FastQCFastQC Report
Wed 25 May 2016
SRR1033161_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033161_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3804217
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46650.12262707411275435No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39500.047.7705081
GTATCAA70850.040.513761
TCAACGC80750.034.6430134
ATCAACG81900.034.156573
CAACGCA83700.033.421585
AACGCAG88200.031.756766
TATCAAC91950.030.5255722
GTACATG100250.029.805881
TACATGG100200.028.1530022
ACATGGG100750.026.9726753
ACGCAGA103900.026.777177
CGCAGAG106450.026.0019138
GAGTACA68000.022.4898741
GCAGAGT123750.022.1770279
TATACCG2458.47831E-921.1087285
GGACCGT5050.020.4733926
CATGGGG70650.020.3634094
AGAGTAC108450.019.7809410-11
AGTACAT71300.019.3864822
GAGTACT79500.018.82686412-13
Produced by FastQC (version 0.11.5)