FastQCFastQC Report
Wed 25 May 2016
SRR1033160_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033160_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3221366
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC36150.029.240114
ATCAACG37650.028.0764753
GTACATG73400.027.536081
CAACGCA38950.027.2591655
GTATCAA41400.026.8566931
TAGACCG1057.447367E-526.8456235
GGTATCA28350.026.7555371
AACGCAG40550.026.173836
TACATGG74050.026.025542
ACATGGG75650.024.1582623
ACGCAGA46900.022.2295077
TATCAAC48700.022.1993272
CGCAGAG46750.021.6981078
GAGTACA52750.019.47041
CATGGGG49300.019.2490484
CCGATCG1300.0080792318.0624379
AGTACAT53200.017.405862
GCAGAGT60050.016.5013379
TCTGTCG7800.016.2561938
GTATACG2951.3417302E-615.9705131
Produced by FastQC (version 0.11.5)