
95	2.0
96	4.0
97	5.5
98	3.0
99	4.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33548216281075105
2	0.11753444048818038
3	0.06379693782745977
4	0.038487398947944035
5	0.041666180460633605
6	0.009616820019402639
7	0.007061723487050891
8	0.014727013084106134
9	0.017161396394457008
10-11	0.02041059395331376
12-13	0.01573295660078005
14-15	0.018127102170463972
16-17	0.01906262964097071
18-19	0.018740727715635057
20-21	0.023388186762668548
22-23	0.015099212185275481
24-25	0.024182882140840946
26-27	0.018449004095799623
28-29	0.01811704273529723
30-31	0.019233640038805278
32-33	0.02414264440017399
34-35	0.014998617833608091
36-37	0.009657057760069596
38-39	0.013620475215764824
40-41	0.009797889852403943
42-43	0.008731589724729592
44-45	0.009073610520398723
46-47	0.008339271753226766
48-49	0.004476448649198927
50-51	0.00671970269138176
52-53	0.007564695245387849
54-55	0.003470505132525011
56-57	0.0030077711148550094
58-59	0.003078187161022183
60-61	0.0037119315765267503
62-63	0.003078187161022183
64-65	0.002474621051017834
66-67	0.002001827598181093
68-69	0.003027889985188488
70-71	0.0025047993565180515
72-73	0.0021426596905154416
74-75	0.0024243238751841378
76-77	0.004164606159030013
78-79	0.0038225853633608816
80-81	0.003872882539194577
82-83	0.002675809754352617
84-85	0.0033196136050239237
86-87	0.0025852748378519647
88-89	0.6056685319541981
90-91	0.6166836134617776
92-93	0.612770493181916
94-95	0.6000151293904907
96-97	0.5980635989681434
98-99	0.5950357089829549
100	0.5969671205349688
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4970458.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.099451515607264
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.62244449400909	36.43814164292087
2	16.36468659203437	17.379117645236747
3	6.358870008969837	10.12957529206029
4	3.1339292964647	6.656397069238741
5	1.6797954233073464	4.459810781802371
6	0.9813718417436678	3.126618391767006
7	0.6644262875204999	2.46964700079228
8	0.46581369617711915	1.9787561420370199
9	0.32152883952500366	1.536570452270466
>10	1.3307073373145486	11.927920694179589
>50	0.05460781727466364	1.9657050321388356
>100	0.02143951816012781	1.8126094753950401
>500	3.7884749897213035E-4	0.11913038016075195
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	2.0118870333478324E-5	0.0
20-21	0.0	0.0	0.0	3.0178305500217485E-5	0.0
22-23	0.0	0.0	0.0	1.0059435166739161E-4	0.0
24-25	0.0	0.0	0.0	1.1065378683413078E-4	0.0
26-27	0.0	0.0	0.0	2.5148587916847903E-4	0.0
28-29	0.0	0.0	0.0	9.757652111736987E-4	0.0
30-31	0.0	0.0	0.0	0.004647459047033492	0.0
32-33	0.0	0.0	0.0	0.012654769439757865	0.0
34-35	0.0	0.0	0.0	0.023539078290169636	0.0
36-37	0.0	0.0	0.0	0.044593476094154705	0.0
38-39	0.0	0.0	0.0	0.07944941894690591	0.0
40-41	0.0	0.0	0.0	0.12108542110203929	0.0
42-43	0.0	0.0	0.0	0.16441140836518486	0.0
44-45	0.0	0.0	0.0	0.2142257313108772	0.0
46-47	0.0	2.0118870333478324E-5	0.0	0.2764131595116587	0.0
48-49	0.0	2.0118870333478324E-5	0.0	0.34657771979966434	0.0
50-51	0.0	2.0118870333478324E-5	0.0	0.4228483572338807	0.0
52-53	0.0	2.0118870333478324E-5	0.0	0.49872667669659415	0.0
54-55	0.0	2.0118870333478324E-5	0.0	0.5812844611100225	0.0
56-57	0.0	4.023774066695665E-5	0.0	0.6776337311370502	0.0
58-59	0.0	4.023774066695665E-5	0.0	0.7912852296508692	0.0
60-61	0.0	4.023774066695665E-5	0.0	0.9094131768138871	0.0
62-63	0.0	4.023774066695665E-5	0.0	1.0297944374542547	0.0
64-65	0.0	4.023774066695665E-5	0.0	1.1590481199116862	0.0
66-67	0.0	4.023774066695665E-5	0.0	1.3048898109590705	0.0
68-69	0.0	4.023774066695665E-5	0.0	1.4707598374234325	0.0
70-71	0.0	4.023774066695665E-5	0.0	1.6542137565592547	0.0
72-73	0.0	4.023774066695665E-5	0.0	1.8447595774876278	0.0
74-75	0.0	8.04754813339133E-5	0.0	2.040405532045538	0.0
76-77	0.0	1.0059435166739161E-4	0.0	2.251311649751391	2.0118870333478324E-5
78-79	0.0	1.0059435166739161E-4	0.0	2.4902131755262795	2.0118870333478324E-5
80-81	0.0	1.0059435166739161E-4	0.0	2.749273809375313	2.0118870333478324E-5
82-83	0.0	1.0059435166739161E-4	0.0	3.0219146806994446	2.0118870333478324E-5
84-85	0.0	1.0059435166739161E-4	0.0	3.2943342444499075	2.0118870333478324E-5
86-87	0.0	1.0059435166739161E-4	0.0	3.5841968687794967	2.0118870333478324E-5
88	0.0	1.0059435166739161E-4	0.0	3.820513119716533	2.0118870333478324E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	5995	0.0	31.18774	4
GTATCAA	6530	0.0	30.079958	1
CAACGCA	6330	0.0	29.610825	5
ATCAACG	6390	0.0	29.26575	3
GGTATCA	4400	0.0	28.262177	1
AACGCAG	6775	0.0	27.725758	6
GTACATG	12540	0.0	25.392385	1
TATCAAC	8030	0.0	23.826347	2
TACATGG	12715	0.0	23.69847	2
ACATGGG	12405	0.0	22.877977	3
ACGCAGA	8055	0.0	22.678854	7
CGCAGAG	8165	0.0	21.913204	8
CATGGGG	8305	0.0	19.684738	4
GAGTACA	9345	0.0	17.389784	1
GCAGAGT	9960	0.0	17.256742	9
CTGTCGC	1215	0.0	15.846912	9
GTATTAG	2440	0.0	15.636859	1
AGTACAT	9620	0.0	15.441541	2
TATACTG	3295	0.0	15.39748	5
AGAGTAC	8385	0.0	15.373629	10-11
>>END_MODULE