FastQCFastQC Report
Wed 25 May 2016
SRR1033163_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033163_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2932462
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48400.1650490270632663No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40410.13780229718236758No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43050.052.7960131
GTATCAA79100.041.465171
TCAACGC90850.035.4504854
ATCAACG91800.035.032413
CAACGCA93750.034.4040345
AACGCAG96950.033.2046856
TATCAAC100450.032.1571962
ACGCAGA112050.028.6460957
CGCAGAG113050.028.1423198
TATAACG2051.0786607E-925.2295912
GCAGAGT127350.024.7608389
GTACATG79200.023.8257371
TACATGG78300.022.5786232
GAGTACT84550.022.12132812-13
AGAGTAC114950.021.2177910-11
ACCGTTC3550.021.180368
ACATGGG81300.020.992913
CAGAGTA126000.020.75565110-11
GTGGTAT20350.020.580351
ATAACGC2751.4315447E-920.5165863
Produced by FastQC (version 0.11.5)