FastQCFastQC Report
Wed 25 May 2016
SRR1033165_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033165_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4379976
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54750.038.2089541
TCAACGC59700.034.2286344
ATCAACG63200.032.340453
CAACGCA64600.031.7057865
GGTATCA41400.031.1829031
AACGCAG65250.031.0923736
TATCAAC71450.028.7538682
GTACATG165450.028.6766871
TACATGG168150.026.9803452
ACGCAGA77250.026.0801097
ACATGGG175000.024.8894633
CGCAGAG85250.023.0820948
CATGGGG139800.021.438194
GAGTACA108200.020.6403681
AGTACAT113300.018.8798482
GCAGAGT102800.018.6399
GTATAGA15550.017.8767241
ATGGGGA65750.016.5758865
TAGACTG14050.016.0490555
GTCTAGG13850.015.9887451
Produced by FastQC (version 0.11.5)