FastQCFastQC Report
Wed 25 May 2016
SRR1033165_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033165_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4379976
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76400.041.5176121
GGTATCA47750.040.7045061
TCAACGC88300.034.875044
CAACGCA91600.033.6703345
ATCAACG91700.033.633233
AACGCAG95850.032.163436
TATCAAC101200.030.6621422
ACGCAGA116250.026.3572627
GTACATG159500.025.7584291
TACATGG159750.024.8393732
CGCAGAG124250.024.4708238
ACATGGG167650.022.7712333
GTACTAG6350.022.2337841
GTGGTAT26100.022.178411
GCAGAGT144650.020.7270749
GAGTACA110850.020.7181021
CATGGGG134900.020.2487534
AGTACAT113050.019.5047912
TATACCG3601.10958354E-1018.283725
AGAGTAC130450.017.38156910-11
Produced by FastQC (version 0.11.5)