FastQCFastQC Report
Wed 25 May 2016
SRR1033164_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033164_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011708
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27600.041.3036271
GTATCAA46350.036.2827641
TCAACGC51200.031.8361454
ATCAACG51700.031.2603473
CAACGCA53100.030.7859735
AACGCAG55400.029.4980556
GTACATG117150.028.6298831
TATCAAC60200.027.4804362
TACATGG115550.027.3730092
ACGCAGA62250.025.4977157
ACATGGG121900.025.282853
CGCAGAG64950.024.2931618
GAGTACA83700.021.2176741
CATGGGG70850.021.0837674
TACGACG1605.438695E-520.5516265
AGTACAT82650.019.731682
CATGGGA49550.019.5292034
TACGTCG1456.626848E-419.4379925
CCGGTCG4600.019.3963349
GCAGAGT76550.019.3859
Produced by FastQC (version 0.11.5)