FastQCFastQC Report
Wed 25 May 2016
SRR1033162_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033162_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4094925
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56730.13853733584864192No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47950.11709616171236348No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54750.049.417721
GTATCAA97600.042.184921
TCAACGC113300.035.6443524
ATCAACG114450.035.3272743
CAACGCA115300.035.0668375
AACGCAG120050.033.587496
TATCAAC124350.032.85542
ACGCAGA140100.028.4788237
CGCAGAG143700.027.6672538
GTACATG119450.027.6140981
TACATGG120150.026.490962
ACATGGG126450.024.7990173
GCAGAGT162700.023.9452539
CATGGGG83100.022.0643464
GTGGTAT29900.021.0878661
CAGAGTA156450.020.20082910-11
GAGTACT104700.019.99623112-13
AGAGTAC147600.019.80416310-11
GAGTACA88900.019.3192421
TGGTATC31750.018.0654532
Produced by FastQC (version 0.11.5)