FastQCFastQC Report
Wed 25 May 2016
SRR1033160_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033160_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3221366
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52450.1628191270411372No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44650.13860579642300813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48200.044.3177571
GTATCAA81900.039.1230051
TCAACGC92250.033.631684
ATCAACG92500.033.540783
CAACGCA93750.033.093575
AACGCAG98350.031.7280436
TATCAAC99850.031.4955352
ACGCAGA109550.028.312257
CGCAGAG111600.027.792188
GTACATG75950.025.771191
TACATGG76100.024.3378682
GCAGAGT128550.023.7986189
ACATGGG77900.022.9306893
GAGTACT85250.021.19555712-13
TATACCG1605.4097327E-520.5659625
AGAGTAC112300.020.29573610-11
GAGTACA57100.020.2706621
CAGAGTA125600.019.73675210-11
CATGGGG52400.019.5567264
AGTATCG1751.0770933E-418.8031645
Produced by FastQC (version 0.11.5)