FastQCFastQC Report
Wed 25 May 2016
SRR1033166_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033166_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4970458
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66210.13320704047795998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56020.11270591160814558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62950.049.0382421
GTATCAA116650.041.1070481
TCAACGC132400.035.512424
ATCAACG134150.035.0842063
CAACGCA136800.034.335845
AACGCAG143000.032.9627466
TATCAAC150600.031.533272
ACGCAGA165250.028.1547857
CGCAGAG165900.027.8736658
GTACATG129100.025.4058281
GCAGAGT186450.024.5492129
TACATGG130850.023.6801412
ACATGGG131350.022.6948553
GAGTACT122200.020.72805612-13
AGAGTAC170850.020.69819610-11
CAGAGTA185750.019.74627110-11
CATGGGG83200.019.1012424
AGTACTT132500.018.63793812-13
GTATAGA21100.018.5106831
GTACTTT138850.018.24248114-15
Produced by FastQC (version 0.11.5)