##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033160_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3221366 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.73970917927364 34.0 31.0 34.0 31.0 34.0 2 32.822276947108776 34.0 31.0 34.0 31.0 34.0 3 32.92712097911259 34.0 31.0 34.0 31.0 34.0 4 36.360407355140644 37.0 37.0 37.0 35.0 37.0 5 36.301643774721654 37.0 37.0 37.0 35.0 37.0 6 36.38365774022573 37.0 37.0 37.0 35.0 37.0 7 36.35277115360378 37.0 37.0 37.0 35.0 37.0 8 36.344515649572266 37.0 37.0 37.0 35.0 37.0 9 38.16445756241296 39.0 39.0 39.0 37.0 39.0 10-11 38.1428375415895 39.0 39.0 39.0 37.0 39.0 12-13 38.10567815020087 39.0 39.0 39.0 37.0 39.0 14-15 39.624972449575736 41.0 40.0 41.0 37.0 41.0 16-17 39.638902564936735 41.0 40.0 41.0 37.0 41.0 18-19 39.65807020996682 41.0 40.0 41.0 37.0 41.0 20-21 39.660258722541926 41.0 40.0 41.0 37.0 41.0 22-23 39.59308318272434 41.0 40.0 41.0 37.0 41.0 24-25 39.54995489491104 41.0 40.0 41.0 37.0 41.0 26-27 39.45091042123124 41.0 39.0 41.0 36.5 41.0 28-29 39.36335253429756 41.0 39.0 41.0 36.0 41.0 30-31 39.28127881153523 41.0 39.0 41.0 36.0 41.0 32-33 39.32218475019603 41.0 39.0 41.0 36.0 41.0 34-35 39.27241797423825 41.0 39.0 41.0 35.5 41.0 36-37 39.19221690425739 41.0 39.0 41.0 35.0 41.0 38-39 39.110116019104936 41.0 39.0 41.0 35.0 41.0 40-41 39.00551163698878 41.0 39.0 41.0 35.0 41.0 42-43 38.90247615452575 41.0 39.0 41.0 35.0 41.0 44-45 38.774885095329125 41.0 39.0 41.0 35.0 41.0 46-47 38.584565367611134 41.0 39.0 41.0 35.0 41.0 48-49 38.41639261108486 41.0 39.0 41.0 35.0 41.0 50-51 38.303663880478034 41.0 38.0 41.0 34.5 41.0 52-53 38.181021187906 40.0 38.0 41.0 34.0 41.0 54-55 38.05866082897752 40.0 38.0 41.0 34.0 41.0 56-57 37.914846062198464 40.0 38.0 41.0 34.0 41.0 58-59 37.74285473926278 40.0 37.0 41.0 34.0 41.0 60-61 37.56540222377712 40.0 37.0 41.0 33.0 41.0 62-63 37.349532775847265 40.0 36.0 41.0 33.0 41.0 64-65 37.1104818576964 39.0 36.0 41.0 33.0 41.0 66-67 36.844343983266725 39.0 35.0 41.0 33.0 41.0 68-69 36.52718815558369 39.0 35.0 41.0 32.0 41.0 70-71 36.19937116738676 37.5 35.0 40.0 32.0 41.0 72-73 35.85011855219183 37.0 35.0 40.0 31.5 41.0 74-75 35.50912873607035 37.0 35.0 39.0 31.0 41.0 76-77 35.16287391746235 36.0 35.0 39.0 31.0 41.0 78-79 34.80774801745595 36.0 35.0 38.5 31.0 40.0 80-81 34.451155193169605 35.0 35.0 37.0 31.0 39.0 82-83 34.109701598638594 35.0 34.5 37.0 31.0 39.0 84-85 33.81958864655553 35.0 34.0 36.5 30.0 38.5 86-87 33.56799739613568 35.0 34.0 36.0 30.0 37.0 88-89 33.33346241935874 35.0 34.0 36.0 30.0 37.0 90-91 33.12097725002375 35.0 34.0 35.5 30.0 36.5 92-93 32.94502223590862 35.0 34.0 35.0 30.0 36.0 94-95 32.78794322036056 35.0 34.0 35.0 29.5 36.0 96-97 32.657054802217445 35.0 34.0 35.0 29.5 36.0 98-99 32.5424017326811 35.0 34.0 35.0 29.0 36.0 100 32.439566010195676 35.0 34.0 35.0 29.0 36.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.11640238772103118 1101 2 0.13650017946052628 1101 3 0.14027157112558797 1101 4 0.06352772890625857 1101 5 0.07975192216916582 1101 6 0.05809306028236705 1101 7 0.11052565078713883 1101 8 0.1265813028448477 1101 9 0.16077342008366458 1101 10-11 0.13456834514675364 1101 12-13 0.19962808032931179 1101 14-15 0.2525606667861098 1101 16-17 0.22126455916009036 1101 18-19 0.23272059355735308 1101 20-21 0.23460338845661255 1101 22-23 0.22057440395392547 1101 24-25 0.17380321436991863 1101 26-27 0.17159470073230665 1101 28-29 0.18686363244462 1101 30-31 0.21513995712071932 1101 32-33 0.2112713012625207 1101 34-35 0.22598734132579779 1101 36-37 0.22510516891384924 1101 38-39 0.24848630341145395 1101 40-41 0.2994567084686466 1101 42-43 0.2133257251368761 1101 44-45 0.23124368544360863 1101 46-47 0.3021171803777918 1101 48-49 0.21859754190839453 1101 50-51 0.20275267505876116 1101 52-53 0.22136064382164022 1101 54-55 0.2669094264307361 1101 56-57 0.2505053562142834 1101 58-59 0.23130802372818948 1101 60-61 0.21653194345959292 1101 62-63 0.2131518307698812 1101 64-65 0.24357916079245 1101 66-67 0.23588689240075666 1101 68-69 0.26820764553924903 1101 70-71 0.2373953858352209 1101 72-73 0.28187649860639397 1101 74-75 0.25955213340778016 1101 76-77 0.25287238240941434 1101 78-79 0.23985017868673708 1101 80-81 0.24524999175970663 1101 82-83 0.23352090212679855 1101 84-85 0.2818567644107901 1101 86-87 0.2646756794935001 1101 88-89 0.2770830558603379 1101 90-91 0.30867037858253354 1101 92-93 0.29031525595448215 1101 94-95 0.3300971076577568 1101 96-97 0.30906344573404 1101 98-99 0.29406877217993355 1101 100 0.30130966856811625 1102 1 0.07371981253263016 1102 2 0.08557713027665415 1102 3 0.10013491857292678 1102 4 0.047524766316065836 1102 5 0.022219719045985187 1102 6 0.05592935537662669 1102 7 0.061194543661216017 1102 8 0.09907792716476393 1102 9 0.169491547775948 1102 10-11 0.10993138920343881 1102 12-13 0.14055786073418375 1102 14-15 0.17408038741555742 1102 16-17 0.13971215621370447 1102 18-19 0.16551379438443092 1102 20-21 0.14645239871124005 1102 22-23 0.16035456952632643 1102 24-25 0.14426099663044312 1102 26-27 0.1834804827397889 1102 28-29 0.20661593962332603 1102 30-31 0.18803718504706524 1102 32-33 0.18461493163437837 1102 34-35 0.17594423165807882 1102 36-37 0.22852458653172647 1102 38-39 0.21573219626491635 1102 40-41 0.26990830681969413 1102 42-43 0.2514201948974417 1102 44-45 0.22664411253614247 1102 46-47 0.24316715608048156 1102 48-49 0.22992942569543828 1102 50-51 0.19399251573661047 1102 52-53 0.2086452702199253 1102 54-55 0.18846568188164525 1102 56-57 0.1456824754649304 1102 58-59 0.1817077862281593 1102 60-61 0.22456451015400347 1102 62-63 0.1981898025802593 1102 64-65 0.1826938571494594 1102 66-67 0.16378338465511888 1102 68-69 0.2406970197584073 1102 70-71 0.24176042035256984 1102 72-73 0.2345399036754401 1102 74-75 0.216656935983508 1102 76-77 0.19797405085662234 1102 78-79 0.19960444291879753 1102 80-81 0.18050521781476903 1102 82-83 0.16562558031354513 1102 84-85 0.14390990291069272 1102 86-87 0.1636257653929789 1102 88-89 0.24005664914532332 1102 90-91 0.1904837377698243 1102 92-93 0.17275491959719602 1102 94-95 0.1743821683512934 1102 96-97 0.152528036692118 1102 98-99 0.18825006876804906 1102 100 0.1600751470001356 1103 1 0.11989715807460044 1103 2 0.14415640357559312 1103 3 0.08717152607409417 1103 4 0.06781357089320039 1103 5 0.03343663404574926 1103 6 0.09735031218618673 1103 7 0.07860786639115247 1103 8 0.0926660918561808 1103 9 0.13039266186926568 1103 10-11 0.1466191982391578 1103 12-13 0.08946171587997043 1103 14-15 0.15473546489769774 1103 16-17 0.1675041086751321 1103 18-19 0.11127540289808024 1103 20-21 0.11946177609285513 1103 22-23 0.1387007153009776 1103 24-25 0.137474590704322 1103 26-27 0.16481488653786158 1103 28-29 0.15380702816539582 1103 30-31 0.10410690424106406 1103 32-33 0.1419087958372245 1103 34-35 0.15145942158043368 1103 36-37 0.12555446105816515 1103 38-39 0.09293329912894421 1103 40-41 0.15609984085182305 1103 42-43 0.1165897986690112 1103 44-45 0.12906904193885538 1103 46-47 0.12647931943174484 1103 48-49 0.05835484384860479 1103 50-51 0.03082577635565542 1103 52-53 0.03982338432428634 1103 54-55 0.026990401057226165 1103 56-57 0.030020784664486655 1103 58-59 0.06506205943505705 1103 60-61 -0.026494679274989608 1103 62-63 -0.07232305864406641 1103 64-65 -0.04739326653110254 1103 66-67 -0.03609051588646395 1103 68-69 0.021544951175037852 1103 70-71 0.07082404443259804 1103 72-73 9.563733692345977E-4 1103 74-75 -0.008029334086558038 1103 76-77 -0.04023263536006283 1103 78-79 -0.09853002144384249 1103 80-81 -0.09252581277677763 1103 82-83 -0.07277566264053803 1103 84-85 -0.06430416518669091 1103 86-87 -0.06564734946613981 1103 88-89 -0.1218788716914645 1103 90-91 -0.0937252964740054 1103 92-93 -0.08953740748086858 1103 94-95 -0.06780316287608912 1103 96-97 -0.047048471623085675 1103 98-99 0.03893915168442419 1103 100 0.05049812324189418 1104 1 0.06965206793197609 1104 2 0.1051922973876458 1104 3 0.12983614796621623 1104 4 0.05476018946184524 1104 5 0.033888805938545374 1104 6 0.04893928135776804 1104 7 0.024755514005470047 1104 8 0.08650823110847483 1104 9 0.07308576991941607 1104 10-11 0.051322886708874194 1104 12-13 0.08125031010462891 1104 14-15 0.11312694740220053 1104 16-17 0.14031419164744818 1104 18-19 0.10357624232242557 1104 20-21 0.12056202374255065 1104 22-23 0.13740492176592767 1104 24-25 0.11800130808941134 1104 26-27 0.09454923141171179 1104 28-29 0.12734651518902496 1104 30-31 0.1356095253613745 1104 32-33 0.11675159394938817 1104 34-35 0.18229452422367132 1104 36-37 0.10804193985688215 1104 38-39 0.1296651617782274 1104 40-41 0.18207766424801974 1104 42-43 0.1755597927122423 1104 44-45 0.18032126051208053 1104 46-47 0.18486815736832796 1104 48-49 0.11543909539739872 1104 50-51 0.177144416988682 1104 52-53 0.20368513096698138 1104 54-55 0.14790385003201578 1104 56-57 0.1890493932512527 1104 58-59 0.19127932138457027 1104 60-61 0.1357208350223189 1104 62-63 0.13522869523696102 1104 64-65 0.1745181779417493 1104 66-67 0.15659494036817279 1104 68-69 0.15839650848993614 1104 70-71 0.169670207365904 1104 72-73 0.20647064651846847 1104 74-75 0.2093228708118815 1104 76-77 0.1730934497987846 1104 78-79 0.18137820712701114 1104 80-81 0.23297890591994985 1104 82-83 0.18168922716287028 1104 84-85 0.1872732904937493 1104 86-87 0.16788115387488034 1104 88-89 0.2116123078914356 1104 90-91 0.18764742311194027 1104 92-93 0.18366250091236935 1104 94-95 0.08755030174952338 1104 96-97 0.10514395125576925 1104 98-99 0.13684324882267873 1104 100 0.11225813644414728 1105 1 0.054193254384856004 1105 2 0.04152111585040075 1105 3 0.057987242712748355 1105 4 0.044405653144060864 1105 5 0.04935427281436233 1105 6 0.03479795735057678 1105 7 -0.029344267139151725 1105 8 -0.03748802945987961 1105 9 -0.011114322548806399 1105 10-11 -0.049884418489490656 1105 12-13 -0.006473567883240605 1105 14-15 0.004923340932762699 1105 16-17 -2.1885217476835805E-4 1105 18-19 -0.019056477234819624 1105 20-21 0.03622501620628782 1105 22-23 0.08511164982063946 1105 24-25 0.047795709795998675 1105 26-27 0.03556512425711844 1105 28-29 0.07170916282769468 1105 30-31 0.1391545754413741 1105 32-33 0.06162530855237236 1105 34-35 0.11440121856818308 1105 36-37 0.0658343967041688 1105 38-39 0.0580135081520865 1105 40-41 0.15778244744343795 1105 42-43 0.15546308723188673 1105 44-45 0.15568157087550816 1105 46-47 0.15023497233899263 1105 48-49 0.11379207040071293 1105 50-51 0.07402258384267668 1105 52-53 0.11284051567548659 1105 54-55 0.11427192574323897 1105 56-57 0.10240452862952765 1105 58-59 0.06687962122791191 1105 60-61 0.018480247003743955 1105 62-63 0.018463233161973847 1105 64-65 -0.007563794720638839 1105 66-67 0.02669495946713596 1105 68-69 0.024735766155878025 1105 70-71 0.01927575629422762 1105 72-73 0.06016420933163147 1105 74-75 0.04539170067140219 1105 76-77 -0.02688728163946763 1105 78-79 0.017898289221264463 1105 80-81 0.040195641102855006 1105 82-83 -0.00843192342988175 1105 84-85 -0.0236380772532101 1105 86-87 0.019870029509291953 1105 88-89 -0.0061433782259143754 1105 90-91 0.030416154971838694 1105 92-93 0.008756143252377058 1105 94-95 0.019392754578731797 1105 96-97 0.061784142892513216 1105 98-99 0.07579837094647957 1105 100 0.07860064249997833 1106 1 0.019445025830655993 1106 2 0.007891536474552652 1106 3 0.005885629566357409 1106 4 0.02447877239628582 1106 5 -0.04313479698814149 1106 6 -0.02297228235995874 1106 7 -0.051114959133720106 1106 8 0.010190999637060827 1106 9 0.005908140891570213 1106 10-11 -0.04567516174292052 1106 12-13 -0.018425173491564806 1106 14-15 0.03363810046842275 1106 16-17 -0.028142114650272276 1106 18-19 0.01680437004394264 1106 20-21 -0.023694703377607595 1106 22-23 0.021609840684511994 1106 24-25 0.011685729394976363 1106 26-27 -0.05471604565091326 1106 28-29 -0.08795991602048758 1106 30-31 0.018269606347445233 1106 32-33 0.02165131488435179 1106 34-35 -0.01694583404752592 1106 36-37 0.03468031860979437 1106 38-39 0.018649721027102828 1106 40-41 0.0726708840482928 1106 42-43 0.08647168062854149 1106 44-45 0.12471707517220665 1106 46-47 0.1982833667306707 1106 48-49 0.10516626005848906 1106 50-51 0.06731408090283253 1106 52-53 0.06572872613832459 1106 54-55 0.028832456423174335 1106 56-57 0.004243817035984421 1106 58-59 0.005097999035839962 1106 60-61 0.027308454451350883 1106 62-63 0.04569789319513973 1106 64-65 0.06441004818603346 1106 66-67 0.030041107514783505 1106 68-69 0.03019294993395505 1106 70-71 -0.01132578147149843 1106 72-73 0.06151352336750904 1106 74-75 0.0742719207829623 1106 76-77 0.03570769800774798 1106 78-79 0.06436858923633793 1106 80-81 0.04749060567394281 1106 82-83 0.08240894797069132 1106 84-85 0.06355587721686362 1106 86-87 0.05669725700816031 1106 88-89 0.03494926462045811 1106 90-91 0.03527632382454726 1106 92-93 0.036919870072821936 1106 94-95 -0.015573701022056241 1106 96-97 0.016720446224340435 1106 98-99 0.03337902826594075 1106 100 0.10666335921941084 1107 1 -0.018849351701469175 1107 2 -0.0059250321151580465 1107 3 0.02555638430063567 1107 4 -0.016761213671287578 1107 5 -0.08999285121461753 1107 6 -0.08615730866658566 1107 7 -0.10309868019185586 1107 8 -0.03786011037411896 1107 9 -0.010150448633446274 1107 10-11 -1.9788443260893018E-4 1107 12-13 -0.009626662061471336 1107 14-15 0.004585872196585683 1107 16-17 0.012156882581578543 1107 18-19 0.017070736063487857 1107 20-21 -0.01610464673129286 1107 22-23 -0.024191915534672148 1107 24-25 0.02212570079684184 1107 26-27 0.06673677658230304 1107 28-29 0.06527167392616917 1107 30-31 0.04442480625945677 1107 32-33 0.03249257686898943 1107 34-35 0.07360211366921021 1107 36-37 0.12296443014254521 1107 38-39 0.1033817967755084 1107 40-41 0.06422911609654847 1107 42-43 0.023039903694041186 1107 44-45 0.06891128588184614 1107 46-47 0.022611218753624485 1107 48-49 0.04138452253808822 1107 50-51 0.08035153346841639 1107 52-53 0.1064084819541975 1107 54-55 0.13803995793557533 1107 56-57 0.11205605924023843 1107 58-59 0.11373115454740201 1107 60-61 0.10608423402567979 1107 62-63 0.107488576800705 1107 64-65 0.08254016972814071 1107 66-67 0.11889314879876167 1107 68-69 0.14996544805490686 1107 70-71 0.14441382878717235 1107 72-73 0.17046419157407655 1107 74-75 0.11667416463711078 1107 76-77 0.1059401495650647 1107 78-79 0.10272126297920892 1107 80-81 0.10536792698138697 1107 82-83 0.14063952964955462 1107 84-85 0.13696675550977488 1107 86-87 0.11615847536230461 1107 88-89 0.14507703964842023 1107 90-91 0.09507302037260246 1107 92-93 0.11820586981617254 1107 94-95 0.0999712218912876 1107 96-97 0.09766436820274293 1107 98-99 0.15573764162274273 1107 100 0.136124577573554 1108 1 0.02609070338542807 1108 2 0.02693128460521166 1108 3 -0.0104412758514556 1108 4 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-0.04139336751872946 2214 8 0.012266845686070837 2214 9 -0.01972591114576261 2214 10-11 -0.016363521298217165 2214 12-13 -0.043980390213384624 2214 14-15 -0.015480216506773559 2214 16-17 -0.0592624495685925 2214 18-19 -0.08207958693170525 2214 20-21 -0.07725494236834152 2214 22-23 -0.1478409851286031 2214 24-25 -0.15150066304224907 2214 26-27 -0.16098450738341086 2214 28-29 -0.09509974517299469 2214 30-31 -0.008706566111392533 2214 32-33 -0.024132501663537198 2214 34-35 -0.05883829574990074 2214 36-37 -0.08284611983352619 2214 38-39 -0.14622526814903836 2214 40-41 -0.11329206260045765 2214 42-43 -0.11520355100196156 2214 44-45 -0.15480456689136446 2214 46-47 -0.09492704525349893 2214 48-49 -0.15202205996620677 2214 50-51 -0.01901062722954805 2214 52-53 -0.020253835218817073 2214 54-55 -0.05278306517371334 2214 56-57 -0.03924775165126704 2214 58-59 -0.14627450347717286 2214 60-61 -0.13348746599049122 2214 62-63 -0.16031693266314306 2214 64-65 -0.06923128501176734 2214 66-67 -0.16859049362520295 2214 68-69 -0.03248654723678612 2214 70-71 -0.019523600135208596 2214 72-73 -0.09067666024272825 2214 74-75 -0.09030432998412152 2214 76-77 -0.03769449387002766 2214 78-79 -0.242648469159775 2214 80-81 -0.15822495470058584 2214 82-83 -0.13556990880428543 2214 84-85 -0.14026797441913885 2214 86-87 -0.0521948696253034 2214 88-89 -0.2433889818020205 2214 90-91 -0.09300593973539151 2214 92-93 -0.08822061415823157 2214 94-95 -0.12443390198810533 2214 96-97 -0.290009619998834 2214 98-99 -0.4043675391600843 2214 100 -0.5484937278646207 2215 1 -0.06066112416487357 2215 2 -0.1012275302993828 2215 3 -0.07600540466980732 2215 4 -0.060479214552849214 2215 5 -0.05323290193138064 2215 6 -0.08816759835173826 2215 7 -0.02176325626432174 2215 8 -0.030744461165653547 2215 9 -0.07191008562134726 2215 10-11 -0.05738562924984336 2215 12-13 -0.07838539517744891 2215 14-15 -0.16468171270759058 2215 16-17 -0.16705245701194116 2215 18-19 -0.12689624227392216 2215 20-21 -0.15149237031244667 2215 22-23 -0.17078713795140743 2215 24-25 -0.14094165059965746 2215 26-27 -0.12351777980663314 2215 28-29 -0.14146767055700593 2215 30-31 -0.17601047320917473 2215 32-33 -0.2028221811378188 2215 34-35 -0.09285481321977329 2215 36-37 -0.10153258476018578 2215 38-39 -0.15081651210063285 2215 40-41 -0.19824301154481105 2215 42-43 -0.16123559120483577 2215 44-45 -0.11597636364069785 2215 46-47 -0.18510482938982875 2215 48-49 -0.12934053354123165 2215 50-51 -0.01376809036739246 2215 52-53 -0.07881505235456387 2215 54-55 -0.04030594082363592 2215 56-57 -0.14366279331531473 2215 58-59 -0.15650061989684616 2215 60-61 -0.08170971395841065 2215 62-63 -0.12328194947939153 2215 64-65 -0.11799843934413445 2215 66-67 -0.12614564943525153 2215 68-69 -0.10252661295154297 2215 70-71 -0.003925897677810042 2215 72-73 0.028219856611457317 2215 74-75 0.018906317850984067 2215 76-77 -0.02429286067643943 2215 78-79 -0.005280892304739382 2215 80-81 -0.011927133666361556 2215 82-83 0.09395896137911564 2215 84-85 -0.027610655365599257 2215 86-87 -0.05873583497360357 2215 88-89 0.00732724799402007 2215 90-91 -0.04470602175514671 2215 92-93 -0.0026039075261721223 2215 94-95 -0.025591578916035473 2215 96-97 0.06286373754461749 2215 98-99 0.039626905266032963 2215 100 0.0459770715641028 2216 1 -0.058660229969007105 2216 2 -0.07384530011204049 2216 3 -0.10868418072008268 2216 4 -0.08131896090099389 2216 5 -0.02681876837198871 2216 6 -0.07404553537821101 2216 7 -0.06669428177186632 2216 8 -0.05691741836190545 2216 9 -0.15640370457088437 2216 10-11 -0.10227680005280604 2216 12-13 -0.06162083434339394 2216 14-15 -0.1625692286964764 2216 16-17 -0.13250651994893303 2216 18-19 -0.1561025722620144 2216 20-21 -0.16598313127339992 2216 22-23 -0.158408407725652 2216 24-25 -0.21755699837442677 2216 26-27 -0.24718373842274133 2216 28-29 -0.2571939978697557 2216 30-31 -0.24290186064445152 2216 32-33 -0.17861288236036188 2216 34-35 -0.1420848586037735 2216 36-37 -0.1986450862308189 2216 38-39 -0.2059840690683501 2216 40-41 -0.2335319014096413 2216 42-43 -0.23232223190786527 2216 44-45 -0.17065174690394969 2216 46-47 -0.13749996283156918 2216 48-49 -0.11768036315783803 2216 50-51 -0.0664881924222982 2216 52-53 0.010657231737020822 2216 54-55 -0.016242110039122792 2216 56-57 -0.004310953028529241 2216 58-59 -0.013395017084192773 2216 60-61 -0.007867122111953506 2216 62-63 0.0027735118843139617 2216 64-65 0.05216444518829633 2216 66-67 0.02970610570281451 2216 68-69 0.05169143505837326 2216 70-71 0.009085102086352492 2216 72-73 0.04881441426519473 2216 74-75 0.02615369610309415 2216 76-77 0.030931305416750376 2216 78-79 0.05008828850547786 2216 80-81 0.07568577904303453 2216 82-83 0.06169041682055365 2216 84-85 0.09511457105747922 2216 86-87 0.06342920956965514 2216 88-89 0.08349106580477184 2216 90-91 0.04151306751347761 2216 92-93 0.09280170793790177 2216 94-95 0.005908209689508226 2216 96-97 0.024164785814008383 2216 98-99 0.0774514038152816 2216 100 0.11364683447460777 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 13.0 3 9.0 4 14.0 5 22.0 6 52.0 7 161.0 8 284.0 9 689.0 10 1209.0 11 1989.0 12 3023.0 13 4818.0 14 5736.0 15 7378.0 16 9170.0 17 13164.0 18 15452.0 19 8474.0 20 5784.0 21 5361.0 22 5643.0 23 6107.0 24 6931.0 25 8589.0 26 10596.0 27 13831.0 28 18006.0 29 23080.0 30 29814.0 31 38332.0 32 49232.0 33 64285.0 34 89681.0 35 136170.0 36 247819.0 37 584071.0 38 1165273.0 39 638217.0 40 2887.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.67840813438742 20.145652863575194 13.41652536698301 23.759413635054376 2 15.320991440807715 20.45796583525076 40.73383308519581 23.487209638745714 3 17.914015358701867 25.72889885843459 30.583392262785413 25.77369352007813 4 11.752392855069644 16.972732953555965 39.889382158659025 31.38549203271537 5 11.940977538173925 38.276218661979485 36.73774040178502 13.045063398061568 6 30.169400501651477 39.31845555914272 17.751725979089578 12.760417960116225 7 26.37601983590735 32.97744432326916 23.206235880777182 17.440299960046303 8 24.577592610815877 36.91105953174392 20.59281228376737 17.918535573672834 9 24.740947922616535 16.19157747038518 21.714400129138003 37.35307447786028 10-11 23.356499561054413 26.940068095547343 29.685573404491773 20.017858938906475 12-13 24.342095868647025 24.32374961429406 29.85643357507343 21.47772094198548 14-15 21.30906348987572 26.34011555353571 27.173194336312196 25.177626620276378 16-17 20.4840741474269 29.900359040233244 27.744348205078218 21.87121860726164 18-19 20.258493106565933 28.51456487743928 30.477026413512913 20.74991560248187 20-21 21.417668777779365 27.86476296080607 30.08891259173903 20.628655669675535 22-23 20.96015422069733 27.639543267085383 30.270592444334415 21.129710067882872 24-25 20.913637683658585 27.779392862869134 30.190389817730967 21.116579635741314 26-27 20.726057075413564 27.6938904181116 30.30496532939788 21.275087177076955 28-29 20.695455832424738 27.778184816596585 30.33450415634927 21.191855194629404 30-31 20.958417889266535 28.27094438173374 29.89474436030687 20.875893368692854 32-33 20.663617315660304 27.90716629571853 30.36720760509921 21.062008783521954 34-35 20.74243062205711 27.776188985727572 30.377807504232297 21.10357288798302 36-37 21.055404890971904 28.123600780737178 29.81197908677597 21.00901524151495 38-39 20.99303464513019 28.263148048528485 29.893784742356388 20.850032563984932 40-41 20.840056092341207 27.994469016930797 30.022227024932274 21.14324786579572 42-43 20.822871542796427 27.89863594241967 29.90205233511201 21.37644017967189 44-45 20.99154200486512 28.05751765814709 29.763818547043087 21.1871217899447 46-47 21.126467579361442 28.377330313440645 29.394619147340855 21.10158295985706 48-49 21.089571403912043 28.34577967034127 29.242279172984524 21.32236975276216 50-51 21.140151956721812 28.26124311942342 29.144710627331005 21.453894296523764 52-53 21.21928401825974 28.286635463868816 29.07510293958544 21.418977578286007 54-55 21.263268607560278 28.668356910758654 28.902956664703648 21.16541781697742 56-57 21.04760006668163 28.381942937536884 28.972485135553384 21.597971860228103 58-59 20.919942148172822 27.992945777415745 29.42831430342656 21.65879777098487 60-61 21.156760312435054 28.43621205637465 29.028449793354728 21.37857783783557 62-63 20.990577935173498 28.385182913285746 29.088217366692522 21.536021784848238 64-65 21.00598195649822 28.12871505202683 29.23091991187949 21.634383079595455 66-67 20.91654008902498 28.230803115346614 29.0538449336193 21.798811862009106 68-69 21.397874230092132 28.308892577499893 28.962800113121084 21.33043307928689 70-71 21.295230059566634 28.188068948101115 28.93156146777571 21.58513952455655 72-73 21.12134510253557 28.328861340703753 28.994421740853316 21.55537181590736 74-75 21.18834560978275 28.380918026761503 28.852491382478867 21.578244980976883 76-77 21.196065938142443 28.40327160718984 28.738379298757277 21.662283155910444 78-79 21.290139628719444 28.4675018924434 28.849265582954054 21.393092895883097 80-81 21.264116796115903 28.213414293430684 28.913123110258464 21.60934580019495 82-83 21.087620233163477 28.389089029582365 29.01005635844655 21.51323437880761 84-85 21.032125321254767 28.304100792431008 28.846652322797283 21.817121563516945 86-87 21.21383515037127 28.38807211860829 28.733842228127248 21.664250502893186 88-89 20.9885683571144 28.254741661571774 28.817763771295123 21.938926210018703 90-91 20.929785687189845 28.195197316914626 28.90618451923811 21.96883247665742 92-93 21.0480895371715 28.442328502877352 28.680007177079535 21.829574782871614 94-95 20.810100435653695 28.215266442869268 28.869864523310916 22.104768598166117 96-97 20.78771862619771 28.201002307716667 28.863578370169673 22.147700695915955 98-99 20.792607856418673 28.265478061170324 28.80372798371871 22.138186098692294 100 20.931430951962614 28.1262358887503 28.60711884337266 22.335214315914428 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 39.0 1 32.0 2 133.5 3 248.5 4 180.5 5 126.0 6 296.5 7 696.5 8 1239.0 9 1542.5 10 1274.5 11 971.5 12 1057.0 13 1288.0 14 1472.0 15 1686.0 16 1956.5 17 2232.5 18 2623.0 19 3209.0 20 4022.0 21 5143.5 22 6907.0 23 9787.5 24 13404.5 25 18146.5 26 24530.0 27 32383.5 28 42007.0 29 52071.0 30 61827.0 31 72214.5 32 81994.5 33 91584.5 34 100797.0 35 107182.0 36 112124.0 37 117552.0 38 121278.0 39 121593.0 40 121292.5 41 120878.0 42 118771.5 43 116344.5 44 113278.0 45 109624.5 46 106594.5 47 102797.0 48 98794.5 49 95379.0 50 91561.5 51 87590.0 52 81422.0 53 75780.5 54 72472.0 55 68518.0 56 63415.5 57 57665.0 58 52113.0 59 46585.0 60 40640.5 61 33914.5 62 28235.0 63 23307.5 64 18500.0 65 14594.5 66 11274.0 67 8496.5 68 6497.5 69 4964.5 70 3541.5 71 2405.0 72 1680.5 73 1189.5 74 883.0 75 632.0 76 370.0 77 213.5 78 145.5 79 86.5 80 43.0 81 18.5 82 8.0 83 4.5 84 5.5 85 3.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09402843390040126 2 0.0010554528730979341 3 0.0 4 1.5521365780851975E-4 5 0.03836881621026608 6 1.8625638937022369E-4 7 0.004252854223953441 8 2.7938458405533554E-4 9 1.8625638937022369E-4 10-11 3.104273156170395E-4 12-13 0.0 14-15 6.20854631234079E-5 16-17 0.0 18-19 1.0864956046596381E-4 20-21 0.0 22-23 2.6386321827448353E-4 24-25 4.656409734255592E-5 26-27 0.0015210938465234935 28-29 0.008381537521660067 30-31 0.03448847476505308 32-33 0.02452375793374612 34-35 0.027628031089916513 36-37 0.02545503988059724 38-39 0.024321980178595045 40-41 0.029863107762359196 42-43 0.022273159895522584 44-45 0.021279792485548055 46-47 0.015552408512413678 48-49 0.010492443267855934 50-51 0.0078072469877685435 52-53 0.0056032130468875625 54-55 0.004190768760830034 56-57 0.0027628031089916515 58-59 0.0010244101415362303 60-61 7.605469232617468E-4 62-63 0.0013814015544958258 64-65 0.002964580864142727 66-67 7.139828259191908E-4 68-69 0.001210666530906454 70-71 4.811623392064112E-4 72-73 6.363759970149309E-4 74-75 6.829400943574868E-4 76-77 4.190768760830033E-4 78-79 4.656409734255592E-5 80-81 8.071110206043027E-4 82-83 6.518973627957829E-4 84-85 4.0355551030215133E-4 86-87 1.8625638937022369E-4 88-89 1.5521365780851974E-5 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3221366.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.25990451539808 #Duplication Level Percentage of deduplicated Percentage of total 1 75.07227969206463 48.241375247653316 2 15.294950596063764 19.65704129741577 3 4.76452868792299 9.185044756404187 4 1.9801390723741055 5.089741908718758 5 0.9806477194318881 3.150816440696801 6 0.5530433914341648 2.1323109315858813 7 0.3374950697229812 1.5181180668371475 8 0.21589186790253273 1.1098552653654152 9 0.14193340114954828 0.82085641248741 >10 0.6256759130148091 6.6598819131704285 >50 0.025289009397835235 1.10788101534372 >100 0.007690466631422895 0.8226727073436403 >500 2.417293853406631E-4 0.09056034682692479 >1k 1.450376279732993E-4 0.25089720303703283 >5k 4.834587599109977E-5 0.1629464871135757 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5245 0.1628191270411372 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4465 0.13860579642300813 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.5521365780851974E-5 0.0 12-13 0.0 0.0 0.0 3.104273156170395E-5 0.0 14-15 0.0 0.0 0.0 3.104273156170395E-5 0.0 16-17 0.0 0.0 0.0 3.104273156170395E-5 0.0 18-19 0.0 0.0 0.0 6.20854631234079E-5 0.0 20-21 0.0 0.0 0.0 1.241709262468158E-4 0.0 22-23 0.0 0.0 0.0 1.5521365780851975E-4 0.0 24-25 0.0 0.0 0.0 2.3282048671277963E-4 0.0 26-27 0.0 0.0 0.0 4.1907687608300334E-4 0.0 28-29 0.0 0.0 0.0 0.0011485810677830462 0.0 30-31 0.0 0.0 0.0 0.003600956861157658 0.0 32-33 0.0 0.0 0.0 0.010507964633636787 0.0 34-35 0.0 0.0 0.0 0.020767587414779944 0.0 36-37 0.0 0.0 0.0 0.03897414947571931 0.0 38-39 0.0 0.0 0.0 0.07102576981317864 0.0 40-41 0.0 0.0 0.0 0.10841673997925104 0.0 42-43 0.0 0.0 0.0 0.14822904320713634 0.0 44-45 0.0 0.0 0.0 0.19296161938755174 0.0 46-47 0.0 0.0 0.0 0.2507942282870062 0.0 48-49 0.0 0.0 0.0 0.3154717594958164 0.0 50-51 0.0 0.0 0.0 0.3841538030760864 0.0 52-53 0.0 0.0 0.0 0.4532704448982202 0.0 54-55 0.0 0.0 0.0 0.5292630517612715 0.0 56-57 0.0 0.0 0.0 0.6193645801191172 0.0 58-59 0.0 0.0 0.0 0.7188099706770358 0.0 60-61 0.0 0.0 0.0 0.83129330849087 0.0 62-63 0.0 0.0 0.0 0.943497261720649 0.0 64-65 0.0 0.0 0.0 1.060823265658109 0.0 66-67 0.0 0.0 0.0 1.1922892338219253 0.0 68-69 0.0 0.0 0.0 1.3457800200287704 0.0 70-71 0.0 0.0 0.0 1.5120448902732568 0.0 72-73 0.0 0.0 0.0 1.6841147513197816 0.0 74-75 0.0 0.0 0.0 1.8579850907968853 0.0 76-77 0.0 0.0 0.0 2.047190539665471 0.0 78-79 0.0 0.0 0.0 2.2706671641781777 0.0 80-81 0.0 0.0 0.0 2.514352606937554 0.0 82-83 0.0 0.0 0.0 2.7713709029026816 0.0 84-85 0.0 0.0 0.0 3.020147353638177 0.0 86-87 0.0 0.0 0.0 3.2865560759007204 0.0 88 0.0 0.0 0.0 3.513074888106474 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4820 0.0 44.317757 1 GTATCAA 8190 0.0 39.123005 1 TCAACGC 9225 0.0 33.63168 4 ATCAACG 9250 0.0 33.54078 3 CAACGCA 9375 0.0 33.09357 5 AACGCAG 9835 0.0 31.728043 6 TATCAAC 9985 0.0 31.495535 2 ACGCAGA 10955 0.0 28.31225 7 CGCAGAG 11160 0.0 27.79218 8 GTACATG 7595 0.0 25.77119 1 TACATGG 7610 0.0 24.337868 2 GCAGAGT 12855 0.0 23.798618 9 ACATGGG 7790 0.0 22.930689 3 GAGTACT 8525 0.0 21.195557 12-13 TATACCG 160 5.4097327E-5 20.565962 5 AGAGTAC 11230 0.0 20.295736 10-11 GAGTACA 5710 0.0 20.270662 1 CAGAGTA 12560 0.0 19.736752 10-11 CATGGGG 5240 0.0 19.556726 4 AGTATCG 175 1.0770933E-4 18.803164 5 >>END_MODULE