##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033163_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2932462
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.714642849591915	33.0	31.0	34.0	30.0	34.0
2	31.861706306850696	34.0	31.0	34.0	30.0	34.0
3	31.94503492287368	34.0	31.0	34.0	30.0	34.0
4	35.29844240095865	37.0	35.0	37.0	33.0	37.0
5	35.39732893384467	37.0	35.0	37.0	35.0	37.0
6	35.437631246372504	37.0	37.0	37.0	35.0	37.0
7	35.40658190967181	37.0	37.0	37.0	35.0	37.0
8	35.41075689983366	37.0	37.0	37.0	35.0	37.0
9	37.071185918180696	39.0	38.0	39.0	35.0	39.0
10-11	37.06838997402183	39.0	38.0	39.0	34.5	39.0
12-13	37.03107678803681	39.0	38.0	39.0	35.0	39.0
14-15	38.50000784323889	41.0	39.0	41.0	35.0	41.0
16-17	38.512474159937966	41.0	39.0	41.0	35.0	41.0
18-19	38.50365017517703	41.0	39.0	41.0	34.0	41.0
20-21	38.538383617588224	41.0	39.0	41.0	34.0	41.0
22-23	38.50636973300932	41.0	39.0	41.0	34.0	41.0
24-25	38.50201707643612	41.0	39.0	41.0	34.0	41.0
26-27	38.46946712352965	41.0	39.0	41.0	34.0	41.0
28-29	38.44050050776447	40.5	39.0	41.0	34.0	41.0
30-31	38.43681691356955	41.0	39.0	41.0	34.0	41.0
32-33	38.50467337684171	41.0	39.0	41.0	34.0	41.0
34-35	38.48080691241694	41.0	39.0	41.0	34.0	41.0
36-37	38.40487924481204	41.0	39.0	41.0	34.0	41.0
38-39	38.33272434561812	41.0	39.0	41.0	34.0	41.0
40-41	38.27664825665261	41.0	39.0	41.0	33.5	41.0
42-43	38.171208697674516	40.5	38.5	41.0	33.0	41.0
44-45	38.05421280821371	40.0	38.0	41.0	33.0	41.0
46-47	37.93851599782026	40.0	38.0	41.0	33.0	41.0
48-49	37.83157241253254	40.0	38.0	41.0	33.0	41.0
50-51	37.72047736679964	40.0	38.0	41.0	33.0	41.0
52-53	37.588184944937055	40.0	38.0	41.0	32.0	41.0
54-55	37.44957308909714	40.0	37.0	41.0	32.0	41.0
56-57	37.30934586705642	40.0	37.0	41.0	32.0	41.0
58-59	37.13181176772282	40.0	36.5	41.0	31.0	41.0
60-61	36.92734381553794	40.0	36.0	41.0	31.0	41.0
62-63	36.68522013243479	39.0	35.5	41.0	31.0	41.0
64-65	36.430462185017234	39.0	35.0	41.0	31.0	41.0
66-67	36.150901870169164	39.0	35.0	41.0	31.0	41.0
68-69	35.85521875475283	38.0	35.0	40.0	30.0	41.0
70-71	35.51359983522378	37.0	35.0	40.0	30.0	41.0
72-73	35.14928667447353	37.0	35.0	39.5	30.0	41.0
74-75	34.776460871445224	36.0	34.5	39.0	29.0	41.0
76-77	34.43490793742596	36.0	34.0	39.0	29.0	40.5
78-79	34.051763671617906	35.5	34.0	37.5	29.0	39.5
80-81	33.67653545041675	35.0	34.0	37.0	29.0	39.0
82-83	33.432368433077734	35.0	34.0	37.0	29.0	39.0
84-85	33.14484671924137	35.0	34.0	36.0	29.0	38.0
86-87	32.869598310225335	35.0	34.0	36.0	29.0	37.0
88-89	32.48980685853729	35.0	34.0	36.0	27.5	37.0
90-91	32.285334302712194	35.0	34.0	35.0	27.0	36.5
92-93	32.09629775253695	35.0	34.0	35.0	27.0	36.0
94-95	31.921930787167916	35.0	34.0	35.0	27.0	36.0
96-97	31.74419787877899	35.0	34.0	35.0	26.0	36.0
98-99	31.55693901574854	35.0	33.0	35.0	25.5	35.5
100	31.398638413728804	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.11952649113878522
1101	2	0.17041560298072156
1101	3	0.15445006643619408
1101	4	0.15403224436438023
1101	5	0.11393985179153532
1101	6	0.12047869945823209
1101	7	0.14526985367882617
1101	8	0.08623703238667701
1101	9	0.1833466527960539
1101	10-11	0.16035715115668836
1101	12-13	0.19545668318705367
1101	14-15	0.2131224755468608
1101	16-17	0.14023610368185757
1101	18-19	0.14186137855482173
1101	20-21	0.1597056532681833
1101	22-23	0.2257061161419287
1101	24-25	0.24764414257565903
1101	26-27	0.17717247400393887
1101	28-29	0.2866972101669276
1101	30-31	0.2752854780702023
1101	32-33	0.26833548262811036
1101	34-35	0.28332559851267547
1101	36-37	0.23599341488570502
1101	38-39	0.2360126651574177
1101	40-41	0.25228380369348713
1101	42-43	0.2088431884276858
1101	44-45	0.20911574531113786
1101	46-47	0.16870553890529294
1101	48-49	0.1856780532594584
1101	50-51	0.13774494101118506
1101	52-53	0.1739978910872324
1101	54-55	0.1329049906451516
1101	56-57	0.17665187338212007
1101	58-59	0.20339671691194638
1101	60-61	0.14881953925331715
1101	62-63	0.15748176516696333
1101	64-65	0.13168157828377502
1101	66-67	0.1377499119728185
1101	68-69	0.16565932864101995
1101	70-71	0.2122797082353287
1101	72-73	0.3007820815391824
1101	74-75	0.263117990704707
1101	76-77	0.22387477570775616
1101	78-79	0.3041698721492665
1101	80-81	0.23359601450178502
1101	82-83	0.20865616126076247
1101	84-85	0.20144602038789117
1101	86-87	0.17384020926395038
1101	88-89	0.38989433151429154
1101	90-91	0.38979017172003694
1101	92-93	0.4180347078217963
1101	94-95	0.41899336806347165
1101	96-97	0.3975477004828214
1101	98-99	0.36820869111469534
1101	100	0.421274943123521
1102	1	0.20890579323397063
1102	2	0.2173084217835175
1102	3	0.13964867455556274
1102	4	0.20105493095433502
1102	5	0.134807753358821
1102	6	0.13548636956840454
1102	7	0.08072676205479468
1102	8	0.15905258818634138
1102	9	0.21075259139166747
1102	10-11	0.23612421760878277
1102	12-13	0.15432143616467897
1102	14-15	0.2075336959319003
1102	16-17	0.20384444089414444
1102	18-19	0.2665438332369945
1102	20-21	0.19846894036989937
1102	22-23	0.2539564650379944
1102	24-25	0.2527804225473602
1102	26-27	0.25189008048608486
1102	28-29	0.25999505669292233
1102	30-31	0.21204612838239711
1102	32-33	0.29074285983757875
1102	34-35	0.2760914473718614
1102	36-37	0.2988692306570684
1102	38-39	0.2430929766075849
1102	40-41	0.2486876286264419
1102	42-43	0.25314233716741086
1102	44-45	0.20618532993378125
1102	46-47	0.14690875604262033
1102	48-49	0.15851468036230187
1102	50-51	0.14947058784954947
1102	52-53	0.07294801760195213
1102	54-55	0.21534484618806005
1102	56-57	0.06238883651710836
1102	58-59	0.1310537671895986
1102	60-61	0.11131897216797881
1102	62-63	0.2289257984732913
1102	64-65	0.22977925192441262
1102	66-67	0.15085631048738435
1102	68-69	0.0860542106156501
1102	70-71	0.1623848879583818
1102	72-73	0.152158347472934
1102	74-75	0.14305480196569675
1102	76-77	0.0792221073142727
1102	78-79	0.16991351865095083
1102	80-81	0.2091954034819281
1102	82-83	0.16095710741382163
1102	84-85	0.19818823870348723
1102	86-87	0.18572350133264592
1102	88-89	0.3159462830639512
1102	90-91	0.3485373724759171
1102	92-93	0.41342862642555644
1102	94-95	0.32448491763787857
1102	96-97	0.2770273375793657
1102	98-99	0.3314759740630393
1102	100	0.31110552828787164
1103	1	-0.022231238638990902
1103	2	0.025486881397810635
1103	3	0.0196422868114432
1103	4	0.021237250054362278
1103	5	0.045443749907292386
1103	6	-0.03962773083996041
1103	7	0.03364801432103093
1103	8	-0.025859184452912132
1103	9	0.021494200432201183
1103	10-11	-0.042630360162668524
1103	12-13	0.009612192588988933
1103	14-15	-0.019574762863797446
1103	16-17	-0.09145788325166393
1103	18-19	-0.0026935425810989955
1103	20-21	-0.021853882261503088
1103	22-23	-0.031501218170156164
1103	24-25	-0.07453912703616083
1103	26-27	-0.01887243633877489
1103	28-29	-0.06552626240307546
1103	30-31	0.004371307500917965
1103	32-33	-0.033731728079118284
1103	34-35	-0.06887357436123409
1103	36-37	-0.06056416570379497
1103	38-39	-0.05187519873867075
1103	40-41	-0.08244175405714316
1103	42-43	-0.10534296229834439
1103	44-45	-0.1913094934437538
1103	46-47	-0.15587299916453645
1103	48-49	-0.12084847765250828
1103	50-51	-0.1864683904066169
1103	52-53	-0.21122406500392543
1103	54-55	-0.17850453456308912
1103	56-57	-0.23573966181282202
1103	58-59	-0.2115232312953239
1103	60-61	-0.19085017690667883
1103	62-63	-0.18174978598516844
1103	64-65	-0.19734611560572546
1103	66-67	-0.12336176119223552
1103	68-69	-0.17610431011163996
1103	70-71	-0.22617313248508708
1103	72-73	-0.3029375559164649
1103	74-75	-0.22585486647548692
1103	76-77	-0.19583402029031305
1103	78-79	-0.20416874636796223
1103	80-81	-0.16080595190075542
1103	82-83	-0.1628062400861694
1103	84-85	-0.09384640541461664
1103	86-87	-0.0895681407872786
1103	88-89	-0.10878770631038748
1103	90-91	-0.09963735648109662
1103	92-93	-0.12788303509915266
1103	94-95	-0.10081672210677439
1103	96-97	-0.10612324275255602
1103	98-99	-0.06685220836878614
1103	100	-0.06884044427971858
1104	1	0.17846044624534585
1104	2	0.1318613819313157
1104	3	0.14069451969314528
1104	4	0.16995248522919582
1104	5	0.07753007731738393
1104	6	0.07742020921364201
1104	7	0.08711675966570454
1104	8	0.07019698389669315
1104	9	0.07278104915450712
1104	10-11	0.11809381098836269
1104	12-13	0.10822409181059811
1104	14-15	0.1016925619952076
1104	16-17	0.1401467541516439
1104	18-19	0.14293606047310448
1104	20-21	0.035060108263529344
1104	22-23	0.025431414786005746
1104	24-25	-0.006052495377083744
1104	26-27	0.026856771366347232
1104	28-29	0.16939157432020835
1104	30-31	0.08316371163953562
1104	32-33	0.07553524983917725
1104	34-35	-0.005478607423384574
1104	36-37	0.09052251625040952
1104	38-39	0.027944538526952556
1104	40-41	-0.0016755895627511563
1104	42-43	0.06408378857653219
1104	44-45	0.05287425108848254
1104	46-47	0.02939458026785502
1104	48-49	-0.008952297407795129
1104	50-51	-0.018203947239427976
1104	52-53	0.0186830692179214
1104	54-55	-0.06386436868487522
1104	56-57	-0.04732686206604342
1104	58-59	-0.05170738584840251
1104	60-61	0.02817678956707681
1104	62-63	0.05800432541715139
1104	64-65	0.05582446348395109
1104	66-67	-0.05762187492233295
1104	68-69	-0.10818176687852343
1104	70-71	-0.0549550467360973
1104	72-73	-0.04130976191026292
1104	74-75	-0.08951108089610926
1104	76-77	-0.1426530217149633
1104	78-79	-0.11602899346747364
1104	80-81	-0.009468723557162662
1104	82-83	0.014693950703062342
1104	84-85	-0.0032264769936674043
1104	86-87	0.021817146729979697
1104	88-89	0.08943216680280841
1104	90-91	0.04241470208542353
1104	92-93	0.012245558250569388
1104	94-95	0.09843594732322103
1104	96-97	0.10940784547260662
1104	98-99	0.09731748957032593
1104	100	0.09135002391846214
1105	1	0.0777016564371138
1105	2	0.0790585382172786
1105	3	0.03943157294287403
1105	4	0.0429616702964708
1105	5	0.04469292404291991
1105	6	0.09274604248423657
1105	7	0.03229594376499989
1105	8	0.06932176744530949
1105	9	0.021290626791987677
1105	10-11	0.04089017460517397
1105	12-13	0.08241690293593251
1105	14-15	0.06603508756604981
1105	16-17	0.07712431311783519
1105	18-19	0.07808924351837732
1105	20-21	0.074146790991513
1105	22-23	0.11447171507531806
1105	24-25	0.09144016409783973
1105	26-27	0.09184421449744917
1105	28-29	0.12299717032781388
1105	30-31	0.12529294790226686
1105	32-33	0.16050545752495537
1105	34-35	0.16259064626301267
1105	36-37	0.209905535706838
1105	38-39	0.1211346554375865
1105	40-41	0.12337119620147519
1105	42-43	0.11269581090463987
1105	44-45	0.18871254980751928
1105	46-47	0.14177438925286623
1105	48-49	0.10308844922847271
1105	50-51	0.12453094956300959
1105	52-53	0.09808993865874527
1105	54-55	0.10158797436771039
1105	56-57	0.13893963394910003
1105	58-59	0.06774366118473552
1105	60-61	-0.008762453471518938
1105	62-63	0.027554052769083626
1105	64-65	0.06564449962191787
1105	66-67	3.553667610987077E-4
1105	68-69	-0.03480448002263614
1105	70-71	0.025202546001892756
1105	72-73	0.03262747525592147
1105	74-75	0.03349985191523075
1105	76-77	0.04677917657063091
1105	78-79	0.012850863916604283
1105	80-81	0.0680645642452049
1105	82-83	0.08177922448044228
1105	84-85	0.05667261095281617
1105	86-87	0.13176207934772322
1105	88-89	0.08616603646949983
1105	90-91	0.06034386736286024
1105	92-93	0.1002318344263955
1105	94-95	0.09778484543780408
1105	96-97	0.09145905168489676
1105	98-99	0.10926130363186815
1105	100	0.20977043972022003
1106	1	0.05487769563251277
1106	2	0.0740151400735769
1106	3	0.022811609178418735
1106	4	0.038179681299048696
1106	5	0.04035187296481979
1106	6	0.020729548922332697
1106	7	-0.046583506064848734
1106	8	0.04239538867052062
1106	9	-0.0212266201311877
1106	10-11	-0.04141513664330887
1106	12-13	-0.04511010367427559
1106	14-15	0.025297463405721032
1106	16-17	0.008725834474759608
1106	18-19	0.019056921125098825
1106	20-21	0.0036318927828844494
1106	22-23	-0.016683019764180074
1106	24-25	0.10832900878586571
1106	26-27	0.07048201674371768
1106	28-29	0.07612570653965633
1106	30-31	0.08573942008973745
1106	32-33	0.09604262162991262
1106	34-35	0.048551692023266924
1106	36-37	0.07064845709961531
1106	38-39	0.06581410588579217
1106	40-41	0.08527930261607253
1106	42-43	0.11549365655935873
1106	44-45	0.1144155409918568
1106	46-47	0.05990110132045601
1106	48-49	0.06164700941183554
1106	50-51	0.07827181080644152
1106	52-53	0.0109941875750863
1106	54-55	-0.005844923919241296
1106	56-57	0.07056782182153398
1106	58-59	0.03746245440510165
1106	60-61	-0.004471695184030011
1106	62-63	0.08561857388483674
1106	64-65	0.07583216490277778
1106	66-67	0.050984644997711825
1106	68-69	-0.018022146808156947
1106	70-71	0.008020987997198858
1106	72-73	0.04330905078064262
1106	74-75	0.013804427322462232
1106	76-77	-0.015937711088874096
1106	78-79	-0.0797357674764001
1106	80-81	-0.03987136500686006
1106	82-83	-0.058046714026318114
1106	84-85	-0.016742625083203677
1106	86-87	0.04905630660105942
1106	88-89	0.1479235736892619
1106	90-91	0.13265541883361465
1106	92-93	0.1377628294197848
1106	94-95	0.17974820693372706
1106	96-97	0.12590028641412943
1106	98-99	0.0989574650964613
1106	100	0.12405993038204599
1107	1	0.12972127217466678
1107	2	0.12291467176288862
1107	3	0.12984590890376424
1107	4	0.09443617167701035
1107	5	0.06056679309285329
1107	6	0.11426541399584522
1107	7	0.09235013331294795
1107	8	0.10479906056345101
1107	9	0.0636860541465154
1107	10-11	0.02541894626106256
1107	12-13	-0.015344240000629839
1107	14-15	0.030447024185839666
1107	16-17	-0.004688543385626076
1107	18-19	0.06566297114206066
1107	20-21	-0.01517821038800804
1107	22-23	0.02229818589798782
1107	24-25	-0.08383390033285565
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2216	1	0.004720971052833534
2216	2	-0.02176116244708126
2216	3	0.018992882096821262
2216	4	-0.018920160179789036
2216	5	0.06467920869896915
2216	6	-0.024305106349871153
2216	7	0.013257070859935993
2216	8	0.04276817404259958
2216	9	-0.0233941117549179
2216	10-11	0.008160607943899834
2216	12-13	0.014718496039272111
2216	14-15	-0.059234432866851705
2216	16-17	-0.03480958083888197
2216	18-19	-0.04410927129089259
2216	20-21	-0.03669109381700508
2216	22-23	-0.03679286400460313
2216	24-25	-0.10223637483163373
2216	26-27	-0.13636538049958347
2216	28-29	-0.060044124519009756
2216	30-31	-0.08445739106514338
2216	32-33	-0.05300437383549905
2216	34-35	-0.03870382634894298
2216	36-37	-0.009209833310755755
2216	38-39	-0.05440523393458818
2216	40-41	-0.04564365744640497
2216	42-43	-0.03286454270060091
2216	44-45	0.03158899074539079
2216	46-47	0.09524610744779949
2216	48-49	0.11314705708624473
2216	50-51	0.12721956165026427
2216	52-53	0.08503766979622895
2216	54-55	0.11479632488873648
2216	56-57	0.03921150409194496
2216	58-59	0.07049334397389373
2216	60-61	0.13600526464682616
2216	62-63	0.16087750861802874
2216	64-65	0.1669463912254514
2216	66-67	0.17401590155508018
2216	68-69	0.18321309580773004
2216	70-71	0.11061530736361647
2216	72-73	0.20513014696314968
2216	74-75	0.18689733668127673
2216	76-77	0.13404755608882368
2216	78-79	0.18007016930754105
2216	80-81	0.21396616970628202
2216	82-83	0.20923956659821386
2216	84-85	0.14320231128647265
2216	86-87	0.1278303184054721
2216	88-89	0.13715252302655045
2216	90-91	0.08106848309825665
2216	92-93	0.13899247556651062
2216	94-95	0.1281709680508918
2216	96-97	0.15086750785584613
2216	98-99	0.14629447283360975
2216	100	0.1500882858542525
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	28320.0
3	4776.0
4	3067.0
5	2531.0
6	2142.0
7	2488.0
8	2899.0
9	3207.0
10	3582.0
11	3924.0
12	4413.0
13	4746.0
14	5386.0
15	5585.0
16	5686.0
17	5759.0
18	5738.0
19	5666.0
20	5719.0
21	5964.0
22	6848.0
23	9283.0
24	14520.0
25	20143.0
26	21864.0
27	18526.0
28	18875.0
29	24112.0
30	29910.0
31	36118.0
32	47320.0
33	64482.0
34	90491.0
35	136652.0
36	250790.0
37	555159.0
38	991195.0
39	483409.0
40	1167.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.293823665564624	20.357621735398098	13.82169481991807	23.526859779119206
2	14.830800851501138	20.288124222202875	41.466056890791734	23.41501803550425
3	17.795877947723277	26.00664327574781	30.33842214352451	25.8590566330044
4	11.860649496325125	17.12774319267918	40.04996492970931	30.961642381286385
5	12.472762294793935	38.48333361195085	36.31898726058368	12.724916832671532
6	29.94429031108413	39.213379874597784	18.19969203643042	12.64263777788767
7	26.546756431667294	33.005689489535165	23.156798757540713	17.290755321256825
8	24.443562224953013	37.31044928732922	20.768288120152835	17.47770036756493
9	24.546397459409665	17.42310577004738	21.64246122929128	36.38803554125168
10-11	23.2667012981343	27.582394980870262	29.45639965304863	19.694504067946802
12-13	23.94220763518429	24.83535864342821	29.453399904978856	21.769033816408644
14-15	21.252625425235326	26.89864545452685	26.47523488133658	25.373494238901245
16-17	20.967261399742245	30.29228219274659	27.031417261749347	21.709039145761817
18-19	20.925337692764476	29.334921847100887	28.898853671073283	20.840886789061358
20-21	22.082893180828236	28.52483278752717	28.412016171916495	20.980257859728102
22-23	21.65733821035864	28.347564622319027	28.430597291214788	21.56449987610755
24-25	21.58566822308929	28.45831615060495	28.348363743777977	21.607651882527783
26-27	21.38180951221284	28.419887218852928	28.43046066456494	21.76784260436929
28-29	21.404931067279175	28.455576548907775	28.503873196741818	21.635619187071235
30-31	21.595200312429917	28.890387749063525	28.166119942732553	21.34829199577401
32-33	21.27167452608959	28.517511021668128	28.661999334862582	21.5488151173797
34-35	21.40470428938559	28.397103688431635	28.59399966916837	21.604192353014405
36-37	21.694920745624618	28.67520584140258	28.147406061546516	21.482467351426287
38-39	21.45925813376743	28.819906471629164	28.308882023388744	21.411953371214658
40-41	21.297155534183553	28.56576239220922	28.57000837259586	21.56707370101136
42-43	21.278894865243835	28.41569488611551	28.55013303135338	21.755277217287272
44-45	21.405308752434824	28.582678064951672	28.457311253632305	21.554701928981203
46-47	21.432053697537498	28.710646249925187	28.399071482638554	21.45822856989876
48-49	21.416486946225735	28.49464180727148	28.385019158812852	21.703852087689935
50-51	21.42658491198879	28.48422646152629	28.369554152356784	21.719634474128135
52-53	21.5245257331733	28.41459070576333	28.44606838500237	21.614815176061004
54-55	21.55394680506233	28.840606670621384	28.228933262241696	21.376513262074596
56-57	21.391528145818732	28.564216001299968	28.284869579510573	21.759386273370733
58-59	21.269427065678435	28.226800910387407	28.6474322205391	21.856339803395063
60-61	21.520947142520505	28.60659530882495	28.295753211495057	21.576704337159494
62-63	21.39057930858093	28.58498657231766	28.312408883584126	21.712025235517284
64-65	21.382979702925223	28.38826514727051	28.392152757150903	21.836602392653372
66-67	21.24756365196358	28.377906716025358	28.349806976271918	22.024722655739144
68-69	21.60453504485375	28.53556525307998	28.278571193509944	21.581328508556325
70-71	21.581576072055555	28.345957787535415	28.306519156296332	21.765946984112695
72-73	21.35029728924655	28.441793764760664	28.431989553997074	21.775919391995707
74-75	21.460398124538987	28.53854472055133	28.265031463999275	21.736025690910413
76-77	21.50119332189886	28.45431180784218	28.20117103609873	21.843323834160227
78-79	21.547825182510067	28.57815642453338	28.28208217066556	21.591936222290993
80-81	21.557085531361565	28.347172570380952	28.376551607014548	21.71919029124293
82-83	21.33753536147178	28.423913775555704	28.542213042008175	21.69633782096434
84-85	21.39720612655827	28.3705337694068	28.363900933047397	21.86835917098753
86-87	21.57032384548978	28.41106512870083	28.277914876254158	21.740696149555237
88-89	21.338673210463327	28.35966364125851	28.286711469443365	22.014951678834795
90-91	21.39912125404171	28.23341938264082	28.43372076107662	21.93373860224085
92-93	21.514924348989602	28.410471060486497	28.26543040450132	21.809174186022577
94-95	21.30495337185708	28.159281804818725	28.459015774840186	22.076749048484007
96-97	21.338521659724734	28.14928919219494	28.464900755848614	22.047288392231714
98-99	21.311281401902676	28.30264857465647	28.3378089979468	22.04826102549405
100	21.51593175593719	28.182166202888055	28.265419952908633	22.036482088266126
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	255.0
1	233.5
2	215.0
3	211.5
4	182.5
5	152.5
6	133.5
7	129.5
8	158.0
9	163.0
10	156.0
11	171.5
12	187.5
13	220.5
14	274.0
15	322.0
16	389.5
17	553.5
18	831.0
19	1217.0
20	1819.0
21	2816.0
22	4376.0
23	6767.5
24	9925.0
25	13790.5
26	19208.5
27	26531.0
28	35753.5
29	45427.5
30	54218.0
31	63528.5
32	72988.0
33	81908.5
34	90530.5
35	97573.0
36	103054.5
37	107615.0
38	110708.0
39	111404.5
40	112230.0
41	112642.0
42	110375.0
43	107674.0
44	104485.5
45	101335.5
46	98169.0
47	93954.5
48	90552.0
49	88244.5
50	85257.5
51	81732.5
52	75931.5
53	70770.0
54	68050.5
55	64433.0
56	58629.5
57	52672.0
58	47680.0
59	42412.0
60	37097.5
61	31670.0
62	27086.5
63	22884.0
64	19026.0
65	15492.0
66	12170.0
67	8994.5
68	6553.0
69	5024.0
70	3649.5
71	2675.5
72	1975.5
73	1400.5
74	1060.5
75	786.5
76	518.5
77	355.0
78	268.5
79	169.5
80	96.0
81	70.5
82	57.5
83	41.5
84	25.5
85	19.5
86	17.0
87	15.0
88	13.5
89	10.0
90	6.0
91	4.0
92	2.0
93	3.0
94	2.5
95	1.0
96	1.5
97	2.5
98	4.5
99	3.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3310869842473662
2	0.11993335293006353
3	0.06752005652588167
4	0.04112585261121883
5	0.04511567413320275
6	0.010128008478882249
7	0.007263521232329694
8	0.01578878089468849
9	0.018585066063942175
10-11	0.021790563696989083
12-13	0.016470801667677195
14-15	0.018960177489085964
16-17	0.020000259167893737
18-19	0.019812703455321844
20-21	0.024314040557047285
22-23	0.01648785218700191
24-25	0.025200667561932597
26-27	0.02008551176451732
28-29	0.01982975397464656
30-31	0.020614077863583567
32-33	0.02513246548463373
34-35	0.01558417466279188
36-37	0.009872250689011486
38-39	0.013998476365593144
40-41	0.009445987705893546
42-43	0.008815118490878996
44-45	0.009087926800074477
46-47	0.008883320568177866
48-49	0.004433135024426574
50-51	0.006598550978665709
52-53	0.007689784215447634
54-55	0.003290750229670495
56-57	0.0027792346499289676
58-59	0.0028644872465525555
60-61	0.0035806090581906942
62-63	0.002966790362500861
64-65	0.002352971666811028
66-67	0.001722102451796477
68-69	0.003358952306969366
70-71	0.0019778602416672406
72-73	0.0016539003744976066
74-75	0.0021483654349144167
76-77	0.0034612554229176713
78-79	0.0030520429591244487
80-81	0.003376002826294083
82-83	0.00233592114748631
84-85	0.0027792346499289676
86-87	0.002063112838290829
88-89	0.598081066353119
90-91	0.6088399440470158
92-93	0.6057026484912678
94-95	0.5925737486112352
96-97	0.5909027977174128
98-99	0.5883281692993805
100	0.5891977457849411
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2932462.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.57019500563726
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.18102581386952	50.60774913350548
2	14.016022257561724	18.38066625263349
3	4.295689005805922	8.450074973827991
4	1.7820165710075595	4.673886962569708
5	0.8657505859440081	2.838371737329666
6	0.500637500576936	1.9696139123978713
7	0.30563351181974635	1.4028314279194956
8	0.19936264586928212	1.0457798053190905
9	0.15313575481742842	0.9037027175142813
>10	0.6609661936220377	7.419873717557915
>50	0.031417396066892735	1.3961922734039596
>100	0.008342763038898235	0.911257086021054
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.4101038649435185E-5	0.0
8	0.0	0.0	0.0	1.3640415459774074E-4	0.0
9	0.0	0.0	0.0	3.4101038649435187E-4	0.0
10-11	0.0	0.0	0.0	3.4101038649435187E-4	0.0
12-13	0.0	0.0	0.0	3.75111425143787E-4	0.0
14-15	0.0	0.0	0.0	3.75111425143787E-4	0.0
16-17	0.0	0.0	0.0	3.75111425143787E-4	0.0
18-19	0.0	0.0	0.0	3.921619444685046E-4	0.0
20-21	0.0	0.0	0.0	4.944650604168101E-4	0.0
22-23	0.0	0.0	0.0	5.456166183909629E-4	0.0
24-25	0.0	0.0	0.0	6.820207729887037E-4	0.0
26-27	0.0	0.0	0.0	7.502228502875741E-4	0.0
28-29	0.0	0.0	0.0	0.0012276373913796667	0.0
30-31	0.0	0.0	0.0	0.003955720483334481	0.0
32-33	0.0	0.0	0.0	0.011372696389586634	0.0
34-35	0.0	0.0	0.0	0.020648178902233007	0.0
36-37	0.0	0.0	0.0	0.038585325231835915	0.0
38-39	0.0	0.0	0.0	0.0719190905116588	0.0
40-41	0.0	0.0	0.0	0.11099888080391153	0.0
42-43	0.0	0.0	0.0	0.14968650915169573	0.0
44-45	0.0	0.0	0.0	0.19400080887663676	0.0
46-47	0.0	0.0	0.0	0.25192142302270243	0.0
48-49	0.0	0.0	0.0	0.31645763866675847	0.0
50-51	0.0	0.0	0.0	0.38465971596562887	0.0
52-53	0.0	0.0	0.0	0.4539700770206059	0.0
54-55	0.0	0.0	0.0	0.5282762402377251	0.0
56-57	0.0	0.0	0.0	0.6204854487457978	0.0
58-59	0.0	0.0	0.0	0.7246811723391471	0.0
60-61	0.0	0.0	0.0	0.8321335451235173	0.0
62-63	0.0	0.0	0.0	0.9439338003356906	0.0
64-65	0.0	0.0	0.0	1.0655210536402517	0.0
66-67	0.0	0.0	0.0	1.2014477936969004	0.0
68-69	0.0	0.0	0.0	1.3598641687428517	0.0
70-71	0.0	0.0	0.0	1.528220996555113	0.0
72-73	0.0	0.0	0.0	1.705068982991084	0.0
74-75	0.0	0.0	0.0	1.8842528905745413	0.0
76-77	0.0	0.0	0.0	2.077810385948735	0.0
78-79	0.0	0.0	0.0	2.3034740092113726	0.0
80-81	0.0	0.0	0.0	2.5479443552891734	0.0
82-83	0.0	0.0	0.0	2.8018606890728677	0.0
84-85	0.0	0.0	0.0	3.0546175875424817	0.0
86-87	0.0	0.0	0.0	3.324544358972086	0.0
88	0.0	0.0	0.0	3.5481789704350817	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2490	0.0	34.99785	1
TCAACGC	3660	0.0	33.63095	4
GTATCAA	3885	0.0	32.979713	1
ATCAACG	3740	0.0	32.917755	3
CAACGCA	3960	0.0	31.20126	5
AACGCAG	4280	0.0	28.858116	6
GTACATG	7970	0.0	27.305656	1
TACATGG	7890	0.0	26.575907	2
TATCAAC	4810	0.0	26.390585	2
ACGCAGA	4985	0.0	24.117416	7
ACATGGG	8420	0.0	24.108608	3
CGCAGAG	5075	0.0	23.227425	8
TATACCG	165	6.886135E-5	19.930841	5
TAGACCG	120	0.0050785514	19.574934	5
GTATTAG	1525	0.0	18.842085	1
CATGGGG	5385	0.0	18.670118	4
GCAGAGT	6365	0.0	18.15098	9
GTATAAC	810	0.0	17.446377	1
GAGTACA	5750	0.0	16.875956	1
AGAGTAC	5225	0.0	16.538736	10-11
>>END_MODULE