##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033159_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1762144
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.44018706757223	33.0	31.0	34.0	30.0	34.0
2	31.537580356656438	34.0	31.0	34.0	30.0	34.0
3	31.69605321699021	34.0	31.0	34.0	30.0	34.0
4	35.06766643361723	37.0	35.0	37.0	33.0	37.0
5	35.14443314507781	37.0	35.0	37.0	33.0	37.0
6	35.28679154484537	37.0	36.0	37.0	33.0	37.0
7	35.176950351390126	37.0	35.0	37.0	33.0	37.0
8	35.18954466831315	37.0	35.0	37.0	33.0	37.0
9	36.84726390124757	39.0	38.0	39.0	34.0	39.0
10-11	36.835483933208636	39.0	38.0	39.0	34.0	39.0
12-13	36.79016442470082	39.0	38.0	39.0	33.0	39.0
14-15	38.22175372727768	41.0	39.0	41.0	34.0	41.0
16-17	38.228532401438244	41.0	39.0	41.0	34.0	41.0
18-19	38.20575162983275	41.0	39.0	41.0	33.5	41.0
20-21	38.247953912960575	41.0	39.0	41.0	34.0	41.0
22-23	38.19902090862041	40.5	39.0	41.0	34.0	41.0
24-25	38.191743694045435	40.5	39.0	41.0	33.5	41.0
26-27	38.155559364047434	40.0	39.0	41.0	33.0	41.0
28-29	38.122874464288955	40.0	38.5	41.0	33.0	41.0
30-31	38.12343514491438	40.0	38.0	41.0	33.0	41.0
32-33	38.19726651170392	41.0	39.0	41.0	33.0	41.0
34-35	38.171627857882214	41.0	39.0	41.0	33.0	41.0
36-37	38.08891242713422	41.0	38.0	41.0	33.0	41.0
38-39	38.008484834383566	41.0	38.0	41.0	33.0	41.0
40-41	37.94925045853233	40.5	38.0	41.0	33.0	41.0
42-43	37.83526289565439	40.0	38.0	41.0	33.0	41.0
44-45	37.69873971707193	40.0	38.0	41.0	32.0	41.0
46-47	37.5783187980097	40.0	38.0	41.0	32.0	41.0
48-49	37.46548919952058	40.0	38.0	41.0	32.0	41.0
50-51	37.34856742695262	40.0	37.5	41.0	31.5	41.0
52-53	37.204268777126046	40.0	37.0	41.0	31.0	41.0
54-55	37.06184795340222	40.0	37.0	41.0	31.0	41.0
56-57	36.90991712368569	40.0	36.5	41.0	31.0	41.0
58-59	36.72484655056567	40.0	36.0	41.0	31.0	41.0
60-61	36.51467530462891	39.5	35.5	41.0	30.5	41.0
62-63	36.26724632039152	39.0	35.0	41.0	30.0	41.0
64-65	36.003816941180744	39.0	35.0	41.0	30.0	41.0
66-67	35.722225879383295	38.5	35.0	40.5	29.5	41.0
68-69	35.42658573873645	37.5	35.0	40.0	29.0	41.0
70-71	35.09009365863403	37.0	35.0	40.0	29.0	41.0
72-73	34.73285639539107	37.0	34.0	39.0	29.0	41.0
74-75	34.366121610946664	36.0	34.0	39.0	28.5	41.0
76-77	34.03452640646849	36.0	34.0	39.0	28.0	40.0
78-79	33.66099138322407	35.0	34.0	37.5	28.0	39.5
80-81	33.29189612199684	35.0	34.0	37.0	27.5	39.0
82-83	33.05647835818185	35.0	34.0	37.0	27.5	39.0
84-85	32.77109362231464	35.0	34.0	36.0	27.0	38.0
86-87	32.49784580601812	35.0	34.0	36.0	27.0	37.0
88-89	32.11708407485427	35.0	33.5	36.0	26.0	37.0
90-91	31.91355104917646	35.0	33.0	35.0	26.0	36.5
92-93	31.721651011495087	35.0	33.0	35.0	25.5	36.0
94-95	31.54159421704469	35.0	33.0	35.0	25.0	36.0
96-97	31.355845776508616	35.0	33.0	35.0	25.0	36.0
98-99	31.156263619772275	35.0	33.0	35.0	24.0	35.5
100	30.99121070695698	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.02022137200312457
1101	2	0.05254983198122787
1101	3	0.04980337459288364
1101	4	0.05296893447923168
1101	5	0.042553335417096605
1101	6	0.09087820751278741
1101	7	0.1569930481233115
1101	8	0.08435678810705127
1101	9	0.11703750690263348
1101	10-11	0.09060611504531124
1101	12-13	0.16146079635898758
1101	14-15	0.16326229466970688
1101	16-17	0.0630511819162507
1101	18-19	0.19930241596877352
1101	20-21	0.09822267267487206
1101	22-23	0.16419574066378573
1101	24-25	0.07700905953205961
1101	26-27	0.12476376181653137
1101	28-29	0.1855360106849986
1101	30-31	0.21190603493342053
1101	32-33	0.2119685519449348
1101	34-35	0.2984908633073218
1101	36-37	0.2565161382850576
1101	38-39	0.23162744796950818
1101	40-41	0.2705588170779549
1101	42-43	0.21184904336242028
1101	44-45	0.1200842539066116
1101	46-47	0.1723398415850994
1101	48-49	0.08952865916436537
1101	50-51	0.19420219053736787
1101	52-53	0.16072127351279164
1101	54-55	0.1157226988182174
1101	56-57	0.08827948055094481
1101	58-59	0.12631631300872925
1101	60-61	0.11421940779378303
1101	62-63	0.21027068089753698
1101	64-65	0.06190808759616573
1101	66-67	0.1860151549297413
1101	68-69	0.13988624132601757
1101	70-71	0.1464399764090203
1101	72-73	0.14299024047024744
1101	74-75	0.18902241264176922
1101	76-77	0.1640754306783947
1101	78-79	0.13386086766036698
1101	80-81	0.20799119649308295
1101	82-83	0.14230523918389082
1101	84-85	0.08626018345803033
1101	86-87	0.14171770692404806
1101	88-89	0.3023265832831541
1101	90-91	0.3484344498866676
1101	92-93	0.32172350087699186
1101	94-95	0.3193238341898592
1101	96-97	0.3268199071069766
1101	98-99	0.299173438308447
1101	100	0.31085746185097207
1102	1	0.008941503423397279
1102	2	0.02915395988707914
1102	3	0.039868799404857214
1102	4	0.056884945143195864
1102	5	-0.004931389009293241
1102	6	0.10488293473339638
1102	7	0.051356360641001686
1102	8	0.04050619241625242
1102	9	0.07893507242009434
1102	10-11	0.07881052880141226
1102	12-13	0.04213733922389906
1102	14-15	0.13673464711952477
1102	16-17	0.06743617589326334
1102	18-19	0.024029085606755984
1102	20-21	0.06260207820736952
1102	22-23	0.06110517342682442
1102	24-25	0.05499176880602619
1102	26-27	0.12254347018761536
1102	28-29	0.0568137016079433
1102	30-31	0.0858147058445482
1102	32-33	0.0714300771114722
1102	34-35	0.09294367620691446
1102	36-37	0.1436052080550141
1102	38-39	0.125797755816059
1102	40-41	0.21122660668088855
1102	42-43	0.14248936300570847
1102	44-45	0.06492099360021086
1102	46-47	-0.011188906928005338
1102	48-49	0.03928054075493037
1102	50-51	0.01589309511435033
1102	52-53	-0.055492660276598826
1102	54-55	-0.006426546758177665
1102	56-57	-0.05939631358047137
1102	58-59	-0.024706726534432732
1102	60-61	-0.08728189634058481
1102	62-63	0.007096614953240987
1102	64-65	0.02838307783114402
1102	66-67	0.043284347087947594
1102	68-69	0.031739837198180965
1102	70-71	0.10959420403121811
1102	72-73	0.14296670931450706
1102	74-75	0.11841369525865986
1102	76-77	0.05042396595823817
1102	78-79	0.06510660212595099
1102	80-81	0.05053062306751599
1102	82-83	0.13543373175673423
1102	84-85	0.12080441202803627
1102	86-87	0.11195694416696256
1102	88-89	0.19437966104354132
1102	90-91	0.2437608999879899
1102	92-93	0.33453969975739284
1102	94-95	0.30841406887501677
1102	96-97	0.19978298631366798
1102	98-99	0.27357162285108316
1102	100	0.2623129335252017
1103	1	0.024049536789529924
1103	2	-0.04035075582605785
1103	3	0.011005396500955555
1103	4	0.06604994245345353
1103	5	-0.08881706537461298
1103	6	0.02395660814779177
1103	7	-0.00548303788806237
1103	8	0.02656376588623033
1103	9	-0.013765187519140909
1103	10-11	-0.006836123721456033
1103	12-13	0.039722458903789004
1103	14-15	0.04416982853140894
1103	16-17	0.07270797002113483
1103	18-19	0.08641442173831138
1103	20-21	0.024160752864041513
1103	22-23	0.0758091891396191
1103	24-25	0.11099052430439826
1103	26-27	0.12450383064771131
1103	28-29	0.1679857600015353
1103	30-31	0.13967487183536065
1103	32-33	0.16337940502651804
1103	34-35	0.2048143352042473
1103	36-37	0.2316451084567106
1103	38-39	0.14045309853596422
1103	40-41	0.17945960525219817
1103	42-43	0.22557225311258833
1103	44-45	0.1273958816761862
1103	46-47	0.14355828459258646
1103	48-49	0.16391553307382623
1103	50-51	0.12254627968010823
1103	52-53	0.1131691219120654
1103	54-55	0.2123910844449597
1103	56-57	0.1369110127865767
1103	58-59	0.13570514242260145
1103	60-61	0.1462570883407821
1103	62-63	0.19300653110525445
1103	64-65	0.15891330805440163
1103	66-67	0.11935220233001331
1103	68-69	0.18422834734968063
1103	70-71	0.19746788457160136
1103	72-73	0.1697920499379748
1103	74-75	0.11613202942070444
1103	76-77	0.1514185969771873
1103	78-79	0.17942331760877295
1103	80-81	0.1741791124391412
1103	82-83	0.13157870347072986
1103	84-85	0.128065913567589
1103	86-87	0.23221613898841298
1103	88-89	0.29814793687752683
1103	90-91	0.2884017489722339
1103	92-93	0.2723441246732605
1103	94-95	0.3856375895739177
1103	96-97	0.3149968118613877
1103	98-99	0.3885942557927713
1103	100	0.5225461121547674
1104	1	0.00890247715684822
1104	2	-0.009309860110043644
1104	3	-0.03313389090985552
1104	4	0.011254341556707459
1104	5	-0.19367053151167823
1104	6	-0.09072233300084065
1104	7	-0.1844425273289545
1104	8	-0.1641423979081651
1104	9	-0.20692749080636474
1104	10-11	-0.12312003816282413
1104	12-13	-0.11004504082262656
1104	14-15	-0.17725687824864167
1104	16-17	-0.15766553436262143
1104	18-19	-0.14931154095526722
1104	20-21	-0.18030193185079213
1104	22-23	-0.2241734787995
1104	24-25	-0.19941109653368017
1104	26-27	-0.11605157172815694
1104	28-29	-0.09849803864820927
1104	30-31	-0.1339202683403542
1104	32-33	-0.17759832495648453
1104	34-35	-0.31103594146679825
1104	36-37	-0.23575258194682647
1104	38-39	-0.26166844353448226
1104	40-41	-0.3114503860236937
1104	42-43	-0.18397807925337872
1104	44-45	-0.24973600766517734
1104	46-47	-0.19255286557764606
1104	48-49	-0.24160211475458482
1104	50-51	-0.2604710212014112
1104	52-53	-0.3538515121051091
1104	54-55	-0.293279128314488
1104	56-57	-0.3313829316297827
1104	58-59	-0.32075592585593427
1104	60-61	-0.4091980173419927
1104	62-63	-0.49534650738536357
1104	64-65	-0.5245477791762951
1104	66-67	-0.4768561724484428
1104	68-69	-0.4280690402471663
1104	70-71	-0.38341353784343113
1104	72-73	-0.3976883574969037
1104	74-75	-0.4189381883519019
1104	76-77	-0.48328575230021187
1104	78-79	-0.5047380826994825
1104	80-81	-0.4415272194792905
1104	82-83	-0.46757719153917066
1104	84-85	-0.40092830113109557
1104	86-87	-0.30490641488312065
1104	88-89	-0.31313367579279117
1104	90-91	-0.23738863274272148
1104	92-93	-0.26200285401533563
1104	94-95	-0.18082475033377676
1104	96-97	-0.17423499574112
1104	98-99	-0.0971867293163271
1104	100	-0.043947254281683
1105	1	0.03605882584301767
1105	2	0.15381383648770708
1105	3	0.16289750991240481
1105	4	0.22709298780588938
1105	5	0.18767100456649644
1105	6	0.16198010398896656
1105	7	0.08356468234750736
1105	8	0.12514855126659796
1105	9	0.14391770969414353
1105	10-11	0.23988523503028603
1105	12-13	0.24081877971006094
1105	14-15	0.20171976478299314
1105	16-17	0.24498834158327298
1105	18-19	0.25821303748720936
1105	20-21	0.2712208888606398
1105	22-23	0.2979915095816068
1105	24-25	0.14147000338885363
1105	26-27	0.1719675547688908
1105	28-29	0.22917937301835423
1105	30-31	0.23019654629349873
1105	32-33	0.29857414122953685
1105	34-35	0.30627204876141434
1105	36-37	0.3204417813970295
1105	38-39	0.26511386594534514
1105	40-41	0.19990068351095402
1105	42-43	0.07770934254249084
1105	44-45	0.13045838547073885
1105	46-47	0.14345112655725245
1105	48-49	0.13403513722657578
1105	50-51	0.14302909796680296
1105	52-53	0.14933161963534047
1105	54-55	0.07875909488755894
1105	56-57	0.1371761758003558
1105	58-59	0.1314547933241883
1105	60-61	0.09551016951375857
1105	62-63	0.23893907922013113
1105	64-65	0.23924206769255107
1105	66-67	0.16526517057723566
1105	68-69	0.0990134241513303
1105	70-71	-0.07109914232415804
1105	72-73	0.0445238512857955
1105	74-75	0.07576835994101572
1105	76-77	0.08474201389176272
1105	78-79	0.10663235166862961
1105	80-81	0.19257857209550622
1105	82-83	0.12704862029423225
1105	84-85	0.07248929405451321
1105	86-87	0.11856927480287283
1105	88-89	0.18398349634568234
1105	90-91	0.18888259409396113
1105	92-93	0.17383340886972576
1105	94-95	0.24163935024418848
1105	96-97	0.2847486484226174
1105	98-99	0.24954850872216383
1105	100	0.22819300669668863
1106	1	0.14356060712953322
1106	2	0.012472456489415862
1106	3	0.06576595121545381
1106	4	-0.009059433318739707
1106	5	-0.05466647085174969
1106	6	-0.07843215696237849
1106	7	-0.14856929889911186
1106	8	-0.0954069251206704
1106	9	0.006590066977466336
1106	10-11	-0.027421958517180656
1106	12-13	-0.05424712789105257
1106	14-15	-0.014064856301736484
1106	16-17	-0.08760236152613743
1106	18-19	-0.030132076087625137
1106	20-21	-0.04377519230902038
1106	22-23	-0.013785074338358072
1106	24-25	0.03813636129919473
1106	26-27	0.02141535594881816
1106	28-29	0.06646982247256261
1106	30-31	0.05525133866674281
1106	32-33	0.04500807687522013
1106	34-35	-0.051308251634750945
1106	36-37	0.02385643639745183
1106	38-39	-0.03366880284936258
1106	40-41	-0.016125052516635208
1106	42-43	0.0643610939033934
1106	44-45	0.0727112854051839
1106	46-47	0.00917646642671599
1106	48-49	0.06472228631314181
1106	50-51	0.09230853194519284
1106	52-53	-0.014913043630258471
1106	54-55	0.04766046861572448
1106	56-57	-0.04548114742476628
1106	58-59	0.01718818460870608
1106	60-61	0.03441752836461376
1106	62-63	0.05084705083354635
1106	64-65	-2.6159463252639625E-4
1106	66-67	0.0665305252670052
1106	68-69	0.07237321552705822
1106	70-71	0.1322823807470641
1106	72-73	0.1414507045362825
1106	74-75	0.12349999843960546
1106	76-77	0.1172771408618658
1106	78-79	0.09998972049469046
1106	80-81	0.12984657733962024
1106	82-83	0.17346623935542738
1106	84-85	0.1445542326357483
1106	86-87	0.08051009333965453
1106	88-89	0.12074191114318467
1106	90-91	0.16256828965072145
1106	92-93	0.1496803037310741
1106	94-95	0.19149584980242906
1106	96-97	0.11469556608443199
1106	98-99	0.1593251353930114
1106	100	0.24344452435279962
1107	1	0.06427198171271442
1107	2	0.03803748057240952
1107	3	0.009078289118381377
1107	4	0.03577198572534712
1107	5	0.033514336854807425
1107	6	-0.011370282193944092
1107	7	-0.004441844286290575
1107	8	0.02797210414942697
1107	9	0.0658712822423837
1107	10-11	0.0768759295895336
1107	12-13	0.03726824817416485
1107	14-15	0.007424584231799258
1107	16-17	0.0817196095742645
1107	18-19	0.10732624180789685
1107	20-21	0.0649687869425506
1107	22-23	0.07217268779575647
1107	24-25	0.06370138538218839
1107	26-27	0.14423862382580666
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2216	8	-0.010941478677935379
2216	9	-0.069066188985353
2216	10-11	-0.04139685715958308
2216	12-13	-0.04610908342871767
2216	14-15	-0.15558989110036237
2216	16-17	-0.07727762474140576
2216	18-19	-0.11677812642227536
2216	20-21	-0.2260093649315138
2216	22-23	-0.1305375267936313
2216	24-25	-0.11371027740246831
2216	26-27	-0.27360580932712253
2216	28-29	-0.1568434044594298
2216	30-31	-0.17925721149943996
2216	32-33	-0.16999846198091006
2216	34-35	-0.19541466274023378
2216	36-37	-0.2180231933219261
2216	38-39	-0.22217382243518813
2216	40-41	-0.21320790222367236
2216	42-43	-0.24331318948833314
2216	44-45	-0.14906606104637632
2216	46-47	-0.05761828584272877
2216	48-49	-0.022932611043017914
2216	50-51	0.014813865528523706
2216	52-53	-0.055414465485661424
2216	54-55	-0.07374679583945465
2216	56-57	-0.08618632481325506
2216	58-59	-0.1191707282506016
2216	60-61	-0.14237199140126933
2216	62-63	-0.07281807555872888
2216	64-65	3.438790911118872E-4
2216	66-67	-0.05753844731093949
2216	68-69	-0.042765329959905785
2216	70-71	-0.15174969948875372
2216	72-73	-0.017167155537634926
2216	74-75	-0.05924808500522971
2216	76-77	-0.12892054562912136
2216	78-79	-0.10202505346612156
2216	80-81	-0.13881446211978954
2216	82-83	-0.051017503116497664
2216	84-85	-0.17044661974451714
2216	86-87	-0.17473358219973534
2216	88-89	-0.08303076412262556
2216	90-91	-0.07635082596396359
2216	92-93	-0.07060378125348876
2216	94-95	-0.12563299770141967
2216	96-97	-0.17525736502787836
2216	98-99	-0.21672842908157364
2216	100	-0.1759457199244885
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	22775.0
3	3675.0
4	2429.0
5	1919.0
6	1702.0
7	1697.0
8	1852.0
9	2163.0
10	2456.0
11	2834.0
12	2977.0
13	3324.0
14	3783.0
15	3812.0
16	3814.0
17	3864.0
18	3712.0
19	3839.0
20	3908.0
21	4134.0
22	4609.0
23	6120.0
24	8889.0
25	12216.0
26	13307.0
27	12256.0
28	12839.0
29	15529.0
30	19907.0
31	23508.0
32	30993.0
33	42037.0
34	58381.0
35	87816.0
36	159184.0
37	339067.0
38	568547.0
39	265579.0
40	691.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.1665256548509	19.801930590302412	13.175817631297562	23.855726123549125
2	15.128886494999822	19.786345400693282	41.30725660880993	23.77751149549697
3	18.389536376005395	25.101760594572614	29.71000501988647	26.798698009535514
4	11.783226509676577	16.403969411911643	39.4777657297598	32.33503834865198
5	12.40042716045598	38.306482517589714	36.128611404911666	13.164478917042647
6	31.017558157815134	38.57210070755908	17.777514184383076	12.632826950242713
7	27.29863236978342	32.34316633239237	22.570949577361514	17.78725172046269
8	24.721304478369277	37.59819827694341	20.11920296003846	17.56129428464886
9	24.501869355678192	17.085292226238863	21.22869482250628	37.184143595576664
10-11	23.63289969181833	27.270601330298156	29.16555392242699	19.930945055456526
12-13	24.111388815655232	24.396796688962176	29.34788685509016	22.14392764029243
14-15	21.224025180686066	26.682536560779397	26.165771714751706	25.92766654378283
16-17	21.17821678212525	30.203171161557822	26.676410406313945	21.94220165000298
18-19	21.019484085371822	29.225021249302923	28.637334351218698	21.118160314106557
20-21	22.164384634683785	28.543440723741497	28.183965343235702	21.108209298339023
22-23	21.659275849366953	28.318219665344397	28.294750296701633	21.72775418858702
24-25	21.600063347039605	28.417561434140648	28.23387772432004	21.748497494499706
26-27	21.40540325773389	28.313762996241078	28.35380709867565	21.927026647349386
28-29	21.36682084514173	28.389708090141337	28.4267722247165	21.81669884000043
30-31	21.60383880558384	28.87626731327882	28.04719950868374	21.472694372453596
32-33	21.27666216916546	28.570702819235077	28.49472588988369	21.65790912171578
34-35	21.294764112484984	28.328580384059492	28.611742457028356	21.76491304642717
36-37	21.63310825478759	28.709305311787464	28.004670888249137	21.652915545175805
38-39	21.53449907118213	28.851727975835413	28.139378869719746	21.47439408326272
40-41	21.27228422789015	28.540979880310818	28.492029281822358	21.69470660997667
42-43	21.314068411716615	28.433561190776242	28.474736144032313	21.77763425347483
44-45	21.397691069359578	28.58163621757172	28.44236143961539	21.578311273453313
46-47	21.427813362300384	28.77315122230281	28.30388387754384	21.495151537852966
48-49	21.349640295118892	28.56107548538806	28.37220665813692	21.71707756135612
50-51	21.314601923972415	28.482663334906327	28.457039502415256	21.745695238706006
52-53	21.470449106834515	28.38700139243635	28.407120352527315	21.73542914820182
54-55	21.5076640806182	28.864636664494046	28.198578557713073	21.42912069717468
56-57	21.324618316007815	28.623554098323446	28.227803023961567	21.824024561707176
58-59	21.22025631960102	28.228298442140243	28.67214460951923	21.879300628739507
60-61	21.4464276998966	28.687873350108905	28.264397991925478	21.601300958069018
62-63	21.359846455584712	28.62088671672826	28.281007391197754	21.738259436489273
64-65	21.274050432309775	28.343126074531906	28.537297305579777	21.845526187578546
66-67	21.204341276374297	28.488739484543657	28.271130863962124	22.035788375119918
68-69	21.602812569165025	28.536936251837307	28.272761323201422	21.587489855796242
70-71	21.502296262561345	28.361687245775997	28.31798944726541	21.818027044397247
72-73	21.287354730225335	28.499193722070054	28.487276188000806	21.72617535970381
74-75	21.36071010645241	28.51012597454631	28.321970010816628	21.807193908184658
76-77	21.348932815835038	28.567387051684186	28.22503344083965	21.858646691641123
78-79	21.509901783771067	28.586106828770408	28.250963420250425	21.6530279672081
80-81	21.434595157197833	28.344642890585497	28.42948585165591	21.791276100560758
82-83	21.21280995722145	28.51421998095452	28.533515162039798	21.739454899784235
84-85	21.29538145305776	28.362865481975675	28.413827662703007	21.92792540226356
86-87	21.46053212151995	28.520555758123482	28.244719716133755	21.77419240422281
88-89	21.265953811629622	28.379536072335647	28.349019398498942	22.005490717535782
90-91	21.188750076014156	28.298422017468557	28.50760726846659	22.005220638050705
92-93	21.349684949609923	28.53136643453629	28.357247243826535	21.761701372027247
94-95	21.163025563178337	28.25968060442299	28.467944835098457	22.109348997300216
96-97	21.159305284581272	28.290771143249017	28.466662194699005	22.083261377470702
98-99	21.130497380256188	28.387313338258082	28.35956882978687	22.122620451698864
100	21.322815388488607	28.189279620111744	28.31173398231291	22.176171009086744
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	252.0
1	238.5
2	213.5
3	191.5
4	154.0
5	126.0
6	113.5
7	100.0
8	117.5
9	123.5
10	115.0
11	116.0
12	125.0
13	146.5
14	178.0
15	220.0
16	297.5
17	436.5
18	617.5
19	887.0
20	1365.5
21	2019.0
22	2952.5
23	4626.0
24	6778.0
25	9332.5
26	12901.0
27	17102.0
28	22169.0
29	27596.5
30	33021.5
31	38510.5
32	43663.5
33	48737.5
34	53503.0
35	56776.0
36	58965.0
37	62079.5
38	64464.5
39	64743.5
40	64743.0
41	64822.5
42	64208.5
43	63081.5
44	61568.5
45	60141.5
46	58457.0
47	55950.5
48	53929.0
49	52616.0
50	51036.5
51	48943.0
52	45567.5
53	43017.0
54	41642.5
55	39658.0
56	37009.0
57	34183.5
58	31056.0
59	27242.5
60	23621.5
61	20281.0
62	17378.0
63	14421.0
64	11716.0
65	9531.5
66	7356.0
67	5537.0
68	4306.0
69	3444.0
70	2614.0
71	1862.5
72	1466.0
73	1160.5
74	828.5
75	554.5
76	358.5
77	256.5
78	176.0
79	119.0
80	88.0
81	56.5
82	37.0
83	33.0
84	27.0
85	17.5
86	13.5
87	11.0
88	10.5
89	8.5
90	5.0
91	5.0
92	4.5
93	3.0
94	2.5
95	2.0
96	3.0
97	2.5
98	1.5
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3285770061924565
2	0.11957025078540688
3	0.06514791072693266
4	0.038248860479052786
5	0.042278043111119185
6	0.00970408774765286
7	0.006696388036392032
8	0.015605989067862786
9	0.017421958704850454
10-11	0.020344534839377485
12-13	0.01586135979806418
14-15	0.018017823741987037
16-17	0.01867043783027948
18-19	0.01824481994661049
20-21	0.023267110973904517
22-23	0.015492490965551055
24-25	0.023834601485463163
26-27	0.019096055713948464
28-29	0.018897434034902936
30-31	0.019635171699929177
32-33	0.024118346741242486
34-35	0.014499382570323426
36-37	0.009448717017451468
38-39	0.01288203461238128
40-41	0.009278469863983875
42-43	0.008540732198957634
44-45	0.009051473659360416
46-47	0.008058365264132783
48-49	0.004227804311111918
50-51	0.006384268255034776
52-53	0.0070652568689051525
54-55	0.0034333175949298124
56-57	0.0029225761345270307
58-59	0.0026672054043256396
60-61	0.0032914449670401512
62-63	0.0027239544554815043
64-65	0.0022415875206566547
66-67	0.0020145913160331957
68-69	0.0030644487624166923
70-71	0.002355085622968384
72-73	0.001957842264877331
74-75	0.0023834601485463163
76-77	0.00436967693900158
78-79	0.003546815697241542
80-81	0.003688688325131204
82-83	0.002610456353169775
84-85	0.003007699711260828
86-87	0.0026388308787477074
88-89	0.6039517769262898
90-91	0.6143368532878131
92-93	0.6104779178092142
94-95	0.597170265313164
96-97	0.5953826702017542
98-99	0.5928005883741624
100	0.594219314653059
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1762144.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	74.39310521763271
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.56495992901245	61.4226375128865
2	11.659326765135477	17.347470456109694
3	3.057586953070375	6.823901637354761
4	1.1216470400255607	3.337712250626713
5	0.5229583355791729	1.9452247241589744
6	0.29285399813321433	1.3071790977917175
7	0.182406232131064	0.9498836213494724
8	0.11752781239306173	0.6994607130684191
9	0.0763009898842121	0.5108640811799148
>10	0.3905286772438345	4.8204383657185454
>50	0.011690846176146123	0.5819592248866129
>100	0.002212421215450997	0.2532683148686586
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	5.6749051155864675E-5	0.0
6	0.0	0.0	0.0	5.6749051155864675E-5	0.0
7	0.0	0.0	0.0	5.6749051155864675E-5	0.0
8	0.0	0.0	0.0	5.6749051155864675E-5	0.0
9	0.0	0.0	0.0	5.6749051155864675E-5	0.0
10-11	5.6749051155864675E-5	0.0	0.0	5.6749051155864675E-5	0.0
12-13	5.6749051155864675E-5	0.0	0.0	5.6749051155864675E-5	0.0
14-15	5.6749051155864675E-5	0.0	0.0	8.512357673379701E-5	0.0
16-17	5.6749051155864675E-5	0.0	0.0	1.4187262788966168E-4	0.0
18-19	5.6749051155864675E-5	0.0	0.0	1.7024715346759402E-4	0.0
20-21	5.6749051155864675E-5	0.0	0.0	1.7024715346759402E-4	0.0
22-23	5.6749051155864675E-5	0.0	0.0	1.9862167904552636E-4	0.0
24-25	5.6749051155864675E-5	0.0	0.0	3.4049430693518804E-4	0.0
26-27	5.6749051155864675E-5	0.0	0.0	3.6886883251312035E-4	0.0
28-29	5.6749051155864675E-5	0.0	0.0	8.228612417600378E-4	0.0
30-31	5.6749051155864675E-5	0.0	0.0	0.0034049430693518807	0.0
32-33	5.6749051155864675E-5	0.0	0.0	0.008427234096645905	0.0
34-35	5.6749051155864675E-5	0.0	0.0	0.01543574191439519	0.0
36-37	5.6749051155864675E-5	0.0	0.0	0.029850000907984817	0.0
38-39	5.6749051155864675E-5	0.0	0.0	0.05411022027711697	0.0
40-41	5.6749051155864675E-5	0.0	0.0	0.08373322498047833	0.0
42-43	5.6749051155864675E-5	0.0	0.0	0.11440608713022318	0.0
44-45	5.6749051155864675E-5	0.0	0.0	0.14973237147474894	0.0
46-47	5.6749051155864675E-5	0.0	0.0	0.19334401728803097	0.0
48-49	5.6749051155864675E-5	0.0	0.0	0.24351017850981532	0.0
50-51	5.6749051155864675E-5	0.0	0.0	0.29943636842391996	0.0
52-53	5.6749051155864675E-5	0.0	0.0	0.35638404125883016	0.0
54-55	5.6749051155864675E-5	0.0	0.0	0.41563005066555286	0.0
56-57	5.6749051155864675E-5	2.8374525577932338E-5	0.0	0.48503414022917535	0.0
58-59	5.6749051155864675E-5	5.6749051155864675E-5	0.0	0.5655894183449253	0.0
60-61	5.6749051155864675E-5	5.6749051155864675E-5	0.0	0.6535220731109377	0.0
62-63	5.6749051155864675E-5	5.6749051155864675E-5	0.0	0.7440935587556976	0.0
64-65	5.6749051155864675E-5	5.6749051155864675E-5	0.0	0.8396022118510178	0.0
66-67	5.6749051155864675E-5	5.6749051155864675E-5	0.0	0.9475672816750504	0.0
68-69	5.6749051155864675E-5	5.6749051155864675E-5	0.0	1.068527884213776	0.0
70-71	5.6749051155864675E-5	5.6749051155864675E-5	0.0	1.2034771278624221	0.0
72-73	5.6749051155864675E-5	5.6749051155864675E-5	0.0	1.3478467140029418	0.0
74-75	5.6749051155864675E-5	5.6749051155864675E-5	0.0	1.4963022318266839	0.0
76-77	5.6749051155864675E-5	5.6749051155864675E-5	0.0	1.649297673742895	0.0
78-79	5.6749051155864675E-5	5.6749051155864675E-5	0.0	1.823772631521601	0.0
80-81	5.6749051155864675E-5	5.6749051155864675E-5	0.0	2.0206918390324513	0.0
82-83	5.6749051155864675E-5	5.6749051155864675E-5	0.0	2.2266908947282404	0.0
84-85	5.6749051155864675E-5	5.6749051155864675E-5	0.0	2.4260786859643706	5.6749051155864675E-5
86-87	5.6749051155864675E-5	5.6749051155864675E-5	0.0	2.64620825539797	5.6749051155864675E-5
88	5.6749051155864675E-5	5.6749051155864675E-5	0.0	2.8240030326692938	5.6749051155864675E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1380	0.0	34.794415	1
TCAACGC	1810	0.0	34.77775	4
ATCAACG	1870	0.0	33.66284	3
CAACGCA	1925	0.0	33.18818	5
AACGCAG	2030	0.0	31.928928	6
GTATCAA	2180	0.0	30.44746	1
GTGTACG	185	3.2196112E-10	27.99042	1
GTACATG	4360	0.0	27.964157	1
TATCAAC	2390	0.0	26.747007	2
TACATGG	4375	0.0	25.785015	2
ACGCAGA	2450	0.0	25.49687	7
CGCAGAG	2550	0.0	24.312805	8
ACATGGG	4405	0.0	24.208483	3
CTAGTAC	455	0.0	21.681807	3
TAGACCG	115	0.0039712316	20.424313	5
TATGTCG	260	1.6942067E-8	19.874426	5
GTACGGT	125	0.0064378255	18.787167	6
TATACTG	1105	0.0	18.70534	5
GCAGAGT	3140	0.0	18.54784	9
GAGTACA	3105	0.0	18.19316	1
>>END_MODULE