FastQCFastQC Report
Wed 25 May 2016
SRR1033164_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033164_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3011708
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66290.2201076598395329No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55150.18311868215643748No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45200.057.2606351
GTATCAA90650.043.657621
ATCAACG107450.036.4036253
TCAACGC108000.036.218244
CAACGCA109650.035.6732335
AACGCAG114800.034.100836
TATCAAC115600.034.0410232
ACGCAGA134250.028.8452827
CGCAGAG137500.028.1274388
GTACATG115700.027.4131161
TACATGG113150.027.0496332
ACATGGG116200.025.6109313
GCAGAGT150900.025.1625799
GTGGTAT19500.023.8908981
CCGATCG1050.002338394322.3776119
GAGTACT95450.021.61332912-13
CAGAGTA149100.020.78596510-11
TGGTATC19750.020.4722842
CATGGGG68600.020.4230184
AGTACTT96850.020.40325412-13
Produced by FastQC (version 0.11.5)