##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033159_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1762144 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.70392941780013 34.0 31.0 34.0 31.0 34.0 2 32.785669616104016 34.0 31.0 34.0 31.0 34.0 3 32.89128754517225 34.0 31.0 34.0 31.0 34.0 4 36.3354720159079 37.0 37.0 37.0 35.0 37.0 5 36.27244595220368 37.0 37.0 37.0 35.0 37.0 6 36.35896328563386 37.0 37.0 37.0 35.0 37.0 7 36.32548645286651 37.0 37.0 37.0 35.0 37.0 8 36.31778049921005 37.0 37.0 37.0 35.0 37.0 9 38.12695159986925 39.0 39.0 39.0 37.0 39.0 10-11 38.10644589772459 39.0 39.0 39.0 36.0 39.0 12-13 38.070502751194 39.0 39.0 39.0 35.0 39.0 14-15 39.58074481994662 41.0 40.0 41.0 37.0 41.0 16-17 39.592974240470696 41.0 40.0 41.0 37.0 41.0 18-19 39.610172324168744 41.0 40.0 41.0 37.0 41.0 20-21 39.60654237111156 41.0 40.0 41.0 37.0 41.0 22-23 39.5422281039461 41.0 39.5 41.0 37.0 41.0 24-25 39.500055614070135 41.0 39.0 41.0 36.5 41.0 26-27 39.398207808215446 41.0 39.0 41.0 36.0 41.0 28-29 39.310758371620025 41.0 39.0 41.0 36.0 41.0 30-31 39.22139450578386 41.0 39.0 41.0 36.0 41.0 32-33 39.27047363893075 41.0 39.0 41.0 35.5 41.0 34-35 39.22224290409864 41.0 39.0 41.0 35.0 41.0 36-37 39.1449529663864 41.0 39.0 41.0 35.0 41.0 38-39 39.05739769281058 41.0 39.0 41.0 35.0 41.0 40-41 38.94916590244611 41.0 39.0 41.0 35.0 41.0 42-43 38.84885202344417 41.0 39.0 41.0 35.0 41.0 44-45 38.72000160032324 41.0 39.0 41.0 35.0 41.0 46-47 38.526925722301925 41.0 38.5 41.0 35.0 41.0 48-49 38.360144517133676 41.0 38.0 41.0 34.5 41.0 50-51 38.237978564748396 40.0 38.0 41.0 34.0 41.0 52-53 38.112615938311514 40.0 38.0 41.0 34.0 41.0 54-55 37.98760969591588 40.0 38.0 41.0 34.0 41.0 56-57 37.839820979443225 40.0 37.5 41.0 34.0 41.0 58-59 37.66192150017252 40.0 37.0 41.0 33.0 41.0 60-61 37.475396165126114 40.0 36.5 41.0 33.0 41.0 62-63 37.25363506047179 39.5 36.0 41.0 33.0 41.0 64-65 37.01134101412825 39.0 35.5 41.0 33.0 41.0 66-67 36.742859550638315 39.0 35.0 41.0 32.0 41.0 68-69 36.43345067145477 38.5 35.0 41.0 32.0 41.0 70-71 36.106988134908384 37.5 35.0 40.0 32.0 41.0 72-73 35.75550040178328 37.0 35.0 39.5 31.0 41.0 74-75 35.41954828890261 36.5 35.0 39.0 31.0 41.0 76-77 35.0738239894129 36.0 35.0 39.0 31.0 41.0 78-79 34.72876365382171 36.0 35.0 38.5 31.0 39.5 80-81 34.37720952430675 35.0 35.0 37.0 31.0 39.0 82-83 34.0403525478054 35.0 34.0 37.0 30.0 39.0 84-85 33.75414722065847 35.0 34.0 36.0 30.0 38.0 86-87 33.50453339795159 35.0 34.0 36.0 30.0 37.0 88-89 33.27077270643035 35.0 34.0 36.0 30.0 37.0 90-91 33.056772602012096 35.0 34.0 35.0 30.0 36.5 92-93 32.883319127154195 35.0 34.0 35.0 29.0 36.0 94-95 32.72516604772368 35.0 34.0 35.0 29.0 36.0 96-97 32.59147436304865 35.0 34.0 35.0 29.0 36.0 98-99 32.46966763215719 35.0 34.0 35.0 29.0 36.0 100 32.36400600631958 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07670501134744256 1101 2 0.12056540621119183 1101 3 0.10422123059140631 1101 4 0.09180966899028675 1101 5 0.044708492888346996 1101 6 0.0845218815172899 1101 7 0.10315166953990484 1101 8 0.11836106764239673 1101 9 0.17494396607011709 1101 10-11 0.14816534758225686 1101 12-13 0.11295649445280986 1101 14-15 0.16092950636815573 1101 16-17 0.14665272869428492 1101 18-19 0.17843130897198023 1101 20-21 0.1518905744100678 1101 22-23 0.23727378273855493 1101 24-25 0.18145821425309094 1101 26-27 0.21288956675857662 1101 28-29 0.18769894497888373 1101 30-31 0.2684342452834798 1101 32-33 0.22749145357496303 1101 34-35 0.19936174635024173 1101 36-37 0.23999773944821357 1101 38-39 0.20069520489401071 1101 40-41 0.2448826922416032 1101 42-43 0.24209212497927268 1101 44-45 0.1882928045872987 1101 46-47 0.20217595952582457 1101 48-49 0.24526437957226932 1101 50-51 0.25920681819751934 1101 52-53 0.23753159041545757 1101 54-55 0.21171236767993662 1101 56-57 0.19342311200905016 1101 58-59 0.18412966725622226 1101 60-61 0.06884355877274118 1101 62-63 0.12455423638564156 1101 64-65 0.1322742341987464 1101 66-67 0.15263669709614192 1101 68-69 0.1989904889134948 1101 70-71 0.18910602724953662 1101 72-73 0.20297966537576428 1101 74-75 0.24003421146311155 1101 76-77 0.2010467090727346 1101 78-79 0.05942325278683569 1101 80-81 0.09280357045666676 1101 82-83 0.1312562682755214 1101 84-85 0.21619512146298092 1101 86-87 0.1318089336953392 1101 88-89 0.09162574377064203 1101 90-91 0.14017936698643751 1101 92-93 0.06877512871798075 1101 94-95 0.19664946072936118 1101 96-97 0.25023694649751604 1101 98-99 0.2694035689008558 1101 100 0.23042258045995112 1102 1 0.06580213320012973 1102 2 0.06209204200366969 1102 3 0.06873657569261837 1102 4 0.04232225463303507 1102 5 0.014301812204649877 1102 6 0.061368864068811035 1102 7 0.10793070788798076 1102 8 0.08804719974023811 1102 9 0.20385170393402774 1102 10-11 0.13092471749169476 1102 12-13 0.107722001156489 1102 14-15 0.1758738400166564 1102 16-17 0.10579731297009687 1102 18-19 0.08998376180390721 1102 20-21 0.17895861864141693 1102 22-23 0.1884452407420909 1102 24-25 0.18090609298909044 1102 26-27 0.22849843008405202 1102 28-29 0.21442156496236464 1102 30-31 0.18643538264999648 1102 32-33 0.17381803518838979 1102 34-35 0.24773950683358237 1102 36-37 0.18813933567719232 1102 38-39 0.14905866630570586 1102 40-41 0.2178073508922651 1102 42-43 0.3092537330213858 1102 44-45 0.33661919008043384 1102 46-47 0.3684444003268439 1102 48-49 0.44815082428193165 1102 50-51 0.4958820347907391 1102 52-53 0.5393032486715796 1102 54-55 0.4855782069682135 1102 56-57 0.4213735171876678 1102 58-59 0.5239719478400815 1102 60-61 0.5518220062018457 1102 62-63 0.5680719195162496 1102 64-65 0.484670263385901 1102 66-67 0.4938307482887865 1102 68-69 0.4110302252464848 1102 70-71 0.3914384649231337 1102 72-73 0.4108231489182259 1102 74-75 0.3178649439068195 1102 76-77 0.37329837666916177 1102 78-79 0.33780666402714843 1102 80-81 0.3272489845705735 1102 82-83 0.33646040884064377 1102 84-85 0.36928586907814065 1102 86-87 0.3601406747811282 1102 88-89 0.30211652332912564 1102 90-91 0.1799012697206166 1102 92-93 0.31009360807075126 1102 94-95 0.3056788161331099 1102 96-97 0.3041435446991798 1102 98-99 0.2940761086816295 1102 100 0.3081578764392532 1103 1 0.08000271746691823 1103 2 0.08361568483043413 1103 3 0.10811875316962016 1103 4 0.07195535888518378 1103 5 0.010935422592297073 1103 6 0.06932610309488041 1103 7 0.09663364261495389 1103 8 0.0872903824195177 1103 9 0.12440910832101792 1103 10-11 0.1135436076392935 1103 12-13 0.07849482330250623 1103 14-15 0.14333992082591607 1103 16-17 0.18123400992536176 1103 18-19 0.14314055532892667 1103 20-21 0.11599342174007887 1103 22-23 0.11041789988129835 1103 24-25 0.14616248354627714 1103 26-27 0.12856927632925164 1103 28-29 0.15468206727400968 1103 30-31 0.13512760613729569 1103 32-33 0.10730949212376828 1103 34-35 0.08098758289833086 1103 36-37 0.022251039241488968 1103 38-39 0.0017250396619132857 1103 40-41 0.11921407991761868 1103 42-43 0.03576296959662528 1103 44-45 0.03205811708588868 1103 46-47 0.12142053115476159 1103 48-49 0.13823230223576388 1103 50-51 0.12285309217492113 1103 52-53 0.10574745112960215 1103 54-55 0.05103749709965655 1103 56-57 0.12443811833794172 1103 58-59 0.1077304540818389 1103 60-61 0.11972520548084731 1103 62-63 -0.01462389304755618 1103 64-65 -0.10203677641492703 1103 66-67 -0.012865764784365297 1103 68-69 -0.06357145947531961 1103 70-71 -0.08523261730655207 1103 72-73 -0.16198488829720503 1103 74-75 -0.19599317972704 1103 76-77 -0.16495676652328228 1103 78-79 -0.17738463833170925 1103 80-81 -0.13252175290797652 1103 82-83 -0.133345634054578 1103 84-85 -0.1634215868290596 1103 86-87 -0.1377628076283557 1103 88-89 -0.1788900341636932 1103 90-91 -0.22657532413698078 1103 92-93 -0.09959508519300186 1103 94-95 -0.0786218213558385 1103 96-97 -0.08176242671354572 1103 98-99 -0.08068470173324727 1103 100 -0.08280523484025082 1104 1 -0.05122421514957409 1104 2 0.0022045188259696147 1104 3 -0.004687286090707232 1104 4 0.030338849225998388 1104 5 0.0043886721855841415 1104 6 0.042190576160493265 1104 7 0.09049283536275254 1104 8 0.06180094274945702 1104 9 -2.1024355160648156E-4 1104 10-11 0.06186594362205966 1104 12-13 0.11327036547199043 1104 14-15 0.04844566473813927 1104 16-17 0.06721610196318295 1104 18-19 0.043688150635396994 1104 20-21 0.07106043598538037 1104 22-23 0.07313058444329812 1104 24-25 0.07066851981093691 1104 26-27 0.0483048691682626 1104 28-29 0.03584587695269903 1104 30-31 0.05361159186325182 1104 32-33 0.08791638325335782 1104 34-35 0.14561385536811144 1104 36-37 0.05142759211160808 1104 38-39 -0.006873435483541357 1104 40-41 -0.0028704198399722713 1104 42-43 -0.009181479161306072 1104 44-45 0.05807225662396576 1104 46-47 0.07123580867865797 1104 48-49 0.06140450344417303 1104 50-51 0.04264416594958931 1104 52-53 0.13498620989727073 1104 54-55 -0.02925044742796956 1104 56-57 0.067030939624523 1104 58-59 0.1364827229922767 1104 60-61 0.10829597989688722 1104 62-63 0.09367244790067986 1104 64-65 0.10071837933825378 1104 66-67 0.11227874547640226 1104 68-69 0.1866012497733962 1104 70-71 0.14347844592825965 1104 72-73 0.15420218669284225 1104 74-75 0.16488447670933226 1104 76-77 0.06982372766359646 1104 78-79 0.12746094125913032 1104 80-81 0.13496375938433403 1104 82-83 0.11744531316853113 1104 84-85 0.06871844277299743 1104 86-87 0.09562852340666694 1104 88-89 0.06769292099242108 1104 90-91 0.035730591934267864 1104 92-93 -0.00642471195150307 1104 94-95 0.005362406774423789 1104 96-97 -0.03211004610760426 1104 98-99 -0.00797899890442011 1104 100 -0.060912121384639306 1105 1 0.011536018420109428 1105 2 0.041296457096841266 1105 3 0.0564400451373146 1105 4 0.0435903537245963 1105 5 0.017044037734059714 1105 6 -0.008736552481586557 1105 7 -0.04785728082624985 1105 8 -0.030111111114571543 1105 9 0.046888010634177135 1105 10-11 -0.031155864159622126 1105 12-13 0.061340734297587574 1105 14-15 0.06878767748913361 1105 16-17 0.07984763387047167 1105 18-19 0.08810944003538168 1105 20-21 0.11413348609561069 1105 22-23 0.11726721079138258 1105 24-25 0.04069712018036853 1105 26-27 -2.369588720156912E-4 1105 28-29 0.10933661162468411 1105 30-31 0.1070402101079111 1105 32-33 0.0471026133671657 1105 34-35 0.14432966898903743 1105 36-37 0.1453602918673198 1105 38-39 0.09060898720608179 1105 40-41 0.17628408173900567 1105 42-43 0.22126718913399657 1105 44-45 0.2718828089685914 1105 46-47 0.31549041155060564 1105 48-49 0.29957542044352437 1105 50-51 0.3090504997397403 1105 52-53 0.2860810677891834 1105 54-55 0.3326318143845839 1105 56-57 0.25169875825051946 1105 58-59 0.2377603551200238 1105 60-61 0.10996016389260177 1105 62-63 0.16979113943377655 1105 64-65 0.1382830280899654 1105 66-67 0.17239165735151118 1105 68-69 0.14603937168246972 1105 70-71 0.17360640278936756 1105 72-73 0.19954613592719284 1105 74-75 0.09961485864342734 1105 76-77 0.04373244481786287 1105 78-79 0.08589536895382821 1105 80-81 0.0951043778673224 1105 82-83 0.053335350706518625 1105 84-85 0.11214289013044976 1105 86-87 0.1644381819897589 1105 88-89 0.08752578758362262 1105 90-91 0.11592528299069471 1105 92-93 0.1950791908072418 1105 94-95 0.1457926039978048 1105 96-97 0.19985905953500804 1105 98-99 0.13277622960061564 1105 100 0.050695159792745415 1106 1 0.03812293095181474 1106 2 0.08877501040718272 1106 3 0.05887399503328794 1106 4 0.03606334942100631 1106 5 0.0032310870685705595 1106 6 0.05250236791628282 1106 7 0.05821533866244977 1106 8 0.04501186890652065 1106 9 0.10184996235418708 1106 10-11 0.07786499826805482 1106 12-13 0.08831268972267026 1106 14-15 0.09291769715454024 1106 16-17 0.127043033876177 1106 18-19 0.12074253776388844 1106 20-21 0.11577497724617558 1106 22-23 0.14386381352174027 1106 24-25 0.11410436502887933 1106 26-27 0.07077360059445681 1106 28-29 -0.012415176786973348 1106 30-31 0.06544695619620455 1106 32-33 0.05067936544058682 1106 34-35 0.17619823854471406 1106 36-37 0.21147302592836326 1106 38-39 0.1436278403425746 1106 40-41 0.1906127504767099 1106 42-43 0.1560028462149674 1106 44-45 0.12880153955257612 1106 46-47 0.1462275791625487 1106 48-49 0.09126315379205607 1106 50-51 0.049226989607774385 1106 52-53 0.06283100366287186 1106 54-55 0.01652955428824754 1106 56-57 0.1229353986016477 1106 58-59 0.039999574774029156 1106 60-61 0.07277345656510192 1106 62-63 0.08761682986042985 1106 64-65 0.10417987022523079 1106 66-67 0.10835440708788724 1106 68-69 0.16251983453196317 1106 70-71 0.14001882049393544 1106 72-73 0.19924210594457747 1106 74-75 0.1437445933029906 1106 76-77 0.0531721233312723 1106 78-79 0.09811371192985519 1106 80-81 0.03988278582468752 1106 82-83 0.07438923106106188 1106 84-85 0.05181335212491689 1106 86-87 0.03834183576466188 1106 88-89 0.09369345652083183 1106 90-91 0.07020272192819732 1106 92-93 0.05134333422217452 1106 94-95 0.04612577582783217 1106 96-97 0.07720002627483069 1106 98-99 0.08261505879661257 1106 100 0.14811457439073905 1107 1 -0.0911842198447772 1107 2 -0.04238467836643878 1107 3 -0.04153356332165714 1107 4 -0.0035809632874048702 1107 5 -0.029744088922534218 1107 6 -0.14942427157982507 1107 7 -0.06405216782142986 1107 8 -0.07466208440952471 1107 9 -0.1330518531330327 1107 10-11 -0.04366149302502009 1107 12-13 -0.06229864780974026 1107 14-15 -0.03620419984434875 1107 16-17 -0.027458633408002697 1107 18-19 -0.053951793469906306 1107 20-21 -0.0687378052658616 1107 22-23 -0.05509876521112034 1107 24-25 -0.08078263015451626 1107 26-27 -5.491115211455622E-4 1107 28-29 -0.04576086037349114 1107 30-31 -0.04088872368523511 1107 32-33 -0.024163168831371706 1107 34-35 0.04985514851136941 1107 36-37 -0.0767818832972722 1107 38-39 -0.015134664288773081 1107 40-41 0.04333598130294547 1107 42-43 -3.5135904624894465E-4 1107 44-45 0.04414055400236805 1107 46-47 0.030073448002859493 1107 48-49 0.04309716638930894 1107 50-51 0.04774495922615074 1107 52-53 0.08320892141018277 1107 54-55 0.0774780537324915 1107 56-57 0.11811879986137086 1107 58-59 0.040925843762543934 1107 60-61 0.037945742596960486 1107 62-63 0.10169611848584736 1107 64-65 0.00525434225190935 1107 66-67 0.04018100953323511 1107 68-69 0.020800679289287416 1107 70-71 0.04239006910453469 1107 72-73 -0.016949075974650896 1107 74-75 -0.025577377797411316 1107 76-77 0.04150113407599321 1107 78-79 0.06458277454564154 1107 80-81 -0.006060156005617046 1107 82-83 0.07068415510700987 1107 84-85 0.0505165755409962 1107 86-87 0.03519252120371874 1107 88-89 0.1350050534085696 1107 90-91 0.08557878713752842 1107 92-93 0.06412584626367135 1107 94-95 0.07446960687636306 1107 96-97 0.03292436862384562 1107 98-99 0.01258912326493089 1107 100 0.05659919832553584 1108 1 0.02667343062897487 1108 2 -0.007463501818300244 1108 3 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2214 20-21 -0.19228017863094493 2214 22-23 -0.17376708211407532 2214 24-25 -0.15901081333124978 2214 26-27 -0.18551473664979312 2214 28-29 -0.13120478630934684 2214 30-31 -0.058164682136855106 2214 32-33 -0.08671745375045248 2214 34-35 -0.08759616080489252 2214 36-37 -0.1041013252476759 2214 38-39 -0.11387727465748299 2214 40-41 -0.07285073027924938 2214 42-43 -0.07866149840205594 2214 44-45 -0.10947645378637816 2214 46-47 -0.09028406278214618 2214 48-49 -0.16632739010652386 2214 50-51 -0.14532302550802 2214 52-53 -0.1291580387075939 2214 54-55 -0.12819299815802765 2214 56-57 -0.0763836705681058 2214 58-59 -0.14250142978900016 2214 60-61 -0.1448460864351233 2214 62-63 -0.18594531906341416 2214 64-65 -0.14693051603462237 2214 66-67 -0.23439744652348082 2214 68-69 -0.11371365647546128 2214 70-71 0.007905207256889923 2214 72-73 -0.05889744332927194 2214 74-75 -0.005450948078816964 2214 76-77 0.06590904803741182 2214 78-79 -0.10344892342727974 2214 80-81 0.012637281228450092 2214 82-83 0.001970844388026194 2214 84-85 -0.03922948153334005 2214 86-87 0.032415608448779665 2214 88-89 -0.1525971739810572 2214 90-91 0.027383649096300644 2214 92-93 0.018065400235165896 2214 94-95 -0.036962888634988644 2214 96-97 -0.24342328014304115 2214 98-99 -0.2773839612651017 2214 100 -0.3969156104867402 2215 1 -0.057619445038902484 2215 2 -0.08899061456999391 2215 3 -0.0702050574604769 2215 4 -0.09811590322475894 2215 5 -0.049750160183663183 2215 6 -0.06858594093807113 2215 7 -0.0632186474491121 2215 8 -0.019976672622703973 2215 9 -0.15011492073143273 2215 10-11 -0.06975537896807538 2215 12-13 -0.10692369509693123 2215 14-15 -0.1961370167391081 2215 16-17 -0.12235543900933976 2215 18-19 -0.1321066218265372 2215 20-21 -0.12788561832165612 2215 22-23 -0.12122414673414283 2215 24-25 -0.15537990397812962 2215 26-27 -0.09958978410197261 2215 28-29 -0.104228030041142 2215 30-31 -0.12166114438516473 2215 32-33 -0.15085023207910808 2215 34-35 -0.20738593800580674 2215 36-37 -0.18539605100137635 2215 38-39 -0.2249547952663704 2215 40-41 -0.2086598179880781 2215 42-43 -0.12319556230642092 2215 44-45 -0.11610945829108488 2215 46-47 -0.1446783946760135 2215 48-49 -0.09416737749441495 2215 50-51 -0.08240532761293906 2215 52-53 -0.1386460119641768 2215 54-55 -0.17967436169951867 2215 56-57 -0.30462945361646376 2215 58-59 -0.2983856572119308 2215 60-61 -0.24138978051253446 2215 62-63 -0.2532984188397549 2215 64-65 -0.11868123957403753 2215 66-67 -0.20363179357337913 2215 68-69 -0.1733404928682134 2215 70-71 -0.18020563602595274 2215 72-73 -0.10950142295283172 2215 74-75 -0.15481301965435534 2215 76-77 -0.22660365141179994 2215 78-79 -0.09909704992821844 2215 80-81 -0.03201479730704193 2215 82-83 -0.04673717532198651 2215 84-85 -0.018001403814771777 2215 86-87 -0.08152942136614882 2215 88-89 -0.13932198356770442 2215 90-91 -0.07437922524744778 2215 92-93 -0.09774300670615332 2215 94-95 0.023608736616836268 2215 96-97 0.007828078032936503 2215 98-99 -0.06977304766614623 2215 100 -0.051190516948388165 2216 1 -0.0756607318621576 2216 2 -0.0869476153591009 2216 3 -0.14979921655822892 2216 4 -0.027333999522667796 2216 5 -0.056963865658019586 2216 6 -0.07148408573964815 2216 7 -0.06759563436804683 2216 8 -0.08299413109395459 2216 9 -0.10232655909730681 2216 10-11 -0.10260081040673441 2216 12-13 -0.10844499050728018 2216 14-15 -0.18306790152023922 2216 16-17 -0.11492999118475211 2216 18-19 -0.08351112351083145 2216 20-21 -0.1204335454688703 2216 22-23 -0.12863701580524634 2216 24-25 -0.20859746525972156 2216 26-27 -0.2016106027775706 2216 28-29 -0.2447876336259185 2216 30-31 -0.2338670845757136 2216 32-33 -0.1880518954934729 2216 34-35 -0.21342436861343117 2216 36-37 -0.1957855772603665 2216 38-39 -0.19244081398188229 2216 40-41 -0.19796116886234216 2216 42-43 -0.15619047126559593 2216 44-45 -0.10455440569931795 2216 46-47 -0.17682832587023256 2216 48-49 -0.24294740612365473 2216 50-51 -0.027511647430259245 2216 52-53 0.05211979931929278 2216 54-55 0.030602458928299825 2216 56-57 0.10112392147274818 2216 58-59 -0.004752162674876104 2216 60-61 -0.005874110320746695 2216 62-63 0.01625179428626211 2216 64-65 0.07981473983705456 2216 66-67 0.06308799037394408 2216 68-69 0.062170145822186385 2216 70-71 0.048988686427875905 2216 72-73 0.10817715551385731 2216 74-75 0.06735281947009497 2216 76-77 0.023553268537860106 2216 78-79 0.05375482602515547 2216 80-81 0.08583973695622404 2216 82-83 0.05819164151208156 2216 84-85 0.0952157633325399 2216 86-87 0.060611541176847084 2216 88-89 0.06822702954523407 2216 90-91 -0.011539127200258292 2216 92-93 0.07337038907929383 2216 94-95 0.09331211703104714 2216 96-97 0.18747196513610476 2216 98-99 0.21034164121516596 2216 100 0.1868502331813815 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 5.0 4 9.0 5 16.0 6 24.0 7 92.0 8 174.0 9 358.0 10 685.0 11 1115.0 12 1621.0 13 2446.0 14 3019.0 15 4079.0 16 4958.0 17 7081.0 18 8294.0 19 4645.0 20 3149.0 21 3015.0 22 3239.0 23 3497.0 24 4011.0 25 4906.0 26 6216.0 27 7841.0 28 10336.0 29 13326.0 30 17166.0 31 21818.0 32 28276.0 33 36950.0 34 51549.0 35 78206.0 36 142628.0 37 328676.0 38 625226.0 39 332044.0 40 1438.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.96833420143671 19.93396355556212 13.452894930711718 23.644807312289448 2 15.502916940957483 19.938256191405806 40.72441150432434 23.834415363312374 3 18.38538734632357 25.12723137269145 30.14520947209763 26.342171808887354 4 11.956895543935833 16.582866949126572 39.223942733202506 32.23629477373508 5 12.172390105991019 38.07376793246549 36.1924415403044 13.561400421239078 6 30.90011627913575 38.743708640464796 17.344488715135412 13.011686365264033 7 27.077698388826775 32.62010022303314 22.43775786433002 17.864443523810063 8 24.969610848614924 36.98459034515947 19.978242389092365 18.06755641713324 9 24.699003032669296 16.374938711024754 21.313695135017344 37.61236312128861 10-11 23.754240587286578 26.864922043562988 29.052037922115066 20.32879944703536 12-13 24.57372950224272 24.055497167087367 29.49756092578132 21.87321240488859 14-15 21.486798031373738 26.187240816222296 26.77496286480804 25.55099828759592 16-17 20.745807380100604 29.865805518731726 27.273423738355095 22.114963362812574 18-19 20.576667651264103 28.410317786624965 30.062398507724836 20.9506160543861 20-21 21.697347095356566 27.699495614433328 29.759855040223727 20.84330224998638 22-23 21.128830707173428 27.472785961630784 29.960752311675222 21.437631019520563 24-25 21.068171880635013 27.62786075919556 29.932925363825774 21.371041996343653 26-27 20.92269076191265 27.51155143980142 30.05405994377221 21.511697854513717 28-29 20.981874543486736 27.625529441877596 30.039977366590293 21.35261864804537 30-31 21.177471070176395 28.12721401711265 29.68020509746217 21.015109815248785 32-33 20.820012805870718 27.76532447215518 30.118488362324403 21.2961743596497 34-35 20.847935082941532 27.557762473160548 30.221995790836058 21.372306653061862 36-37 21.222090089374156 27.979048586730393 29.578559285233823 21.22030203866163 38-39 21.240555303589765 28.071360496065456 29.577942479521234 21.110141720823545 40-41 21.000803533389778 27.859107189537664 29.793718134337954 21.346371142734608 42-43 21.01864171266265 27.80641726285485 29.664798824803544 21.510142199678956 44-45 21.187912617474122 28.013970033562817 29.46594648226132 21.332170866701745 46-47 21.248405814046546 28.246931812828524 29.18774984093614 21.31691253218879 48-49 21.274913626141306 28.1609852650099 29.007768326510924 21.556332782337876 50-51 21.31991535824478 28.14772523154605 28.952202152706462 21.580157257502705 52-53 21.38495559024356 28.110818239937256 28.92160381917379 21.582622350645398 54-55 21.421757241441934 28.560451284845183 28.65809157359484 21.35969990011804 56-57 21.208527880756087 28.261267434783164 28.811888071210383 21.718316613250362 58-59 21.084857838200882 27.882002996942052 29.18023334834358 21.852905816513484 60-61 21.334598466744563 28.26994330166906 28.86280433996753 21.53265389161885 62-63 21.17358636188228 28.257581776496494 28.87992872222096 21.688903139400267 64-65 21.210308632446814 27.98008735051893 29.023957832036395 21.785646184997866 66-67 21.078430120725677 28.16392382992114 28.8062126890923 21.95143336026089 68-69 21.5189431632746 28.17422585722421 28.781418360146105 21.52541261935508 70-71 21.470071316734945 28.037827889854317 28.765409539202075 21.726691254208664 72-73 21.25658681199009 28.207913282639247 28.943695401922152 21.591804503448508 74-75 21.316323290689056 28.207535686103597 28.741064464111872 21.735076559095486 76-77 21.36776873574177 28.22899996595049 28.597217020214057 21.806014278093684 78-79 21.483636264583446 28.35075576989048 28.58047599415144 21.585131971374636 80-81 21.40989885272597 28.105532299340542 28.775969584597306 21.70859926333618 82-83 21.321935423826538 28.208832916415076 28.806346388700987 21.662885271057398 84-85 21.288439422645027 28.103790874672523 28.68921558139977 21.91855412128268 86-87 21.415407664249628 28.24541715553651 28.60571718802537 21.733457992188487 88-89 21.199629542194053 28.12168018050738 28.63684806690032 22.04184221039824 90-91 21.11036328472588 28.03652822924801 28.769839468284093 22.083269017742026 92-93 21.269771369422703 28.27510124030726 28.57672244720068 21.878404943069352 94-95 21.079264804692464 27.959292770624877 28.734825303720925 22.226617120961738 96-97 21.037837997348685 27.99748488205277 28.76864773820982 22.196029382388726 98-99 21.027254299308115 28.091716681497086 28.65974630904171 22.221282710153087 100 21.151506346813882 27.941246572357308 28.564294404997547 22.34295267583126 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 42.0 1 32.5 2 88.5 3 176.0 4 128.0 5 80.0 6 202.5 7 477.0 8 795.5 9 906.5 10 717.5 11 548.0 12 574.5 13 674.5 14 783.5 15 901.5 16 1058.5 17 1260.5 18 1490.0 19 1802.0 20 2240.0 21 2936.5 22 3911.0 23 5372.0 24 7328.5 25 9668.5 26 13078.0 27 17326.5 28 22263.0 29 27498.5 30 32852.0 31 38471.0 32 43409.5 33 48158.0 34 52805.5 35 55915.5 36 58485.5 37 61853.5 38 63937.5 39 63914.5 40 63974.0 41 64159.0 42 63687.0 43 62755.5 44 60915.5 45 59618.5 46 57916.5 47 55368.0 48 53651.0 49 52305.5 50 50824.0 51 48901.0 52 45620.0 53 42798.5 54 41574.0 55 39703.0 56 36831.5 57 34230.5 58 31162.5 59 27518.5 60 24076.5 61 20282.5 62 17187.5 63 14325.0 64 11359.5 65 9028.0 66 7076.5 67 5379.5 68 4170.0 69 3302.0 70 2419.0 71 1748.0 72 1343.5 73 956.5 74 686.0 75 464.0 76 254.0 77 169.5 78 122.0 79 59.0 80 38.5 81 20.0 82 9.0 83 3.0 84 0.0 85 1.0 86 1.5 87 1.5 88 1.5 89 0.5 90 0.5 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09085523090053935 2 0.0013619772277407521 3 0.0 4 3.4049430693518804E-4 5 0.038248860479052786 6 2.8374525577932336E-4 7 0.004199429785533986 8 1.1349810231172935E-4 9 0.0 10-11 3.4049430693518804E-4 12-13 0.0 14-15 8.512357673379701E-5 16-17 0.0 18-19 1.4187262788966168E-4 20-21 0.0 22-23 1.1349810231172935E-4 24-25 1.4187262788966168E-4 26-27 0.0014754753300524816 28-29 0.008342110519912108 30-31 0.03631939273975339 32-33 0.025650571122450835 34-35 0.02902713966622478 36-37 0.02548032396898324 38-39 0.025026331559736322 40-41 0.031183603610147638 42-43 0.02247262425772241 44-45 0.021167396081137525 46-47 0.015492490965551055 48-49 0.01029995278478944 50-51 0.007349002124684476 52-53 0.0049655419761381586 54-55 0.003631939273975339 56-57 0.002355085622968384 58-59 8.228612417600378E-4 60-61 6.242395627145113E-4 62-63 0.0013619772277407521 64-65 0.0027239544554815043 66-67 4.823669348248497E-4 68-69 9.931083952276318E-4 70-71 2.8374525577932336E-4 72-73 3.1211978135725567E-4 74-75 4.823669348248497E-4 76-77 2.269962046234587E-4 78-79 2.8374525577932338E-5 80-81 5.958650371365791E-4 82-83 4.539924092469174E-4 84-85 2.5537073020139104E-4 86-87 8.512357673379701E-5 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1762144.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.46602037126279 #Duplication Level Percentage of deduplicated Percentage of total 1 81.39674239293264 58.98497992400685 2 12.494189047986055 18.10808316147532 3 3.172347769109571 6.896622541830727 4 1.2084126363180907 3.5027541888127254 5 0.5509331151526088 1.9961965172926106 6 0.3024600338062672 1.3150844982778676 7 0.20031206984296146 1.0161072973694902 8 0.14068069977328335 0.8155656364491405 9 0.09693661657038385 0.6322149747999652 >10 0.41827858359440573 5.085009803841331 >50 0.014238345086093111 0.6960926480301153 >100 0.004155204936817887 0.5110332996274428 >500 7.837122271242085E-5 0.049693442938991805 >1k 2.3511366813726257E-4 0.39056206524736753 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3050 0.17308460602538725 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2473 0.14034040350845334 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 5.6749051155864675E-5 0.0 4 0.0 0.0 0.0 1.7024715346759402E-4 0.0 5 0.0 0.0 0.0 1.7024715346759402E-4 0.0 6 0.0 0.0 0.0 1.7024715346759402E-4 0.0 7 0.0 0.0 0.0 1.7024715346759402E-4 0.0 8 0.0 0.0 0.0 1.7024715346759402E-4 0.0 9 0.0 0.0 0.0 2.269962046234587E-4 0.0 10-11 0.0 0.0 0.0 2.8374525577932336E-4 0.0 12-13 0.0 0.0 0.0 2.8374525577932336E-4 0.0 14-15 0.0 0.0 0.0 3.121197813572557E-4 0.0 16-17 0.0 0.0 0.0 3.6886883251312035E-4 0.0 18-19 0.0 0.0 0.0 3.972433580910527E-4 0.0 20-21 0.0 0.0 0.0 3.972433580910527E-4 0.0 22-23 0.0 0.0 0.0 4.256178836689851E-4 0.0 24-25 0.0 0.0 0.0 5.674905115586467E-4 0.0 26-27 0.0 0.0 0.0 5.958650371365791E-4 0.0 28-29 0.0 0.0 0.0 9.647338696496994E-4 0.0 30-31 0.0 0.0 0.0 0.0033481940181960155 0.0 32-33 0.0 0.0 0.0 0.008398859571067972 0.0 34-35 0.0 0.0 0.0 0.01543574191439519 0.0 36-37 0.0 0.0 0.0 0.02987837543356275 0.0 38-39 0.0 0.0 0.0 0.05445071458405215 0.0 40-41 0.0 0.0 0.0 0.08424396644088111 0.0 42-43 0.0 0.0 0.0 0.11491682859062596 0.0 44-45 0.0 0.0 0.0 0.15027148746072966 0.0 46-47 0.0 0.0 0.0 0.1945357473623041 0.0 48-49 0.0 0.0 0.0 0.24535452267238095 0.0 50-51 0.0 0.0 0.0 0.3017347049957325 0.0 52-53 0.0 0.0 0.0 0.3590512466631558 0.0 54-55 0.0 0.0 0.0 0.4187796230047034 0.0 56-57 0.0 0.0 0.0 0.488552581400839 2.8374525577932338E-5 58-59 0.0 0.0 0.0 0.5697037245537255 5.6749051155864675E-5 60-61 0.0 0.0 0.0 0.6578066264732054 5.6749051155864675E-5 62-63 0.0 5.6749051155864675E-5 0.0 0.7491442243085695 5.6749051155864675E-5 64-65 0.0 5.6749051155864675E-5 0.0 0.8460716036827864 5.6749051155864675E-5 66-67 0.0 5.6749051155864675E-5 0.0 0.9542920442370204 5.6749051155864675E-5 68-69 0.0 5.6749051155864675E-5 0.0 1.0763592532732853 5.6749051155864675E-5 70-71 0.0 5.6749051155864675E-5 0.0 1.2125569760473605 5.6749051155864675E-5 72-73 0.0 5.6749051155864675E-5 0.0 1.3575224272250168 5.6749051155864675E-5 74-75 0.0 5.6749051155864675E-5 0.0 1.5069994279695644 5.6749051155864675E-5 76-77 0.0 5.6749051155864675E-5 0.0 1.661044727332159 5.6749051155864675E-5 78-79 0.0 5.6749051155864675E-5 0.0 1.837534276426898 5.6749051155864675E-5 80-81 0.0 5.6749051155864675E-5 0.0 2.0371206893420744 5.6749051155864675E-5 82-83 0.0 5.6749051155864675E-5 0.0 2.245588328763143 5.6749051155864675E-5 84-85 0.0 5.6749051155864675E-5 0.0 2.4476433254035994 5.6749051155864675E-5 86-87 0.0 5.6749051155864675E-5 0.0 2.670837343599615 5.6749051155864675E-5 88 0.0 5.6749051155864675E-5 0.0 2.8516398205822 5.6749051155864675E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2370 0.0 52.603394 1 GTATCAA 4270 0.0 41.095776 1 TCAACGC 5035 0.0 34.08013 4 ATCAACG 5040 0.0 33.953045 3 CAACGCA 5155 0.0 33.471096 5 AACGCAG 5385 0.0 32.116043 6 TATCAAC 5400 0.0 31.950687 2 ACGCAGA 6165 0.0 27.823223 7 CGCAGAG 6230 0.0 27.607582 8 GTACATG 4595 0.0 24.776846 1 GTGGTAT 1195 0.0 24.40844 1 TACATGG 4545 0.0 24.204151 2 GCAGAGT 7155 0.0 23.644392 9 ACATGGG 4560 0.0 22.990473 3 GAGTACT 4980 0.0 21.230679 12-13 GTGCGCG 185 7.192608E-6 20.321398 9 AGAGTAC 6645 0.0 19.90759 10-11 CAGAGTA 7205 0.0 19.632149 10-11 CTAGACG 120 0.0050577684 19.588295 4 CGGGTCG 60 0.005069558 19.581068 58-59 >>END_MODULE