##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033164_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3011708
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.415378914556126	33.0	31.0	34.0	30.0	34.0
2	31.50986848658635	33.0	31.0	34.0	29.0	34.0
3	31.705297127078722	34.0	31.0	34.0	30.0	34.0
4	35.036859483057455	37.0	35.0	37.0	33.0	37.0
5	35.181168293871785	37.0	35.0	37.0	33.0	37.0
6	35.237451306700386	37.0	35.0	37.0	33.0	37.0
7	35.16708193490206	37.0	35.0	37.0	33.0	37.0
8	35.17329900508283	37.0	35.0	37.0	33.0	37.0
9	36.802811892786416	39.0	38.0	39.0	33.0	39.0
10-11	36.79840957357088	39.0	38.0	39.0	33.0	39.0
12-13	36.750497226158714	39.0	38.0	39.0	33.0	39.0
14-15	38.16752238264799	41.0	39.0	41.0	33.0	41.0
16-17	38.17042937097487	41.0	39.0	41.0	33.0	41.0
18-19	38.16591681530879	41.0	39.0	41.0	33.5	41.0
20-21	38.19295081063636	41.0	39.0	41.0	34.0	41.0
22-23	38.16362891090371	40.0	39.0	41.0	33.0	41.0
24-25	38.166490244074126	40.0	38.5	41.0	33.0	41.0
26-27	38.12764301851308	40.0	38.0	41.0	33.0	41.0
28-29	38.09810629051688	40.0	38.0	41.0	33.0	41.0
30-31	38.09167638429755	40.0	38.0	41.0	33.0	41.0
32-33	38.15472598937214	40.5	38.0	41.0	33.0	41.0
34-35	38.12220640247992	41.0	38.0	41.0	33.0	41.0
36-37	38.043830278366954	41.0	38.0	41.0	33.0	41.0
38-39	37.96042727249787	40.0	38.0	41.0	33.0	41.0
40-41	37.89712897133454	40.0	38.0	41.0	33.0	41.0
42-43	37.780409488569276	40.0	38.0	41.0	32.0	41.0
44-45	37.65758416818629	40.0	38.0	41.0	32.0	41.0
46-47	37.52813951418929	40.0	38.0	41.0	32.0	41.0
48-49	37.405212590330805	40.0	37.5	41.0	31.0	41.0
50-51	37.27142986637483	40.0	37.0	41.0	31.0	41.0
52-53	37.117505581550404	40.0	37.0	41.0	31.0	41.0
54-55	36.95047129402983	40.0	36.5	41.0	30.5	41.0
56-57	36.78651814850576	40.0	36.0	41.0	30.0	41.0
58-59	36.571140362877145	39.5	35.5	41.0	30.0	41.0
60-61	36.33226826770723	39.0	35.0	41.0	30.0	41.0
62-63	36.055577433137614	39.0	35.0	41.0	30.0	41.0
64-65	35.76257807861852	38.5	35.0	40.5	30.0	41.0
66-67	35.44673039351756	37.5	35.0	40.0	29.0	41.0
68-69	35.12118306289986	37.0	35.0	40.0	29.0	41.0
70-71	34.75890192541907	36.5	34.0	39.5	29.0	41.0
72-73	34.38064314335918	36.0	34.0	39.0	28.5	41.0
74-75	34.00293986003955	35.5	34.0	39.0	28.0	40.5
76-77	33.67596875261479	35.0	34.0	37.5	28.0	39.5
78-79	33.31209366910736	35.0	34.0	37.0	27.5	39.0
80-81	32.95803560637353	35.0	34.0	36.5	27.0	39.0
82-83	32.7524340009058	35.0	34.0	36.0	27.0	38.5
84-85	32.492732861220276	35.0	33.5	36.0	27.0	37.0
86-87	32.25482284471137	35.0	33.0	35.5	26.0	37.0
88-89	31.907485719067054	35.0	33.0	35.0	26.0	36.5
90-91	31.724374507754405	35.0	33.0	35.0	25.0	36.0
92-93	31.546629022468313	35.0	33.0	35.0	25.0	36.0
94-95	31.37161869610201	35.0	33.0	35.0	25.0	36.0
96-97	31.193624016670938	35.0	33.0	35.0	24.0	35.5
98-99	30.9914445557139	35.0	33.0	35.0	23.0	35.0
100	30.827436790020812	35.0	33.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.12864894495058365
1101	2	0.058542518978985214
1101	3	0.13774822109743212
1101	4	0.0818592374359568
1101	5	0.08293432191712213
1101	6	0.14601650102336095
1101	7	0.18316433250592468
1101	8	0.1254767647483419
1101	9	0.12140264792873978
1101	10-11	0.22225477991698028
1101	12-13	0.1928752598274741
1101	14-15	0.23000374255788358
1101	16-17	0.1883250723397154
1101	18-19	0.13223178051754303
1101	20-21	0.14666458134669824
1101	22-23	0.17949597551631769
1101	24-25	0.11043855659217172
1101	26-27	0.11513397961797978
1101	28-29	0.13195845524355576
1101	30-31	0.1707752347244167
1101	32-33	0.15963620081591756
1101	34-35	0.2079713687503002
1101	36-37	0.19986051371334668
1101	38-39	0.17698658966705239
1101	40-41	0.14899989213476772
1101	42-43	0.1959826360955006
1101	44-45	0.12142023146459735
1101	46-47	0.1226133698091445
1101	48-49	0.16811574061120638
1101	50-51	0.20025508129810987
1101	52-53	0.1319372728305055
1101	54-55	0.08621115312509886
1101	56-57	0.1171029029366224
1101	58-59	0.19825224662630347
1101	60-61	0.18919572010970853
1101	62-63	0.12430455458760292
1101	64-65	0.1205871777767129
1101	66-67	0.11416718084153388
1101	68-69	0.06562016286098782
1101	70-71	0.13783018812792136
1101	72-73	0.22003769232584602
1101	74-75	0.10515206582063996
1101	76-77	0.09688533062730187
1101	78-79	0.14184676592342527
1101	80-81	0.15840159258991093
1101	82-83	0.13683239912582934
1101	84-85	0.05208020578169226
1101	86-87	0.1422737487980683
1101	88-89	0.21376527426896885
1101	90-91	0.21734918509885404
1101	92-93	0.18580836436467507
1101	94-95	0.14936340159110273
1101	96-97	0.11824394469921273
1101	98-99	0.2350093156451436
1101	100	0.26166763664877735
1102	1	0.17460678650112982
1102	2	0.16049334351991007
1102	3	0.17382693829859974
1102	4	0.15308150915757324
1102	5	0.06396978666948172
1102	6	0.04379946057358097
1102	7	0.07021876743270639
1102	8	0.12154579197238746
1102	9	0.11637368138218562
1102	10-11	0.1392778892436013
1102	12-13	0.16288015180914073
1102	14-15	0.186825425361981
1102	16-17	0.1648830345685468
1102	18-19	0.19623203522014165
1102	20-21	0.1511346824570481
1102	22-23	0.11435179128832829
1102	24-25	0.12969327422943877
1102	26-27	0.2502734457387774
1102	28-29	0.1781495000505302
1102	30-31	0.21088936686524562
1102	32-33	0.15220929388294024
1102	34-35	0.1313479451338182
1102	36-37	0.1307719636760325
1102	38-39	0.07913849768088
1102	40-41	0.1424679687823982
1102	42-43	0.18290598377112843
1102	44-45	0.11185913291631522
1102	46-47	0.15020771785507492
1102	48-49	0.16501560583500208
1102	50-51	0.13297764531681366
1102	52-53	0.09114573515989832
1102	54-55	0.1500189710851103
1102	56-57	0.09870921636554897
1102	58-59	0.18018433971774783
1102	60-61	0.10453753381778341
1102	62-63	0.19639443640934928
1102	64-65	0.16821585898116354
1102	66-67	0.18402759253866918
1102	68-69	0.1861621624737566
1102	70-71	0.16786993959081542
1102	72-73	0.14682726004571833
1102	74-75	0.17189260563977626
1102	76-77	0.11195237568240657
1102	78-79	0.12159203512809569
1102	80-81	0.10765374440394027
1102	82-83	0.08411554006941202
1102	84-85	0.15783911614329327
1102	86-87	0.14653931244270524
1102	88-89	0.23051079535665409
1102	90-91	0.28702644119056586
1102	92-93	0.32360153081611287
1102	94-95	0.371217002108402
1102	96-97	0.3256727783492792
1102	98-99	0.3851819917893806
1102	100	0.4398257609098124
1103	1	0.18174164423891526
1103	2	0.09498692620132587
1103	3	0.23077890439231297
1103	4	0.2403518954901287
1103	5	0.2290105094643593
1103	6	0.26353677429462863
1103	7	0.24561177439768045
1103	8	0.2559233865333894
1103	9	0.24027744733553646
1103	10-11	0.25780096549403453
1103	12-13	0.2389450803353128
1103	14-15	0.25520737912704305
1103	16-17	0.2686550565988881
1103	18-19	0.31442346879094885
1103	20-21	0.30773677705197855
1103	22-23	0.30524754931344944
1103	24-25	0.3101326240674638
1103	26-27	0.3069955875259751
1103	28-29	0.361780060295807
1103	30-31	0.34510980759961285
1103	32-33	0.3015008463680431
1103	34-35	0.31091701402714733
1103	36-37	0.2910097740498614
1103	38-39	0.311679763582994
1103	40-41	0.3395037703653969
1103	42-43	0.24482723868812428
1103	44-45	0.21499385789455516
1103	46-47	0.3041363187243249
1103	48-49	0.30787671749011025
1103	50-51	0.26066841713935673
1103	52-53	0.18864780056139807
1103	54-55	0.2545311048065244
1103	56-57	0.18499207549150753
1103	58-59	0.1264311841167327
1103	60-61	0.0835369037223117
1103	62-63	0.1199185339746407
1103	64-65	0.2630522299955871
1103	66-67	0.29930950276407486
1103	68-69	0.2382606627691075
1103	70-71	0.32535688907707083
1103	72-73	0.33233951035871456
1103	74-75	0.28124804324757235
1103	76-77	0.24564405541629242
1103	78-79	0.25539942402794225
1103	80-81	0.3077600089693533
1103	82-83	0.3153956472243564
1103	84-85	0.3113028308874348
1103	86-87	0.29338218571449914
1103	88-89	0.33645177360549283
1103	90-91	0.3816975743923372
1103	92-93	0.3582070254672445
1103	94-95	0.33420364668035774
1103	96-97	0.2985919660430376
1103	98-99	0.3100029428851343
1103	100	0.35867637522443374
1104	1	0.015573263896193623
1104	2	-0.06299476724579378
1104	3	0.0046687046279458855
1104	4	-0.017065862897254647
1104	5	-0.006778853404256324
1104	6	0.010062434812830645
1104	7	8.144836427419477E-4
1104	8	0.002064014183531526
1104	9	0.0636570763133193
1104	10-11	-8.436722382327844E-4
1104	12-13	0.05278940553569811
1104	14-15	0.04605893319768484
1104	16-17	0.049182314434872865
1104	18-19	-0.004157681299517435
1104	20-21	-0.030850431755403918
1104	22-23	0.030930747085605503
1104	24-25	-0.03871159499166055
1104	26-27	-0.013944389803114632
1104	28-29	0.04595005398581975
1104	30-31	0.0496114321025658
1104	32-33	0.006709528767153472
1104	34-35	1.2684968363174676E-4
1104	36-37	0.07062803481036184
1104	38-39	0.03540043095596701
1104	40-41	-0.006953651064605992
1104	42-43	0.025792572949733028
1104	44-45	-0.06524457113668802
1104	46-47	-0.08911372476110557
1104	48-49	-0.10744475300403167
1104	50-51	-0.025184181014779483
1104	52-53	-0.09586403299869772
1104	54-55	-0.07826769532057654
1104	56-57	-0.08344561059417543
1104	58-59	-0.09658556627518067
1104	60-61	-0.07172806062613546
1104	62-63	-0.14685471080076695
1104	64-65	-0.16917597416561847
1104	66-67	-0.17874107853266707
1104	68-69	-0.22260066496285447
1104	70-71	-0.1321249223168337
1104	72-73	-0.11210601415136523
1104	74-75	-0.10518580832231095
1104	76-77	-0.20656897920807182
1104	78-79	-0.13542401885256794
1104	80-81	-0.037840348791711165
1104	82-83	-0.01018424693330644
1104	84-85	-0.017961492064486606
1104	86-87	0.04189461041647036
1104	88-89	0.15148604532150856
1104	90-91	0.14466002269450584
1104	92-93	0.01998164466377972
1104	94-95	-0.07179499211202156
1104	96-97	-0.06856893263992347
1104	98-99	-7.715981134452932E-4
1104	100	0.1115294633613253
1105	1	-0.03807554714303407
1105	2	-0.11104430439513635
1105	3	-0.09004944089527456
1105	4	-0.05986585096528074
1105	5	-0.11710089852741135
1105	6	-0.08867327349358334
1105	7	-0.15393799322508528
1105	8	-0.15547362486483252
1105	9	-0.08767117364157428
1105	10-11	-0.1326769521608071
1105	12-13	-0.09247065548721167
1105	14-15	-0.15873521234790644
1105	16-17	-0.12380380822785497
1105	18-19	-0.1989532862328076
1105	20-21	-0.1144116412097489
1105	22-23	-0.07567529716170895
1105	24-25	-0.08135730641156869
1105	26-27	-0.05257069996350339
1105	28-29	-0.010464395133134019
1105	30-31	-0.040169187435992626
1105	32-33	0.003215931740044198
1105	34-35	-0.041265357496797606
1105	36-37	-0.08753341738500353
1105	38-39	-0.0696910195179683
1105	40-41	-0.10311349865854424
1105	42-43	-0.11281758969172273
1105	44-45	-0.14354856687597817
1105	46-47	-0.07920128180824548
1105	48-49	-0.09547580595691585
1105	50-51	-0.13324896560646238
1105	52-53	-0.1689463675301326
1105	54-55	-0.2690526511774678
1105	56-57	-0.25744442299024684
1105	58-59	-0.19796347580639662
1105	60-61	-0.17907757434655736
1105	62-63	-0.17424060253015483
1105	64-65	-0.1619226930291333
1105	66-67	-0.12346099520773635
1105	68-69	-0.1854487869307917
1105	70-71	-0.17711088464371016
1105	72-73	-0.1863681067165217
1105	74-75	-0.1900502126652981
1105	76-77	-0.24241750698729447
1105	78-79	-0.23243728198337976
1105	80-81	-0.2391187579120455
1105	82-83	-0.18001067612338062
1105	84-85	-0.17997758178793077
1105	86-87	-0.1060512832767131
1105	88-89	-0.14607114268595112
1105	90-91	-0.0842420371654562
1105	92-93	-0.11055985963866632
1105	94-95	-0.10043270496873902
1105	96-97	-0.06320912676645207
1105	98-99	-0.04936232055074896
1105	100	0.09712305920767506
1106	1	0.06908020331918863
1106	2	0.028740977679319712
1106	3	0.03638748431357186
1106	4	-0.004368365513130357
1106	5	0.006577019230057601
1106	6	0.020632491975312917
1106	7	-0.011715204513635058
1106	8	0.03426009313914591
1106	9	0.005278504366806658
1106	10-11	-0.04156039891923058
1106	12-13	-0.042477675458734154
1106	14-15	0.022337169606757357
1106	16-17	-0.07198283455582555
1106	18-19	0.0047367647720477635
1106	20-21	-0.0015847353643252404
1106	22-23	-0.04380745228998251
1106	24-25	-0.017780507972702253
1106	26-27	0.04119177771363525
1106	28-29	0.11918505941607549
1106	30-31	0.06740153952073058
1106	32-33	0.03621331779216774
1106	34-35	0.028143445884609264
1106	36-37	0.0479824478993649
1106	38-39	0.03714647736246235
1106	40-41	0.05272258143694586
1106	42-43	0.061061626740631425
1106	44-45	0.0578464343292211
1106	46-47	0.012468887407770524
1106	48-49	0.04816594903200411
1106	50-51	0.0793002547552959
1106	52-53	-0.02230181476527804
1106	54-55	0.06601401029362819
1106	56-57	0.007200893444384349
1106	58-59	0.005160044657763763
1106	60-61	0.022580725366765364
1106	62-63	0.06470259529096012
1106	64-65	0.051220027255439504
1106	66-67	0.09255307190299078
1106	68-69	0.016217791986228747
1106	70-71	-0.03595757570378311
1106	72-73	0.0043937446165429606
1106	74-75	-4.009970727523182E-4
1106	76-77	-0.08167951859979894
1106	78-79	-0.09660038685729688
1106	80-81	-0.02939520002787077
1106	82-83	-0.09229278557722864
1106	84-85	-0.03599298348647295
1106	86-87	-0.00171953449373774
1106	88-89	0.043603852463032666
1106	90-91	0.033309839977903266
1106	92-93	0.02148362333408471
1106	94-95	-0.010909105222530968
1106	96-97	0.07915686242338893
1106	98-99	0.127442652444671
1106	100	0.135172841712496
1107	1	0.10141711897326999
1107	2	0.1450298497690916
1107	3	0.10310432002251346
1107	4	0.0489810458852844
1107	5	0.03876339921973937
1107	6	0.03729058310318578
1107	7	0.06300570521833038
1107	8	0.11391257977428637
1107	9	0.14835987096238767
1107	10-11	0.1337344583544109
1107	12-13	0.09266289916799764
1107	14-15	0.1286009371990957
1107	16-17	0.09278537048545132
1107	18-19	0.17954969388787134
1107	20-21	0.1560944527304855
1107	22-23	0.1845905371813643
1107	24-25	0.20573498921021383
1107	26-27	0.24510367882069772
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2216	34-35	-0.227429575352339
2216	36-37	-0.27449715943919983
2216	38-39	-0.20097135442512837
2216	40-41	-0.2294383220453966
2216	42-43	-0.22731845188517497
2216	44-45	-0.18146478122170606
2216	46-47	-0.10695974690239751
2216	48-49	-0.11534266954188155
2216	50-51	-0.13055195107932605
2216	52-53	-0.10433756706142105
2216	54-55	-0.16931612542199304
2216	56-57	-0.27931266822527334
2216	58-59	-0.24210846611670434
2216	60-61	-0.19634780290662235
2216	62-63	-0.1480871642876309
2216	64-65	-0.14305814030489472
2216	66-67	-0.1968968580571442
2216	68-69	-0.2525345697232595
2216	70-71	-0.2671512145440147
2216	72-73	-0.18730450825393063
2216	74-75	-0.0996904117252555
2216	76-77	-0.16320593467304434
2216	78-79	-0.18898715111375708
2216	80-81	-0.15963811334603406
2216	82-83	-0.16196912243765382
2216	84-85	-0.18003134606943405
2216	86-87	-0.16866376078748857
2216	88-89	-0.0771785288530431
2216	90-91	-0.1179635087548796
2216	92-93	-0.08016897240845111
2216	94-95	-0.06480439910298941
2216	96-97	-0.07317284800057422
2216	98-99	-0.07528571372409232
2216	100	-0.04092480237446949
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	34082.0
3	6057.0
4	4061.0
5	3379.0
6	2914.0
7	3452.0
8	4110.0
9	4346.0
10	4508.0
11	4740.0
12	5421.0
13	5957.0
14	6411.0
15	6730.0
16	7094.0
17	7343.0
18	6728.0
19	6767.0
20	6803.0
21	6977.0
22	8235.0
23	11776.0
24	18607.0
25	24642.0
26	24781.0
27	20996.0
28	22329.0
29	27612.0
30	34426.0
31	42173.0
32	54931.0
33	74145.0
34	105237.0
35	159772.0
36	301616.0
37	633712.0
38	952572.0
39	355583.0
40	683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.9275423493067	19.39687697704224	13.039958449422533	23.63562222422853
2	15.560601023868092	19.875905857323318	40.34243068944884	24.221062429359748
3	18.27632171748517	25.89760236241925	29.24271279515289	26.58336312494269
4	12.146073219300824	16.620545449411985	38.90508197020805	32.32829936107914
5	12.782973397261928	38.859177489551364	35.424465908992765	12.933383204193936
6	30.9851419129289	38.17908072725727	17.79497008724112	13.04080727257271
7	27.91641803732412	32.48525408009468	22.31149080976294	17.286837072818262
8	25.504175002058943	36.54939998459115	20.006734731288205	17.939690282061704
9	25.541191672763393	16.91257809648646	20.492272296001	37.05395793474915
10-11	24.115177163831085	27.218778094127593	28.51150233985579	20.154542402185527
12-13	24.70402929295965	24.68823843236439	28.369783764836725	22.237948509839235
14-15	22.08945687219263	26.321546128145613	25.469980759909678	26.119016239752078
16-17	21.81455195707797	29.58059495515727	26.14723786001588	22.457615227748875
18-19	21.880851726618957	28.646931775291705	27.96660589768527	21.50561060040407
20-21	23.118299925539045	27.850107639587723	27.42642414049407	21.605168294379162
22-23	22.617659570723266	27.68683181840371	27.490717313169842	22.20479129770318
24-25	22.36434012624219	27.857956195676557	27.4788742521692	22.298829425912047
26-27	21.981796245072875	27.931940658960386	27.505407425832836	22.580855670133907
28-29	22.29316993993134	27.736229248913407	27.773623755142367	22.196977056012884
30-31	22.459417802930545	28.252063773138886	27.219554509946658	22.068963913983907
32-33	22.03015490945141	27.874468295331795	27.811083037676614	22.28429375754018
34-35	22.367611591483637	27.62495797028655	27.776870996799513	22.230559441430298
36-37	22.646414024452202	27.90631415150165	27.12942307066486	22.317848753381288
38-39	22.24759133628243	28.1991069023952	27.47897348568749	22.07432827563488
40-41	22.16599018871759	27.818419216644845	27.712340080181146	22.303250514456415
42-43	22.076668855529256	27.849886641269215	27.63567143152444	22.437773071677086
44-45	22.03160310608414	27.947601225923307	27.67225163870694	22.348544029285613
46-47	22.24548323720939	27.98486058250828	27.5408259038382	22.228830276444132
48-49	22.220012239394045	27.88415210983445	27.48917763292244	22.40665801784906
50-51	22.19928434715273	27.778401310170693	27.42801300076108	22.5943013419155
52-53	22.218115725827943	27.776729016232338	27.627583502311232	22.377571755628487
54-55	22.23719573623977	28.25816987170335	27.40739683106799	22.09723756098889
56-57	22.132791168639184	27.930363978853983	27.399986917387952	22.536857935118878
58-59	22.106915524151376	27.49733533003722	27.730066442424867	22.66568270338654
60-61	22.29161879614663	27.936250413402064	27.43905557053469	22.333075219916616
62-63	22.046969174497324	27.90436090155659	27.552821768131935	22.49584815581415
64-65	22.12885833034769	27.806841006191657	27.45351110178432	22.610789561676338
66-67	21.996190455951588	27.781547402724723	27.474356635149576	22.747905506174114
68-69	22.36006788370781	27.9489019368639	27.374427260269467	22.316602919158818
70-71	22.34613952991077	27.694237128528933	27.53714669178697	22.42247664977333
72-73	22.101312006354	27.76811160139286	27.580772248540413	22.549804143712727
74-75	22.095597177292493	27.915750432280007	27.437772846921828	22.550879543505673
76-77	22.184414632251226	27.803548552693357	27.393898380724625	22.61813843433079
78-79	22.32635460599159	27.90671882712355	27.421761389983313	22.34516517690155
80-81	22.272525704278724	27.670287266659237	27.547993855113024	22.50919317394901
82-83	22.094264758887576	27.78785075014913	27.658751418872374	22.45913307209092
84-85	22.080834607703466	27.736630652255588	27.463355667392797	22.719179072648146
86-87	22.375140164634715	27.667179012610088	27.477066459204746	22.480614363550455
88-89	22.119558808447408	27.67141738983156	27.612772466718045	22.596251335002986
90-91	22.113326372937983	27.516007517226974	27.722514094800584	22.648152015034455
92-93	22.22050724383485	27.73160142788655	27.491760586789876	22.556130741488722
94-95	22.1282612090556	27.452939462038824	27.73438824432313	22.684411084582447
96-97	22.255629202339968	27.537637968025724	27.531024120834317	22.67570870879999
98-99	21.950648814470593	27.682889923334074	27.583617033784847	22.782844228410486
100	22.086616448326783	27.43119195571273	27.634383495889907	22.84780810007058
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	547.0
1	497.5
2	411.0
3	347.5
4	280.5
5	228.5
6	206.0
7	190.5
8	209.5
9	230.0
10	214.0
11	231.0
12	263.5
13	278.5
14	307.0
15	359.0
16	452.5
17	612.5
18	832.0
19	1142.5
20	1664.0
21	2249.0
22	3312.0
23	5190.5
24	7485.5
25	10257.5
26	14220.5
27	19574.0
28	26099.0
29	33540.0
30	40109.0
31	46491.0
32	54516.5
33	62988.0
34	70391.5
35	78215.5
36	86781.5
37	94375.5
38	100449.0
39	103822.0
40	107331.5
41	111866.5
42	114520.0
43	116833.5
44	116137.0
45	114760.0
46	116124.5
47	114622.0
48	110447.5
49	106840.5
50	104614.0
51	100215.0
52	90944.0
53	83588.0
54	80357.5
55	77290.0
56	71118.0
57	62760.0
58	56125.5
59	50370.5
60	44262.5
61	37474.5
62	31953.0
63	26997.0
64	22057.0
65	17929.5
66	14081.5
67	10831.0
68	8048.5
69	6040.0
70	4446.0
71	3128.5
72	2320.5
73	1708.5
74	1255.0
75	958.5
76	664.5
77	434.5
78	291.5
79	185.5
80	129.0
81	89.0
82	65.0
83	44.5
84	25.0
85	16.5
86	16.5
87	15.0
88	12.5
89	10.5
90	8.0
91	7.5
92	6.5
93	6.5
94	5.0
95	2.5
96	3.5
97	3.5
98	1.5
99	2.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33419574540426894
2	0.11647875557656985
3	0.06348557031425357
4	0.03861596144114901
5	0.04203594770807794
6	0.009429865046677833
7	0.006541138782378637
8	0.01500809507429007
9	0.01756478383694568
10-11	0.0207523438527241
12-13	0.01568877195265942
14-15	0.018179053214986314
16-17	0.019042350719259634
18-19	0.019175165719917068
20-21	0.024371552620639187
22-23	0.015174113825111864
24-25	0.024886210748186748
26-27	0.01895934134384874
28-29	0.01894273946876656
30-31	0.019822638848122062
32-33	0.02508543324917289
34-35	0.01479227069822174
36-37	0.009363457546349116
38-39	0.0129826663142642
40-41	0.009197438795527322
42-43	0.00831753941617182
44-45	0.008749188168308481
46-47	0.007686668163049008
48-49	0.004017653769887386
50-51	0.0063751200315568446
52-53	0.007288223161076705
54-55	0.003121152515449705
56-57	0.0025566887626556093
58-59	0.002589892512819968
60-61	0.003718820018408159
62-63	0.003137754390531884
64-65	0.0024902812623268924
66-67	0.002042030635108052
68-69	0.002888726264299195
70-71	0.002224651261012024
72-73	0.0020254287600258722
74-75	0.002008826884943693
76-77	0.0037852275187368763
78-79	0.003436588142011111
80-81	0.0032705693911893184
82-83	0.0024404756370803543
84-85	0.0029717356397100914
86-87	0.002357466261669458
88-89	0.6065993117526666
90-91	0.6170252893042751
92-93	0.6133728767861958
94-95	0.6006724423483285
96-97	0.5973022617066461
98-99	0.5956752779485927
100	0.5970366317053314
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3011708.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.342262386405594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.55505511985379	39.97148105169621
2	14.396078275780075	15.646309259953487
3	5.242797068783563	8.547163618515437
4	2.373650335475642	5.1595811737598805
5	1.258696142787831	3.4200198028066473
6	0.7573169939218685	2.469259127603167
7	0.5050363758626106	1.9211373476263744
8	0.32264368754774675	1.4026550340829695
9	0.24700598663783768	1.2080577723197732
>10	1.2018530004720993	11.696826556414718
>50	0.08927719318359778	3.33984446023703
>100	0.04955028868340737	4.872408353229824
>500	0.0010395310098939607	0.3452564417543779
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.320375016435856E-5	0.0
5	0.0	0.0	0.0	3.320375016435856E-5	0.0
6	0.0	0.0	0.0	3.320375016435856E-5	0.0
7	0.0	0.0	0.0	3.320375016435856E-5	0.0
8	0.0	0.0	0.0	3.320375016435856E-5	0.0
9	0.0	0.0	0.0	3.320375016435856E-5	0.0
10-11	0.0	0.0	0.0	3.320375016435856E-5	0.0
12-13	0.0	0.0	0.0	4.980562524653784E-5	0.0
14-15	0.0	0.0	0.0	6.640750032871713E-5	0.0
16-17	0.0	0.0	0.0	6.640750032871713E-5	0.0
18-19	0.0	0.0	0.0	6.640750032871713E-5	0.0
20-21	0.0	0.0	0.0	6.640750032871713E-5	0.0
22-23	0.0	0.0	0.0	8.300937541089641E-5	0.0
24-25	0.0	0.0	0.0	2.1582437606833065E-4	0.0
26-27	0.0	0.0	0.0	4.316487521366613E-4	0.0
28-29	0.0	0.0	0.0	0.001294946256409984	0.0
30-31	0.0	0.0	0.0	0.005179785025639936	0.0
32-33	0.0	0.0	0.0	0.014742465072975202	0.0
34-35	0.0	0.0	0.0	0.027144065759363126	0.0
36-37	0.0	0.0	0.0	0.05051950587507155	0.0
38-39	0.0	0.0	0.0	0.09169215607887617	0.0
40-41	0.0	0.0	0.0	0.1407008913214694	0.0
42-43	0.0	0.0	0.0	0.1885640971833923	0.0
44-45	0.0	0.0	0.0	0.24178970869685906	0.0
46-47	0.0	0.0	0.0	0.31053807341216344	0.0
48-49	0.0	0.0	0.0	0.3879360150452833	0.0
50-51	0.0	0.0	0.0	0.47532828547787503	0.0
52-53	0.0	0.0	0.0	0.5589021246415655	0.0
54-55	0.0	0.0	0.0	0.6487348707112376	0.0
56-57	0.0	0.0	0.0	0.7546880374857058	0.0
58-59	0.0	0.0	0.0	0.8772430793423532	0.0
60-61	0.0	0.0	0.0	1.0060736299800646	0.0
62-63	0.0	0.0	0.0	1.138373972509951	0.0
64-65	0.0	0.0	0.0	1.2759371094408887	0.0
66-67	0.0	0.0	0.0	1.4342857939747147	0.0
68-69	0.0	0.0	0.0	1.6178859305085354	0.0
70-71	0.0	0.0	0.0	1.8181875533750285	0.0
72-73	3.320375016435856E-5	0.0	0.0	2.0285997181665687	0.0
74-75	3.320375016435856E-5	0.0	0.0	2.2360401473183984	0.0
76-77	3.320375016435856E-5	0.0	0.0	2.457110715912698	0.0
78-79	3.320375016435856E-5	0.0	0.0	2.7094924209119875	0.0
80-81	3.320375016435856E-5	0.0	0.0	2.981613755383988	0.0
82-83	3.320375016435856E-5	0.0	0.0	3.2737735530801793	0.0
84-85	3.320375016435856E-5	0.0	0.0	3.566066165777028	0.0
86-87	3.320375016435856E-5	0.0	0.0	3.872138334792085	0.0
88	3.320375016435856E-5	0.0	0.0	4.1252671241700725	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2760	0.0	41.303627	1
GTATCAA	4635	0.0	36.282764	1
TCAACGC	5120	0.0	31.836145	4
ATCAACG	5170	0.0	31.260347	3
CAACGCA	5310	0.0	30.785973	5
AACGCAG	5540	0.0	29.498055	6
GTACATG	11715	0.0	28.629883	1
TATCAAC	6020	0.0	27.480436	2
TACATGG	11555	0.0	27.373009	2
ACGCAGA	6225	0.0	25.497715	7
ACATGGG	12190	0.0	25.28285	3
CGCAGAG	6495	0.0	24.293161	8
GAGTACA	8370	0.0	21.217674	1
CATGGGG	7085	0.0	21.083767	4
TACGACG	160	5.438695E-5	20.551626	5
AGTACAT	8265	0.0	19.73168	2
CATGGGA	4955	0.0	19.529203	4
TACGTCG	145	6.626848E-4	19.437992	5
CCGGTCG	460	0.0	19.396334	9
GCAGAGT	7655	0.0	19.385	9
>>END_MODULE