FastQCFastQC Report
Wed 25 May 2016
SRR1033163_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033163_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2932462
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24900.034.997851
TCAACGC36600.033.630954
GTATCAA38850.032.9797131
ATCAACG37400.032.9177553
CAACGCA39600.031.201265
AACGCAG42800.028.8581166
GTACATG79700.027.3056561
TACATGG78900.026.5759072
TATCAAC48100.026.3905852
ACGCAGA49850.024.1174167
ACATGGG84200.024.1086083
CGCAGAG50750.023.2274258
TATACCG1656.886135E-519.9308415
TAGACCG1200.005078551419.5749345
GTATTAG15250.018.8420851
CATGGGG53850.018.6701184
GCAGAGT63650.018.150989
GTATAAC8100.017.4463771
GAGTACA57500.016.8759561
AGAGTAC52250.016.53873610-11
Produced by FastQC (version 0.11.5)