FastQCFastQC Report
Wed 25 May 2016
SRR1033161_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033161_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3804217
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37050.032.9287031
TCAACGC35900.032.4529084
ATCAACG37700.031.0313053
CAACGCA38050.030.8656945
GTACATG97200.030.143051
TACATGG97700.028.6781352
AACGCAG41800.028.1986436
ACATGGG98650.027.1468733
GGTATCA23450.026.113411
TATCAAC47050.025.378932
ACGCAGA49050.023.0732547
GAGTACA67650.022.3511731
CATGGGG69200.021.520364
CGCAGAG52400.021.4191938
GTATAGA16350.020.4551981
AGTACAT70300.019.8609642
TATACCG2859.64812E-716.4833625
GTCTAGG11650.016.1732441
ACACCGT6100.016.1666586
GCAGAGT67550.016.0591729
Produced by FastQC (version 0.11.5)