FastQCFastQC Report
Wed 25 May 2016
SRR1033162_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033162_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4094925
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50400.036.170081
TCAACGC51000.033.8978774
CAACGCA53900.032.074065
GGTATCA32250.031.987731
ATCAACG54400.031.9582183
AACGCAG58650.029.4652546
GTACATG121200.028.7217481
TACATGG121800.027.2126662
TATCAAC66450.027.1684882
ACATGGG126000.025.320893
ACGCAGA69800.024.4892987
CGCAGAG71300.023.9082328
CATGGGG84950.021.346114
GAGTACA90000.019.5224971
GCAGAGT89000.018.5202779
AGTACAT91300.017.9199872
TCTATAC18350.017.4121573
CATGGGA41000.017.3016644
TATACCG2756.6408757E-717.0829225
TAGCGCG1750.0023372616.1067544
Produced by FastQC (version 0.11.5)