FastQCFastQC Report
Wed 25 May 2016
SRR1033159_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033159_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762144
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30500.17308460602538725No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24730.14034040350845334No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23700.052.6033941
GTATCAA42700.041.0957761
TCAACGC50350.034.080134
ATCAACG50400.033.9530453
CAACGCA51550.033.4710965
AACGCAG53850.032.1160436
TATCAAC54000.031.9506872
ACGCAGA61650.027.8232237
CGCAGAG62300.027.6075828
GTACATG45950.024.7768461
GTGGTAT11950.024.408441
TACATGG45450.024.2041512
GCAGAGT71550.023.6443929
ACATGGG45600.022.9904733
GAGTACT49800.021.23067912-13
GTGCGCG1857.192608E-620.3213989
AGAGTAC66450.019.9075910-11
CAGAGTA72050.019.63214910-11
CTAGACG1200.005057768419.5882954
CGGGTCG600.00506955819.58106858-59
Produced by FastQC (version 0.11.5)