FastQCFastQC Report
Wed 25 May 2016
SRR1033159_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033159_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762144
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13800.034.7944151
TCAACGC18100.034.777754
ATCAACG18700.033.662843
CAACGCA19250.033.188185
AACGCAG20300.031.9289286
GTATCAA21800.030.447461
GTGTACG1853.2196112E-1027.990421
GTACATG43600.027.9641571
TATCAAC23900.026.7470072
TACATGG43750.025.7850152
ACGCAGA24500.025.496877
CGCAGAG25500.024.3128058
ACATGGG44050.024.2084833
CTAGTAC4550.021.6818073
TAGACCG1150.003971231620.4243135
TATGTCG2601.6942067E-819.8744265
GTACGGT1250.006437825518.7871676
TATACTG11050.018.705345
GCAGAGT31400.018.547849
GAGTACA31050.018.193161
Produced by FastQC (version 0.11.5)