##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033165_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4379976 Sequences flagged as poor quality 0 Sequence length 100 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7943205624871 33.0 31.0 34.0 30.0 34.0 2 31.838223086153896 34.0 31.0 34.0 30.0 34.0 3 32.03194058597581 34.0 31.0 34.0 30.0 34.0 4 35.452577594032476 37.0 35.0 37.0 35.0 37.0 5 35.50609090095471 37.0 35.0 37.0 35.0 37.0 6 35.58799203465955 37.0 37.0 37.0 35.0 37.0 7 35.51556492546991 37.0 36.0 37.0 35.0 37.0 8 35.518723161953396 37.0 36.0 37.0 35.0 37.0 9 37.24598673600038 39.0 38.0 39.0 35.0 39.0 10-11 37.24222552817641 39.0 38.0 39.0 35.0 39.0 12-13 37.2131732000358 39.0 38.0 39.0 35.0 39.0 14-15 38.67957826709553 41.0 39.0 41.0 35.5 41.0 16-17 38.65969665130585 41.0 39.0 41.0 35.0 41.0 18-19 38.617880212129016 41.0 39.0 41.0 35.0 41.0 20-21 38.628309150552425 41.0 39.0 41.0 35.0 41.0 22-23 38.56754820117736 41.0 39.0 41.0 35.0 41.0 24-25 38.52987562032303 41.0 39.0 41.0 34.0 41.0 26-27 38.467986468419 40.5 39.0 41.0 34.0 41.0 28-29 38.42780222996656 40.0 39.0 41.0 34.0 41.0 30-31 38.42061372025783 40.0 39.0 41.0 34.0 41.0 32-33 38.494436270883675 41.0 39.0 41.0 34.0 41.0 34-35 38.465725382970135 41.0 39.0 41.0 34.0 41.0 36-37 38.38827358871373 41.0 39.0 41.0 34.0 41.0 38-39 38.30736081658895 41.0 39.0 41.0 34.0 41.0 40-41 38.23589181767206 41.0 38.0 41.0 34.0 41.0 42-43 38.110451175988175 40.0 38.0 41.0 33.0 41.0 44-45 37.96152090787712 40.0 38.0 41.0 33.0 41.0 46-47 37.818855628432665 40.0 38.0 41.0 33.0 41.0 48-49 37.67891330911402 40.0 37.5 41.0 33.0 41.0 50-51 37.52270800113973 40.0 37.0 41.0 32.5 41.0 52-53 37.350896443268184 40.0 37.0 41.0 32.0 41.0 54-55 37.16515467208039 40.0 36.0 41.0 32.0 41.0 56-57 36.97439380946379 40.0 36.0 41.0 31.5 41.0 58-59 36.74208991099495 39.0 35.0 41.0 31.0 41.0 60-61 36.48663177606453 39.0 35.0 41.0 31.0 41.0 62-63 36.19233233241461 39.0 35.0 41.0 31.0 41.0 64-65 35.893263570394 38.0 35.0 40.0 30.5 41.0 66-67 35.57033052235903 37.0 35.0 40.0 30.0 41.0 68-69 35.237631553232255 37.0 35.0 39.5 30.0 41.0 70-71 34.87635582934701 36.0 34.5 39.0 30.0 41.0 72-73 34.50139144598052 36.0 34.0 39.0 29.0 41.0 74-75 34.125025913384 35.0 34.0 37.5 29.0 40.0 76-77 33.8067596717425 35.0 34.0 37.0 29.0 39.0 78-79 33.461881526291464 35.0 34.0 37.0 29.0 39.0 80-81 33.13598487754271 35.0 34.0 36.0 28.0 39.0 82-83 32.96234088953912 35.0 34.0 36.0 29.0 37.0 84-85 32.736153120473716 35.0 34.0 35.5 28.5 37.0 86-87 32.52861944905634 35.0 34.0 35.0 28.0 36.5 88-89 32.212555045963725 35.0 34.0 35.0 27.0 36.0 90-91 32.05947361355405 35.0 33.5 35.0 27.0 36.0 92-93 31.90837997742453 35.0 33.0 35.0 26.5 36.0 94-95 31.759816834612792 35.0 33.0 35.0 26.0 35.5 96-97 31.5992407264332 35.0 33.0 35.0 25.5 35.0 98-99 31.418127405264322 35.0 33.0 35.0 25.0 35.0 100 31.26560282522096 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12928169367512865 1101 2 0.12457631288856774 1101 3 0.12864058615862461 1101 4 0.07480383752854891 1101 5 0.0950266885991411 1101 6 0.07701634277587033 1101 7 0.07363906119834951 1101 8 0.027259615551024297 1101 9 0.14740983696578525 1101 10-11 0.1183147700091709 1101 12-13 0.16552927516809746 1101 14-15 0.21573809223838936 1101 16-17 0.1433790122919305 1101 18-19 0.17694284249750325 1101 20-21 0.1551981308532291 1101 22-23 0.12474596153550266 1101 24-25 0.11554585180731891 1101 26-27 0.1354296767912757 1101 28-29 0.2363377752789546 1101 30-31 0.19178506626095526 1101 32-33 0.22368027102350396 1101 34-35 0.19031204919775746 1101 36-37 0.23073977567894133 1101 38-39 0.16565447412376955 1101 40-41 0.18720297531482544 1101 42-43 0.1628955054961878 1101 44-45 0.14528466856936006 1101 46-47 0.10327506741384695 1101 48-49 0.1325725602102068 1101 50-51 0.09595469210206886 1101 52-53 0.12294939238729796 1101 54-55 0.09874133607244318 1101 56-57 0.13513949281328763 1101 58-59 0.1673143736838938 1101 60-61 0.1014589021014416 1101 62-63 0.13004664891609963 1101 64-65 0.14931791603648037 1101 66-67 0.10644052741957921 1101 68-69 0.11301034656130327 1101 70-71 0.12903993125640056 1101 72-73 0.1969739738720122 1101 74-75 0.13645318702317155 1101 76-77 0.10866631467566634 1101 78-79 0.1345787646324581 1101 80-81 0.18220088853188798 1101 82-83 0.12566862320426964 1101 84-85 0.04965462263997722 1101 86-87 0.1811605122736708 1101 88-89 0.30561162959792654 1101 90-91 0.37247827750522333 1101 92-93 0.35826061665979836 1101 94-95 0.3595214858304594 1101 96-97 0.3352576890053669 1101 98-99 0.41539263001212134 1101 100 0.3575370247163434 1102 1 0.17219047036552126 1102 2 0.15877884139307596 1102 3 0.18788225852934914 1102 4 0.15449559631633036 1102 5 0.05344180787091801 1102 6 0.1183854196573364 1102 7 0.039673352080725977 1102 8 0.12338419766642517 1102 9 0.14169308397250546 1102 10-11 0.1408245316444905 1102 12-13 0.17838354518943333 1102 14-15 0.21021529689799223 1102 16-17 0.23160201436267158 1102 18-19 0.2746898021593793 1102 20-21 0.19188747058344546 1102 22-23 0.21370060343775776 1102 24-25 0.22329969731197252 1102 26-27 0.23557938551838475 1102 28-29 0.23096165954828507 1102 30-31 0.2402967825353315 1102 32-33 0.21853262660943784 1102 34-35 0.25731137700504547 1102 36-37 0.2450681653948834 1102 38-39 0.20008653995053294 1102 40-41 0.22087420383041234 1102 42-43 0.22938960199838476 1102 44-45 0.2069293392014373 1102 46-47 0.22326996664599363 1102 48-49 0.19596384650274956 1102 50-51 0.14441827199106427 1102 52-53 0.12419065214460545 1102 54-55 0.17345360245556662 1102 56-57 0.10553071495167643 1102 58-59 0.119841514534059 1102 60-61 0.0945628561213212 1102 62-63 0.12698304556391093 1102 64-65 0.21222447432756297 1102 66-67 0.13923977715094793 1102 68-69 0.12909440606369316 1102 70-71 0.1809245448103809 1102 72-73 0.23704770159218214 1102 74-75 0.21834122203998874 1102 76-77 0.14156273705269484 1102 78-79 0.1799612143749343 1102 80-81 0.17042968025933192 1102 82-83 0.18887133724914662 1102 84-85 0.19937169781616149 1102 86-87 0.16882980331705966 1102 88-89 0.29866156343496897 1102 90-91 0.3568325124591354 1102 92-93 0.3333519546550008 1102 94-95 0.32637452443528403 1102 96-97 0.3413335059304963 1102 98-99 0.4233513313469892 1102 100 0.3626571744909697 1103 1 0.12261964792996238 1103 2 0.09070571324633292 1103 3 0.14814706109724085 1103 4 0.175371611261113 1103 5 0.15885390002618038 1103 6 0.23435380452346521 1103 7 0.16273627174334848 1103 8 0.20174784903812792 1103 9 0.22322339179920192 1103 10-11 0.24582753185595863 1103 12-13 0.2553112462094518 1103 14-15 0.25335026418445494 1103 16-17 0.2330622972218208 1103 18-19 0.2366938205599709 1103 20-21 0.2534387155355873 1103 22-23 0.2364156240887425 1103 24-25 0.245952636827802 1103 26-27 0.2689650749617627 1103 28-29 0.3088666630989678 1103 30-31 0.29712625787647085 1103 32-33 0.27167986226638874 1103 34-35 0.26962175098212526 1103 36-37 0.2583701488851631 1103 38-39 0.2704196527755016 1103 40-41 0.28501276552020016 1103 42-43 0.1822318595913046 1103 44-45 0.1290799148208066 1103 46-47 0.1860612413864331 1103 48-49 0.29161410896408313 1103 50-51 0.2282416593654304 1103 52-53 0.10647953852313208 1103 54-55 0.1785169047368953 1103 56-57 0.1282399649424164 1103 58-59 0.16407010171474212 1103 60-61 0.13173731642364572 1103 62-63 0.16221464157216303 1103 64-65 0.23587766605048444 1103 66-67 0.19780971819128723 1103 68-69 0.17243960355536103 1103 70-71 0.20451217172767144 1103 72-73 0.2601547016530219 1103 74-75 0.2373155119137067 1103 76-77 0.1521973857561747 1103 78-79 0.13860516612731288 1103 80-81 0.22956909504949863 1103 82-83 0.19420286886002458 1103 84-85 0.1418758840711689 1103 86-87 0.18822649488318888 1103 88-89 0.22434394267871482 1103 90-91 0.23610979497065898 1103 92-93 0.16535076297377316 1103 94-95 0.15223293806855764 1103 96-97 0.19831975176126448 1103 98-99 0.25215477617054205 1103 100 0.24429335398839314 1104 1 0.10865927642429796 1104 2 0.031340496218582814 1104 3 0.07963665787818286 1104 4 0.047574917612564604 1104 5 0.007451423321761297 1104 6 0.038799518523653376 1104 7 0.03798401356567638 1104 8 0.05262970591382299 1104 9 0.055972092161610476 1104 10-11 0.11512800408991097 1104 12-13 0.13533023601017646 1104 14-15 0.08134677265122292 1104 16-17 0.13716006882008003 1104 18-19 0.10285395232863692 1104 20-21 0.09976531461497729 1104 22-23 0.08109949960905993 1104 24-25 0.1215367216973462 1104 26-27 0.13571301359072407 1104 28-29 0.15063242139595445 1104 30-31 0.10928086478326549 1104 32-33 0.12198082090844053 1104 34-35 0.10890505617108204 1104 36-37 0.1680787323056805 1104 38-39 0.1036696619530133 1104 40-41 0.08514001426543416 1104 42-43 0.07709682525720751 1104 44-45 0.013364267775656913 1104 46-47 0.0530274645753579 1104 48-49 0.016643680945342965 1104 50-51 0.03992544718952473 1104 52-53 0.019113071314379226 1104 54-55 -0.051881549668202354 1104 56-57 -0.01580558916513297 1104 58-59 -0.046137521636111956 1104 60-61 0.002690008391695642 1104 62-63 -0.01103256846436551 1104 64-65 0.008513156710847625 1104 66-67 6.333260120072737E-5 1104 68-69 -0.026250931269217403 1104 70-71 0.02668711701950599 1104 72-73 0.04780105746718277 1104 74-75 0.03237498983567377 1104 76-77 -0.033705204647411335 1104 78-79 -0.07429264075337016 1104 80-81 0.013816903663716573 1104 82-83 0.007513090160408353 1104 84-85 -0.0036622520578362128 1104 86-87 0.02522893355192224 1104 88-89 0.15968936891293595 1104 90-91 0.14626901969528205 1104 92-93 0.13691425656685396 1104 94-95 0.1768164575014275 1104 96-97 0.1709332546338196 1104 98-99 0.2092893225669421 1104 100 0.23026065859407652 1105 1 0.13826968474084111 1105 2 0.13632323378103095 1105 3 0.1384688675730672 1105 4 0.15963496061800697 1105 5 0.14951584623899095 1105 6 0.12523210487136538 1105 7 0.10004229284825072 1105 8 0.12051851004602554 1105 9 0.11063172549341971 1105 10-11 0.15974285600822213 1105 12-13 0.13488959924742971 1105 14-15 0.1925870519661359 1105 16-17 0.231969248703642 1105 18-19 0.20087403868246412 1105 20-21 0.22346852512950477 1105 22-23 0.2713787410318531 1105 24-25 0.27216550114722793 1105 26-27 0.282352325405661 1105 28-29 0.31242414581230094 1105 30-31 0.30297314266564257 1105 32-33 0.2706526763498047 1105 34-35 0.23855219646593184 1105 36-37 0.267847145694887 1105 38-39 0.2414486792360293 1105 40-41 0.17713298191042526 1105 42-43 0.2293709866682221 1105 44-45 0.23411091535492545 1105 46-47 0.24977879728844954 1105 48-49 0.22665012588265654 1105 50-51 0.24520353484361834 1105 52-53 0.14748306803679156 1105 54-55 0.16328595256217682 1105 56-57 0.15921613892073339 1105 58-59 0.14105834027811426 1105 60-61 0.06688791622443802 1105 62-63 0.1506043280300915 1105 64-65 0.1660840595948727 1105 66-67 0.22215091172989787 1105 68-69 0.14635034439233863 1105 70-71 0.17350216422114073 1105 72-73 0.16717114384550769 1105 74-75 0.14508937402448652 1105 76-77 0.11985569057912215 1105 78-79 0.12932530600865988 1105 80-81 0.15106042283954224 1105 82-83 0.15112241182769992 1105 84-85 0.17077467941711433 1105 86-87 0.2263162809470245 1105 88-89 0.23459595284140278 1105 90-91 0.23693713751393375 1105 92-93 0.3012248589419073 1105 94-95 0.26755474454438755 1105 96-97 0.3220480622141082 1105 98-99 0.3644766764780236 1105 100 0.3670007941165814 1106 1 0.06008617365230151 1106 2 0.04913007312418216 1106 3 0.052703673735251755 1106 4 0.0745647049890934 1106 5 0.05489336443773851 1106 6 0.021337008657830836 1106 7 0.03859485760193593 1106 8 0.04922964372922678 1106 9 0.051799266828560064 1106 10-11 0.0876002004018801 1106 12-13 0.038425234933903596 1106 14-15 0.08187857050346992 1106 16-17 0.027918122920667088 1106 18-19 0.03402087012538857 1106 20-21 -0.03178385904745795 1106 22-23 -0.008113386951890789 1106 24-25 0.0350914082630851 1106 26-27 0.054593451752928956 1106 28-29 0.060377703737920285 1106 30-31 0.06831694380553444 1106 32-33 0.05693561749307463 1106 34-35 0.020633907430323006 1106 36-37 0.004469514635687233 1106 38-39 0.01861644629206438 1106 40-41 0.0076604634295307505 1106 42-43 0.024594732506493244 1106 44-45 0.043588371480424826 1106 46-47 -0.028954908484372766 1106 48-49 -0.02739634440683858 1106 50-51 -0.029140887028738405 1106 52-53 -0.052674795514931816 1106 54-55 -0.03921066162438791 1106 56-57 -0.04767088777076367 1106 58-59 -0.03305316584456364 1106 60-61 -0.022229091845083815 1106 62-63 0.0436989615157799 1106 64-65 0.030953423626243648 1106 66-67 0.0508224031807174 1106 68-69 0.01782162140830934 1106 70-71 0.019575890308829003 1106 72-73 0.024546291974850476 1106 74-75 0.08375578675514106 1106 76-77 0.01708962207282383 1106 78-79 0.08278856993280925 1106 80-81 0.11948658389140832 1106 82-83 0.058219782179257606 1106 84-85 0.008420692621790238 1106 86-87 0.040111918890708864 1106 88-89 0.111942603707611 1106 90-91 0.12309945905334274 1106 92-93 0.13628128916592885 1106 94-95 0.1543653082823191 1106 96-97 0.13506129510163944 1106 98-99 0.164519704523844 1106 100 0.1678915452028633 1107 1 0.17717287698455664 1107 2 0.10561934304761778 1107 3 0.1325575505820069 1107 4 0.10722835213672965 1107 5 0.0756757433873716 1107 6 0.08005586986779178 1107 7 0.0412455639537086 1107 8 -0.019043299223433507 1107 9 0.07532563370012468 1107 10-11 0.06273311315935359 1107 12-13 0.03578019018031853 1107 14-15 0.02510028947251186 1107 16-17 0.04565051804630116 1107 18-19 0.10266397375959713 1107 20-21 0.07803847460939295 1107 22-23 0.03695724822550517 1107 24-25 0.07355334075526798 1107 26-27 0.12968996852205095 1107 28-29 0.12674727129966357 1107 30-31 0.1105311678969727 1107 32-33 0.14868882573205155 1107 34-35 0.09365396566390416 1107 36-37 0.0842274139131618 1107 38-39 0.07040982491818681 1107 40-41 0.056621694961933144 1107 42-43 0.10160406066351158 1107 44-45 0.07993717984880533 1107 46-47 0.03898103457613189 1107 48-49 0.036068553684394544 1107 50-51 0.015017961240424427 1107 52-53 -0.00582183530787006 1107 54-55 0.008892972582451364 1107 56-57 -0.0027895117328071706 1107 58-59 0.06922926702505094 1107 60-61 0.022480279447911755 1107 62-63 0.06885775586497545 1107 64-65 0.08915465300185588 1107 66-67 0.12713535476057558 1107 68-69 0.10606679556985199 1107 70-71 0.13197425114135797 1107 72-73 0.14078186837946305 1107 74-75 0.15803487658583748 1107 76-77 0.08830740488313893 1107 78-79 0.01920963107807694 1107 80-81 0.014532415876736593 1107 82-83 0.07137706676892464 1107 84-85 0.06845115741204211 1107 86-87 0.03492427735074699 1107 88-89 0.10143710386937244 1107 90-91 0.03247371765358764 1107 92-93 0.06569140455469125 1107 94-95 -0.023202361296025487 1107 96-97 -0.02459415620861094 1107 98-99 -0.03604915129412234 1107 100 0.05009201969870958 1108 1 -0.07091824664913204 1108 2 -0.1191316300232863 1108 3 -0.05727049679252261 1108 4 -0.16152945275708674 1108 5 -0.1768244286069489 1108 6 -0.12738882614006286 1108 7 -0.16835140644450064 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-0.09001268804904328 2214 46-47 -0.04342713443065094 2214 48-49 -0.03739615143271635 2214 50-51 0.01287811990614074 2214 52-53 0.04438095775445561 2214 54-55 0.02134907503703687 2214 56-57 0.003383319674384211 2214 58-59 0.05508323232400869 2214 60-61 0.05050619438630832 2214 62-63 0.04863316749838731 2214 64-65 0.010140860401833152 2214 66-67 0.002777586465498416 2214 68-69 -0.02043355740759978 2214 70-71 0.027570980641030474 2214 72-73 0.03346854168742652 2214 74-75 0.020173851976331036 2214 76-77 0.012052784329533495 2214 78-79 -0.0375902382590354 2214 80-81 0.01805843323347034 2214 82-83 0.00983017149946619 2214 84-85 -0.07297822753567829 2214 86-87 -0.03677925792639769 2214 88-89 -0.08116850469509274 2214 90-91 -0.2214620326568948 2214 92-93 -0.15115668489022482 2214 94-95 -0.15631414342742644 2214 96-97 -0.14861665953521808 2214 98-99 -0.22577933426192587 2214 100 -0.14867881722284082 2215 1 -0.33277971512350746 2215 2 -0.27813870946873465 2215 3 -0.25186438382551657 2215 4 -0.27755090568678753 2215 5 -0.23467678380333012 2215 6 -0.22121050501048245 2215 7 -0.13233300998304998 2215 8 -0.1802554177683291 2215 9 -0.29650404749141757 2215 10-11 -0.2938401205372685 2215 12-13 -0.33219214690321053 2215 14-15 -0.39923670881930917 2215 16-17 -0.29696614017369427 2215 18-19 -0.37350802465422106 2215 20-21 -0.34220879968003004 2215 22-23 -0.3252175975031193 2215 24-25 -0.2419233763968407 2215 26-27 -0.3664196998095548 2215 28-29 -0.3151201543009421 2215 30-31 -0.3116762121749943 2215 32-33 -0.2990309868476473 2215 34-35 -0.2859504337686971 2215 36-37 -0.47166876217038123 2215 38-39 -0.39371044143445744 2215 40-41 -0.32777781022080177 2215 42-43 -0.37587906298552554 2215 44-45 -0.3211978663206523 2215 46-47 -0.2685977966211368 2215 48-49 -0.2704050108683802 2215 50-51 -0.3388878160955002 2215 52-53 -0.4139166004426187 2215 54-55 -0.2893041308124964 2215 56-57 -0.32341852293522066 2215 58-59 -0.36578106972061164 2215 60-61 -0.3739688731520303 2215 62-63 -0.24629449031572648 2215 64-65 -0.2979405670485278 2215 66-67 -0.3210043977628487 2215 68-69 -0.26275242812109667 2215 70-71 -0.25264620699853424 2215 72-73 -0.23920434903561727 2215 74-75 -0.22253280207510073 2215 76-77 -0.3484042715628206 2215 78-79 -0.35455506295950556 2215 80-81 -0.3157655600420739 2215 82-83 -0.3179609985517686 2215 84-85 -0.3902278600751856 2215 86-87 -0.4405628383142499 2215 88-89 -0.35001908556207084 2215 90-91 -0.3707170383865588 2215 92-93 -0.42237219438081297 2215 94-95 -0.38713023817416925 2215 96-97 -0.4917820340503276 2215 98-99 -0.5957645688266915 2215 100 -0.5171822203095893 2216 1 -0.08337466191231968 2216 2 -0.062111373601133835 2216 3 -0.11124698290123902 2216 4 -0.10570347470405039 2216 5 -0.09875978699241728 2216 6 -0.06534857083980938 2216 7 -0.0331744483609171 2216 8 -0.04527331007246005 2216 9 -0.1338894577241163 2216 10-11 -0.11192230374193457 2216 12-13 -0.13536859395810552 2216 14-15 -0.18693289619719877 2216 16-17 -0.20212204028218395 2216 18-19 -0.23331645827786218 2216 20-21 -0.24058799436982525 2216 22-23 -0.21294062142849413 2216 24-25 -0.21923580496314798 2216 26-27 -0.317606386744238 2216 28-29 -0.22731578089805993 2216 30-31 -0.2705666679076799 2216 32-33 -0.1967472844577074 2216 34-35 -0.17304644447582973 2216 36-37 -0.18111274555386103 2216 38-39 -0.22296033130154314 2216 40-41 -0.1564709276198144 2216 42-43 -0.17734606395743668 2216 44-45 -0.09190399963607376 2216 46-47 -0.02687513458833024 2216 48-49 -0.02436636013211313 2216 50-51 -0.07802874881632249 2216 52-53 -0.05362564340476439 2216 54-55 -0.09408199062264089 2216 56-57 -0.14510894293672294 2216 58-59 -0.16586501505192786 2216 60-61 -0.08040166611513655 2216 62-63 -0.03922933972948073 2216 64-65 0.025145696991032196 2216 66-67 -0.049282254900802513 2216 68-69 -0.06722374813566034 2216 70-71 -0.09309262514018712 2216 72-73 0.03367523923142812 2216 74-75 0.054270152006530736 2216 76-77 -0.007852683167506314 2216 78-79 -0.025245239408853593 2216 80-81 -0.021643298381533782 2216 82-83 -0.01696201475218828 2216 84-85 -0.0075680871818590845 2216 86-87 -0.00410914872797008 2216 88-89 -0.015285072776727304 2216 90-91 0.020069474778622975 2216 92-93 0.03704354000532106 2216 94-95 0.043323402548971046 2216 96-97 -0.0650786385971962 2216 98-99 -0.04357721842231044 2216 100 -0.01878935921296332 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 39855.0 3 6948.0 4 4667.0 5 3974.0 6 3723.0 7 3986.0 8 4421.0 9 4961.0 10 5669.0 11 6102.0 12 6485.0 13 7001.0 14 7647.0 15 8022.0 16 8296.0 17 8208.0 18 8565.0 19 8615.0 20 8607.0 21 9123.0 22 10328.0 23 13033.0 24 18156.0 25 23937.0 26 26602.0 27 26639.0 28 30456.0 29 38138.0 30 47820.0 31 60441.0 32 79647.0 33 108995.0 34 154978.0 35 246317.0 36 484491.0 37 1009406.0 38 1390462.0 39 444717.0 40 538.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.401437560517806 18.2210039695784 12.968763135813457 24.40879533409034 2 15.79967207546333 19.05861931106589 39.77914810481059 25.36256050866019 3 19.018448918271016 24.33268229538511 28.571617867758953 28.077250918584923 4 12.856644609672049 15.756030499271048 36.91642438690029 34.47090050415662 5 14.13281176122864 37.48019093449452 34.44159791834074 13.9453993859361 6 33.070391494649336 35.76308884773295 17.48456290818986 13.681956749427851 7 29.77075218663685 31.05000352766224 21.295069880819813 17.884174404881097 8 26.67752381261193 35.736557716912095 19.272385499316442 18.31353297115953 9 26.218429328027625 16.660870303890018 19.55056456877915 37.57013579930321 10-11 25.5877650063507 25.873580684011998 27.517055084600617 21.021599225036685 12-13 25.92104097382129 22.88955226470811 27.809089339718547 23.380317421752046 14-15 23.090181619639022 24.67248716340584 24.62856341010913 27.608767806846007 16-17 23.435661023389557 28.041605943092858 25.177443759428236 23.34528927408935 18-19 23.37242059416549 27.305127829512394 26.636616581796375 22.685834994525745 20-21 24.262318105834314 26.414663889726235 26.39578937272204 22.92722863171741 22-23 24.041756788355304 26.140282933387894 26.251625843977433 23.56633443427937 24-25 23.757826616920394 26.296450867943054 26.285100953153055 23.660621561983493 26-27 23.56708653793456 26.483677085807194 26.258129487202908 23.691106889055334 28-29 23.5870707240474 26.47775925311482 26.381085174806874 23.554084848030907 30-31 23.683361398422416 26.631132016443537 26.072741787935712 23.61276479719833 32-33 23.588952204129708 26.451097968488536 26.418018752501354 23.541931074880406 34-35 23.699333662608723 26.352854888426563 26.38196904400302 23.565842404961693 36-37 23.918671916831542 26.367613530629324 26.08540384902998 23.628310703509154 38-39 23.636779009258426 26.688943606985088 26.197371270807423 23.476906112949063 40-41 23.61997282604042 26.48339941873163 26.297992686709087 23.59863506851886 42-43 23.600840987548445 26.43618283167942 26.31927752520599 23.64369865556614 44-45 23.513768998660833 26.545346191477048 26.35917558398798 23.581709225874143 46-47 23.649670352804527 26.519951050396877 26.33033355850577 23.500045038292825 48-49 23.682599952394785 26.47445729201688 26.188780353317437 23.654162402270895 50-51 23.555212150334313 26.539127246133294 26.234971597716765 23.670689005815625 52-53 23.599302042771573 26.460280507201738 26.379303494946388 23.561113955080305 54-55 23.583773324276656 26.66042593025977 26.300514289954506 23.455286455509068 56-57 23.458749153936665 26.558403285414194 26.31789240871278 23.664955151936365 58-59 23.512399996346897 26.2364150288595 26.501710108131803 23.749474866661796 60-61 23.629017122644935 26.544723132078214 26.222666076687272 23.60359366858958 62-63 23.452356788620214 26.599996757870215 26.30036231941965 23.647284134089922 64-65 23.53829665837343 26.48012853340725 26.241651299021683 23.73992350919764 66-67 23.394214259107283 26.474326914943152 26.27787214217896 23.853586683770605 68-69 23.468895121746012 26.504682653035715 26.340349997163138 23.686072228055142 70-71 23.48327759350045 26.316134352784736 26.388134173784074 23.812453879930736 72-73 23.467312985522554 26.455144962255257 26.305255062265953 23.772286989956235 74-75 23.47796852417994 26.54498877820575 26.230630132857826 23.74641256475649 76-77 23.501148914057026 26.356127282873054 26.2965467734968 23.84617702957312 78-79 23.530427790051263 26.48393355696546 26.3346577433004 23.650980909682882 80-81 23.496355662998088 26.351297510918855 26.440125585385115 23.71222124069794 82-83 23.395463404682857 26.363542027098745 26.51816988652975 23.72282468168865 84-85 23.450694706694364 26.3597204825571 26.29390748599148 23.895677324757056 86-87 23.541712020937606 26.377024160874164 26.398075011578538 23.683188806609692 88-89 23.35016338904739 26.4081811435235 26.399533364463807 23.842122102965305 90-91 23.358780361966044 26.330105535526986 26.459676111463086 23.851437991043884 92-93 23.476948744014557 26.36925676851371 26.45567249038648 23.69812199708525 94-95 23.414778784923648 26.16925869051846 26.519511492655717 23.896451031902174 96-97 23.429402702203635 26.156559968176946 26.541567028228265 23.872470301391154 98-99 23.234111354298484 26.390344057819902 26.52710824696099 23.848436340920625 100 23.479375843732186 25.953420241672 26.456983955136298 24.110219959459513 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 147.0 1 152.0 2 173.5 3 174.0 4 146.5 5 127.0 6 115.5 7 113.0 8 113.0 9 114.5 10 134.0 11 146.0 12 136.0 13 147.0 14 177.0 15 200.5 16 247.5 17 318.0 18 404.0 19 551.5 20 810.0 21 1179.0 22 1743.5 23 2850.0 24 4419.5 25 6505.5 26 9555.5 27 13631.5 28 19450.5 29 26879.5 30 34255.5 31 42606.0 32 52491.5 33 62931.0 34 73520.5 35 84457.0 36 95829.0 37 107831.0 38 118527.5 39 126456.5 40 134928.5 41 144028.5 42 150773.5 43 156936.0 44 161691.5 45 164061.0 46 166293.0 47 164278.0 48 161841.5 49 162510.0 50 162829.5 51 163474.0 52 158849.5 53 154990.5 54 152941.5 55 146147.0 56 135879.5 57 123591.5 58 112086.0 59 102503.0 60 93272.5 61 81293.5 62 70374.5 63 59576.5 64 48681.5 65 40000.5 66 30961.0 67 22588.5 68 17168.0 69 13469.5 70 9756.0 71 6675.5 72 4947.0 73 3637.5 74 2623.0 75 1701.5 76 989.0 77 629.5 78 405.5 79 259.0 80 178.0 81 108.0 82 63.0 83 52.0 84 45.5 85 37.0 86 30.5 87 25.5 88 19.0 89 11.0 90 9.0 91 10.5 92 8.5 93 8.5 94 7.0 95 4.0 96 4.0 97 4.0 98 5.0 99 3.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3316228216775617 2 0.11847096879069657 3 0.06536565497162541 4 0.04047967386122664 5 0.04406416838813729 6 0.009748911866183742 7 0.006963508475845529 8 0.015502368049505295 9 0.018105122037198378 10-11 0.021232993057496208 12-13 0.01579917332880363 14-15 0.01818503115085562 16-17 0.01922384962839979 18-19 0.01923526521606511 20-21 0.024109721149156983 22-23 0.01625579683541645 24-25 0.024851734347402815 26-27 0.019543486083028767 28-29 0.018927044349101457 30-31 0.01986312253765774 32-33 0.024965890224056025 34-35 0.015137069244215037 36-37 0.009840236567506306 38-39 0.012774042597493686 40-41 0.00948635334988137 42-43 0.008287716645022713 44-45 0.008926989554280663 46-47 0.008436119284671879 48-49 0.0046461441797854604 50-51 0.007089079940164055 52-53 0.007716937261756685 54-55 0.003447507474926803 56-57 0.003025130731309943 58-59 0.0031050398449671873 60-61 0.003698650403563855 62-63 0.0031735333709591103 64-65 0.0023516110590560313 66-67 0.0019977278414310944 68-69 0.0032077801339550718 70-71 0.0023744422343866724 72-73 0.002157546068745582 74-75 0.0024201045850479547 76-77 0.004292260962160523 78-79 0.0037443127542251373 80-81 0.0038128062802170607 82-83 0.002408688997382634 84-85 0.0032420268969510337 86-87 0.002408688997382634 88-89 0.5994781706566429 90-91 0.610733940094649 92-93 0.6073435105580487 94-95 0.593108272739394 96-97 0.591327441063604 98-99 0.5882680635692982 100 0.5892726352838463 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4379976.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.21165799732098 #Duplication Level Percentage of deduplicated Percentage of total 1 72.69238348270731 35.773127149610964 2 14.383977455085596 14.157187583216954 3 4.86327162083348 7.179889792576022 4 2.3679340693358535 4.661198463214422 5 1.341363494829062 3.3005360778809516 6 0.8599444853673801 2.5391576346349023 7 0.6173016420803373 2.1264906103869543 8 0.4477013214883897 1.762569945442823 9 0.3177852042790791 1.407486311063174 >10 1.906803466371031 17.39586012277227 >50 0.14382776294368868 4.857412125846036 >100 0.057007615061946695 4.619836601341372 >500 6.983796167477472E-4 0.21924758201309352 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 2.283117533064108E-5 0.0 0.0 0.0 4 0.0 2.283117533064108E-5 0.0 0.0 0.0 5 0.0 2.283117533064108E-5 0.0 0.0 0.0 6 0.0 2.283117533064108E-5 0.0 0.0 0.0 7 0.0 2.283117533064108E-5 0.0 0.0 0.0 8 0.0 2.283117533064108E-5 0.0 0.0 0.0 9 0.0 2.283117533064108E-5 0.0 0.0 0.0 10-11 0.0 2.283117533064108E-5 0.0 0.0 0.0 12-13 0.0 2.283117533064108E-5 0.0 0.0 0.0 14-15 0.0 3.424676299596162E-5 0.0 0.0 0.0 16-17 0.0 4.566235066128216E-5 0.0 5.7077938326602706E-5 0.0 18-19 0.0 4.566235066128216E-5 0.0 1.5981822731448757E-4 0.0 20-21 0.0 4.566235066128216E-5 0.0 2.9680527929833406E-4 0.0 22-23 0.0 4.566235066128216E-5 0.0 6.392729092579503E-4 0.0 24-25 0.0 4.566235066128216E-5 0.0 0.001118727591201413 0.0 26-27 0.0 4.566235066128216E-5 0.0 0.0014497796334957086 0.0 28-29 0.0 4.566235066128216E-5 0.0 0.002191792831741544 0.0 30-31 0.0 4.566235066128216E-5 0.0 0.005388157378031295 0.0 32-33 0.0 4.566235066128216E-5 0.0 0.011746639707614836 0.0 34-35 0.0 4.566235066128216E-5 0.0 0.01986312253765774 0.0 36-37 0.0 5.7077938326602706E-5 0.0 0.03605042584708227 0.0 38-39 0.0 6.849352599192324E-5 0.0 0.06584510965356888 0.0 40-41 0.0 6.849352599192324E-5 0.0 0.1025462239975744 0.0 42-43 0.0 1.1415587665320541E-4 0.0 0.13923592275391464 0.0 44-45 0.0 1.1415587665320541E-4 0.0 0.18218136355085052 0.0 46-47 0.0 1.2557146431852595E-4 0.0 0.2335743392201236 0.0 48-49 0.0 1.5981822731448757E-4 0.0 0.2912458881053229 0.0 50-51 0.0 1.5981822731448757E-4 0.0 0.3567371145412669 0.0 52-53 0.0 1.5981822731448757E-4 0.0 0.4246027832115975 0.0 54-55 0.0 1.712338149798081E-4 0.0 0.4984388042308908 0.0 56-57 0.0 1.8264940264512864E-4 0.0 0.5808022692361785 0.0 58-59 0.0 1.8264940264512864E-4 0.0 0.6757114650856535 0.0 60-61 0.0 1.8264940264512864E-4 0.0 0.7802325857493283 0.0 62-63 0.0 1.8264940264512864E-4 0.0 0.8921852539831269 0.0 64-65 0.0 1.8264940264512864E-4 0.0 1.0044689742592197 0.0 66-67 0.0 1.8264940264512864E-4 0.0 1.130873776477314 0.0 68-69 0.0 1.8264940264512864E-4 0.0 1.2727466999819177 0.0 70-71 0.0 2.0548057797576975E-4 0.0 1.4316516802831796 0.0 72-73 2.283117533064108E-5 2.2831175330641082E-4 0.0 1.601469962392488 0.0 74-75 2.283117533064108E-5 2.2831175330641082E-4 0.0 1.771984595349381 0.0 76-77 2.283117533064108E-5 2.2831175330641082E-4 0.0 1.9587321939663596 0.0 78-79 2.283117533064108E-5 2.2831175330641082E-4 0.0 2.167877175582697 0.0 80-81 2.283117533064108E-5 2.2831175330641082E-4 0.0 2.3955154092168542 0.0 82-83 2.283117533064108E-5 2.2831175330641082E-4 0.0 2.639968803482028 0.0 84-85 2.283117533064108E-5 2.2831175330641082E-4 0.0 2.886408510000968 0.0 86-87 2.283117533064108E-5 2.2831175330641082E-4 0.0 3.139583413242447 0.0 88 2.283117533064108E-5 2.2831175330641082E-4 0.0 3.34695897877066 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5475 0.0 38.208954 1 TCAACGC 5970 0.0 34.228634 4 ATCAACG 6320 0.0 32.34045 3 CAACGCA 6460 0.0 31.705786 5 GGTATCA 4140 0.0 31.182903 1 AACGCAG 6525 0.0 31.092373 6 TATCAAC 7145 0.0 28.753868 2 GTACATG 16545 0.0 28.676687 1 TACATGG 16815 0.0 26.980345 2 ACGCAGA 7725 0.0 26.080109 7 ACATGGG 17500 0.0 24.889463 3 CGCAGAG 8525 0.0 23.082094 8 CATGGGG 13980 0.0 21.43819 4 GAGTACA 10820 0.0 20.640368 1 AGTACAT 11330 0.0 18.879848 2 GCAGAGT 10280 0.0 18.639 9 GTATAGA 1555 0.0 17.876724 1 ATGGGGA 6575 0.0 16.575886 5 TAGACTG 1405 0.0 16.049055 5 GTCTAGG 1385 0.0 15.988745 1 >>END_MODULE