##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033160_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3221366 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.69851951004636 33.0 31.0 34.0 30.0 34.0 2 31.81308891942114 34.0 31.0 34.0 30.0 34.0 3 31.977387232621194 34.0 31.0 34.0 30.0 34.0 4 35.322383113250716 37.0 35.0 37.0 33.0 37.0 5 35.43448493589366 37.0 35.0 37.0 35.0 37.0 6 35.49787916057971 37.0 37.0 37.0 35.0 37.0 7 35.442932284006226 37.0 37.0 37.0 35.0 37.0 8 35.431726168339765 37.0 37.0 37.0 35.0 37.0 9 37.10445910213245 39.0 38.0 39.0 35.0 39.0 10-11 37.097394707710954 39.0 38.0 39.0 35.0 39.0 12-13 37.045044866059925 39.0 38.0 39.0 35.0 39.0 14-15 38.514444338209316 41.0 39.0 41.0 35.0 41.0 16-17 38.528256180763066 41.0 39.0 41.0 35.0 41.0 18-19 38.52999534979881 41.0 39.0 41.0 35.0 41.0 20-21 38.56054155286918 41.0 39.0 41.0 34.5 41.0 22-23 38.526778391527074 41.0 39.0 41.0 34.0 41.0 24-25 38.52044179394704 41.0 39.0 41.0 34.0 41.0 26-27 38.47568252101748 41.0 39.0 41.0 34.0 41.0 28-29 38.44444887665667 40.5 39.0 41.0 34.0 41.0 30-31 38.44669311093493 41.0 39.0 41.0 34.0 41.0 32-33 38.51012225869398 41.0 39.0 41.0 34.0 41.0 34-35 38.48385653787865 41.0 39.0 41.0 34.0 41.0 36-37 38.412172662156365 41.0 39.0 41.0 34.0 41.0 38-39 38.34517577325892 41.0 39.0 41.0 34.0 41.0 40-41 38.29315607105805 41.0 39.0 41.0 34.0 41.0 42-43 38.19767437167959 41.0 38.5 41.0 33.0 41.0 44-45 38.09668103531234 40.0 38.0 41.0 33.0 41.0 46-47 37.988273142511595 40.0 38.0 41.0 33.0 41.0 48-49 37.88244319335338 40.0 38.0 41.0 33.0 41.0 50-51 37.77736215009409 40.0 38.0 41.0 33.0 41.0 52-53 37.64269272724677 40.0 38.0 41.0 32.5 41.0 54-55 37.50510016558193 40.0 37.0 41.0 32.0 41.0 56-57 37.36685027407628 40.0 37.0 41.0 32.0 41.0 58-59 37.184949179944155 40.0 37.0 41.0 31.5 41.0 60-61 36.97610190832088 40.0 36.0 41.0 31.0 41.0 62-63 36.731308240044754 39.0 36.0 41.0 31.0 41.0 64-65 36.4738353232759 39.0 35.0 41.0 31.0 41.0 66-67 36.191369282472095 39.0 35.0 41.0 30.5 41.0 68-69 35.893686870725034 38.0 35.0 40.0 30.0 41.0 70-71 35.55242620677067 37.0 35.0 40.0 30.0 41.0 72-73 35.18903859418644 37.0 35.0 39.5 30.0 41.0 74-75 34.8176290741257 36.5 34.5 39.0 29.0 41.0 76-77 34.48001236121571 36.0 34.0 39.0 29.0 40.5 78-79 34.09692239255024 35.5 34.0 37.5 29.0 39.5 80-81 33.71967885673345 35.0 34.0 37.0 29.0 39.0 82-83 33.47198129613338 35.0 34.0 37.0 29.0 39.0 84-85 33.178071041912034 35.0 34.0 36.0 29.0 38.0 86-87 32.89658424407534 35.0 34.0 36.0 29.0 37.0 88-89 32.50589206566407 35.0 34.0 36.0 27.5 37.0 90-91 32.298230160745476 35.0 34.0 35.0 27.0 36.5 92-93 32.10674151897052 35.0 34.0 35.0 27.0 36.0 94-95 31.925619907827922 35.0 34.0 35.0 26.5 36.0 96-97 31.746182054445228 35.0 34.0 35.0 26.0 36.0 98-99 31.551794176756072 35.0 33.0 35.0 25.5 36.0 100 31.388704667523033 35.0 33.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2837380439710486 1101 2 0.2433329170438654 1101 3 0.23524475512915188 1101 4 0.22814079180656677 1101 5 0.19060386031834753 1101 6 0.23171127327110952 1101 7 0.24354949724234842 1101 8 0.23747323476288074 1101 9 0.2851930122423667 1101 10-11 0.25313942269256984 1101 12-13 0.2692539065151891 1101 14-15 0.31655275400420635 1101 16-17 0.24642150993493317 1101 18-19 0.29790761507631913 1101 20-21 0.24350457561212835 1101 22-23 0.24964461555486395 1101 24-25 0.2067091489583106 1101 26-27 0.22877964300811016 1101 28-29 0.26421318192277 1101 30-31 0.27804777749285137 1101 32-33 0.29396701294727023 1101 34-35 0.30568043947629775 1101 36-37 0.3236077450352539 1101 38-39 0.3470917394439397 1101 40-41 0.3385748924064558 1101 42-43 0.29214481121346836 1101 44-45 0.25187464863098086 1101 46-47 0.3063360544657172 1101 48-49 0.2565888402579972 1101 50-51 0.26256348010024766 1101 52-53 0.22614044126532917 1101 54-55 0.21107635498649557 1101 56-57 0.2700020378458774 1101 58-59 0.2997974789684221 1101 60-61 0.2700843320091977 1101 62-63 0.2584377959090105 1101 64-65 0.2844572676661272 1101 66-67 0.29271648597607935 1101 68-69 0.30069103343675607 1101 70-71 0.2826136042490077 1101 72-73 0.25429639449901487 1101 74-75 0.22359398508851314 1101 76-77 0.24295858871047926 1101 78-79 0.2090635961450502 1101 80-81 0.2728775948082145 1101 82-83 0.2297700634057378 1101 84-85 0.23714788741424542 1101 86-87 0.2719595803366701 1101 88-89 0.39323127314731465 1101 90-91 0.44100516157158154 1101 92-93 0.5091812484109681 1101 94-95 0.5369751329042352 1101 96-97 0.5026500301557881 1101 98-99 0.5217069599259858 1101 100 0.5855627924210758 1102 1 0.13638282623227838 1102 2 0.15121408457545016 1102 3 0.1774333046612 1102 4 0.0985086112155642 1102 5 0.052379267834425036 1102 6 0.058839254845430844 1102 7 0.04189690074669983 1102 8 0.13095442276533475 1102 9 0.18543418471160322 1102 10-11 0.15605867813091834 1102 12-13 0.1975526135094725 1102 14-15 0.21073405059232186 1102 16-17 0.19292164226112618 1102 18-19 0.20668985640468662 1102 20-21 0.15016604400393874 1102 22-23 0.16750350916579748 1102 24-25 0.18788049513853622 1102 26-27 0.26725760992822245 1102 28-29 0.2242302267674816 1102 30-31 0.2614591441818632 1102 32-33 0.2973757686522802 1102 34-35 0.2725282165029981 1102 36-37 0.2577116977380669 1102 38-39 0.22092250076849496 1102 40-41 0.2332638855925495 1102 42-43 0.2650351918224274 1102 44-45 0.22949484333646097 1102 46-47 0.2085891772807642 1102 48-49 0.16287391943217955 1102 50-51 0.20171753076234467 1102 52-53 0.14847143869201318 1102 54-55 0.2417028655722362 1102 56-57 0.2153689036059987 1102 58-59 0.20305019165522253 1102 60-61 0.18808393917694133 1102 62-63 0.2317284958067276 1102 64-65 0.24868079253717212 1102 66-67 0.16315867815066554 1102 68-69 0.1830525097183795 1102 70-71 0.21579980318465886 1102 72-73 0.20949778500200011 1102 74-75 0.23812240512808103 1102 76-77 0.2140265167051325 1102 78-79 0.19543293286271535 1102 80-81 0.17426331177802012 1102 82-83 0.1714248775792555 1102 84-85 0.18774830711452495 1102 86-87 0.1951578888360359 1102 88-89 0.32014288896968424 1102 90-91 0.3156652538658449 1102 92-93 0.3383182186886273 1102 94-95 0.3025628613742235 1102 96-97 0.3323749693888054 1102 98-99 0.35502828083747673 1102 100 0.3765692082863801 1103 1 0.21519291676616703 1103 2 0.18740637796562964 1103 3 0.18627678854072371 1103 4 0.23649607306357012 1103 5 0.1615790977382261 1103 6 0.20585557598116822 1103 7 0.16776422570777072 1103 8 0.15298537880173058 1103 9 0.21814691541838727 1103 10-11 0.21630211911821107 1103 12-13 0.20280039894527135 1103 14-15 0.24551016422542205 1103 16-17 0.21403678947532967 1103 18-19 0.2224093734713719 1103 20-21 0.26507820238485635 1103 22-23 0.23082322467804772 1103 24-25 0.2388707670715604 1103 26-27 0.2978405714062191 1103 28-29 0.3300537436721669 1103 30-31 0.381551735240393 1103 32-33 0.3037128186981235 1103 34-35 0.2871700894389235 1103 36-37 0.26818782444132694 1103 38-39 0.2857330892571781 1103 40-41 0.26655596047169894 1103 42-43 0.22052535054206857 1103 44-45 0.20342686376273633 1103 46-47 0.26315584155430116 1103 48-49 0.2611665143565389 1103 50-51 0.20010684028957115 1103 52-53 0.16046566020734332 1103 54-55 0.13957249806134797 1103 56-57 0.12552621498145555 1103 58-59 0.1576150610824527 1103 60-61 0.178746573422238 1103 62-63 0.24855221152515838 1103 64-65 0.19260434036844742 1103 66-67 0.13980386188769955 1103 68-69 0.0729576267791785 1103 70-71 0.129258734339885 1103 72-73 0.17679543218859806 1103 74-75 0.22099080423562611 1103 76-77 0.201832514989448 1103 78-79 0.13078889690687845 1103 80-81 0.22847537885188984 1103 82-83 0.2682243904855781 1103 84-85 0.2495366735650677 1103 86-87 0.2042315502488634 1103 88-89 0.36960392883920434 1103 90-91 0.3954274183216242 1103 92-93 0.356871590974734 1103 94-95 0.3294860622018767 1103 96-97 0.3137755754025555 1103 98-99 0.36891410928330615 1103 100 0.444172968811543 1104 1 0.09842906782174055 1104 2 0.06775654918686058 1104 3 0.1163311887795615 1104 4 0.15911731330238865 1104 5 0.09497795907945772 1104 6 0.09802790551349005 1104 7 0.11498473559836242 1104 8 0.010329956963161635 1104 9 0.09712723625325026 1104 10-11 0.08739520629821129 1104 12-13 0.1116234606156965 1104 14-15 0.03713297849674291 1104 16-17 0.107140825792996 1104 18-19 0.12070360926194468 1104 20-21 -0.009289164166034425 1104 22-23 0.00764565415802565 1104 24-25 0.062217410949301666 1104 26-27 0.10904618741244576 1104 28-29 0.19005575461773105 1104 30-31 0.18113378164016325 1104 32-33 0.18046337947181001 1104 34-35 0.14540077684402064 1104 36-37 0.18735691909878227 1104 38-39 0.17416760617561522 1104 40-41 0.19974202582716316 1104 42-43 0.12182815151090409 1104 44-45 0.15217164711757647 1104 46-47 0.08080905734889399 1104 48-49 0.06307629512319579 1104 50-51 0.07561385005022458 1104 52-53 0.09004189034757815 1104 54-55 0.07617054825855973 1104 56-57 0.10310497746328195 1104 58-59 0.04869700975402935 1104 60-61 0.0909376332970524 1104 62-63 0.04558549406130652 1104 64-65 0.06132944294725462 1104 66-67 0.06439859415898042 1104 68-69 0.003478808918039533 1104 70-71 0.06515326682323064 1104 72-73 0.14539655778114735 1104 74-75 0.039883297672083984 1104 76-77 0.024637425169004246 1104 78-79 0.030815481302873593 1104 80-81 0.0846487624525949 1104 82-83 0.07567456342511036 1104 84-85 0.05408815842508119 1104 86-87 0.008696829354150282 1104 88-89 0.11898905561021422 1104 90-91 0.16528232864437342 1104 92-93 0.1437360623725894 1104 94-95 0.1491457264464664 1104 96-97 0.1154270745101087 1104 98-99 0.17230299107228575 1104 100 0.16736080884659543 1105 1 0.14740578586775754 1105 2 0.10083128981140632 1105 3 0.15246228596826228 1105 4 0.12776604572585626 1105 5 0.07684633379910366 1105 6 0.07060888565577983 1105 7 -0.008368761234649469 1105 8 0.030212581808306993 1105 9 0.10173133971329662 1105 10-11 0.0964769347442882 1105 12-13 0.11121428090067553 1105 14-15 0.14461319854189014 1105 16-17 0.1169813598543783 1105 18-19 0.15286110372794326 1105 20-21 0.1527019083714265 1105 22-23 0.18906157643462507 1105 24-25 0.20148782257901132 1105 26-27 0.19349897591382614 1105 28-29 0.24702905624469196 1105 30-31 0.2363249043776463 1105 32-33 0.25614845055023494 1105 34-35 0.19833224651475945 1105 36-37 0.21335480099201476 1105 38-39 0.1733762514326429 1105 40-41 0.17130889517248704 1105 42-43 0.15895535637185532 1105 44-45 0.20417504266345077 1105 46-47 0.22058623084787854 1105 48-49 0.20513269030274728 1105 50-51 0.24149457595708412 1105 52-53 0.17812374351096594 1105 54-55 0.11309952306149285 1105 56-57 0.1589431038024287 1105 58-59 0.195818349249123 1105 60-61 0.18081405880533197 1105 62-63 0.2336287840097242 1105 64-65 0.13572735583717588 1105 66-67 0.20892780983850656 1105 68-69 0.13238372580622837 1105 70-71 0.15508859385911933 1105 72-73 0.14088514432190635 1105 74-75 0.1645255834114181 1105 76-77 0.10711306615851157 1105 78-79 0.1666277274979464 1105 80-81 0.14833409862050928 1105 82-83 0.2101501428387209 1105 84-85 0.17842960934125784 1105 86-87 0.2921319586165865 1105 88-89 0.33820729992461906 1105 90-91 0.3070735038779162 1105 92-93 0.2581056085999194 1105 94-95 0.2770470343604252 1105 96-97 0.32942330376868867 1105 98-99 0.3450556923237329 1105 100 0.37875206423318275 1106 1 0.07947197009812257 1106 2 0.12069334318092118 1106 3 0.06576320561246263 1106 4 0.09235974390164614 1106 5 0.05925776150903772 1106 6 0.09862938919867048 1106 7 0.04712196694264037 1106 8 0.027190570651967505 1106 9 -0.018398315043228308 1106 10-11 -0.011356247827691845 1106 12-13 -0.0067221731924931305 1106 14-15 0.034145838373042636 1106 16-17 -0.004733550442615808 1106 18-19 -0.008990245586097956 1106 20-21 0.023173791380621367 1106 22-23 0.03359647768594698 1106 24-25 0.045436639102447884 1106 26-27 0.098162778114947 1106 28-29 0.05355061511699688 1106 30-31 0.012890937846663064 1106 32-33 0.041759410900006344 1106 34-35 -0.03653621421191389 1106 36-37 -0.055782467212416975 1106 38-39 -0.03135463956689932 1106 40-41 -0.01753438840960797 1106 42-43 0.0078536678447918 1106 44-45 0.02116396170460888 1106 46-47 0.018176310751393032 1106 48-49 0.03629355265763934 1106 50-51 0.03497829373184658 1106 52-53 -0.027036063514515263 1106 54-55 -0.03879630084925623 1106 56-57 -0.08530384328033591 1106 58-59 -0.08640651129663013 1106 60-61 -0.08092949614717071 1106 62-63 -0.012794403085102601 1106 64-65 -0.014075900607871006 1106 66-67 0.018239584299536205 1106 68-69 0.01950833005769681 1106 70-71 0.0019712261522073504 1106 72-73 0.07381179892766454 1106 74-75 0.028583325006479754 1106 76-77 0.022932077164092846 1106 78-79 -0.008794781170827548 1106 80-81 -0.03179141019902687 1106 82-83 -0.06510351312388707 1106 84-85 -0.06769054745054603 1106 86-87 -0.03292193856417214 1106 88-89 0.035342457470228794 1106 90-91 7.000680268731685E-4 1106 92-93 0.02220857859840919 1106 94-95 0.09024355177335863 1106 96-97 0.08279960408528453 1106 98-99 0.08569906636263269 1106 100 0.10785880326529451 1107 1 0.16697934878517273 1107 2 0.17928543469497527 1107 3 0.18424567091888377 1107 4 0.145455126035138 1107 5 0.1065477209950032 1107 6 0.05518991276463936 1107 7 0.1272891560753493 1107 8 0.14029548549917337 1107 9 0.1379191243232185 1107 10-11 0.18670806132554674 1107 12-13 0.1399098457262795 1107 14-15 0.17277697734900244 1107 16-17 0.13022089418515037 1107 18-19 0.1726524976355961 1107 20-21 0.18151729905262215 1107 22-23 0.15880225597702236 1107 24-25 0.119208843909135 1107 26-27 0.2173990610898855 1107 28-29 0.19832577139850116 1107 30-31 0.18013598358535177 1107 32-33 0.16637104447715245 1107 34-35 0.17720207613255212 1107 36-37 0.11193829760454577 1107 38-39 0.0934815627466179 1107 40-41 0.19408690620623759 1107 42-43 0.13147573241059973 1107 44-45 0.14838077594501442 1107 46-47 0.22525398403596597 1107 48-49 0.13181281057254068 1107 50-51 0.131602979793918 1107 52-53 0.08346733709777254 1107 54-55 0.12500046902736983 1107 56-57 0.14506738258997842 1107 58-59 0.17222206117949668 1107 60-61 0.12694434205334204 1107 62-63 0.21457365132469164 1107 64-65 0.12654647560434285 1107 66-67 0.1852248635897098 1107 68-69 0.08562173268652629 1107 70-71 0.17571538278201615 1107 72-73 0.21872059652248765 1107 74-75 0.22668943144289244 1107 76-77 0.19161399617269126 1107 78-79 0.18573985741100074 1107 80-81 0.16671598682016509 1107 82-83 0.16788810908732898 1107 84-85 0.2402375538895356 1107 86-87 0.27141562949390163 1107 88-89 0.3248302574298876 1107 90-91 0.29364585988373904 1107 92-93 0.3351105566365078 1107 94-95 0.31816047050697804 1107 96-97 0.34181333485944876 1107 98-99 0.3229857260630844 1107 100 0.453380811001459 1108 1 -0.0648439160359473 1108 2 0.00975014496499682 1108 3 0.045587421800313166 1108 4 -0.05763369700088816 1108 5 -0.09084849586994892 1108 6 -0.050217521466422 1108 7 -0.011571460841693693 1108 8 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-0.028406269487774694 2214 54-55 -0.12034755698120847 2214 56-57 -0.037628393290319195 2214 58-59 -0.05359163122494692 2214 60-61 -0.021644962401218493 2214 62-63 0.02613514585190302 2214 64-65 -0.029075957080152648 2214 66-67 -0.058213391225379496 2214 68-69 -0.05110608844714193 2214 70-71 -0.008020378300827247 2214 72-73 0.022226395884942463 2214 74-75 0.08376209744950813 2214 76-77 -0.0031197568676191167 2214 78-79 -0.012820841249812531 2214 80-81 0.04004243326730972 2214 82-83 -0.011552987366883372 2214 84-85 -0.014673007157568918 2214 86-87 0.05875321776994724 2214 88-89 -0.0860469490813145 2214 90-91 -0.26096258910727244 2214 92-93 -0.1829668431554694 2214 94-95 -0.22051264982517793 2214 96-97 -0.195437406347029 2214 98-99 -0.29827014494537707 2214 100 -0.2442852989570774 2215 1 -0.24931635563710586 2215 2 -0.20279530435639614 2215 3 -0.2248821863603645 2215 4 -0.2708162639646261 2215 5 -0.2122071084292827 2215 6 -0.23512094601183975 2215 7 -0.19687039025767916 2215 8 -0.1744325186481106 2215 9 -0.2660536292472102 2215 10-11 -0.27160430360672194 2215 12-13 -0.27202989644269593 2215 14-15 -0.35381831644951944 2215 16-17 -0.2949697621168781 2215 18-19 -0.272689517061913 2215 20-21 -0.2690077586327888 2215 22-23 -0.30182745187515536 2215 24-25 -0.2070433600036523 2215 26-27 -0.2630558238337457 2215 28-29 -0.23196929638743313 2215 30-31 -0.2794411216867232 2215 32-33 -0.2757211468902838 2215 34-35 -0.3051477722703808 2215 36-37 -0.4352742880759166 2215 38-39 -0.3411253555765512 2215 40-41 -0.31405970797695915 2215 42-43 -0.3511306443032396 2215 44-45 -0.303524292519036 2215 46-47 -0.2596375580248633 2215 48-49 -0.17290384163931094 2215 50-51 -0.18441285224115944 2215 52-53 -0.3538953644888636 2215 54-55 -0.2554953711987764 2215 56-57 -0.29619226287451283 2215 58-59 -0.33983716741533954 2215 60-61 -0.38484263192977863 2215 62-63 -0.23539317844936392 2215 64-65 -0.24918987218201494 2215 66-67 -0.2929919791222986 2215 68-69 -0.3322428096580481 2215 70-71 -0.29093259077816924 2215 72-73 -0.21836304687549557 2215 74-75 -0.12072267749643117 2215 76-77 -0.23404343012114737 2215 78-79 -0.20722342602646648 2215 80-81 -0.2043324176183532 2215 82-83 -0.22316264425824528 2215 84-85 -0.2612474661955204 2215 86-87 -0.3020541902849203 2215 88-89 -0.2255621639653711 2215 90-91 -0.25003907115253554 2215 92-93 -0.2931439511235894 2215 94-95 -0.28635920142231086 2215 96-97 -0.3890258406273297 2215 98-99 -0.44457882315198205 2215 100 -0.40245987019497065 2216 1 -0.060727989799524096 2216 2 -0.06478235718459757 2216 3 -0.07606465227804193 2216 4 -0.07781164766839765 2216 5 -0.053443794204717676 2216 6 -0.02659072305112886 2216 7 -0.013804838904945882 2216 8 -0.014372096632790488 2216 9 -0.08312248259748145 2216 10-11 -0.05916798383648114 2216 12-13 -0.05136762608234591 2216 14-15 -0.07070748451160114 2216 16-17 -0.05084237130466818 2216 18-19 -0.11917857948049004 2216 20-21 -0.09666936794897651 2216 22-23 -0.08465883081704106 2216 24-25 -0.10606434782013707 2216 26-27 -0.2080683181325469 2216 28-29 -0.14078678547791412 2216 30-31 -0.13423493755275473 2216 32-33 -0.11743122060168076 2216 34-35 -0.1305557944616922 2216 36-37 -0.07840626483165636 2216 38-39 -0.10257349355939027 2216 40-41 -0.10157837958708882 2216 42-43 -0.12631287465174523 2216 44-45 -0.06835249221723672 2216 46-47 -0.029286884302401006 2216 48-49 -0.028198241996236106 2216 50-51 -0.06292851060730698 2216 52-53 -0.11434817571280576 2216 54-55 -0.08694405042662368 2216 56-57 -0.06948398010662515 2216 58-59 -0.19699862805701684 2216 60-61 -0.12110872423206587 2216 62-63 -0.10014266258779969 2216 64-65 -0.10480248901976097 2216 66-67 -0.10722268729641371 2216 68-69 -0.13638732288403332 2216 70-71 -0.19748395211011882 2216 72-73 -0.09867094142431654 2216 74-75 -0.1271902365055695 2216 76-77 -0.25286149873328867 2216 78-79 -0.23858039914257034 2216 80-81 -0.20130280533781075 2216 82-83 -0.21244864537888475 2216 84-85 -0.21241502772956977 2216 86-87 -0.21863775636704474 2216 88-89 -0.14629072005259047 2216 90-91 -0.16078566896423752 2216 92-93 -0.21477776636644563 2216 94-95 -0.16110026335847039 2216 96-97 -0.17396483506123772 2216 98-99 -0.18271674353013978 2216 100 -0.20317526717871814 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 30408.0 3 5383.0 4 3444.0 5 2698.0 6 2321.0 7 2665.0 8 3142.0 9 3392.0 10 3949.0 11 4266.0 12 4823.0 13 5333.0 14 5936.0 15 6184.0 16 5900.0 17 6226.0 18 6072.0 19 6164.0 20 6446.0 21 6651.0 22 7738.0 23 9993.0 24 15245.0 25 21762.0 26 23608.0 27 20585.0 28 21128.0 29 26603.0 30 33161.0 31 39847.0 32 51881.0 33 71358.0 34 99258.0 35 147656.0 36 270576.0 37 600003.0 38 1090441.0 39 547387.0 40 1733.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7347933025343 20.10750280234229 13.207900938952074 23.949802956171336 2 14.976129034062707 20.29024334678992 41.22207037933406 23.511557239813307 3 17.907649794721753 25.69185307687505 30.210179949672117 26.190317178731082 4 11.585769944790774 16.789809304887964 40.15482060573011 31.469600144591155 5 12.158824884921431 38.506304709292685 36.71200450686869 12.622865898917196 6 30.32592136795017 39.17613277397174 18.060398261065558 12.43754759701253 7 26.689346092424763 32.69007006241292 23.27780827089144 17.342775574270878 8 24.242117528639188 37.466208443798514 20.844721376258246 17.446952651304052 9 24.455517724665498 16.937213072914954 21.586076107360796 37.02119309505875 10-11 23.188810116190506 27.33058669048788 29.830354595131 19.65024859819062 12-13 23.841214019268588 24.66030196795027 29.70370914282104 21.794774869960104 14-15 21.105286035861212 26.8930839090274 26.5005355895366 25.501094465574788 16-17 20.84112143729174 30.243898576841726 27.13677111109179 21.77820887477474 18-19 20.704269563983534 29.379857404865422 29.038769818118702 20.877103213032335 20-21 21.95886509671878 28.722286772482175 28.36967882122876 20.94916930957028 22-23 21.440704435602054 28.510178064907134 28.548677242763397 21.500440256727423 24-25 21.431493521968452 28.565424695279994 28.456685116479747 21.546396666271804 26-27 21.195166612460405 28.483391537147334 28.610521396984684 21.71092045340758 28-29 21.140177807267253 28.56927511410829 28.64824778323081 21.642299295393645 30-31 21.402443511380937 29.10133005536386 28.21009329358619 21.28613313966901 32-33 21.079345768812 28.67450788973973 28.770174972019475 21.47597136942879 34-35 21.180276665667964 28.515735728556546 28.783164718684944 21.52082288709054 36-37 21.477229121986536 28.841006207925133 28.27278981597271 21.408974854115623 38-39 21.356083333876654 28.92512487880027 28.493028240662692 21.225763546660385 40-41 21.081055657075375 28.68877066642327 28.72042187537837 21.509751801122988 42-43 21.082023435888306 28.58472710519482 28.702901552908173 21.630347906008705 44-45 21.218177429482168 28.678293710266654 28.666946593264843 21.43658226698633 46-47 21.287868192464632 28.927998142244064 28.4881626269116 21.295971038379705 48-49 21.20643024648471 28.782145489247224 28.55673357932755 21.454690684940523 50-51 21.19842199337039 28.639671670310552 28.57362388151446 21.58828245480461 52-53 21.27036888882887 28.645149613356473 28.580513700283394 21.503967797531264 54-55 21.37271826864318 29.003709877375833 28.390860672614536 21.232711181366454 56-57 21.17677061492909 28.74208156876672 28.422317574183197 21.658830242120995 58-59 21.048416187612496 28.38258604193739 28.831615899031377 21.737381871418734 60-61 21.28468101368071 28.826393845124148 28.454286859359918 21.434638281835223 62-63 21.159367425862214 28.80989171238474 28.473783345608467 21.55695751614458 64-65 21.15296798337315 28.541300936424634 28.644768641183127 21.66096243901909 66-67 21.01004187113822 28.622219766356068 28.484480857489125 21.88325750501659 68-69 21.47494422979374 28.694104842399888 28.384178956394003 21.446771971412375 70-71 21.3078211858669 28.568718919662285 28.49031862710859 21.633141267362227 72-73 21.165457531252812 28.64010293948735 28.613359161334984 21.58108036792485 74-75 21.16316903033858 28.704997323281063 28.504317130892566 21.627516515487795 76-77 21.15652547518791 28.707906118490573 28.359780607043472 21.775787799278046 78-79 21.288455449109975 28.73521541260252 28.504560327325336 21.471768810962168 80-81 21.222924933702032 28.440955808338526 28.619737198496548 21.716382059462898 82-83 21.053589023541473 28.594300923790257 28.724155572263815 21.62795448040446 84-85 21.05145110378757 28.51101987041144 28.56697602686144 21.870552998939548 86-87 21.28687706138014 28.63201424147963 28.420080809128134 21.661027888012104 88-89 21.056956119278812 28.467847412465286 28.546890673582247 21.92830579467365 90-91 21.0407759554871 28.359468322631393 28.674334415526104 21.9254213063554 92-93 21.179425508188498 28.56035680750294 28.51546072670436 21.744756957604203 94-95 20.899922507696534 28.36988077302437 28.676492975962088 22.05370374331701 96-97 20.962659675475447 28.35292150320604 28.692503908499962 21.99191491281855 98-99 20.963697859849187 28.417870328778488 28.601778439361926 22.0166533720104 100 21.143240258075476 28.25408711624295 28.41265970067317 22.190012925008407 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 367.0 1 335.5 2 326.5 3 305.0 4 220.0 5 179.0 6 173.0 7 187.5 8 192.0 9 177.5 10 179.0 11 196.0 12 238.0 13 273.5 14 304.5 15 393.5 16 522.5 17 722.5 18 1060.5 19 1580.5 20 2387.0 21 3575.0 22 5444.0 23 8467.0 24 12488.5 25 17286.5 26 23735.5 27 32172.0 28 42331.0 29 52793.0 30 62625.0 31 72834.0 32 82918.5 33 92619.5 34 101560.5 35 108187.5 36 112784.0 37 118350.0 38 122420.5 39 122138.0 40 122016.0 41 121414.0 42 118545.0 43 116115.5 44 113322.5 45 109636.0 46 106572.5 47 103127.0 48 99259.5 49 96094.0 50 92600.5 51 87813.5 52 81300.5 53 76572.0 54 73418.5 55 69470.5 56 64120.5 57 57898.5 58 52313.5 59 46862.0 60 40965.5 61 34450.0 62 28933.5 63 23858.5 64 19170.5 65 15252.5 66 11737.0 67 8823.0 68 6782.5 69 5297.5 70 3814.0 71 2579.0 72 1874.0 73 1383.5 74 1008.0 75 714.5 76 455.5 77 302.5 78 200.5 79 111.5 80 80.5 81 58.5 82 37.0 83 25.0 84 22.0 85 15.0 86 9.5 87 12.0 88 8.5 89 4.5 90 4.5 91 7.0 92 8.5 93 6.0 94 3.0 95 3.0 96 3.0 97 1.5 98 1.0 99 1.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3308844757162024 2 0.11979390109661553 3 0.06447575345365911 4 0.03886549991525334 5 0.043211482333891896 6 0.009716374978813336 7 0.0073881701116855405 8 0.016049092217400942 9 0.01862563893702237 10-11 0.02194721121412469 12-13 0.016375040898798834 14-15 0.01912232264200963 16-17 0.019929433662613936 18-19 0.019836305467928823 20-21 0.02458584339686953 22-23 0.016080134948962644 24-25 0.025687860367310016 26-27 0.01997599775995649 28-29 0.019603484981216043 30-31 0.020891758341026755 32-33 0.025532646709501495 34-35 0.01586283582803072 36-37 0.009995759562868672 38-39 0.013751930081834849 40-41 0.009933674099745264 42-43 0.008707486203057956 44-45 0.00880061439774307 46-47 0.008552272545249437 48-49 0.004718495197379 50-51 0.006627623188423793 52-53 0.007652033329960024 54-55 0.003119794521951247 56-57 0.0026075894511831317 58-59 0.0027317603774299473 60-61 0.003569914129595954 62-63 0.0025920680854022798 64-65 0.00220403394088098 66-67 0.0016763075043320131 68-69 0.003476785934910842 70-71 0.0023902903302512038 72-73 0.001738392967455421 74-75 0.0022195553066618324 76-77 0.00353887139803425 78-79 0.0032905295455406183 80-81 0.003523350032253398 82-83 0.0022350766724426843 84-85 0.002918016766800171 86-87 0.0022195553066618324 88-89 0.5998076592352437 90-91 0.6111537776210465 92-93 0.6061869405711738 94-95 0.5933042069730667 96-97 0.5913795576162411 98-99 0.5883063271916323 100 0.5912709080557751 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3221366.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.99439257868404 #Duplication Level Percentage of deduplicated Percentage of total 1 76.11784447278127 50.23350910379942 2 14.704042949055712 19.407687657476274 3 4.592685095447309 9.092743895376621 4 1.8715839163447465 4.940561748768246 5 0.9266182988547657 3.0575805892606893 6 0.5100437984074526 2.0196018398654765 7 0.3106769694805392 1.435205652233818 8 0.21805190795935483 1.1512162573120595 9 0.1431283235236984 0.8501100094576667 >10 0.579228263101214 6.326893419411271 >50 0.0203529720406181 0.9039854825396787 >100 0.005648829740607101 0.5487941226212164 >500 9.420326270682333E-5 0.032110221877526386 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.104273156170395E-5 0.0 20-21 0.0 0.0 0.0 9.312819468511184E-5 0.0 22-23 0.0 0.0 0.0 1.241709262468158E-4 0.0 24-25 0.0 0.0 0.0 2.0177775515107567E-4 0.0 26-27 0.0 0.0 0.0 4.1907687608300334E-4 0.0 28-29 0.0 0.0 0.0 0.0011485810677830462 0.0 30-31 0.0 0.0 0.0 0.003600956861157658 0.0 32-33 0.0 0.0 0.0 0.010585571462541046 0.0 34-35 0.0 0.0 0.0 0.020845194243684202 0.0 36-37 0.0 0.0 0.0 0.039113841767746974 0.0 38-39 0.0 0.0 0.0 0.07085503478958927 0.0 40-41 0.0 0.0 0.0 0.10810631266363399 0.0 42-43 0.0 1.5521365780851974E-5 0.0 0.14796517998886188 0.0 44-45 0.0 3.104273156170395E-5 0.0 0.19244941431678364 0.0 46-47 0.0 3.104273156170395E-5 0.0 0.2502044163873338 0.0 48-49 0.0 3.104273156170395E-5 0.0 0.3146801698409929 0.0 50-51 0.0 3.104273156170395E-5 0.0 0.38322252112923527 0.0 52-53 0.0 3.104273156170395E-5 0.0 0.4519666501726286 0.0 54-55 0.0 3.104273156170395E-5 0.0 0.527524658793816 0.0 56-57 0.0 3.104273156170395E-5 0.0 0.6168501188626192 1.5521365780851974E-5 58-59 0.0 3.104273156170395E-5 0.0 0.7155970479603995 3.104273156170395E-5 60-61 0.0 3.104273156170395E-5 0.0 0.8276613088981506 3.104273156170395E-5 62-63 0.0 3.104273156170395E-5 0.0 0.938980544278421 3.104273156170395E-5 64-65 0.0 3.104273156170395E-5 0.0 1.0556546508530853 3.104273156170395E-5 66-67 0.0 3.104273156170395E-5 0.0 1.18654632848301 3.104273156170395E-5 68-69 0.0 3.104273156170395E-5 0.0 1.3393696959612784 3.104273156170395E-5 70-71 0.0 3.104273156170395E-5 0.0 1.5047498483562562 3.104273156170395E-5 72-73 0.0 3.104273156170395E-5 0.0 1.6760591624795196 3.104273156170395E-5 74-75 0.0 3.104273156170395E-5 0.0 1.8489826986439915 3.104273156170395E-5 76-77 0.0 3.104273156170395E-5 0.0 2.036496318642464 3.104273156170395E-5 78-79 0.0 3.104273156170395E-5 0.0 2.257101490485713 3.104273156170395E-5 80-81 0.0 3.104273156170395E-5 0.0 2.497760266917823 3.104273156170395E-5 82-83 0.0 3.104273156170395E-5 0.0 2.7515035547031914 3.104273156170395E-5 84-85 0.0 3.104273156170395E-5 0.0 2.996648005845967 3.104273156170395E-5 86-87 0.0 3.104273156170395E-5 0.0 3.2595333780762568 3.104273156170395E-5 88 0.0 3.104273156170395E-5 0.0 3.4830255239547445 3.104273156170395E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAACGC 3615 0.0 29.24011 4 ATCAACG 3765 0.0 28.076475 3 GTACATG 7340 0.0 27.53608 1 CAACGCA 3895 0.0 27.259165 5 GTATCAA 4140 0.0 26.856693 1 TAGACCG 105 7.447367E-5 26.845623 5 GGTATCA 2835 0.0 26.755537 1 AACGCAG 4055 0.0 26.17383 6 TACATGG 7405 0.0 26.02554 2 ACATGGG 7565 0.0 24.158262 3 ACGCAGA 4690 0.0 22.229507 7 TATCAAC 4870 0.0 22.199327 2 CGCAGAG 4675 0.0 21.698107 8 GAGTACA 5275 0.0 19.4704 1 CATGGGG 4930 0.0 19.249048 4 CCGATCG 130 0.00807923 18.062437 9 AGTACAT 5320 0.0 17.40586 2 GCAGAGT 6005 0.0 16.501337 9 TCTGTCG 780 0.0 16.256193 8 GTATACG 295 1.3417302E-6 15.970513 1 >>END_MODULE