##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033164_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3011708 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.724742571324974 34.0 31.0 34.0 31.0 34.0 2 32.81412573861742 34.0 31.0 34.0 31.0 34.0 3 32.91924084273774 34.0 31.0 34.0 31.0 34.0 4 36.3554338601219 37.0 37.0 37.0 35.0 37.0 5 36.29127657794182 37.0 37.0 37.0 35.0 37.0 6 36.37883320693773 37.0 37.0 37.0 35.0 37.0 7 36.35762431152024 37.0 37.0 37.0 35.0 37.0 8 36.34977660516889 37.0 37.0 37.0 35.0 37.0 9 38.16575245674547 39.0 39.0 39.0 37.0 39.0 10-11 38.15142852494332 39.0 39.0 39.0 37.0 39.0 12-13 38.11130876565723 39.0 39.0 39.0 37.0 39.0 14-15 39.637949462564094 41.0 40.0 41.0 37.0 41.0 16-17 39.64482114467937 41.0 40.0 41.0 37.0 41.0 18-19 39.662341103453585 41.0 40.0 41.0 37.0 41.0 20-21 39.653923952786926 41.0 40.0 41.0 37.0 41.0 22-23 39.582153880787914 41.0 40.0 41.0 37.0 41.0 24-25 39.53419936461303 41.0 39.5 41.0 37.0 41.0 26-27 39.43465701190155 41.0 39.0 41.0 36.0 41.0 28-29 39.34692722534854 41.0 39.0 41.0 36.0 41.0 30-31 39.246475587938804 41.0 39.0 41.0 36.0 41.0 32-33 39.277635315243046 41.0 39.0 41.0 35.5 41.0 34-35 39.222253618212655 41.0 39.0 41.0 35.0 41.0 36-37 39.14065573422124 41.0 39.0 41.0 35.0 41.0 38-39 39.054802789646274 41.0 39.0 41.0 35.0 41.0 40-41 38.94192332058752 41.0 39.0 41.0 35.0 41.0 42-43 38.820538212867916 41.0 39.0 41.0 35.0 41.0 44-45 38.66311209453241 41.0 39.0 41.0 35.0 41.0 46-47 38.42098486971513 41.0 38.0 41.0 35.0 41.0 48-49 38.20655787347246 41.0 38.0 41.0 34.0 41.0 50-51 38.0565244040923 40.0 38.0 41.0 34.0 41.0 52-53 37.915412782381296 40.0 38.0 41.0 34.0 41.0 54-55 37.76397363223792 40.0 37.0 41.0 34.0 41.0 56-57 37.597699212539865 40.0 37.0 41.0 33.0 41.0 58-59 37.39141809232502 40.0 36.5 41.0 33.0 41.0 60-61 37.18280772903615 39.5 36.0 41.0 33.0 41.0 62-63 36.930271626598596 39.0 35.5 41.0 33.0 41.0 64-65 36.66239771584762 39.0 35.0 41.0 32.5 41.0 66-67 36.35704208376111 38.5 35.0 41.0 32.0 41.0 68-69 36.01848801411026 37.5 35.0 40.0 32.0 41.0 70-71 35.67365096483457 37.0 35.0 39.5 31.0 41.0 72-73 35.313552641889586 36.5 35.0 39.0 31.0 41.0 74-75 34.971831432529314 36.0 35.0 39.0 31.0 41.0 76-77 34.64124626291792 35.5 35.0 38.5 31.0 40.0 78-79 34.314815380508335 35.0 35.0 37.0 31.0 39.0 80-81 33.99000633527553 35.0 34.5 37.0 30.0 39.0 82-83 33.69376513260914 35.0 34.0 36.0 30.0 38.5 84-85 33.43998654584043 35.0 34.0 36.0 30.0 37.0 86-87 33.23331577961741 35.0 34.0 36.0 30.0 37.0 88-89 33.03442448603915 35.0 34.0 35.0 30.0 36.5 90-91 32.85597441717457 35.0 34.0 35.0 29.0 36.0 92-93 32.70532750851012 35.0 34.0 35.0 29.0 36.0 94-95 32.564925118902636 35.0 34.0 35.0 29.0 36.0 96-97 32.45151223159748 35.0 34.0 35.0 29.0 36.0 98-99 32.351492740996136 35.0 34.0 35.0 29.0 35.0 100 32.26156918266977 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07529153713445425 1101 2 0.11060107682514797 1101 3 0.12201988153962873 1101 4 0.06266363929808705 1101 5 0.07652005553421759 1101 6 0.09059152313881924 1101 7 0.09955236303802195 1101 8 0.14032887011745743 1101 9 0.1640152172841951 1101 10-11 0.1530086730604694 1101 12-13 0.18048976755698476 1101 14-15 0.15643111160417078 1101 16-17 0.1775390008017652 1101 18-19 0.20329086737025648 1101 20-21 0.18844169897353424 1101 22-23 0.2166958341124925 1101 24-25 0.17036321982448754 1101 26-27 0.21915380206430513 1101 28-29 0.23059058043412506 1101 30-31 0.18814099839060106 1101 32-33 0.15450461719225217 1101 34-35 0.20718982733543356 1101 36-37 0.2689599161228955 1101 38-39 0.2564594595786076 1101 40-41 0.3247665000630917 1101 42-43 0.330831318387915 1101 44-45 0.2871207228511281 1101 46-47 0.32868137913804674 1101 48-49 0.37273169957420293 1101 50-51 0.2456977731648351 1101 52-53 0.3388026770674344 1101 54-55 0.3476458548253376 1101 56-57 0.3581948441170155 1101 58-59 0.30502792644116283 1101 60-61 0.27900229882791905 1101 62-63 0.26542286301064166 1101 64-65 0.31769304485403893 1101 66-67 0.29830843310682553 1101 68-69 0.3627941321096131 1101 70-71 0.2764129363382182 1101 72-73 0.3594599075814884 1101 74-75 0.3272292646132442 1101 76-77 0.272725231532263 1101 78-79 0.3185368316683963 1101 80-81 0.2301612765051786 1101 82-83 0.25415711220500015 1101 84-85 0.33882630512994893 1101 86-87 0.3429420060787223 1101 88-89 0.3044725704706934 1101 90-91 0.3196930287285298 1101 92-93 0.32059442565480367 1101 94-95 0.4505189318079843 1101 96-97 0.4381091447550318 1101 98-99 0.4354612239463975 1101 100 0.4914488304384719 1102 1 0.08080288784771739 1102 2 0.1288064385498373 1102 3 0.09060123505368267 1102 4 0.0540913930467255 1102 5 0.06771566337116042 1102 6 0.08200376916461494 1102 7 0.10263229078142899 1102 8 0.12873285287662384 1102 9 0.1546523121331873 1102 10-11 0.1436341371173313 1102 12-13 0.137704547638684 1102 14-15 0.17054384429050629 1102 16-17 0.12441106960718429 1102 18-19 0.16410555384169356 1102 20-21 0.1467148811394665 1102 22-23 0.1760220141562101 1102 24-25 0.16955470354701419 1102 26-27 0.188749266666278 1102 28-29 0.16811360705288791 1102 30-31 0.24204631293421386 1102 32-33 0.1992399332584469 1102 34-35 0.17240377281968478 1102 36-37 0.16403795286398548 1102 38-39 0.1420838944865821 1102 40-41 0.16251589244231468 1102 42-43 0.17842259308513775 1102 44-45 0.26706019103961864 1102 46-47 0.22465392952874907 1102 48-49 0.2683259555136672 1102 50-51 0.20405507297893166 1102 52-53 0.25399199816752116 1102 54-55 0.22655838280972773 1102 56-57 0.2069994806441926 1102 58-59 0.26870014581247403 1102 60-61 0.27395970428250394 1102 62-63 0.26528576064275455 1102 64-65 0.15975475901193192 1102 66-67 0.19308254197667907 1102 68-69 0.18782026027295018 1102 70-71 0.15068313350246854 1102 72-73 0.16843096077203512 1102 74-75 0.1539775723917316 1102 76-77 0.09892724452867441 1102 78-79 0.10366111901804231 1102 80-81 0.13948762102474888 1102 82-83 0.003953162153031542 1102 84-85 0.09496689344596376 1102 86-87 0.13281588599819827 1102 88-89 0.11847505734552044 1102 90-91 0.026639595694639695 1102 92-93 0.09983435456364731 1102 94-95 0.10561801205904686 1102 96-97 0.1111104812387822 1102 98-99 0.062170480459165844 1102 100 0.044822183095369894 1103 1 0.13200378104846777 1103 2 0.12696505455321017 1103 3 0.12244223420702127 1103 4 0.08023392214565916 1103 5 0.07073525986412221 1103 6 0.1046369374631837 1103 7 0.1241197533161369 1103 8 0.12496617083140649 1103 9 0.1491697755924335 1103 10-11 0.12602758562734806 1103 12-13 0.11937937533591736 1103 14-15 0.16593198720080693 1103 16-17 0.17244642759568762 1103 18-19 0.17313158040544607 1103 20-21 0.13678455826734393 1103 22-23 0.1677703992946178 1103 24-25 0.19709895027664714 1103 26-27 0.2143720296224103 1103 28-29 0.18997928058099944 1103 30-31 0.21091898172898738 1103 32-33 0.22943174233610364 1103 34-35 0.2567231342166636 1103 36-37 0.2065876716551216 1103 38-39 0.22597898894169077 1103 40-41 0.29475148689310515 1103 42-43 0.24311662673911627 1103 44-45 0.24414194837520853 1103 46-47 0.24134047217415855 1103 48-49 0.22793887376884925 1103 50-51 0.267634655152996 1103 52-53 0.28439623959587834 1103 54-55 0.2530658014039915 1103 56-57 0.30811164355905163 1103 58-59 0.3767612347868763 1103 60-61 0.35110427191222016 1103 62-63 0.23442705118394258 1103 64-65 0.23310403753325915 1103 66-67 0.3103042957325428 1103 68-69 0.36611832161924696 1103 70-71 0.3359676943644061 1103 72-73 0.4163113339470499 1103 74-75 0.39015557880335194 1103 76-77 0.28801802495895856 1103 78-79 0.21696985350304487 1103 80-81 0.30818179313121163 1103 82-83 0.2820767801899251 1103 84-85 0.2583703244262523 1103 86-87 0.274102897560347 1103 88-89 0.27834908834885397 1103 90-91 0.30830217786095204 1103 92-93 0.343977535163539 1103 94-95 0.3072785652587342 1103 96-97 0.3903859534232694 1103 98-99 0.40406379666507775 1103 100 0.4085966576476636 1104 1 0.06701678552005319 1104 2 0.07105405812281163 1104 3 0.10017276708601486 1104 4 0.07736561681665677 1104 5 -0.008349678928105675 1104 6 0.046796715164376224 1104 7 0.060354465453436035 1104 8 0.04153653415297498 1104 9 0.09754564088746065 1104 10-11 0.11085877985308912 1104 12-13 0.09697270979420836 1104 14-15 0.16894147124808967 1104 16-17 0.12621640841877735 1104 18-19 0.11605137832980006 1104 20-21 0.15471295580061906 1104 22-23 0.14833514774305456 1104 24-25 0.12519517231898902 1104 26-27 0.08949956160612516 1104 28-29 0.11373334996143569 1104 30-31 0.06867172954230938 1104 32-33 0.06409364665343276 1104 34-35 0.12819916286094468 1104 36-37 0.03193048087658923 1104 38-39 0.09924577242152566 1104 40-41 0.07715961133573046 1104 42-43 0.08485503984916676 1104 44-45 0.15237919317314663 1104 46-47 0.24105778981534343 1104 48-49 0.1999770937234615 1104 50-51 0.16685306009233614 1104 52-53 0.1591309135006611 1104 54-55 0.1786287671123432 1104 56-57 0.19826138722788755 1104 58-59 0.2204327728323463 1104 60-61 0.2352967827142649 1104 62-63 0.21064415036906325 1104 64-65 0.1696019908480011 1104 66-67 0.1310394095820513 1104 68-69 0.13275125413571232 1104 70-71 0.14563690401702445 1104 72-73 0.11562126948464169 1104 74-75 0.18465787806151468 1104 76-77 0.16644411348641341 1104 78-79 0.1559055089329675 1104 80-81 0.18956689444955543 1104 82-83 0.19617987347142218 1104 84-85 0.190254706691654 1104 86-87 0.16197444886264378 1104 88-89 0.13319513585378928 1104 90-91 0.16987693196521292 1104 92-93 0.1341780533495438 1104 94-95 0.17408876452488897 1104 96-97 0.2470072407262336 1104 98-99 0.27382507520381694 1104 100 0.2214684634723909 1105 1 -0.002918802555186062 1105 2 0.017847724318066582 1105 3 -0.01587636487349897 1105 4 0.026998097628656126 1105 5 -0.006230045181595756 1105 6 -0.025977480375345863 1105 7 -0.08227561632820368 1105 8 -0.0677027260897205 1105 9 0.014436101049284389 1105 10-11 -0.03826301366292029 1105 12-13 0.008716601443630623 1105 14-15 0.01567267067834166 1105 16-17 0.03557136837935815 1105 18-19 -0.02830560240110458 1105 20-21 -0.002750470949408168 1105 22-23 0.035951083096634306 1105 24-25 -0.036142157119613216 1105 26-27 -0.014096327208619641 1105 28-29 0.0010358898346112255 1105 30-31 0.009797424978351899 1105 32-33 -0.030327494312231806 1105 34-35 -0.026365841208559004 1105 36-37 -0.06270640917133363 1105 38-39 -0.039432620996670664 1105 40-41 0.05523523724110646 1105 42-43 0.08738605209662609 1105 44-45 0.10452821146820668 1105 46-47 0.092938328419514 1105 48-49 0.018394825377171742 1105 50-51 0.05895922790596586 1105 52-53 0.09713396982515121 1105 54-55 0.13943509279671673 1105 56-57 0.05244380178223906 1105 58-59 0.006876578534296129 1105 60-61 -0.05347637250679327 1105 62-63 -0.004468974446183438 1105 64-65 -0.07639774358279539 1105 66-67 -0.08690483932216608 1105 68-69 -0.07753325499732 1105 70-71 -0.0726188242091581 1105 72-73 -0.1065230951348326 1105 74-75 -0.11785545374026896 1105 76-77 -0.09663700816852128 1105 78-79 -0.08155248317743258 1105 80-81 -0.08768411526236264 1105 82-83 -0.02983466509587629 1105 84-85 -0.04882085976102957 1105 86-87 -0.06714615663513257 1105 88-89 -0.14582417736602338 1105 90-91 -0.1478809938357486 1105 92-93 -0.07372202965636632 1105 94-95 -0.1713659626586903 1105 96-97 -0.08417303829543243 1105 98-99 -0.14151938172270917 1105 100 -0.1543014438083219 1106 1 -0.004225776490372368 1106 2 0.003299031643990702 1106 3 -0.009019796078916897 1106 4 0.018831084573989187 1106 5 -0.02716527592993856 1106 6 -0.006668195996994086 1106 7 -0.027983573019739083 1106 8 0.04011486490789906 1106 9 0.06951015728699161 1106 10-11 0.01879101166113628 1106 12-13 0.015707521388094392 1106 14-15 0.027729347826380035 1106 16-17 0.03156790241714447 1106 18-19 0.07377727669148726 1106 20-21 0.06253920549832515 1106 22-23 0.05880649419082573 1106 24-25 0.04210965381986398 1106 26-27 0.027984212884980764 1106 28-29 -0.011168871034712424 1106 30-31 0.09133027938701588 1106 32-33 0.07686000962286954 1106 34-35 0.06121006347292024 1106 36-37 0.01199399520083233 1106 38-39 0.065096341393037 1106 40-41 0.0714399193836428 1106 42-43 0.021139334404288945 1106 44-45 0.0032768965868967825 1106 46-47 0.03277970085964199 1106 48-49 0.029091903591648816 1106 50-51 -0.07294789608606322 1106 52-53 -0.11163752920945313 1106 54-55 -0.09706326112902275 1106 56-57 -0.07025687248205514 1106 58-59 -0.08490188960678324 1106 60-61 -0.05758652617178939 1106 62-63 -0.07036055396059737 1106 64-65 -0.07856577949095112 1106 66-67 -0.12737723085492547 1106 68-69 -0.07112004499735747 1106 70-71 -0.08204001268438077 1106 72-73 -0.04357596236188499 1106 74-75 -0.11019250537801639 1106 76-77 -0.15327575954165695 1106 78-79 -0.10684024499732914 1106 80-81 -0.07882350819942019 1106 82-83 -0.12240989504704203 1106 84-85 -0.10917761808492799 1106 86-87 -0.15302646056817082 1106 88-89 -0.18937060748741175 1106 90-91 -0.1981448575110747 1106 92-93 -0.21727563839270658 1106 94-95 -0.18716652546607548 1106 96-97 -0.23377237441236787 1106 98-99 -0.1656660263498324 1106 100 -0.10691718137481843 1107 1 -0.019039770057432293 1107 2 0.03116225565275954 1107 3 0.031005853486043122 1107 4 0.051459083408516904 1107 5 -0.05313945571032974 1107 6 -0.07665001471324473 1107 7 -0.041202744671203106 1107 8 0.008442303806823759 1107 9 0.04465992957148046 1107 10-11 0.029136450344893206 1107 12-13 0.06764346710735936 1107 14-15 0.07461478633320695 1107 16-17 0.07723500085719337 1107 18-19 0.09642497500831126 1107 20-21 0.07866499966313256 1107 22-23 0.05635440377125889 1107 24-25 0.10449063433070194 1107 26-27 0.058440146173282415 1107 28-29 -0.00409447959118836 1107 30-31 0.004176550654285904 1107 32-33 0.03218837285358234 1107 34-35 0.08726179409084267 1107 36-37 0.07130854282475951 1107 38-39 0.037903464826285926 1107 40-41 0.02128143600691601 1107 42-43 -0.0849707583913144 1107 44-45 -0.07751844825106957 1107 46-47 -0.0525793003026962 1107 48-49 -0.11262979063802447 1107 50-51 -0.08275490222992232 1107 52-53 -0.1302736304299188 1107 54-55 -0.06074713851680258 1107 56-57 -0.10220139659387684 1107 58-59 -0.07087531884916842 1107 60-61 -0.058432522336907766 1107 62-63 0.008926865852401988 1107 64-65 -0.07432359885557105 1107 66-67 -0.037929723853949326 1107 68-69 0.030908605007368806 1107 70-71 0.01073490325108395 1107 72-73 0.026832921040181645 1107 74-75 -0.013377740155149809 1107 76-77 -0.02077776359227812 1107 78-79 -0.08455682871956327 1107 80-81 -0.0336317742768415 1107 82-83 0.03481454692224162 1107 84-85 -0.02144693109723761 1107 86-87 -0.03382811220838278 1107 88-89 -4.644723957127894E-4 1107 90-91 -0.0238033689972994 1107 92-93 0.020862793348150888 1107 94-95 0.022978051554396473 1107 96-97 0.01094560736525807 1107 98-99 0.07509878899926292 1107 100 0.08161810803748892 1108 1 0.054981576285513256 1108 2 0.02524709345677678 1108 3 0.016294541480903035 1108 4 0.02481596408178177 1108 5 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-0.10764365291570499 2214 18-19 -0.09948329153414193 2214 20-21 -0.1566605101846008 2214 22-23 -0.17020307862767226 2214 24-25 -0.1355050044889765 2214 26-27 -0.153329915858734 2214 28-29 -0.13740147839763495 2214 30-31 -0.09277782832975134 2214 32-33 -0.09879259968962373 2214 34-35 -0.15611997523671306 2214 36-37 -0.1592847438751548 2214 38-39 -0.16575071716889056 2214 40-41 -0.1201300895600923 2214 42-43 -0.10789397459816286 2214 44-45 -0.11730349965223752 2214 46-47 -0.07571017364110588 2214 48-49 -0.1951818092568942 2214 50-51 -0.07012301870362592 2214 52-53 -0.13888404395937215 2214 54-55 -0.05460076649745815 2214 56-57 -0.10225353286820393 2214 58-59 -0.2027225019744705 2214 60-61 -0.20015876833360835 2214 62-63 -0.23221450247996955 2214 64-65 -0.06295503687676529 2214 66-67 -0.17479575278361636 2214 68-69 -0.1410039729893171 2214 70-71 -0.08290723666009825 2214 72-73 -0.14211137775673421 2214 74-75 -0.1740089036680743 2214 76-77 -0.0649159401952204 2214 78-79 -0.22723402260066905 2214 80-81 -0.1930580951290608 2214 82-83 -0.1664891263256436 2214 84-85 -0.16991619866630003 2214 86-87 -0.15040856374403688 2214 88-89 -0.23817975176178408 2214 90-91 -0.11301411586909893 2214 92-93 -0.04444480369350856 2214 94-95 -0.14839115483383125 2214 96-97 -0.2537161341359848 2214 98-99 -0.32759418276456387 2214 100 -0.5091489256507913 2215 1 -0.06530464681349457 2215 2 -0.1039194314872276 2215 3 -0.10989819648765575 2215 4 -0.06771047326951418 2215 5 -0.054151907907645125 2215 6 -0.11188271628432034 2215 7 -0.08508346512094533 2215 8 -0.10696413650722292 2215 9 -0.156584712982351 2215 10-11 -0.17161864242406466 2215 12-13 -0.13982195582374857 2215 14-15 -0.21169699023634792 2215 16-17 -0.18899274275715783 2215 18-19 -0.16702586346602288 2215 20-21 -0.1761604123180831 2215 22-23 -0.21564249925793177 2215 24-25 -0.18367000742497197 2215 26-27 -0.1588667136665265 2215 28-29 -0.12285362167420999 2215 30-31 -0.1658620913949278 2215 32-33 -0.21417476841148897 2215 34-35 -0.18217693354557696 2215 36-37 -0.14264254383600417 2215 38-39 -0.2322852640469435 2215 40-41 -0.22636907957770802 2215 42-43 -0.24559460094383212 2215 44-45 -0.2879239733261443 2215 46-47 -0.3097677191490362 2215 48-49 -0.19509372939781855 2215 50-51 -0.20561186192146863 2215 52-53 -0.19870984157142857 2215 54-55 -0.21576244197211736 2215 56-57 -0.2828612838958051 2215 58-59 -0.3071754281831218 2215 60-61 -0.2471077798818584 2215 62-63 -0.23802532651598085 2215 64-65 -0.20291393294252913 2215 66-67 -0.16612722665325919 2215 68-69 -0.08678347974210965 2215 70-71 -0.08243013742345795 2215 72-73 -0.0509560636052413 2215 74-75 -0.10809091580352259 2215 76-77 -0.1508549693594361 2215 78-79 -0.12482161312718887 2215 80-81 -0.0820285394430158 2215 82-83 -0.08448922419215421 2215 84-85 -0.0709756036379261 2215 86-87 -0.1371892448949481 2215 88-89 -0.1524658155707428 2215 90-91 -0.055703487566098886 2215 92-93 -0.11283636760033744 2215 94-95 -0.07604089059424979 2215 96-97 -0.07071065361142814 2215 98-99 -0.13530117043337953 2215 100 -0.09727771797805929 2216 1 -0.0541219225892533 2216 2 -0.07852194918223887 2216 3 -0.1353450717622593 2216 4 -0.07099407820512482 2216 5 -0.05379007074429154 2216 6 -0.05078488844114304 2216 7 -0.07944876900463527 2216 8 -0.07124401960816584 2216 9 -0.12841626382238758 2216 10-11 -0.12657554936119197 2216 12-13 -0.1272412050506091 2216 14-15 -0.20250656722843985 2216 16-17 -0.20424847361720566 2216 18-19 -0.07088737323265093 2216 20-21 -0.09408977682828379 2216 22-23 -0.13438277302396529 2216 24-25 -0.2214573299433482 2216 26-27 -0.23518625712524965 2216 28-29 -0.22493344128002946 2216 30-31 -0.24075313631489337 2216 32-33 -0.20956807676251543 2216 34-35 -0.224312254692002 2216 36-37 -0.2610316810676707 2216 38-39 -0.2244894660634742 2216 40-41 -0.23782179980515394 2216 42-43 -0.21549582209110696 2216 44-45 -0.10897430738419445 2216 46-47 -0.13081606898448683 2216 48-49 -0.1487512185625448 2216 50-51 -0.0426186408762419 2216 52-53 -0.022706320993620466 2216 54-55 -0.08898231084334896 2216 56-57 -0.04838619581049386 2216 58-59 -0.04569301548183091 2216 60-61 -0.07424999620180728 2216 62-63 -0.09704613392450767 2216 64-65 -0.07991735997841687 2216 66-67 -0.10655735650644971 2216 68-69 -0.06599120091915012 2216 70-71 -0.06775202793405555 2216 72-73 0.00951032082937786 2216 74-75 -0.030063984233692054 2216 76-77 -0.04362421419266127 2216 78-79 -0.03805389756266209 2216 80-81 -0.03272698579671385 2216 82-83 -0.06074534339290949 2216 84-85 0.005877837429252963 2216 86-87 -0.030885263410574737 2216 88-89 -0.0130901582970111 2216 90-91 -0.0061761577344512375 2216 92-93 -0.03241189278153911 2216 94-95 -0.10237708734976536 2216 96-97 -0.16017733709674076 2216 98-99 -0.10252386823911053 2216 100 -0.11312527865983668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15.0 3 7.0 4 11.0 5 38.0 6 52.0 7 147.0 8 348.0 9 604.0 10 1124.0 11 1980.0 12 2935.0 13 4682.0 14 5229.0 15 7216.0 16 9695.0 17 15246.0 18 18190.0 19 9694.0 20 5948.0 21 5395.0 22 5606.0 23 6083.0 24 7087.0 25 8400.0 26 10537.0 27 13579.0 28 17376.0 29 22481.0 30 28903.0 31 36742.0 32 47387.0 33 62504.0 34 88561.0 35 137230.0 36 262969.0 37 618362.0 38 1077440.0 39 470198.0 40 1707.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.44831093005031 19.553137423419724 13.371512967715477 23.62703867881449 2 15.923164198987338 20.076973930402044 39.804706294084774 24.19515557652584 3 18.277336315472816 25.80263425272304 29.71124690707067 26.208782524733476 4 12.183197712120442 16.724358393291226 38.671420132901815 32.42102376168652 5 12.484953005545798 38.35349342715394 35.596903972938 13.56464959436226 6 30.876673139192963 38.19721327195501 17.47797924364413 13.448134345207896 7 27.54331712140748 32.51713641626674 22.47051287883492 17.469033583490866 8 25.852541753826742 35.67792276787197 19.913238370355614 18.556297107945678 9 25.923173699398642 15.765067139754485 20.74749734618586 37.56426181466101 10-11 24.32220465920431 26.48273256135375 28.564550620921214 20.630512158520727 12-13 25.253543836255044 24.051966525307233 28.706850066473912 21.987639571963815 14-15 22.347500054786224 25.585930366377063 26.336734063683508 25.729835515153205 16-17 21.32651970244127 29.072141123907098 26.910726405083096 22.69061276856853 18-19 21.293493650106385 27.56522553345216 29.73675367831633 21.40452713812513 20-21 22.498280045741488 26.799327823281672 29.45617569830807 21.24621643266877 22-23 21.98152406845033 26.684097563437554 29.551446325034814 21.782932043077302 24-25 21.70348110297046 26.951939326032594 29.492777094425133 21.851802476571812 26-27 21.397643952459347 26.957296555314787 29.59355556780427 22.0515039244216 28-29 21.670042142811365 26.833740283261058 29.786443436948662 21.70977413697891 30-31 21.846996619249353 27.303115618483385 29.264286478259628 21.585601284007634 32-33 21.458319390891166 26.990735797266623 29.72846405407304 21.82248075776917 34-35 21.81475324623563 26.74469699863325 29.635777261487416 21.804772493643707 36-37 22.0689466202572 27.024956158996705 29.027410590923587 21.87868662982251 38-39 21.7694802984223 27.280355614482175 29.256940032236194 21.693224054859325 40-41 21.716029759515493 27.006466362278044 29.3476558627139 21.929848015492563 42-43 21.682802162765363 26.988402370008234 29.205984137949358 22.12281132927704 44-45 21.6752363669696 27.19472227794391 29.09500710064045 22.035034254446035 46-47 22.008511599254113 27.276321448200626 28.675886735620693 22.039280216924567 48-49 22.07965966077988 27.334564564679248 28.375892062974444 22.20988371156643 50-51 22.100252088827315 27.313873419629854 28.148275317593235 22.437599173949593 52-53 22.14577877896957 27.399417968721462 28.176151460955623 22.278651791353344 54-55 22.14686667275429 27.801283381619253 28.032823356549315 22.019026589077146 56-57 21.994980448253244 27.404952843541036 28.10859325543183 22.491473452773896 58-59 21.947498624933946 26.967896752420206 28.437959825326352 22.646644797319496 60-61 22.077622595305787 27.394176852678108 28.208404756469584 22.319795795546515 62-63 21.851728442470115 27.33356211108405 28.28252491610514 22.532184530340697 64-65 21.934609835586556 27.23454027158186 28.202376465783306 22.628473427048277 66-67 21.813186129038954 27.258173366260607 28.195920061427355 22.732720443273084 68-69 22.186387556940982 27.406811700215595 28.099500244216586 22.30730049862684 70-71 22.203408514745693 27.20385178701813 28.113871500182785 22.478868198053387 72-73 21.990768295438453 27.313334239802185 28.055659179665614 22.64023828509375 74-75 22.034164218151343 27.436929908765478 27.89150871819766 22.637397154885512 76-77 22.162547417258764 27.34278903215085 27.815347079641406 22.679316470948983 78-79 22.191243102746416 27.523735716567288 27.85353218408437 22.431488996601924 80-81 22.168406026978527 27.28719060368567 27.957727409995346 22.586675959340457 82-83 22.035606900640385 27.39935348623855 28.007699327769792 22.55734028535127 84-85 21.937755337856142 27.357342839383524 27.79765952473291 22.907242298027423 86-87 22.14193538104623 27.34203572299579 27.82286026686244 22.693168629095545 88-89 21.896349496091503 27.358865361261014 27.909317222871078 22.835467919776406 90-91 21.858624314591133 27.25251070323597 27.987775035146196 22.9010899470267 92-93 21.907417257859834 27.449110238515978 27.760329195479237 22.883143308144952 94-95 21.796884027269574 27.159986957566932 27.978160565366895 23.064968449796595 96-97 21.83644629559041 27.220716616617548 27.856585034140096 23.086252053651947 98-99 21.53980399162203 27.40659121003763 27.81478151268317 23.238823285657176 100 21.627362280805443 27.177933584530773 27.840879660312357 23.35382447435143 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 49.0 1 41.0 2 127.0 3 244.5 4 183.0 5 141.5 6 313.5 7 733.5 8 1329.5 9 1600.5 10 1357.0 11 1088.0 12 1117.5 13 1291.5 14 1481.0 15 1703.5 16 1879.5 17 2133.0 18 2494.0 19 2885.5 20 3356.0 21 3940.0 22 4920.5 23 6628.5 24 8840.0 25 11276.5 26 14979.0 27 20213.5 28 26515.5 29 33608.0 30 40032.0 31 46390.5 32 53961.5 33 61809.0 34 69400.5 35 77451.0 36 85243.5 37 92646.5 38 99197.5 39 102698.5 40 106291.5 41 110594.0 42 113642.0 43 116707.0 44 115547.5 45 114179.5 46 115807.5 47 114104.0 48 109850.5 49 106309.5 50 104483.5 51 100062.5 52 90152.5 53 82790.0 54 79597.5 55 76468.5 56 70669.5 57 62829.0 58 56228.0 59 50292.5 60 44062.0 61 37292.0 62 31653.0 63 26222.5 64 21495.5 65 17531.0 66 13546.0 67 10284.5 68 7681.0 69 5819.5 70 4177.0 71 2902.0 72 2212.0 73 1551.5 74 1095.0 75 810.5 76 542.0 77 350.5 78 232.0 79 155.0 80 94.5 81 52.5 82 31.5 83 13.5 84 3.0 85 2.0 86 4.0 87 3.5 88 1.0 89 1.0 90 0.5 91 0.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09210720295593065 2 0.001294946256409984 3 0.0 4 3.6524125180794416E-4 5 0.037852275187368764 6 1.3281500065743425E-4 7 0.00461532127284584 8 2.656300013148685E-4 9 1.6601875082179282E-4 10-11 4.4825062721884064E-4 12-13 0.0 14-15 6.640750032871713E-5 16-17 0.0 18-19 1.3281500065743425E-4 20-21 0.0 22-23 1.992225009861514E-4 24-25 1.4941687573961354E-4 26-27 0.0017930025088753626 28-29 0.009330253796184757 30-31 0.03765305268638261 32-33 0.027144065759363126 34-35 0.03031502390005937 36-37 0.02749270513608889 38-39 0.02679542638263736 40-41 0.032323850785003064 42-43 0.024836405122940206 44-45 0.023641070117023297 46-47 0.01736556133595953 48-49 0.011936748184086904 50-51 0.008948410669294634 52-53 0.006175897530570692 54-55 0.004416098771859689 56-57 0.0028057168888882987 58-59 0.0011455293806703704 60-61 6.806768783693505E-4 62-63 0.001709993133464466 64-65 0.003087948765285346 66-67 6.806768783693505E-4 68-69 0.0012285387560812668 70-71 4.980562524653784E-4 72-73 7.138806285337091E-4 74-75 6.308712531228127E-4 76-77 4.814543773831992E-4 78-79 6.640750032871713E-5 80-81 5.478618777119163E-4 82-83 5.478618777119163E-4 84-85 3.984450019723028E-4 86-87 2.1582437606833065E-4 88-89 1.660187508217928E-5 90-91 6.640750032871713E-5 92-93 6.640750032871713E-5 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3011708.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.719619555160236 #Duplication Level Percentage of deduplicated Percentage of total 1 71.52779060944442 36.99390117941628 2 15.556269664141936 16.091286974538026 3 5.548955622936058 8.609696212401603 4 2.5402260318625935 5.255180958081906 5 1.3974792502288627 3.6138547579033666 6 0.8068435540356536 2.5037784993154424 7 0.5207426780468104 1.8852829236301427 8 0.37283604240252344 1.542635061560808 9 0.2567787402624261 1.1952448880603384 >10 1.3128300696460469 12.227423573213729 >50 0.09919072101482024 3.546646326709436 >100 0.05832011467810983 5.491754601543686 >500 0.0014152529109967278 0.455447603351174 >1k 1.9298903324145472E-4 0.1838226318701777 >5k 1.2865935549430315E-4 0.40404380840388016 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6629 0.2201076598395329 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5515 0.18311868215643748 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 1.660187508217928E-5 0.0 24-25 0.0 0.0 0.0 1.4941687573961354E-4 0.0 26-27 0.0 0.0 0.0 3.3203750164358564E-4 0.0 28-29 0.0 0.0 0.0 0.0011953350059169083 0.0 30-31 0.0 0.0 0.0 0.00508017377514686 0.0 32-33 0.0 0.0 0.0 0.014642853822482125 0.0 34-35 0.0 0.0 0.0 0.02702785263378787 0.0 36-37 0.0 0.0 0.0 0.050287079623921045 0.0 38-39 0.0 0.0 0.0 0.09139332232739694 0.0 40-41 0.0 0.0 0.0 0.1406344838211407 0.0 42-43 0.0 0.0 0.0 0.18876331968437843 0.0 44-45 0.0 0.0 0.0 0.2425035893253928 0.0 46-47 0.0 0.0 0.0 0.31141797279151895 0.0 48-49 0.0 0.0 0.0 0.38896533130037836 0.0 50-51 0.0 0.0 0.0 0.47682245423527114 0.0 52-53 0.0 0.0 0.0 0.5608611459012627 0.0 54-55 0.0 0.0 0.0 0.651739810101112 0.0 56-57 0.0 0.0 0.0 0.7588385062562506 0.0 58-59 0.0 0.0 0.0 0.8824560681181575 0.0 60-61 0.0 0.0 0.0 1.012498555636868 0.0 62-63 0.0 0.0 0.0 1.146425881924808 0.0 64-65 0.0 0.0 0.0 1.284387463857718 0.0 66-67 0.0 0.0 0.0 1.443499834645324 0.0 68-69 0.0 0.0 0.0 1.6285775380614589 0.0 70-71 0.0 0.0 0.0 1.830107699684033 0.0 72-73 0.0 0.0 0.0 2.041848014482148 0.0 74-75 0.0 0.0 0.0 2.2504007692644836 0.0 76-77 0.0 0.0 0.0 2.4738288041204526 0.0 78-79 0.0 0.0 0.0 2.729663699136835 0.0 80-81 0.0 0.0 0.0 3.0056864742531477 0.0 82-83 0.0 0.0 0.0 3.302212565095952 0.0 84-85 0.0 0.0 0.0 3.598805063439085 0.0 86-87 0.0 0.0 0.0 3.9093431368512483 0.0 88 0.0 0.0 0.0 4.166472978124041 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4520 0.0 57.260635 1 GTATCAA 9065 0.0 43.65762 1 ATCAACG 10745 0.0 36.403625 3 TCAACGC 10800 0.0 36.21824 4 CAACGCA 10965 0.0 35.673233 5 AACGCAG 11480 0.0 34.10083 6 TATCAAC 11560 0.0 34.041023 2 ACGCAGA 13425 0.0 28.845282 7 CGCAGAG 13750 0.0 28.127438 8 GTACATG 11570 0.0 27.413116 1 TACATGG 11315 0.0 27.049633 2 ACATGGG 11620 0.0 25.610931 3 GCAGAGT 15090 0.0 25.162579 9 GTGGTAT 1950 0.0 23.890898 1 CCGATCG 105 0.0023383943 22.377611 9 GAGTACT 9545 0.0 21.613329 12-13 CAGAGTA 14910 0.0 20.785965 10-11 TGGTATC 1975 0.0 20.472284 2 CATGGGG 6860 0.0 20.423018 4 AGTACTT 9685 0.0 20.403254 12-13 >>END_MODULE