##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033161_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3804217
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.651563514909903	33.0	31.0	34.0	30.0	34.0
2	31.8187669105101	34.0	31.0	34.0	30.0	34.0
3	31.91293530311231	34.0	31.0	34.0	30.0	34.0
4	35.31365639762401	37.0	35.0	37.0	33.0	37.0
5	35.39326252945087	37.0	35.0	37.0	33.0	37.0
6	35.4752715210515	37.0	37.0	37.0	35.0	37.0
7	35.399874665404205	37.0	37.0	37.0	33.0	37.0
8	35.390461164544504	37.0	36.0	37.0	33.0	37.0
9	37.09245818521919	39.0	38.0	39.0	35.0	39.0
10-11	37.077025574513755	39.0	38.0	39.0	34.5	39.0
12-13	37.02704801014243	39.0	38.0	39.0	34.0	39.0
14-15	38.48987465751822	41.0	39.0	41.0	34.5	41.0
16-17	38.49192435657588	41.0	39.0	41.0	34.5	41.0
18-19	38.472413771349004	41.0	39.0	41.0	34.0	41.0
20-21	38.48925902491892	41.0	39.0	41.0	34.0	41.0
22-23	38.44336180086467	41.0	39.0	41.0	34.0	41.0
24-25	38.42230989977701	41.0	39.0	41.0	34.0	41.0
26-27	38.36809795550569	40.5	39.0	41.0	34.0	41.0
28-29	38.330712732738434	40.0	39.0	41.0	34.0	41.0
30-31	38.32542662524246	40.0	39.0	41.0	34.0	41.0
32-33	38.39236865299745	41.0	39.0	41.0	34.0	41.0
34-35	38.365541056149006	41.0	39.0	41.0	34.0	41.0
36-37	38.289039242503776	41.0	39.0	41.0	34.0	41.0
38-39	38.21227535127465	41.0	39.0	41.0	33.0	41.0
40-41	38.150417286921325	41.0	38.0	41.0	33.0	41.0
42-43	38.04008498989411	40.5	38.0	41.0	33.0	41.0
44-45	37.91721305593241	40.0	38.0	41.0	33.0	41.0
46-47	37.79068767633392	40.0	38.0	41.0	33.0	41.0
48-49	37.66767392606678	40.0	38.0	41.0	32.5	41.0
50-51	37.533839026532924	40.0	37.5	41.0	32.0	41.0
52-53	37.376997947277985	40.0	37.0	41.0	32.0	41.0
54-55	37.21455085238303	40.0	37.0	41.0	31.5	41.0
56-57	37.04505565797114	40.0	36.5	41.0	31.0	41.0
58-59	36.83304803590332	40.0	36.0	41.0	31.0	41.0
60-61	36.59547667759226	39.0	35.0	41.0	31.0	41.0
62-63	36.32220664594054	39.0	35.0	41.0	30.5	41.0
64-65	36.03680586570114	39.0	35.0	41.0	30.0	41.0
66-67	35.73308410114355	38.0	35.0	40.0	30.0	41.0
68-69	35.4174886711247	37.0	35.0	40.0	30.0	41.0
70-71	35.06214314272819	37.0	35.0	39.5	29.0	41.0
72-73	34.68839724442638	36.0	34.0	39.0	29.0	41.0
74-75	34.31681473480614	36.0	34.0	39.0	29.0	40.5
76-77	33.98809531632922	35.0	34.0	38.5	29.0	40.0
78-79	33.62092330695121	35.0	34.0	37.0	28.5	39.0
80-81	33.26682139846386	35.0	34.0	37.0	28.0	39.0
82-83	33.05355806464247	35.0	34.0	36.0	28.0	38.5
84-85	32.79422716948061	35.0	34.0	36.0	28.0	37.0
86-87	32.548802158236505	35.0	34.0	36.0	27.0	37.0
88-89	32.19906632560655	35.0	34.0	35.0	26.5	36.5
90-91	32.016665847400404	35.0	34.0	35.0	26.0	36.0
92-93	31.84301973310145	35.0	33.0	35.0	26.0	36.0
94-95	31.67649545228361	35.0	33.0	35.0	25.5	36.0
96-97	31.505023372746614	35.0	33.0	35.0	25.0	36.0
98-99	31.31301290120937	35.0	33.0	35.0	24.5	35.0
100	31.150658335210636	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.20037040428224984
1101	2	0.2116220900803043
1101	3	0.1879226230535096
1101	4	0.2429653643824352
1101	5	0.22344929877215236
1101	6	0.17740448344083148
1101	7	0.2298647461215566
1101	8	0.15750215131265577
1101	9	0.2569718642759824
1101	10-11	0.23325410423534976
1101	12-13	0.19970879948274245
1101	14-15	0.2732971459624878
1101	16-17	0.20846388445799136
1101	18-19	0.18927678318306818
1101	20-21	0.1741337557261673
1101	22-23	0.2120095640518045
1101	24-25	0.20838387646627154
1101	26-27	0.2378019747646576
1101	28-29	0.2820383762795302
1101	30-31	0.2873311138625567
1101	32-33	0.276506366856367
1101	34-35	0.3151125466595275
1101	36-37	0.31861266909182007
1101	38-39	0.3238613024450103
1101	40-41	0.27118638150737695
1101	42-43	0.21872173868790412
1101	44-45	0.23195492632632408
1101	46-47	0.24407758580188244
1101	48-49	0.1838958749912507
1101	50-51	0.21142592905195556
1101	52-53	0.24112013093004236
1101	54-55	0.2193388622700283
1101	56-57	0.2361757036107619
1101	58-59	0.18056854598087568
1101	60-61	0.17296145838386678
1101	62-63	0.2567527234394831
1101	64-65	0.23596069436151623
1101	66-67	0.19192012216390708
1101	68-69	0.25233942792373654
1101	70-71	0.24951556135297892
1101	72-73	0.23554193859887818
1101	74-75	0.24521347721087494
1101	76-77	0.23080878129025706
1101	78-79	0.3065036694705796
1101	80-81	0.28693530065123696
1101	82-83	0.2671418217425554
1101	84-85	0.2400563752857039
1101	86-87	0.2845780422249362
1101	88-89	0.4489327662600431
1101	90-91	0.4814571455276564
1101	92-93	0.47214108083918305
1101	94-95	0.43081876785629447
1101	96-97	0.49318831222698023
1101	98-99	0.549510844486111
1101	100	0.5216414397505247
1102	1	0.08770027074347198
1102	2	0.14042280913526284
1102	3	0.0320385706652182
1102	4	0.07437907778766828
1102	5	3.5928849993638323E-4
1102	6	0.02064408282449648
1102	7	0.024709378889923528
1102	8	0.06947891042924681
1102	9	0.07144926540593133
1102	10-11	0.051017008703766464
1102	12-13	0.05040698900508289
1102	14-15	0.12114800626037692
1102	16-17	0.08681519416523287
1102	18-19	0.09849930501152215
1102	20-21	0.044049009963451624
1102	22-23	0.062325755628585
1102	24-25	0.05436326783761558
1102	26-27	0.07956815350117807
1102	28-29	0.12786528947932396
1102	30-31	0.1302882273705066
1102	32-33	0.12846450758570427
1102	34-35	0.17516968584802584
1102	36-37	0.1360770373506739
1102	38-39	0.11815861882918455
1102	40-41	0.17508982269895768
1102	42-43	0.17946904223181548
1102	44-45	0.18521153906386445
1102	46-47	0.1504319129718894
1102	48-49	0.17231395408730066
1102	50-51	0.1535404000329521
1102	52-53	0.10785260396547613
1102	54-55	0.1504026783974055
1102	56-57	0.06297815610177793
1102	58-59	0.03741080329982083
1102	60-61	-0.016390750142072363
1102	62-63	0.0947133680208907
1102	64-65	0.08807291829577224
1102	66-67	-0.0029213005376860224
1102	68-69	-0.01238835841422059
1102	70-71	0.04663613146083634
1102	72-73	0.07156530788495985
1102	74-75	-0.03442988437104333
1102	76-77	0.008034962594827277
1102	78-79	0.050009704396103416
1102	80-81	0.0681596201170791
1102	82-83	-0.036700628180405204
1102	84-85	-0.04101065090844003
1102	86-87	0.044883961588055854
1102	88-89	0.1141067518789427
1102	90-91	0.13646966478805211
1102	92-93	0.20042979424442464
1102	94-95	0.1873127329307671
1102	96-97	0.21771803744526963
1102	98-99	0.20435644027450195
1102	100	0.23412217934990665
1103	1	0.08793602107077447
1103	2	0.09758880433667372
1103	3	0.06164860808746653
1103	4	0.06690116028518389
1103	5	0.09894875492386745
1103	6	0.09437454992831817
1103	7	0.08181869287157184
1103	8	0.03777981536212849
1103	9	0.13730406315160337
1103	10-11	0.13397013186004614
1103	12-13	0.13421981244624703
1103	14-15	0.15281617950964943
1103	16-17	0.09753553029118933
1103	18-19	0.13165722155878967
1103	20-21	0.11939668287278238
1103	22-23	0.15146510363301502
1103	24-25	0.1637490140911737
1103	26-27	0.21553849805556524
1103	28-29	0.17350055460977387
1103	30-31	0.1640800675224483
1103	32-33	0.1544861040435137
1103	34-35	0.12361629031284593
1103	36-37	0.18526553717367733
1103	38-39	0.16058853288725317
1103	40-41	0.16397594893442857
1103	42-43	0.11731271163491641
1103	44-45	0.10954177705475843
1103	46-47	0.12651403612255052
1103	48-49	0.17695366662150036
1103	50-51	0.0919744636901072
1103	52-53	0.00461476830308527
1103	54-55	0.0645009553445135
1103	56-57	0.0963267132943244
1103	58-59	0.08956579588280533
1103	60-61	0.08266719674755052
1103	62-63	0.11892859300389347
1103	64-65	0.07273913825542877
1103	66-67	0.08747853735543032
1103	68-69	0.02886096028735352
1103	70-71	0.05300581522598691
1103	72-73	0.06896843708161526
1103	74-75	0.07096106503587407
1103	76-77	0.040847892796151086
1103	78-79	0.029049277299250775
1103	80-81	0.1053873465750641
1103	82-83	0.07879494267744036
1103	84-85	0.05610092470304551
1103	86-87	0.07855648671883841
1103	88-89	0.18605379743182482
1103	90-91	0.23211179558049366
1103	92-93	0.17251076410434152
1103	94-95	0.1547262642199243
1103	96-97	0.22137532818129557
1103	98-99	0.21732736147426124
1103	100	0.2836362801202661
1104	1	0.10889164345602609
1104	2	0.14984855608124903
1104	3	0.15536509685534838
1104	4	0.11990578304859412
1104	5	0.10166092466733545
1104	6	0.07439782368697934
1104	7	0.1542280545837187
1104	8	0.10379948394639626
1104	9	0.17210279203759882
1104	10-11	0.1617203215134566
1104	12-13	0.1943324313671866
1104	14-15	0.14591358909901686
1104	16-17	0.20493875301329467
1104	18-19	0.16184386093230074
1104	20-21	0.1748369214583292
1104	22-23	0.153737358843685
1104	24-25	0.11840903105532163
1104	26-27	0.17794067390216384
1104	28-29	0.19639318854554233
1104	30-31	0.16955133878689566
1104	32-33	0.15742307600055483
1104	34-35	0.13098611375362879
1104	36-37	0.1729045508831888
1104	38-39	0.09221415326148019
1104	40-41	0.07050225789821951
1104	42-43	0.1222743211407149
1104	44-45	0.1048351735468529
1104	46-47	0.037391174366185
1104	48-49	-0.03036761061531479
1104	50-51	0.014375971751206862
1104	52-53	0.01454859449680157
1104	54-55	0.024595735520172468
1104	56-57	0.08537953429274836
1104	58-59	0.0049414967698737655
1104	60-61	0.09511342408808332
1104	62-63	-0.006255218955182329
1104	64-65	0.006760263959648682
1104	66-67	0.06519091307169589
1104	68-69	0.011906397019224357
1104	70-71	0.030494923100931715
1104	72-73	0.14350577297842193
1104	74-75	0.08698476468291005
1104	76-77	-0.019643382513031327
1104	78-79	-0.01515376925023304
1104	80-81	0.05850707060687199
1104	82-83	0.08508396764291604
1104	84-85	0.08512959668350817
1104	86-87	0.14662484246918694
1104	88-89	0.2462179679249914
1104	90-91	0.12379810638154254
1104	92-93	0.12209189215281313
1104	94-95	0.13945820955344246
1104	96-97	0.23204692058317633
1104	98-99	0.271683532704472
1104	100	0.33548892287801024
1105	1	0.2660546717952421
1105	2	0.29442729208711427
1105	3	0.2557704664365872
1105	4	0.21111611386681517
1105	5	0.1771150215056707
1105	6	0.17629680492458277
1105	7	0.18843680248576788
1105	8	0.18785604953006896
1105	9	0.1960122853636861
1105	10-11	0.22889873313154396
1105	12-13	0.28218920483335097
1105	14-15	0.26903355628409287
1105	16-17	0.23242815162029729
1105	18-19	0.26306000805906393
1105	20-21	0.29152411664908584
1105	22-23	0.3468319574045253
1105	24-25	0.35108632163303355
1105	26-27	0.3545560301337858
1105	28-29	0.41501829176390714
1105	30-31	0.3742141509017003
1105	32-33	0.3668707966498843
1105	34-35	0.3670489155561967
1105	36-37	0.37720798588294
1105	38-39	0.280541291573698
1105	40-41	0.3074667570495748
1105	42-43	0.2665406832508239
1105	44-45	0.2424056799751142
1105	46-47	0.25823356929328156
1105	48-49	0.26464983063370084
1105	50-51	0.27586046232011086
1105	52-53	0.3008198385445553
1105	54-55	0.32000185293078687
1105	56-57	0.29341279229299033
1105	58-59	0.23058736802244084
1105	60-61	0.23756119821777588
1105	62-63	0.22231975730491627
1105	64-65	0.1919567063900871
1105	66-67	0.20012058704367774
1105	68-69	0.08662614652133271
1105	70-71	0.10815737160049821
1105	72-73	0.0928415482401519
1105	74-75	0.07682127408406103
1105	76-77	0.13390636330175454
1105	78-79	0.21030783147054422
1105	80-81	0.14321969813769186
1105	82-83	0.19498258014753844
1105	84-85	0.1440662920632434
1105	86-87	0.20798945643697664
1105	88-89	0.15578823599463476
1105	90-91	0.2368588248811676
1105	92-93	0.2951185469923878
1105	94-95	0.2958462365300605
1105	96-97	0.3234193877189888
1105	98-99	0.2903548938716405
1105	100	0.3388616077735449
1106	1	0.036983109592210184
1106	2	0.08064088952070847
1106	3	0.0327208643129957
1106	4	0.00866809769384247
1106	5	-0.0013547662737423138
1106	6	0.021954783978294756
1106	7	-0.05875866979779687
1106	8	0.0036276678782130034
1106	9	-0.04799253756097954
1106	10-11	-0.0913450269244862
1106	12-13	-0.028630905631658266
1106	14-15	-0.007752795692198333
1106	16-17	-0.03838608642307406
1106	18-19	-0.006991534344379602
1106	20-21	0.05140605319915892
1106	22-23	0.013915000588646365
1106	24-25	0.11558783040893417
1106	26-27	0.11160901187571426
1106	28-29	0.15036116877436712
1106	30-31	0.10798253445812378
1106	32-33	0.13982655906281138
1106	34-35	0.1489596249883789
1106	36-37	0.14363179518001346
1106	38-39	0.14377078124835663
1106	40-41	0.07735462553907269
1106	42-43	0.08152939471057863
1106	44-45	0.1374759456823611
1106	46-47	0.1319937393951136
1106	48-49	0.12928270012508136
1106	50-51	0.13743200964370317
1106	52-53	0.11403801983970396
1106	54-55	0.15954351141376577
1106	56-57	0.13174202385884115
1106	58-59	0.13679626355087038
1106	60-61	0.13886235623550647
1106	62-63	0.1854865549107103
1106	64-65	0.19471200793695687
1106	66-67	0.19620146477231515
1106	68-69	0.12502764260010935
1106	70-71	0.13022383908097623
1106	72-73	0.1809478360959389
1106	74-75	0.19836973842596706
1106	76-77	0.13135379421647997
1106	78-79	0.09039499030963327
1106	80-81	0.12015419980517805
1106	82-83	0.07398229942197787
1106	84-85	0.12833288909409646
1106	86-87	0.14390727526880198
1106	88-89	0.2152660963698807
1106	90-91	0.2293127134757711
1106	92-93	0.18944930419751316
1106	94-95	0.18592357989412278
1106	96-97	0.2420258382567333
1106	98-99	0.2548529059775575
1106	100	0.31388000868932764
1107	1	0.24635609092363353
1107	2	0.2876661076536777
1107	3	0.2595024482353594
1107	4	0.2512952643831454
1107	5	0.23293455819847253
1107	6	0.2260565927771907
1107	7	0.26315937342569384
1107	8	0.2593703870189472
1107	9	0.3010273101501255
1107	10-11	0.2845520792703269
1107	12-13	0.24790939911858345
1107	14-15	0.21779868031156013
1107	16-17	0.2616132129933959
1107	18-19	0.30916088604598
1107	20-21	0.2398844899565873
1107	22-23	0.24559590886106264
1107	24-25	0.2524579609042945
1107	26-27	0.29014738085977143
1107	28-29	0.2886151762918132
1107	30-31	0.35545800864539245
1107	32-33	0.3363608980748296
1107	34-35	0.3495872580148216
1107	36-37	0.31923849153704964
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2216	12-13	-0.09859827771825991
2216	14-15	-0.18373887762817986
2216	16-17	-0.14137472666787687
2216	18-19	-0.1945763595829888
2216	20-21	-0.21335893860463528
2216	22-23	-0.2337524836608651
2216	24-25	-0.18734097874865085
2216	26-27	-0.3275140286357967
2216	28-29	-0.23396436002565935
2216	30-31	-0.20352019584468906
2216	32-33	-0.1691062467135822
2216	34-35	-0.23524011791727872
2216	36-37	-0.2533514932800074
2216	38-39	-0.18169764766390983
2216	40-41	-0.18756315358640308
2216	42-43	-0.23025812423117742
2216	44-45	-0.17636556673993198
2216	46-47	-0.10705280046877164
2216	48-49	-0.09534008484085632
2216	50-51	-0.1243326650716341
2216	52-53	-0.09820827882501959
2216	54-55	-0.13824707896582566
2216	56-57	-0.1659847820169844
2216	58-59	-0.21279192902045452
2216	60-61	-0.06926464543633415
2216	62-63	-0.04943783497930099
2216	64-65	-0.060862003688534116
2216	66-67	-0.07092457259195584
2216	68-69	-0.01959750422652462
2216	70-71	-0.10007067210964493
2216	72-73	-0.012565228926753491
2216	74-75	-0.027062172329529233
2216	76-77	-0.05086793620616703
2216	78-79	-0.061612397688449505
2216	80-81	-0.06490904874571157
2216	82-83	-0.04735727501107334
2216	84-85	-0.09546976572698895
2216	86-87	-0.1503848981076743
2216	88-89	-0.06211207726580881
2216	90-91	-0.109428700070346
2216	92-93	-0.08331891065967412
2216	94-95	-0.06554168057896703
2216	96-97	-0.08152543837075044
2216	98-99	-0.12227200445921582
2216	100	-0.0929180948119317
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	38968.0
3	7010.0
4	4591.0
5	3712.0
6	3182.0
7	3624.0
8	4155.0
9	4620.0
10	5075.0
11	5718.0
12	6287.0
13	6880.0
14	7362.0
15	7844.0
16	7762.0
17	7896.0
18	7803.0
19	8221.0
20	8106.0
21	8654.0
22	9925.0
23	12596.0
24	18341.0
25	24315.0
26	26222.0
27	24828.0
28	26466.0
29	33429.0
30	41204.0
31	50740.0
32	66231.0
33	90158.0
34	125640.0
35	190330.0
36	361611.0
37	779621.0
38	1246900.0
39	516999.0
40	1191.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.58756463155563	18.637413181738463	11.875195328725884	24.899826857980027
2	16.015294795095645	19.774278342321704	39.391124197441705	24.819302665140942
3	18.66470686332739	24.441611240320885	29.242278934235937	27.65140296211579
4	12.307727910076146	15.61736394609702	38.343601519347914	33.73130662447892
5	13.04114916387819	37.804834432418616	35.676266174646734	13.477750229056456
6	32.34592502819527	37.28523619614967	17.280740727109254	13.088098048545808
7	28.41668292175109	31.866449978942875	22.012109555939137	17.7047575433669
8	25.840606663858818	36.22957275985889	19.9436747394983	17.986145836783994
9	25.53516775350878	16.002042314935586	20.281543945921772	38.18124598563386
10-11	24.408156257632953	26.359365317079853	28.746235944524535	20.486242480762662
12-13	24.894905580983927	23.621691903480478	28.69272520124666	22.79067731428894
14-15	22.03395512175525	25.698128377324934	25.44206099081081	26.825855510109005
16-17	22.091555954841592	29.036740406191157	26.028738866538596	22.84296477242865
18-19	21.992577325875953	28.26253108665457	27.74792354687719	21.99696804059229
20-21	22.922423989534373	27.706180027205264	27.262908070696867	22.108487912563497
22-23	22.56826601444469	27.333932237585685	27.45209620431942	22.64570554365021
24-25	22.55248979626179	27.460236118301573	27.371825193261678	22.615448892174957
26-27	22.266770641704944	27.508448190957907	27.418385861147577	22.80639530618957
28-29	22.327390073843894	27.55928656697857	27.500038451461702	22.613284907715837
30-31	22.460507534349762	28.063937791166804	27.122594320362552	22.352960354120878
32-33	22.168605987988997	27.67147214490923	27.659232656375245	22.500689210726524
34-35	22.291907089317142	27.46233435073824	27.687934543606552	22.55782401633807
36-37	22.561919616556203	27.832168677834495	27.207142881554258	22.39876882405504
38-39	22.37136815272625	27.989576975753444	27.32956077308522	22.30949409843509
40-41	22.166855642418092	27.659359399806355	27.64444026153578	22.52934469623977
42-43	22.186426918605783	27.637884140464923	27.651777841689096	22.5239110992402
44-45	22.256554252962644	27.776017872343555	27.6263149122498	22.341112962443997
46-47	22.263881259650468	27.946488554565036	27.501891810147832	22.287738375636668
48-49	22.272193869369612	27.807063847282603	27.465322448782164	22.455419834565618
50-51	22.23865253554567	27.668187587910392	27.49327704439431	22.59988283214963
52-53	22.266026519001215	27.689979321299063	27.57459816389119	22.469395995808526
54-55	22.333790867739435	28.110028021189326	27.39174763553553	22.16443347553571
56-57	22.2142951026524	27.785481454220445	27.453799847532924	22.546423595594227
58-59	22.035232503027654	27.505802290738334	27.83170057787635	22.62726462835766
60-61	22.377333948814794	27.887316945964162	27.408001118802293	22.32734798641875
62-63	22.180086364779868	27.863130198835623	27.473523940109835	22.483259496274673
64-65	22.163335852528505	27.60716327670621	27.64061380738015	22.58888706338514
66-67	21.997356037726277	27.64099839674628	27.543657053540894	22.81798851198655
68-69	22.413827549159627	27.833169956417926	27.39734921940534	22.355653275017108
70-71	22.326183985112994	27.557284556782207	27.555720464348816	22.560810993755986
72-73	22.059364349582758	27.657194079600224	27.69725574933592	22.5861858214811
74-75	22.133974392254718	27.740089863036744	27.546773270313984	22.579162474394554
76-77	22.098928221519635	27.700325258520458	27.494229178315432	22.70651734164447
78-79	22.256093834223105	27.768163076893554	27.56639236838539	22.40935072049795
80-81	22.170415130428083	27.504847111757552	27.712637742291513	22.612100015522852
82-83	22.005391522875197	27.62569267005247	27.820376328822434	22.5485394782499
84-85	21.993962564323716	27.59655145627801	27.602229542689287	22.807256436708986
86-87	22.131871517433567	27.705331604333928	27.57729960119628	22.58549727703623
88-89	21.975921214588592	27.565003636821373	27.694728612204567	22.764346536385467
90-91	21.931554442288526	27.50112501416599	27.714028077021187	22.8532924665243
92-93	22.06475885810763	27.683323561295538	27.573199703691714	22.678717876905118
94-95	21.85306401124138	27.401049569219687	27.805201941327567	22.940684478211367
96-97	21.848240086139423	27.4624144846231	27.758267544137993	22.931077885099487
98-99	21.872976793804277	27.51581579573968	27.73147462009501	22.879732790361036
100	22.009028897708188	27.34939475501057	27.641790792195476	22.99978555508577
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	285.0
1	299.0
2	309.5
3	278.0
4	239.5
5	202.5
6	177.5
7	204.0
8	221.5
9	202.5
10	198.5
11	206.0
12	225.5
13	263.0
14	297.5
15	347.0
16	438.0
17	598.5
18	872.0
19	1271.5
20	1891.0
21	2784.0
22	4153.0
23	6498.0
24	9599.5
25	13348.5
26	18574.0
27	25303.0
28	33651.5
29	43286.0
30	52331.0
31	62051.5
32	72486.5
33	82785.0
34	92797.5
35	101535.0
36	109957.5
37	118859.0
38	125792.0
39	129535.5
40	132765.0
41	135298.0
42	136307.0
43	136188.5
44	135503.5
45	134905.0
46	134857.5
47	132459.0
48	129212.5
49	127529.5
50	125146.0
51	121497.5
52	115600.5
53	110120.5
54	106025.0
55	101349.5
56	95059.0
57	87089.0
58	79572.0
59	72900.5
60	65096.0
61	55900.0
62	47327.0
63	39689.0
64	32574.5
65	26279.0
66	20243.5
67	14798.0
68	10807.5
69	8049.5
70	5871.0
71	4153.5
72	3013.5
73	2152.0
74	1537.5
75	1078.5
76	697.5
77	449.0
78	288.5
79	182.0
80	106.0
81	68.0
82	46.0
83	41.0
84	35.5
85	23.5
86	19.0
87	15.5
88	15.5
89	11.0
90	5.5
91	10.0
92	7.5
93	2.5
94	5.0
95	4.5
96	3.5
97	4.0
98	2.5
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32942389984588155
2	0.11810577577462064
3	0.06505938015628446
4	0.04029738576952892
5	0.04366207290488424
6	0.010172921260800842
7	0.007333966490344793
8	0.01564053785575323
9	0.018137766589024756
10-11	0.021187014305440514
12-13	0.01612684029328506
14-15	0.01908408484584344
16-17	0.020017259793539643
18-19	0.019346951028293074
20-21	0.023881392675549264
22-23	0.015877117419957905
24-25	0.025077433805695104
26-27	0.01945209750127293
28-29	0.018939508445496144
30-31	0.02024069604862183
32-33	0.02506429049657262
34-35	0.015390814982426082
36-37	0.009831195223616319
38-39	0.013734758032993385
40-41	0.009936341696596173
42-43	0.008806017112062746
44-45	0.00922660300398216
46-47	0.008424861147510775
48-49	0.004495011719888744
50-51	0.007163103471752532
52-53	0.007964845328223915
54-55	0.0034566902992126895
56-57	0.00307553433466072
58-59	0.0030492477164157563
60-61	0.003916706118499549
62-63	0.00315439418939561
64-65	0.0024183688785366342
66-67	0.0020503562231071466
68-69	0.0034172603718452444
70-71	0.002470942115026561
72-73	0.0021555026960870002
74-75	0.002484085424149043
76-77	0.004363578628663927
78-79	0.0038509895728871405
80-81	0.0038772761911321043
82-83	0.0025498019697614517
84-85	0.0031149642620281652
86-87	0.0023657956420467074
88-89	0.598165141473265
90-91	0.6086272155347605
92-93	0.6051179519990578
94-95	0.5907917450555528
96-97	0.5898980000352241
98-99	0.5866516026819711
100	0.5877687839573821
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3804217.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.24130593078024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14442222550697	48.7871400681657
2	13.653026580439409	17.26870461709286
3	4.213214693293241	7.993475981118491
4	1.8211612531021535	4.606904638268657
5	0.90288725004456	2.854988440053445
6	0.536799071501382	2.0368724582506603
7	0.36999306663638704	1.6379191303593523
8	0.24303936511341934	1.2296101473888283
9	0.18424577515386223	1.0486749089663236
>10	0.8780869387423963	9.73914041085335
>50	0.04308471549845817	1.8439439347763111
>100	0.009997455550815385	0.9377313490214817
>500	4.160941694367723E-5	0.014893915684596491
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	2.628661824496342E-5	0.0	0.0	0.0
3	0.0	2.628661824496342E-5	0.0	2.628661824496342E-5	0.0
4	0.0	2.628661824496342E-5	0.0	2.628661824496342E-5	0.0
5	0.0	5.257323648992684E-5	0.0	2.628661824496342E-5	0.0
6	0.0	5.257323648992684E-5	0.0	2.628661824496342E-5	0.0
7	0.0	5.257323648992684E-5	0.0	2.628661824496342E-5	0.0
8	0.0	5.257323648992684E-5	0.0	2.628661824496342E-5	0.0
9	0.0	5.257323648992684E-5	0.0	5.257323648992684E-5	0.0
10-11	0.0	1.0514647297985367E-4	0.0	7.885985473489026E-5	0.0
12-13	0.0	1.314330912248171E-4	0.0	1.0514647297985367E-4	0.0
14-15	0.0	1.7086301859226222E-4	0.0	1.5771970946978052E-4	0.0
16-17	0.0	2.3657956420467078E-4	0.0	1.5771970946978052E-4	0.0
18-19	0.0	2.628661824496342E-4	0.0	1.8400632771474392E-4	0.0
20-21	0.0	2.628661824496342E-4	0.0	2.1029294595970735E-4	0.0
22-23	0.0	2.628661824496342E-4	0.0	2.760094915721159E-4	0.0
24-25	0.0	3.1543941893956103E-4	0.0	3.4172603718452444E-4	0.0
26-27	0.0	3.942992736744513E-4	0.0	5.257323648992684E-4	0.0
28-29	0.0	4.205858919194147E-4	0.0	0.0012354710575132807	0.0
30-31	0.0	4.205858919194147E-4	0.0	0.004363578628663927	0.0
32-33	0.0	4.205858919194147E-4	0.0	0.010356927588515585	0.0
34-35	0.0	4.205858919194147E-4	0.0	0.01947838411951789	0.0
36-37	0.0	4.8630243753182325E-4	0.0	0.03563151103104791	0.0
38-39	0.0	5.651622922667135E-4	0.0	0.06370561931666885	0.0
40-41	0.0	6.045922196341587E-4	0.0	0.09636674248603588	0.0
42-43	0.0	6.834520743690489E-4	0.0	0.1294878814746898	0.0
44-45	0.0	6.834520743690489E-4	0.0	0.16736689836568208	0.0
46-47	0.0	7.097386926140123E-4	0.0	0.2167725973570908	0.0
48-49	0.0	7.097386926140123E-4	0.0	0.2717642027255543	0.0
50-51	0.0	7.097386926140123E-4	0.0	0.33440521400330214	0.0
52-53	0.0	7.097386926140123E-4	0.0	0.3983079829568082	0.0
54-55	0.0	7.097386926140123E-4	0.0	0.4679938079242062	0.0
56-57	0.0	7.097386926140123E-4	0.0	0.5501789198670843	0.0
58-59	0.0	7.097386926140123E-4	0.0	0.6462433662433031	0.0
60-61	0.0	7.097386926140123E-4	0.0	0.7511664029680747	0.0
62-63	0.0	7.097386926140123E-4	0.0	0.8579820762064835	0.0
64-65	0.0	7.097386926140123E-4	0.0	0.9730780341920557	0.0
66-67	0.0	7.097386926140123E-4	0.0	1.1034596606870744	0.0
68-69	0.0	7.097386926140123E-4	0.0	1.2510195922051763	0.0
70-71	0.0	7.097386926140123E-4	0.0	1.4171510195133452	0.0
72-73	0.0	7.22882001736494E-4	0.0	1.5938890972833568	0.0
74-75	0.0	7.360253108589757E-4	0.0	1.77301925731366	0.0
76-77	0.0	7.623119291039391E-4	0.0	1.9668699235611427	0.0
78-79	0.0	7.623119291039391E-4	0.0	2.1863368992883423	0.0
80-81	0.0	7.623119291039391E-4	0.0	2.43042129300195	0.0
82-83	0.0	7.885985473489025E-4	0.0	2.682877974626579	0.0
84-85	0.0	8.411717838388294E-4	0.0	2.934401481303511	0.0
86-87	0.0	8.806017112062745E-4	0.0	3.1991208703394154	0.0
88	0.0	8.937450203287562E-4	0.0	3.4208353519265593	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3705	0.0	32.928703	1
TCAACGC	3590	0.0	32.452908	4
ATCAACG	3770	0.0	31.031305	3
CAACGCA	3805	0.0	30.865694	5
GTACATG	9720	0.0	30.14305	1
TACATGG	9770	0.0	28.678135	2
AACGCAG	4180	0.0	28.198643	6
ACATGGG	9865	0.0	27.146873	3
GGTATCA	2345	0.0	26.11341	1
TATCAAC	4705	0.0	25.37893	2
ACGCAGA	4905	0.0	23.073254	7
GAGTACA	6765	0.0	22.351173	1
CATGGGG	6920	0.0	21.52036	4
CGCAGAG	5240	0.0	21.419193	8
GTATAGA	1635	0.0	20.455198	1
AGTACAT	7030	0.0	19.860964	2
TATACCG	285	9.64812E-7	16.483362	5
GTCTAGG	1165	0.0	16.173244	1
ACACCGT	610	0.0	16.166658	6
GCAGAGT	6755	0.0	16.059172	9
>>END_MODULE