FastQCFastQC Report
Wed 25 May 2016
SRR1033158_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033158_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310107
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT29430.1273966963435027No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27970.12107664277022667No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7700.034.1536068
GTACATG228600.031.7756291
TACATGG222700.031.5363832
GTATCAA106200.030.961141
GTACAAG12300.030.6387391
CTGTCGC9550.029.5044279
GGTATCA70850.029.055451
ACATGGG238750.028.4392223
CATGGGG116550.027.2467564
TCAACGC124000.025.9128274
ATCAACG124300.025.8948083
CAACGCA124700.025.654355
TATCAAC127800.025.4540392
CATGGGA115400.025.1165334
AACGCAG129450.024.668166
TTGTACG2402.6557245E-1023.4806069
CTTGCTC18650.022.6755033
TCGCCGG1050.002265948622.49887894
ATGGGAT52050.022.2923875
GTACGGA3051.0913936E-1121.5554986
Produced by FastQC (version 0.11.5)