##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033163_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2932462
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.75656257438289	34.0	31.0	34.0	31.0	34.0
2	32.85571304930806	34.0	31.0	34.0	31.0	34.0
3	32.96069787093575	34.0	31.0	34.0	31.0	34.0
4	36.38512826423667	37.0	37.0	37.0	35.0	37.0
5	36.31477645746134	37.0	37.0	37.0	35.0	37.0
6	36.399755222744574	37.0	37.0	37.0	35.0	37.0
7	36.378001488169325	37.0	37.0	37.0	35.0	37.0
8	36.37037581390654	37.0	37.0	37.0	35.0	37.0
9	38.19220811727484	39.0	39.0	39.0	37.0	39.0
10-11	38.17739564911668	39.0	39.0	39.0	37.0	39.0
12-13	38.1400410303697	39.0	39.0	39.0	37.0	39.0
14-15	39.683870924840626	41.0	40.0	41.0	37.0	41.0
16-17	39.69503867398793	41.0	40.0	41.0	37.0	41.0
18-19	39.712977184359076	41.0	40.0	41.0	37.0	41.0
20-21	39.710344584175346	41.0	40.0	41.0	37.0	41.0
22-23	39.64248590433567	41.0	40.0	41.0	37.0	41.0
24-25	39.59704592932491	41.0	40.0	41.0	37.0	41.0
26-27	39.50433168443445	41.0	39.5	41.0	37.0	41.0
28-29	39.41656464772604	41.0	39.0	41.0	36.0	41.0
30-31	39.32324527990474	41.0	39.0	41.0	36.0	41.0
32-33	39.36343966264525	41.0	39.0	41.0	36.0	41.0
34-35	39.30897928089094	41.0	39.0	41.0	36.0	41.0
36-37	39.226199521085015	41.0	39.0	41.0	35.0	41.0
38-39	39.141647871310866	41.0	39.0	41.0	35.0	41.0
40-41	39.03471911997496	41.0	39.0	41.0	35.0	41.0
42-43	38.92555828515425	41.0	39.0	41.0	35.0	41.0
44-45	38.7905255038258	41.0	39.0	41.0	35.0	41.0
46-47	38.59190928987315	41.0	39.0	41.0	35.0	41.0
48-49	38.42298843088163	41.0	39.0	41.0	35.0	41.0
50-51	38.30415347240646	41.0	38.5	41.0	35.0	41.0
52-53	38.18037045322326	40.5	38.0	41.0	34.0	41.0
54-55	38.054930976087675	40.0	38.0	41.0	34.0	41.0
56-57	37.91014393366393	40.0	37.5	41.0	34.0	41.0
58-59	37.7350170266486	40.0	37.0	41.0	34.0	41.0
60-61	37.55820723337591	40.0	37.0	41.0	33.5	41.0
62-63	37.33887787804241	40.0	36.0	41.0	33.0	41.0
64-65	37.09908193183748	39.0	36.0	41.0	33.0	41.0
66-67	36.83060820566473	39.0	35.0	41.0	33.0	41.0
68-69	36.519472034079214	39.0	35.0	41.0	32.0	41.0
70-71	36.19125004859398	37.5	35.0	40.0	32.0	41.0
72-73	35.84235021630289	37.0	35.0	40.0	32.0	41.0
74-75	35.50219695941499	36.5	35.0	39.0	31.0	41.0
76-77	35.15305620328584	36.0	35.0	39.0	31.0	41.0
78-79	34.80005060594135	36.0	35.0	38.5	31.0	40.0
80-81	34.445536037636636	35.0	35.0	37.0	31.0	39.0
82-83	34.106295665553375	35.0	35.0	37.0	31.0	39.0
84-85	33.819312236612106	35.0	34.0	36.5	31.0	38.0
86-87	33.57034277000009	35.0	34.0	36.0	30.5	37.0
88-89	33.3401556439606	35.0	34.0	36.0	30.0	37.0
90-91	33.1324283145016	35.0	34.0	35.5	30.0	36.5
92-93	32.9621712063106	35.0	34.0	35.0	30.0	36.0
94-95	32.808772458091525	35.0	34.0	35.0	30.0	36.0
96-97	32.67970241387612	35.0	34.0	35.0	30.0	36.0
98-99	32.574035912485826	35.0	34.0	35.0	30.0	36.0
100	32.47826161089215	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.09457949867593385
1101	2	0.11393310459374817
1101	3	0.14426731068034826
1101	4	0.12282358770467283
1101	5	0.09148319340853561
1101	6	0.09436619867093299
1101	7	0.10140141607171671
1101	8	0.13540252137281072
1101	9	0.15543118368620412
1101	10-11	0.16108877446502845
1101	12-13	0.15370165223731647
1101	14-15	0.18705112289129744
1101	16-17	0.14359654832345825
1101	18-19	0.19009759175945362
1101	20-21	0.19891782850159956
1101	22-23	0.1874931190029301
1101	24-25	0.1987952005014364
1101	26-27	0.15950023868304442
1101	28-29	0.19288843370015485
1101	30-31	0.17649639413247087
1101	32-33	0.14146341023181463
1101	34-35	0.1950146496630225
1101	36-37	0.23060726771728213
1101	38-39	0.20445039976362978
1101	40-41	0.23193113383013042
1101	42-43	0.21020010632793173
1101	44-45	0.1856138705998518
1101	46-47	0.232203399808391
1101	48-49	0.237076277806068
1101	50-51	0.20145150557939928
1101	52-53	0.18250628255547952
1101	54-55	0.23894492857606764
1101	56-57	0.2578755893435485
1101	58-59	0.21998370417949786
1101	60-61	0.20534358767763905
1101	62-63	0.18553566662676246
1101	64-65	0.27649645492005703
1101	66-67	0.22919978719358625
1101	68-69	0.2466283918664658
1101	70-71	0.17517421539537992
1101	72-73	0.23022369375716067
1101	74-75	0.25183644157678486
1101	76-77	0.19410532740908337
1101	78-79	0.13444324878230418
1101	80-81	0.12320903818126538
1101	82-83	0.1275979134610381
1101	84-85	0.13863770534254627
1101	86-87	0.13191677498849685
1101	88-89	0.12260096834764767
1101	90-91	0.14994349198641999
1101	92-93	0.087581775028994
1101	94-95	0.06561077576186136
1101	96-97	0.13819516821857292
1101	98-99	0.10189859629157638
1101	100	0.1443917164698192
1102	1	0.08415435756682399
1102	2	0.15120605845544333
1102	3	0.13231671749259988
1102	4	0.06745813933209632
1102	5	0.028942235164052477
1102	6	0.06157341378238357
1102	7	0.07328375166146373
1102	8	0.11096915873982027
1102	9	0.1161954702964394
1102	10-11	0.10378741509629208
1102	12-13	0.1260706474328117
1102	14-15	0.212443421593683
1102	16-17	0.12391774306394865
1102	18-19	0.1475008976407608
1102	20-21	0.13821263862644884
1102	22-23	0.17973856780221098
1102	24-25	0.14212924639960534
1102	26-27	0.177621918986425
1102	28-29	0.1693261501102583
1102	30-31	0.2105816241977223
1102	32-33	0.25377881983283146
1102	34-35	0.2765283806794443
1102	36-37	0.22239808936686245
1102	38-39	0.10605688286904069
1102	40-41	0.15302623510573454
1102	42-43	0.15481020661378864
1102	44-45	0.20194221280308966
1102	46-47	0.18553447858420213
1102	48-49	0.18987766740937673
1102	50-51	0.17546680121199643
1102	52-53	0.22787994190429828
1102	54-55	0.18251485800458056
1102	56-57	0.16885104145514163
1102	58-59	0.1987674908544932
1102	60-61	0.18539578769608056
1102	62-63	0.22207795687005216
1102	64-65	0.14163091542427964
1102	66-67	0.21113213356769478
1102	68-69	0.2253191076261487
1102	70-71	0.2549309856752089
1102	72-73	0.16191953033493434
1102	74-75	0.15152355429945175
1102	76-77	0.1329623806113034
1102	78-79	0.17858699965765368
1102	80-81	0.16964222569949783
1102	82-83	0.18206561404789312
1102	84-85	0.23959783401413404
1102	86-87	0.24597886737249297
1102	88-89	0.2256998402838022
1102	90-91	0.20551500883437512
1102	92-93	0.22324767645257282
1102	94-95	0.17681032322026624
1102	96-97	0.19233867877559163
1102	98-99	0.20552068498630405
1102	100	0.2211752215947982
1103	1	0.10000965100717707
1103	2	0.14984396595929894
1103	3	0.13264684040299812
1103	4	0.08832566476564807
1103	5	0.05965171760916377
1103	6	0.11285921567911572
1103	7	0.08533860574311092
1103	8	0.07366392790204657
1103	9	0.08127088191005072
1103	10-11	0.13162509661356125
1103	12-13	0.11800679146301718
1103	14-15	0.17539115079090806
1103	16-17	0.1627648894466276
1103	18-19	0.12576310916902855
1103	20-21	0.12258480792313264
1103	22-23	0.12667533584523483
1103	24-25	0.14678451622140187
1103	26-27	0.19058796597928307
1103	28-29	0.13961911406379102
1103	30-31	0.21549387994367208
1103	32-33	0.2074287580354337
1103	34-35	0.20773130293818554
1103	36-37	0.22250880131851858
1103	38-39	0.2095718866869234
1103	40-41	0.2437778283513623
1103	42-43	0.2547502208654322
1103	44-45	0.2645481440113784
1103	46-47	0.2701982564517422
1103	48-49	0.1848084019865297
1103	50-51	0.2635022612436444
1103	52-53	0.2478751913551065
1103	54-55	0.194781742248189
1103	56-57	0.17165641521198438
1103	58-59	0.20846787076422402
1103	60-61	0.21929176140434237
1103	62-63	0.1398538678281227
1103	64-65	0.2048400590237165
1103	66-67	0.20921983622088902
1103	68-69	0.1551666472039983
1103	70-71	0.18375594940865625
1103	72-73	0.19401593159262376
1103	74-75	0.17845675272076278
1103	76-77	0.08175843846065334
1103	78-79	0.14340005749492235
1103	80-81	0.16297602424668867
1103	82-83	0.1580176361849155
1103	84-85	0.14021339246534836
1103	86-87	0.1542462991370357
1103	88-89	0.15824002872225407
1103	90-91	0.11195133201665897
1103	92-93	0.13290672390036207
1103	94-95	0.13333139136918248
1103	96-97	0.11672241579378095
1103	98-99	0.11011583486344989
1103	100	0.08595634071146918
1104	1	0.06833717430387765
1104	2	0.08786328931790166
1104	3	0.10491163389019675
1104	4	0.08786174420605875
1104	5	-0.011147223207871093
1104	6	0.0247234897690376
1104	7	0.06860096923728776
1104	8	0.10563428925262741
1104	9	0.1034635415795222
1104	10-11	0.07502174756558588
1104	12-13	0.11060943536526224
1104	14-15	0.12538091435025223
1104	16-17	0.10395066634178107
1104	18-19	0.10103485357961262
1104	20-21	0.09090298184349876
1104	22-23	0.07316060395972812
1104	24-25	0.07427779354108566
1104	26-27	0.03728700251478756
1104	28-29	0.05595534692408677
1104	30-31	0.021930615783517737
1104	32-33	0.0579615739239685
1104	34-35	0.032293043882170025
1104	36-37	0.07592891300977556
1104	38-39	0.06201833968279402
1104	40-41	0.17199373924392347
1104	42-43	0.13096312644039187
1104	44-45	0.14940139400384567
1104	46-47	0.13032465889339306
1104	48-49	0.05880423813476199
1104	50-51	0.1056149217917266
1104	52-53	0.11114898249059735
1104	54-55	0.10568587481333935
1104	56-57	0.10782070402999011
1104	58-59	0.07690927622330435
1104	60-61	0.0756303710338031
1104	62-63	0.036144756213182916
1104	64-65	0.09182037661527431
1104	66-67	0.13110658542302644
1104	68-69	0.13023467658919685
1104	70-71	0.10831470531079646
1104	72-73	0.1308239756608529
1104	74-75	0.1388327440855477
1104	76-77	0.046362769756363775
1104	78-79	0.11006913660106932
1104	80-81	0.08701718515762735
1104	82-83	0.09046290079620434
1104	84-85	0.08383994796079719
1104	86-87	0.07519729404302922
1104	88-89	0.13701739500857713
1104	90-91	0.11395668875871934
1104	92-93	0.13608610323131387
1104	94-95	0.09090169684878902
1104	96-97	0.1098931187908434
1104	98-99	0.1746923151555535
1104	100	0.19775100137275814
1105	1	0.02566990175260031
1105	2	0.05131123881078281
1105	3	0.07854704910041477
1105	4	0.024427507782000646
1105	5	0.025651393654364085
1105	6	0.031857512161671764
1105	7	-0.0404687469290721
1105	8	-0.01559300351870263
1105	9	0.006557317459574108
1105	10-11	-0.024828215328469128
1105	12-13	-0.018172817234841432
1105	14-15	0.05853481262617066
1105	16-17	0.03120794502360269
1105	18-19	0.004433274694513045
1105	20-21	0.02778307046757078
1105	22-23	0.11360004059459072
1105	24-25	0.02071078234476431
1105	26-27	-0.01014448591782724
1105	28-29	0.03935554267142294
1105	30-31	0.04651492157957904
1105	32-33	-9.137393255400639E-4
1105	34-35	0.013969829538837075
1105	36-37	-0.03302424207159049
1105	38-39	0.05668866137525441
1105	40-41	0.14291020245525488
1105	42-43	0.09130909910030027
1105	44-45	0.11849697678238869
1105	46-47	0.199889981500057
1105	48-49	0.17185007205067393
1105	50-51	0.14314960778933994
1105	52-53	0.15090733670723466
1105	54-55	0.19484179509469612
1105	56-57	0.11467992727875043
1105	58-59	0.06751246303294778
1105	60-61	0.022626402310358174
1105	62-63	0.04976479127562072
1105	64-65	-0.018660575663247414
1105	66-67	0.013458134730917948
1105	68-69	0.009758375998728752
1105	70-71	0.07264983657982071
1105	72-73	0.07169470175440296
1105	74-75	0.03562240712048492
1105	76-77	0.009790418529718181
1105	78-79	0.05000239966612696
1105	80-81	0.07348601615883865
1105	82-83	0.06193622215524641
1105	84-85	0.04832242749874638
1105	86-87	0.04182304557297556
1105	88-89	0.03186531438648643
1105	90-91	0.0738518778915207
1105	92-93	0.10581289050128362
1105	94-95	0.06686228387568605
1105	96-97	0.14088478877373944
1105	98-99	0.08173276601975488
1105	100	0.10331205410622601
1106	1	0.05626549000304948
1106	2	0.06788374390705343
1106	3	0.04568751232999091
1106	4	0.029172086363452365
1106	5	0.027075308288530664
1106	6	0.0013423614585050814
1106	7	0.006076457838844362
1106	8	0.050549127205449906
1106	9	0.03542898633514113
1106	10-11	0.03488097279336699
1106	12-13	0.04254606564685304
1106	14-15	0.0940135938092439
1106	16-17	0.030468805896020967
1106	18-19	0.041031740352160284
1106	20-21	0.029758299207017558
1106	22-23	0.03419068716613083
1106	24-25	0.03970909011603396
1106	26-27	0.025519002752893982
1106	28-29	-0.011512111025616889
1106	30-31	0.06996450202640148
1106	32-33	0.005578256082145572
1106	34-35	0.04917182002030529
1106	36-37	0.03665057072173994
1106	38-39	0.03604627918714698
1106	40-41	0.04601475168551161
1106	42-43	-0.0038459170902740425
1106	44-45	0.06319428220184165
1106	46-47	0.04810113495696555
1106	48-49	-0.032361201985253274
1106	50-51	-0.042221450976960284
1106	52-53	-0.026604958231132514
1106	54-55	-0.06481895529847037
1106	56-57	-0.07126251633251712
1106	58-59	-0.06366100277929121
1106	60-61	-0.046396845738222225
1106	62-63	-0.058043885879705215
1106	64-65	-0.13142131492604392
1106	66-67	-0.11087141496309272
1106	68-69	-0.09352925753996999
1106	70-71	-0.07688384722280261
1106	72-73	-0.024476373089107994
1106	74-75	-0.0588637046490561
1106	76-77	-0.1340714648940633
1106	78-79	-0.11721561545881798
1106	80-81	-0.06404227353588254
1106	82-83	-0.10095001720133467
1106	84-85	-0.03963742174654783
1106	86-87	-0.05846085619943153
1106	88-89	-0.11314905203771985
1106	90-91	-0.07166191243288722
1106	92-93	-0.11836024139980594
1106	94-95	-0.1446657151926658
1106	96-97	-0.10221177853718189
1106	98-99	-0.06515083296532964
1106	100	-0.04415846586763905
1107	1	-0.10349236237520643
1107	2	-0.07432778502116832
1107	3	-0.02816620102579037
1107	4	-0.03988541003341339
1107	5	-0.05941960558741499
1107	6	-0.13264511505551724
1107	7	-0.06646567928768121
1107	8	-0.052842978482992464
1107	9	-0.040048657185884906
1107	10-11	-0.06039926464167422
1107	12-13	-0.0601961576735448
1107	14-15	-0.024606954915689982
1107	16-17	-0.03782502241362096
1107	18-19	-0.03403910733617721
1107	20-21	-0.009795638752315483
1107	22-23	-0.03992220338201946
1107	24-25	-0.03385011166525942
1107	26-27	0.017683553468962998
1107	28-29	-0.034342651282806
1107	30-31	-0.035259436126622745
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2216	6	-0.016267506331416826
2216	7	-0.05462387826045756
2216	8	-0.05379676340271544
2216	9	-0.06764591330456682
2216	10-11	-0.10830392367898867
2216	12-13	-0.08590788395790128
2216	14-15	-0.21486551354838213
2216	16-17	-0.1536030077576669
2216	18-19	-0.10498214357996716
2216	20-21	-0.10346946417324432
2216	22-23	-0.12635044579536725
2216	24-25	-0.18304875356140826
2216	26-27	-0.23593209563623674
2216	28-29	-0.20141296912673567
2216	30-31	-0.18487185111992943
2216	32-33	-0.16405470973834468
2216	34-35	-0.14899593518731535
2216	36-37	-0.17711000039261648
2216	38-39	-0.18216646856370033
2216	40-41	-0.23017012803580172
2216	42-43	-0.13054104945358347
2216	44-45	-0.030703686706083033
2216	46-47	-0.04682212159477217
2216	48-49	-0.10552568050920286
2216	50-51	-0.071766503214441
2216	52-53	0.016226422965957
2216	54-55	0.006974217784886605
2216	56-57	0.008186460342649582
2216	58-59	0.020683200789655132
2216	60-61	-0.015944956640034036
2216	62-63	0.05347324274714538
2216	64-65	-0.005106469675659753
2216	66-67	0.03171781771153093
2216	68-69	0.048582676872996444
2216	70-71	0.05595408137434532
2216	72-73	0.07160330238900059
2216	74-75	0.05068398257489548
2216	76-77	0.021656214411287067
2216	78-79	0.060015079397174986
2216	80-81	0.01602022619506016
2216	82-83	0.0213876693154873
2216	84-85	0.03609303576033085
2216	86-87	0.0345652484521608
2216	88-89	0.014132793182525916
2216	90-91	0.01901691455569221
2216	92-93	0.08645674148442595
2216	94-95	-0.0058925030056258265
2216	96-97	0.030459321573083287
2216	98-99	0.10858490312445213
2216	100	0.11687484096665202
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16.0
3	5.0
4	11.0
5	31.0
6	66.0
7	135.0
8	312.0
9	607.0
10	1040.0
11	1815.0
12	2754.0
13	4301.0
14	5121.0
15	6901.0
16	8655.0
17	12662.0
18	14807.0
19	7948.0
20	5212.0
21	4942.0
22	5102.0
23	5448.0
24	6141.0
25	7487.0
26	9621.0
27	12200.0
28	15843.0
29	20555.0
30	26262.0
31	33577.0
32	43336.0
33	57292.0
34	79039.0
35	120728.0
36	223347.0
37	529523.0
38	1063733.0
39	593403.0
40	2484.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.0795438332777	20.44418640688155	14.062705858340108	23.413563901500645
2	15.082268313135547	20.513351273028356	40.935037547524786	23.469342866311305
3	17.83245614094914	26.02308231104103	30.687388276472127	25.457073271537705
4	11.95759592477836	17.316213655866385	39.73422942013754	30.99196099921772
5	12.182239094569903	38.25736228257028	36.31576110465965	13.244637518200166
6	29.807874617215578	39.1672520682294	17.942648834084693	13.082224480470323
7	26.26123288871823	33.20147090267905	23.12443027756928	17.412865931033448
8	24.63098300296714	36.706879871561455	20.616494109198	18.04564301627341
9	24.850696872963525	16.501861749379447	21.78006361218596	36.86737776547107
10-11	23.40246718487927	27.130320825425414	29.36962469797028	20.09758729172503
12-13	24.41792937129279	24.47677071348239	29.623146011781227	21.48215390344359
14-15	21.462741927976133	26.342589332466254	27.204408860347044	24.990259879210566
16-17	20.569507806068756	29.958103463915304	27.62794539196075	21.844443338055193
18-19	20.396632928775315	28.490009836452984	30.43322507949385	20.680132155277846
20-21	21.55906879611739	27.680597395635477	30.11621292961341	20.64412087863372
22-23	21.104946206418195	27.51103638430471	30.237484950772554	21.14653245850454
24-25	21.02607043677841	27.66089352640676	30.150834137692268	21.16220189912256
26-27	20.824989837758796	27.660870324346003	30.19501954696865	21.319120290926552
28-29	20.862765653958952	27.67947441896869	30.239939240606482	21.217820686465878
30-31	21.060440090893305	28.074395099321166	29.9116038383693	20.953560971416234
32-33	20.835685523414167	27.739953234765906	30.29826054477575	21.12610069704417
34-35	20.935367507227344	27.65921049265327	30.240979678159352	21.16444232196004
36-37	21.25489677064276	27.934101307744115	29.70243905184501	21.108562869768114
38-39	21.05409920837415	28.066288090683596	29.808987177353114	21.07062552358914
40-41	21.004466132186256	27.882719044723576	29.883941965012884	21.228872858077285
42-43	21.058626758191334	27.795765759020707	29.69349446395598	21.45211301883198
44-45	21.198037300566874	27.96219086810276	29.556257404848285	21.283514426482082
46-47	21.268187751173524	28.2135118607279	29.267848049803767	21.250452338294814
48-49	21.268445471523343	28.127903153856753	29.076215590166417	21.527435784453488
50-51	21.33461244449431	28.15397897412716	28.893173812756746	21.618234768621786
52-53	21.39987879866767	28.15530139879433	28.91255395488405	21.53226584765395
54-55	21.430064623936435	28.505857077088343	28.7163708288574	21.347707470117825
56-57	21.250682676812655	28.197131316582833	28.822968837199546	21.72921716940496
58-59	21.092633315242587	27.869501781537785	29.20743265374534	21.830432249474285
60-61	21.344054065390395	28.198376169542932	28.861132793784307	21.596436971282365
62-63	21.226890744839856	28.187638984609688	28.893095109890126	21.692375160660326
64-65	21.24409969480313	27.976470905184357	28.961987907731274	21.81744149228124
66-67	21.08422898580625	28.009967765888792	28.91066436711251	21.99513888119245
68-69	21.499706728867427	28.18122450928237	28.779685176849313	21.539383585000888
70-71	21.4491197821894	28.068707595271675	28.741692969988296	21.740479652550626
72-73	21.298524238418906	28.18051241759456	28.746438134366738	21.7745252096198
74-75	21.382237638996497	28.175333373345406	28.612016308558587	21.83041267909951
76-77	21.425529121503995	28.12979227302721	28.56275714375839	21.88192146171041
78-79	21.478840038936585	28.266706405764037	28.61426436946039	21.64018918583899
80-81	21.419616207962804	28.125840275060337	28.65976287725526	21.794780639721598
82-83	21.243730926791272	28.144238386250954	28.827489359583502	21.784541327374267
84-85	21.254597257246914	28.084199075518633	28.654096993815763	22.00710667341869
86-87	21.38581965070617	28.17181277354385	28.51505707069441	21.92731050505557
88-89	21.13087910431576	28.09182182070902	28.59939873048653	22.177900344488695
90-91	21.11565034135163	28.004849171003187	28.674525824734182	22.204974662911003
92-93	21.179876080172264	28.112092217439926	28.59252494620132	22.115506756186488
94-95	20.989939511577642	27.875535983074972	28.70715801261875	22.427366492728634
96-97	20.957355969148107	27.8752120232078	28.712068562184268	22.455363445459824
98-99	20.917815814834086	27.977992553697202	28.620541374449182	22.483650257019526
100	21.054254070470478	27.875689437748896	28.48548421087809	22.58457228090253
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	45.0
1	37.5
2	71.5
3	140.0
4	119.0
5	93.5
6	208.5
7	494.0
8	911.5
9	1147.5
10	1042.5
11	879.0
12	924.5
13	1175.5
14	1403.5
15	1558.0
16	1752.5
17	2022.5
18	2335.5
19	2737.5
20	3371.0
21	4360.5
22	5815.5
23	8096.0
24	11149.0
25	14913.5
26	20032.0
27	26713.5
28	35566.0
29	44993.5
30	53800.5
31	63246.0
32	72511.5
33	80874.0
34	89186.5
35	96282.5
36	101312.0
37	106132.5
38	109737.0
39	110734.5
40	111306.5
41	111396.5
42	109807.0
43	107293.5
44	104568.0
45	102008.0
46	98423.5
47	93780.0
48	90364.0
49	88122.5
50	85357.0
51	81546.0
52	75301.5
53	70283.5
54	67541.0
55	63862.0
56	58238.5
57	52292.5
58	47252.5
59	42183.5
60	37044.5
61	31375.5
62	26661.0
63	22559.5
64	18622.0
65	14876.0
66	11470.5
67	8486.5
68	6302.0
69	4822.5
70	3350.5
71	2394.5
72	1761.0
73	1278.0
74	927.5
75	632.5
76	395.5
77	258.5
78	189.5
79	113.5
80	60.5
81	26.5
82	9.5
83	4.0
84	1.5
85	1.5
86	1.5
87	1.5
88	1.0
89	0.0
90	0.0
91	0.0
92	1.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09057235865289985
2	0.0015345467392245834
3	0.0
4	2.728083091954815E-4
5	0.03989821521983917
6	6.820207729887037E-5
7	0.0038534173673861758
8	3.069093478449167E-4
9	1.705051932471759E-4
10-11	3.5806090581906943E-4
12-13	0.0
14-15	1.1935363527302313E-4
16-17	0.0
18-19	8.525259662358795E-5
20-21	0.0
22-23	8.525259662358795E-5
24-25	8.525259662358795E-5
26-27	0.001295839468678537
28-29	0.008201299795189161
30-31	0.03795445601682136
32-33	0.027178527803599844
34-35	0.03024762128204901
36-37	0.027638891825367218
38-39	0.026394203914662837
40-41	0.032549441390885885
42-43	0.02456979834691805
44-45	0.02330805991688895
46-47	0.01619799335848171
48-49	0.010434917826727166
50-51	0.0074169759062521524
52-53	0.005149256836064713
54-55	0.00381931632873674
56-57	0.0024382242634346154
58-59	8.013744082617269E-4
60-61	4.4331350244265744E-4
62-63	0.001125334275431361
64-65	0.003154346075072755
66-67	3.2395986716963424E-4
68-69	7.502228502875741E-4
70-71	2.046062318966111E-4
72-73	3.410103864943518E-4
74-75	4.774145410920925E-4
76-77	2.3870727054604626E-4
78-79	5.1151557974152774E-5
80-81	4.944650604168101E-4
82-83	3.7511142514378706E-4
84-85	2.3870727054604626E-4
86-87	8.525259662358795E-5
88-89	0.0
90-91	6.820207729887037E-5
92-93	5.1151557974152774E-5
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2932462.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.56943885087609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.89007970655764	48.24289781294129
2	14.668375418485333	18.64920788414195
3	4.567117001110995	8.709871947807663
4	1.8977290184918567	4.825502751862048
5	0.9743286015919363	3.096876122977911
6	0.5375434746246024	2.0502802223901675
7	0.3228518199949692	1.4366456320344974
8	0.22798106829092216	1.1594102863901747
9	0.16333033829937804	0.9344536157713697
>10	0.7041767361212757	7.704977957789814
>50	0.03562670371218724	1.554145426493335
>100	0.010484545473050087	1.1677881979021183
>500	1.609573912846336E-4	0.055457283051089046
>1k	2.1460985459799918E-4	0.41248485844662336
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4840	0.1650490270632663	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4041	0.13780229718236758	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.3640415459774074E-4	0.0
10-11	0.0	0.0	0.0	2.216567512213287E-4	0.0
12-13	0.0	0.0	0.0	3.4101038649435187E-4	0.0
14-15	0.0	0.0	0.0	3.7511142514378706E-4	0.0
16-17	0.0	0.0	0.0	4.2626298311793976E-4	0.0
18-19	0.0	0.0	0.0	5.115155797415277E-4	0.0
20-21	0.0	0.0	0.0	5.967681763651158E-4	0.0
22-23	0.0	0.0	0.0	6.308692150145509E-4	0.0
24-25	0.0	0.0	0.0	7.161218116381389E-4	0.0
26-27	0.0	0.0	0.0	7.843238889370093E-4	0.0
28-29	0.0	0.0	0.0	0.0012276373913796667	0.0
30-31	0.0	0.0	0.0	0.0040580235992827875	0.0
32-33	0.0	0.0	0.0	0.01147499950553494	0.0
34-35	0.0	0.0	0.0	0.02073343149885659	0.0
36-37	0.0	0.0	0.0	0.03872172938643365	0.0
38-39	0.0	0.0	0.0	0.07227715141747787	0.0
40-41	0.0	0.0	0.0	0.1114592448256789	0.0
42-43	0.0	0.0	0.0	0.15047083304063275	0.0
44-45	0.0	0.0	0.0	0.19485333484287265	0.0
46-47	0.0	0.0	0.0	0.2531661109334068	0.0
48-49	0.0	0.0	0.0	0.31814564007990553	0.0
50-51	0.0	0.0	0.0	0.386825131919868	0.0
52-53	0.0	0.0	0.0	0.4565958569966124	0.0
54-55	0.0	0.0	0.0	0.5314987883900968	0.0
56-57	0.0	0.0	0.0	0.6244752702677818	0.0
58-59	0.0	0.0	0.0	0.7294041661920939	0.0
60-61	0.0	0.0	0.0	0.8369758926117372	0.0
62-63	0.0	0.0	0.0	0.9492024108070283	0.0
64-65	0.0	0.0	0.0	1.0714887354039029	0.0
66-67	0.0	0.0	0.0	1.2082168498688133	0.0
68-69	0.0	0.0	0.0	1.3676733065935722	0.0
70-71	0.0	0.0	0.0	1.5371554686812652	0.0
72-73	0.0	0.0	0.0	1.715214041989291	0.0
74-75	0.0	0.0	0.0	1.895847243715349	0.0
76-77	0.0	0.0	0.0	2.0901379114205056	0.0
78-79	0.0	0.0	0.0	2.318376163101176	0.0
80-81	0.0	0.0	0.0	2.566222512005271	0.0
82-83	0.0	0.0	0.0	2.82377060640513	0.0
84-85	0.0	0.0	0.0	3.080790134705923	0.0
86-87	0.0	0.0	0.0	3.3553887484305	0.0
88	0.0	0.0	0.0	3.583030231934804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4305	0.0	52.796013	1
GTATCAA	7910	0.0	41.46517	1
TCAACGC	9085	0.0	35.450485	4
ATCAACG	9180	0.0	35.03241	3
CAACGCA	9375	0.0	34.404034	5
AACGCAG	9695	0.0	33.204685	6
TATCAAC	10045	0.0	32.157196	2
ACGCAGA	11205	0.0	28.646095	7
CGCAGAG	11305	0.0	28.142319	8
TATAACG	205	1.0786607E-9	25.229591	2
GCAGAGT	12735	0.0	24.760838	9
GTACATG	7920	0.0	23.825737	1
TACATGG	7830	0.0	22.578623	2
GAGTACT	8455	0.0	22.121328	12-13
AGAGTAC	11495	0.0	21.21779	10-11
ACCGTTC	355	0.0	21.18036	8
ACATGGG	8130	0.0	20.99291	3
CAGAGTA	12600	0.0	20.755651	10-11
GTGGTAT	2035	0.0	20.58035	1
ATAACGC	275	1.4315447E-9	20.516586	3
>>END_MODULE