##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033162_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4094925
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.7230877244394	33.0	31.0	34.0	30.0	34.0
2	31.87352149306764	34.0	31.0	34.0	30.0	34.0
3	31.98958515723731	34.0	31.0	34.0	30.0	34.0
4	35.352634053126735	37.0	35.0	37.0	33.0	37.0
5	35.42430252080319	37.0	35.0	37.0	35.0	37.0
6	35.535202964645265	37.0	37.0	37.0	35.0	37.0
7	35.480113066783886	37.0	37.0	37.0	35.0	37.0
8	35.47982026532843	37.0	37.0	37.0	35.0	37.0
9	37.16007155198203	39.0	38.0	39.0	35.0	39.0
10-11	37.146912947123575	39.0	38.0	39.0	35.0	39.0
12-13	37.095959755062665	39.0	38.0	39.0	35.0	39.0
14-15	38.564164423035834	41.0	39.0	41.0	35.0	41.0
16-17	38.56673382296379	41.0	39.0	41.0	35.0	41.0
18-19	38.55650250004579	41.0	39.0	41.0	35.0	41.0
20-21	38.57945554558386	41.0	39.0	41.0	34.5	41.0
22-23	38.538992777645504	41.0	39.0	41.0	34.0	41.0
24-25	38.525795344237075	41.0	39.0	41.0	34.0	41.0
26-27	38.475896628143374	41.0	39.0	41.0	34.0	41.0
28-29	38.44366294864986	40.5	39.0	41.0	34.0	41.0
30-31	38.43721850827549	41.0	39.0	41.0	34.0	41.0
32-33	38.50498079940414	41.0	39.0	41.0	34.0	41.0
34-35	38.47664474929333	41.0	39.0	41.0	34.0	41.0
36-37	38.40045776174166	41.0	39.0	41.0	34.0	41.0
38-39	38.32624236097121	41.0	39.0	41.0	34.0	41.0
40-41	38.26835021398438	41.0	39.0	41.0	33.5	41.0
42-43	38.1579776186377	40.5	38.0	41.0	33.0	41.0
44-45	38.04074592330751	40.0	38.0	41.0	33.0	41.0
46-47	37.918698633064096	40.0	38.0	41.0	33.0	41.0
48-49	37.80168757669554	40.0	38.0	41.0	33.0	41.0
50-51	37.67821693926018	40.0	38.0	41.0	33.0	41.0
52-53	37.53031716087596	40.0	37.0	41.0	32.0	41.0
54-55	37.378585932587285	40.0	37.0	41.0	32.0	41.0
56-57	37.21848153507085	40.0	36.5	41.0	32.0	41.0
58-59	37.01994200137976	40.0	36.0	41.0	31.0	41.0
60-61	36.80056399079348	39.5	35.5	41.0	31.0	41.0
62-63	36.5378549057675	39.0	35.0	41.0	31.0	41.0
64-65	36.26876682723127	39.0	35.0	41.0	31.0	41.0
66-67	35.97293991953455	38.0	35.0	40.5	30.5	41.0
68-69	35.66316428261812	37.5	35.0	40.0	30.0	41.0
70-71	35.310768206987916	37.0	35.0	40.0	30.0	41.0
72-73	34.93818043065502	36.5	34.5	39.0	29.5	41.0
74-75	34.56121162170247	36.0	34.0	39.0	29.0	41.0
76-77	34.226827597575046	35.5	34.0	38.5	29.0	40.0
78-79	33.854404048914205	35.0	34.0	37.0	29.0	39.0
80-81	33.4923728761821	35.0	34.0	37.0	29.0	39.0
82-83	33.26818671892648	35.0	34.0	36.5	29.0	39.0
84-85	32.99625316702992	35.0	34.0	36.0	29.0	37.0
86-87	32.74155814819564	35.0	34.0	36.0	28.0	37.0
88-89	32.380555443628396	35.0	34.0	35.0	27.0	37.0
90-91	32.19040006837732	35.0	34.0	35.0	27.0	36.0
92-93	32.01523715330562	35.0	34.0	35.0	27.0	36.0
94-95	31.84871676526432	35.0	33.5	35.0	26.0	36.0
96-97	31.67360464477371	35.0	33.0	35.0	26.0	36.0
98-99	31.481412846389127	35.0	33.0	35.0	25.0	35.0
100	31.319395837530603	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1126105192213096
1101	2	0.18474211134003937
1101	3	0.17109614229067915
1101	4	0.1350920875415227
1101	5	0.07978771934155304
1101	6	0.10236698559100432
1101	7	0.1350736574835878
1101	8	0.08361649982266783
1101	9	0.20056601592593637
1101	10-11	0.14782239911617268
1101	12-13	0.17746776203628656
1101	14-15	0.25701244612315577
1101	16-17	0.13220971614859423
1101	18-19	0.16598469661066417
1101	20-21	0.15884166532828914
1101	22-23	0.14862553835076397
1101	24-25	0.13732027433341187
1101	26-27	0.16520943415673628
1101	28-29	0.20646634535012964
1101	30-31	0.18708595570098652
1101	32-33	0.16922593377752548
1101	34-35	0.1679346585809398
1101	36-37	0.20614081127389028
1101	38-39	0.08860380765834464
1101	40-41	0.1124746136434851
1101	42-43	0.13854716646763165
1101	44-45	0.14051038310810782
1101	46-47	0.1543442423309358
1101	48-49	0.13356900655986692
1101	50-51	0.12549027671783364
1101	52-53	0.13534820883275955
1101	54-55	0.0728372540524731
1101	56-57	0.11618393315907838
1101	58-59	0.08781491617237691
1101	60-61	0.1020147239464606
1101	62-63	0.11076066927994788
1101	64-65	0.09051074087995659
1101	66-67	0.07322276819260765
1101	68-69	0.0540166826961368
1101	70-71	0.10306311915078226
1101	72-73	0.138445145260782
1101	74-75	0.15020747929657574
1101	76-77	0.07977987535648623
1101	78-79	0.03217083794390874
1101	80-81	0.09567551133530117
1101	82-83	0.06118580514208816
1101	84-85	-0.03676836141099926
1101	86-87	0.04782848744498125
1101	88-89	0.21615914994583818
1101	90-91	0.2636012818381488
1101	92-93	0.2530240249750051
1101	94-95	0.2884791718824786
1101	96-97	0.276721072332613
1101	98-99	0.3003758817716431
1101	100	0.38829595403225525
1102	1	0.21807099007461517
1102	2	0.20934209173587348
1102	3	0.23782618981233128
1102	4	0.2027833047120211
1102	5	0.08546822211328475
1102	6	0.12079368935724943
1102	7	0.08709482489231846
1102	8	0.16331831697076638
1102	9	0.15009044974136287
1102	10-11	0.1551050049236551
1102	12-13	0.1817453499667323
1102	14-15	0.2165681525168992
1102	16-17	0.21601532817664548
1102	18-19	0.23728539329576392
1102	20-21	0.26126624047929425
1102	22-23	0.21556464289319166
1102	24-25	0.2580446569898598
1102	26-27	0.30075712763563445
1102	28-29	0.2567111621662832
1102	30-31	0.28315727925011913
1102	32-33	0.34732497089790826
1102	34-35	0.2634091803544649
1102	36-37	0.3045740421324723
1102	38-39	0.24364283036420886
1102	40-41	0.24710415665675356
1102	42-43	0.28602687482585054
1102	44-45	0.2765459398694645
1102	46-47	0.28817588835453734
1102	48-49	0.288312870311195
1102	50-51	0.24842824823636533
1102	52-53	0.19105593748727046
1102	54-55	0.2732472441906424
1102	56-57	0.18040550302522718
1102	58-59	0.2314190045426443
1102	60-61	0.1528558816751584
1102	62-63	0.21057434393248542
1102	64-65	0.2593041422616764
1102	66-67	0.2327739104528419
1102	68-69	0.15963556330870432
1102	70-71	0.2436048519221643
1102	72-73	0.25115755069008827
1102	74-75	0.161527785863953
1102	76-77	0.08014886775217889
1102	78-79	0.11465324854886916
1102	80-81	0.08495917375876161
1102	82-83	0.07542750677819754
1102	84-85	0.12932303161212388
1102	86-87	0.16602236636983747
1102	88-89	0.2852965713019415
1102	90-91	0.2964121733863223
1102	92-93	0.31222063004790357
1102	94-95	0.2754672912776712
1102	96-97	0.21672401168545008
1102	98-99	0.22774259159204746
1102	100	0.25616651368020626
1103	1	0.12187793900615773
1103	2	0.1303811626636211
1103	3	0.14577305499003046
1103	4	0.15343858946623357
1103	5	0.0566413931612999
1103	6	0.1724645078258007
1103	7	0.1835419504628817
1103	8	0.1457318487400201
1103	9	0.18375270257405418
1103	10-11	0.17882202536663527
1103	12-13	0.20040880298476793
1103	14-15	0.2300356540769286
1103	16-17	0.18222926578911824
1103	18-19	0.22208961266206018
1103	20-21	0.22272477671723578
1103	22-23	0.18994931077589428
1103	24-25	0.2460713047280123
1103	26-27	0.2685361629631018
1103	28-29	0.24072474820955136
1103	30-31	0.27646332657344885
1103	32-33	0.2894249423328006
1103	34-35	0.2636627524321611
1103	36-37	0.34105960042997197
1103	38-39	0.26841112059962313
1103	40-41	0.2797493806818565
1103	42-43	0.26808564928604284
1103	44-45	0.24956130330760118
1103	46-47	0.24080388927083618
1103	48-49	0.30166731115377843
1103	50-51	0.24633667063864095
1103	52-53	0.17400744400077173
1103	54-55	0.2658029130437498
1103	56-57	0.17574962735901067
1103	58-59	0.19049393280023708
1103	60-61	0.11883197461649075
1103	62-63	0.20764149472110915
1103	64-65	0.1697797399495613
1103	66-67	0.1315511951814088
1103	68-69	0.09565041387809714
1103	70-71	0.08515680316957486
1103	72-73	0.15530712261992363
1103	74-75	0.1007446633291238
1103	76-77	0.1170180499249085
1103	78-79	0.11560930130294622
1103	80-81	0.15779646276068604
1103	82-83	0.15470114175882088
1103	84-85	0.1578850219996255
1103	86-87	0.18717834689050505
1103	88-89	0.314157488988954
1103	90-91	0.3354483272683879
1103	92-93	0.3348331389358634
1103	94-95	0.2723965189529949
1103	96-97	0.25684704659319735
1103	98-99	0.23589325533579952
1103	100	0.23297171012495355
1104	1	-0.05190728559930591
1104	2	-0.05513697269910267
1104	3	-0.023949119265040508
1104	4	0.0182909349436855
1104	5	-0.11585168357974851
1104	6	-0.09853751726014792
1104	7	-0.12729868405241973
1104	8	-0.09556051424980438
1104	9	-0.03131758819991859
1104	10-11	-0.044711386225834815
1104	12-13	-0.07381646936690345
1104	14-15	-0.13738472190414086
1104	16-17	-0.07563901873691492
1104	18-19	-0.07718243493066268
1104	20-21	-0.1380745042987641
1104	22-23	-0.12446064673974888
1104	24-25	-0.11025758306780631
1104	26-27	-0.052152723832676884
1104	28-29	-0.014429831025488227
1104	30-31	-0.0033076462643535365
1104	32-33	-0.010049395822250062
1104	34-35	-0.037695604637640656
1104	36-37	-0.0015708280707684708
1104	38-39	-0.0643215040989844
1104	40-41	-0.06781160591814483
1104	42-43	-0.06585579125184182
1104	44-45	-0.12947552499964132
1104	46-47	-0.11100705968222258
1104	48-49	-0.1934866982896466
1104	50-51	-0.19821487137448202
1104	52-53	-0.21025338509255675
1104	54-55	-0.20751050374661162
1104	56-57	-0.1984459635003759
1104	58-59	-0.23896920720511616
1104	60-61	-0.22695995434327898
1104	62-63	-0.24274458271124644
1104	64-65	-0.20354481103428412
1104	66-67	-0.17083666779184625
1104	68-69	-0.20781073592652177
1104	70-71	-0.12787598833843106
1104	72-73	-0.16836375120204394
1104	74-75	-0.11730312300133505
1104	76-77	-0.27410753273063193
1104	78-79	-0.22022775747920065
1104	80-81	-0.14464744910301874
1104	82-83	-0.13536471568745156
1104	84-85	-0.14514278092456578
1104	86-87	-0.10725928040414345
1104	88-89	0.014313731085280779
1104	90-91	-0.09358964934340719
1104	92-93	-0.05158788358427557
1104	94-95	-0.06367424160835
1104	96-97	-0.047510245896539516
1104	98-99	-0.05627686293085077
1104	100	-0.0013073610216700615
1105	1	0.10316198774322771
1105	2	0.12633392515905584
1105	3	0.1412972251514617
1105	4	0.1156080569883855
1105	5	0.05701162398598569
1105	6	0.11887465983350154
1105	7	-0.0037288137935291843
1105	8	0.05784205009157262
1105	9	-0.012474787462728898
1105	10-11	0.10258964871140819
1105	12-13	0.12078912838202882
1105	14-15	0.12415008692739349
1105	16-17	0.12978649936672326
1105	18-19	0.12083158343122591
1105	20-21	0.10579694277888763
1105	22-23	0.18156983127312998
1105	24-25	0.19441057095072267
1105	26-27	0.23349821133628268
1105	28-29	0.21017060191525871
1105	30-31	0.2264601349284021
1105	32-33	0.2220051535835168
1105	34-35	0.1893056879755548
1105	36-37	0.2213938706064198
1105	38-39	0.20313759372474038
1105	40-41	0.21640320212313924
1105	42-43	0.19344947149014047
1105	44-45	0.1893444856686557
1105	46-47	0.23164000329174428
1105	48-49	0.19300224817415312
1105	50-51	0.23139133316885818
1105	52-53	0.26806211130760005
1105	54-55	0.2213621145853537
1105	56-57	0.23628440811181406
1105	58-59	0.23398935297053924
1105	60-61	0.20343769355226726
1105	62-63	0.2794827902076591
1105	64-65	0.3203866663548567
1105	66-67	0.262079388552543
1105	68-69	0.1875705002971344
1105	70-71	0.23626570803293845
1105	72-73	0.203551334271296
1105	74-75	0.23125320021969742
1105	76-77	0.2530236562195043
1105	78-79	0.24884259115946605
1105	80-81	0.18957138071513668
1105	82-83	0.2512959677041593
1105	84-85	0.23001223387839076
1105	86-87	0.2613425665086879
1105	88-89	0.3322709647801503
1105	90-91	0.2843741179749273
1105	92-93	0.3056699419468103
1105	94-95	0.3524341036491947
1105	96-97	0.404706176500099
1105	98-99	0.42293810033692125
1105	100	0.4979373916178851
1106	1	0.08758912781015127
1106	2	0.11979349136179351
1106	3	0.09464991455138261
1106	4	0.08694852534040365
1106	5	0.07207102173425994
1106	6	0.07484851699545203
1106	7	0.04932260126452093
1106	8	0.09518022649093894
1106	9	0.06998529571166046
1106	10-11	0.07408527604908954
1106	12-13	0.0894173177799189
1106	14-15	0.12345190523682703
1106	16-17	0.06888960079171369
1106	18-19	0.09941323188239437
1106	20-21	0.11239028855732869
1106	22-23	0.06978982903246589
1106	24-25	0.1219375567360288
1106	26-27	0.08998971504889397
1106	28-29	0.1301610048696844
1106	30-31	0.06303695921068453
1106	32-33	0.09636254972012637
1106	34-35	0.04466788533454036
1106	36-37	0.08537471430955179
1106	38-39	0.07798928495093804
1106	40-41	0.08136494217144019
1106	42-43	0.1240578377922219
1106	44-45	0.09838642786230878
1106	46-47	0.05741630439331402
1106	48-49	0.08237335148344727
1106	50-51	0.08762907533902364
1106	52-53	-0.003193657380656134
1106	54-55	-0.0039788099975268665
1106	56-57	0.05557980139495555
1106	58-59	0.02039252606965647
1106	60-61	-0.001923356480595828
1106	62-63	0.04975651522462954
1106	64-65	0.03914659578754964
1106	66-67	0.09861875601140468
1106	68-69	0.06171422381583369
1106	70-71	0.06771762899431621
1106	72-73	0.05814408074056843
1106	74-75	0.06720500172417587
1106	76-77	0.11760671115752785
1106	78-79	0.054611211924985525
1106	80-81	0.10447420219254866
1106	82-83	0.07300155572229272
1106	84-85	0.062206069902536854
1106	86-87	0.07938615950217098
1106	88-89	0.14085816089058056
1106	90-91	0.12366990269875089
1106	92-93	0.11135312066107872
1106	94-95	0.14372218644025025
1106	96-97	0.09522507836052085
1106	98-99	0.11496087678515465
1106	100	0.17172733375215543
1107	1	0.18560991260951454
1107	2	0.21106544915052794
1107	3	0.17959007983253628
1107	4	0.16226792993590777
1107	5	0.10121801937193453
1107	6	0.14430108449342782
1107	7	0.06770966936329614
1107	8	0.18427357110459042
1107	9	0.16927644434208844
1107	10-11	0.16396783406506188
1107	12-13	0.1573278844931849
1107	14-15	0.16680987849920825
1107	16-17	0.18308693581709434
1107	18-19	0.180945214234427
1107	20-21	0.12475429127115234
1107	22-23	0.11391331068560362
1107	24-25	0.15939201143902437
1107	26-27	0.17690786940939063
1107	28-29	0.1556332148306936
1107	30-31	0.15912948947544692
1107	32-33	0.19525048325618144
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2216	28-29	-0.22339427655384014
2216	30-31	-0.20706616538912215
2216	32-33	-0.21498627426888106
2216	34-35	-0.24228853755890611
2216	36-37	-0.2587098879277434
2216	38-39	-0.20398930243885616
2216	40-41	-0.19553611835484475
2216	42-43	-0.21858831817615965
2216	44-45	-0.13895550416656732
2216	46-47	-0.07932725981936528
2216	48-49	-0.09399395335289285
2216	50-51	-0.08798158778149201
2216	52-53	-0.14054751678826705
2216	54-55	-0.11061671476785051
2216	56-57	-0.24064575434292124
2216	58-59	-0.213432959905127
2216	60-61	-0.16771641404327653
2216	62-63	-0.08396670940261686
2216	64-65	-0.032258458214037944
2216	66-67	-0.07875707129948495
2216	68-69	-0.10669131409027699
2216	70-71	-0.19251186651271013
2216	72-73	-0.08242121990075901
2216	74-75	-0.10593436563272007
2216	76-77	-0.23330807266367515
2216	78-79	-0.17241469069532656
2216	80-81	-0.15055982158884262
2216	82-83	-0.12678204911815527
2216	84-85	-0.1295114528665593
2216	86-87	-0.1729903762027547
2216	88-89	-0.15862679820158831
2216	90-91	-0.1691485852215422
2216	92-93	-0.12883401837372332
2216	94-95	-0.07704205101694583
2216	96-97	-0.10914655855249578
2216	98-99	-0.08493323752570348
2216	100	-0.044330278782005905
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	36303.0
3	6786.0
4	4423.0
5	3654.0
6	3140.0
7	3551.0
8	4136.0
9	4671.0
10	5034.0
11	5557.0
12	5991.0
13	6708.0
14	7392.0
15	7766.0
16	7839.0
17	8124.0
18	8105.0
19	7961.0
20	8246.0
21	8589.0
22	9850.0
23	12927.0
24	19341.0
25	27062.0
26	29064.0
27	26437.0
28	27205.0
29	34658.0
30	42874.0
31	52954.0
32	69663.0
33	94641.0
34	132152.0
35	202579.0
36	383118.0
37	824452.0
38	1362428.0
39	588373.0
40	1171.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.906924267644854	19.709752991382214	13.402161625536129	23.9811611154368
2	15.232061073181608	20.039270971168037	40.802886228118815	23.925781727531536
3	18.0937912213317	25.488375798279574	29.96240687858331	26.45542610180542
4	12.080736290263278	16.60659662172875	39.18058807161108	32.13207901639689
5	12.749671217622296	38.297045319563274	35.930838308943294	13.022445153871129
6	30.952852549581817	38.233213131289695	17.92978132834452	12.884152990783967
7	27.353152214103698	32.50780171220047	22.787862299108046	17.35118377458779
8	24.907145004734645	36.937664604362126	20.41454348461649	17.740646906286734
9	25.056671812987535	16.98578985278396	21.046058716907652	36.91147961732085
10-11	23.866507215717252	26.944059536170673	29.10442693773328	20.085006310378798
12-13	24.38381756623082	24.29156605789301	29.075858241891904	22.248758133984264
14-15	21.749376763110384	26.21885204729334	25.962070999117152	26.06970019047912
16-17	21.57498235877309	29.585577109320404	26.62563692217807	22.21380360972843
18-19	21.55337768803383	28.766532563676122	28.289445562987087	21.39064418530296
20-21	22.625841232550183	27.96054684527794	27.851042955544912	21.562568966626962
22-23	22.308989900877236	27.72050866742553	27.841128563565658	22.12937286813157
24-25	22.222864100970106	27.842745949141218	27.76375008091336	22.170639868975314
26-27	21.933565064552475	27.907511545241448	27.825857677039046	22.333065713167034
28-29	21.938200271217706	27.98991628986685	27.891568119023148	22.180315319892294
30-31	22.143356555817924	28.2401411301772	27.64767772100899	21.968824592995883
32-33	21.9203924294838	28.00491074242003	27.988666991378842	22.086029836717323
34-35	22.09377174913779	27.82735585561436	27.99473605988269	22.084136335365155
36-37	22.35407770603941	28.0226464327338	27.594414273949397	22.028861587277397
38-39	22.079438581819733	28.20614430316152	27.731379777305065	21.983037337713686
40-41	21.943232014294267	27.936197958802627	28.0053271351128	22.11524289179031
42-43	21.945523353213854	27.83515427299771	27.967159220557797	22.252163153230633
44-45	21.9538101170512	28.02336676734563	27.94415138429706	22.07867173130611
46-47	22.06811148384084	28.120547071344426	27.833471504248376	21.977869940566357
48-49	22.066009579609347	28.014166470683477	27.72538127582735	22.194442673879824
50-51	21.99922874763807	27.97495701086225	27.752654141183143	22.273160100316534
52-53	22.018019034514726	27.909648148806195	27.90818280939512	22.164150007283958
54-55	22.091083747824364	28.25792148591097	27.729883478505933	21.921111287758734
56-57	22.012993316519314	27.98332518274713	27.725864343719074	22.27781715701448
58-59	21.858994162100913	27.707748495345918	28.103739015017847	22.32951832753532
60-61	22.123931301965037	28.0250212436112	27.699874021936083	22.151173432487685
62-63	21.917999742111444	28.05717604215306	27.747709773780937	22.27711444195456
64-65	21.960983177907423	27.88659877525857	27.810637015246197	22.34178103158781
66-67	21.76621380448193	27.88228318343751	27.775136244506506	22.57636676757405
68-69	22.152255084797606	28.049715497501147	27.652099459812185	22.145929957889063
70-71	22.078018493590935	27.847485020113194	27.756736309057484	22.317760177238384
72-73	21.929520906222226	27.891198825450193	27.861502955361807	22.317777312965774
74-75	22.024374697788915	27.931508588900122	27.74789856452787	22.296218148783097
76-77	21.996059605337788	27.922293512391118	27.659433655268582	22.422213227002512
78-79	22.03708488490679	27.984943225110676	27.77417426335265	22.203797626629886
80-81	21.99492791343678	27.840172493436437	27.838108882696428	22.326790710430355
82-83	21.915117044359757	27.8223558329253	27.98440222574158	22.278124896973367
84-85	21.914897747840648	27.7876337655763	27.810260003929372	22.487208482653685
86-87	22.04580873371926	27.83685429714368	27.792029486934805	22.325307482202255
88-89	21.87699418256065	27.805871291104502	27.83815286504496	22.478981661289886
90-91	21.881312160305807	27.672830189328263	27.91617045710575	22.52968719326018
92-93	21.930179406874448	27.8046074933342	27.782409662382907	22.482803437408442
94-95	21.812064627211456	27.62803217947953	27.99969195191207	22.560211241396946
96-97	21.924681998973437	27.617791771000693	27.924582020086913	22.532944209938957
98-99	21.853462537942534	27.695280153890028	27.859295561561787	22.59196174660565
100	22.005210636261626	27.567611518227892	27.808246030857024	22.618931814653454
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	336.0
1	327.5
2	293.5
3	266.0
4	241.0
5	200.0
6	183.0
7	191.5
8	198.5
9	214.5
10	220.0
11	223.5
12	261.0
13	292.0
14	319.5
15	397.0
16	495.5
17	677.5
18	964.5
19	1336.0
20	1953.5
21	2902.0
22	4517.0
23	7400.0
24	10911.0
25	15059.0
26	21024.0
27	28839.0
28	39197.0
29	51011.5
30	61502.5
31	72388.0
32	84595.0
33	96996.0
34	108699.0
35	118665.5
36	127581.5
37	136763.0
38	143814.5
39	147717.0
40	151621.5
41	154264.5
42	153432.0
43	152579.0
44	151262.5
45	147655.5
46	145674.5
47	142542.0
48	137883.0
49	134832.0
50	131859.5
51	127669.5
52	118736.0
53	110534.5
54	105385.0
55	99402.5
56	91703.5
57	82465.0
58	73945.5
59	66890.0
60	60533.0
61	52543.5
62	44758.0
63	37712.5
64	31210.0
65	25522.0
66	19939.5
67	14673.5
68	10767.0
69	8148.5
70	5760.0
71	3997.5
72	2928.0
73	2127.0
74	1568.0
75	1124.0
76	732.0
77	496.5
78	331.5
79	213.0
80	142.5
81	87.5
82	61.0
83	46.0
84	33.0
85	28.5
86	23.5
87	13.5
88	8.0
89	6.0
90	6.0
91	5.5
92	4.5
93	2.5
94	4.0
95	4.5
96	3.5
97	4.0
98	3.0
99	1.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3264284449654145
2	0.11919632227696479
3	0.06561780740795009
4	0.04024493733096455
5	0.043761485253087665
6	0.010500802823006526
7	0.007594766692918673
8	0.015580260932739917
9	0.018022308100880968
10-11	0.02139233319291562
12-13	0.01667918215840339
14-15	0.018938075788933865
16-17	0.020073627722119455
18-19	0.019731741118579706
20-21	0.024579204747339694
22-23	0.01608088060220883
24-25	0.025397290548666948
26-27	0.01987826394866817
28-29	0.01946311593008419
30-31	0.020330042674774264
32-33	0.02547055196371118
34-35	0.01595877824380178
36-37	0.01018333669114819
38-39	0.014151663339377401
40-41	0.010171126455307484
42-43	0.009316409946458116
44-45	0.009572824899112925
46-47	0.008754739097785673
48-49	0.004542207732742358
50-51	0.006984254900883411
52-53	0.007582556457077968
54-55	0.003516547922123116
56-57	0.003113610139379842
58-59	0.0031746613185833685
60-61	0.004017167591592032
62-63	0.0032479227336276004
64-65	0.002478677875663168
66-67	0.0019780582061942526
68-69	0.0035531786296452315
70-71	0.002466467639822463
72-73	0.001941427498672137
74-75	0.0025641495265481054
76-77	0.003846224289822158
78-79	0.003834014053981453
80-81	0.0039194857048663895
82-83	0.002686251884955158
84-85	0.0029670873092913787
86-87	0.002307734573893295
88-89	0.5980939821852659
90-91	0.6081674267538478
92-93	0.6033688040684506
94-95	0.5893148226157988
96-97	0.5876908612489851
98-99	0.5852610243166847
100	0.5866041502591622
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4094925.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.351232675121096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.5627822036286	39.610882086320615
2	15.559772174677388	17.225051400248862
3	5.49724787346177	9.128383383503913
4	2.6218526675930844	5.8049110817532705
5	1.4214324536778091	3.933901923774435
6	0.8231219781367486	2.7336489679111864
7	0.5298229764293776	2.0528448391477374
8	0.3886982724939464	1.7211942816984036
9	0.2750516822350725	1.3702004694969274
>10	1.229744269632754	11.748791554704937
>50	0.06658035957817238	2.5065513063766915
>100	0.023672257723787362	2.0954329542671295
>500	2.2083073139161747E-4	0.06820575079590101
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.4420471681410527E-5	0.0	2.4420471681410527E-5	0.0
6	0.0	2.4420471681410527E-5	0.0	2.4420471681410527E-5	0.0
7	0.0	2.4420471681410527E-5	0.0	2.4420471681410527E-5	0.0
8	0.0	2.4420471681410527E-5	0.0	2.4420471681410527E-5	0.0
9	0.0	2.4420471681410527E-5	0.0	2.4420471681410527E-5	0.0
10-11	0.0	3.663070752211579E-5	0.0	3.663070752211579E-5	0.0
12-13	0.0	4.884094336282105E-5	0.0	4.884094336282105E-5	0.0
14-15	0.0	4.884094336282105E-5	0.0	4.884094336282105E-5	0.0
16-17	0.0	4.884094336282105E-5	0.0	4.884094336282105E-5	0.0
18-19	0.0	4.884094336282105E-5	0.0	4.884094336282105E-5	0.0
20-21	0.0	4.884094336282105E-5	0.0	6.105117920352632E-5	0.0
22-23	0.0	4.884094336282105E-5	0.0	1.2210235840705264E-4	0.0
24-25	0.0	6.105117920352632E-5	0.0	2.5641495265481056E-4	0.0
26-27	0.0	7.326141504423158E-5	0.0	4.5177872610609475E-4	0.0
28-29	0.0	7.326141504423158E-5	0.0	0.001245444055751937	0.0
30-31	0.0	7.326141504423158E-5	0.0	0.004700940798671526	0.0
32-33	0.0	7.326141504423158E-5	0.0	0.012906219283625463	0.0
34-35	0.0	7.326141504423158E-5	0.0	0.024139636257074305	0.0
36-37	0.0	7.326141504423158E-5	0.0	0.04346843959291073	0.0
38-39	0.0	7.326141504423158E-5	0.0	0.07852402669157554	0.0
40-41	0.0	7.326141504423158E-5	0.0	0.12006324902165486	0.0
42-43	0.0	7.326141504423158E-5	0.0	0.1612239540406723	0.0
44-45	0.0	7.326141504423158E-5	0.0	0.21080972178977636	0.0
46-47	0.0	7.326141504423158E-5	0.0	0.2725690946720636	0.0
48-49	0.0	7.326141504423158E-5	0.0	0.34056789806895116	0.0
50-51	0.0	7.326141504423158E-5	0.0	0.4154044335366338	0.0
52-53	1.2210235840705263E-5	7.326141504423158E-5	0.0	0.4920847146162628	0.0
54-55	2.4420471681410527E-5	7.326141504423158E-5	0.0	0.574393914418457	0.0
56-57	2.4420471681410527E-5	7.326141504423158E-5	0.0	0.6731991428414439	0.0
58-59	2.4420471681410527E-5	7.326141504423158E-5	0.0	0.7857408865852245	0.0
60-61	4.884094336282105E-5	7.326141504423158E-5	0.0	0.9058285560785606	0.0
62-63	4.884094336282105E-5	7.326141504423158E-5	0.0	1.0310323143891524	0.0
64-65	4.884094336282105E-5	7.326141504423158E-5	0.0	1.1624755032143446	0.0
66-67	4.884094336282105E-5	7.326141504423158E-5	0.0	1.3128567678284706	0.0
68-69	4.884094336282105E-5	8.547165088493685E-5	0.0	1.484093115258521	0.0
70-71	4.884094336282105E-5	9.76818867256421E-5	0.0	1.6648534466443219	0.0
72-73	4.884094336282105E-5	1.2210235840705264E-4	0.0	1.855418597410209	0.0
74-75	4.884094336282105E-5	1.5873306592916841E-4	0.0	2.049964285060166	0.0
76-77	4.884094336282105E-5	1.953637734512842E-4	0.0	2.2602001257654294	0.0
78-79	4.884094336282105E-5	1.953637734512842E-4	0.0	2.502683199325995	0.0
80-81	4.884094336282105E-5	1.953637734512842E-4	0.0	2.7684267721631044	0.0
82-83	4.884094336282105E-5	2.0757400929198946E-4	0.0	3.0447444092382643	0.0
84-85	4.884094336282105E-5	2.1978424513269474E-4	0.0	3.316947196835107	0.0
86-87	4.884094336282105E-5	2.1978424513269474E-4	0.0	3.609174771210706	0.0
88	4.884094336282105E-5	2.1978424513269474E-4	0.0	3.8472255291610957	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5040	0.0	36.17008	1
TCAACGC	5100	0.0	33.897877	4
CAACGCA	5390	0.0	32.07406	5
GGTATCA	3225	0.0	31.98773	1
ATCAACG	5440	0.0	31.958218	3
AACGCAG	5865	0.0	29.465254	6
GTACATG	12120	0.0	28.721748	1
TACATGG	12180	0.0	27.212666	2
TATCAAC	6645	0.0	27.168488	2
ACATGGG	12600	0.0	25.32089	3
ACGCAGA	6980	0.0	24.489298	7
CGCAGAG	7130	0.0	23.908232	8
CATGGGG	8495	0.0	21.34611	4
GAGTACA	9000	0.0	19.522497	1
GCAGAGT	8900	0.0	18.520277	9
AGTACAT	9130	0.0	17.919987	2
TCTATAC	1835	0.0	17.412157	3
CATGGGA	4100	0.0	17.301664	4
TATACCG	275	6.6408757E-7	17.082922	5
TAGCGCG	175	0.00233726	16.106754	4
>>END_MODULE