##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033158_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2310107
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.882169527212376	33.0	31.0	34.0	26.0	34.0
2	30.994202000167093	33.0	31.0	34.0	26.0	34.0
3	31.19947214566252	34.0	31.0	34.0	27.0	34.0
4	34.22895000101727	37.0	35.0	37.0	30.0	37.0
5	34.6208426709239	37.0	35.0	37.0	31.0	37.0
6	34.50903139984425	37.0	35.0	37.0	31.0	37.0
7	34.46069251337708	37.0	35.0	37.0	31.0	37.0
8	34.4491242180557	37.0	35.0	37.0	31.0	37.0
9	35.878911236579086	39.0	38.0	39.0	30.0	39.0
10-11	35.978379356454056	39.0	37.5	39.0	31.0	39.0
12-13	35.92701744984107	39.0	37.5	39.0	30.5	39.0
14-15	37.327275316684464	41.0	38.0	41.0	31.0	41.0
16-17	37.420848904401396	41.0	38.0	41.0	31.0	41.0
18-19	37.49392603892374	41.0	38.5	41.0	31.0	41.0
20-21	37.6220677224042	41.0	39.0	41.0	31.0	41.0
22-23	37.711863346589574	40.5	38.0	41.0	31.0	41.0
24-25	37.839563708520856	40.5	38.0	41.0	30.5	41.0
26-27	37.89704957389419	40.0	38.0	41.0	30.0	41.0
28-29	37.91604371572399	40.0	38.0	41.0	30.0	41.0
30-31	37.91970588375344	40.0	38.0	41.0	30.0	41.0
32-33	37.96604507929719	41.0	38.0	41.0	30.0	41.0
34-35	37.92663803018648	41.0	38.0	41.0	30.0	41.0
36-37	37.82922479348359	41.0	38.0	41.0	30.0	41.0
38-39	37.72744141288693	41.0	38.0	41.0	30.0	41.0
40-41	37.66115314139128	40.5	38.0	41.0	30.0	41.0
42-43	37.52938630115402	40.0	38.0	41.0	30.0	41.0
44-45	37.41576472431797	40.0	38.0	41.0	30.0	41.0
46-47	37.28575018386594	40.0	38.0	41.0	30.0	41.0
48-49	37.159611221471565	40.0	37.0	41.0	30.0	41.0
50-51	37.03333287159426	40.0	37.0	41.0	30.0	41.0
52-53	36.87165486274012	40.0	37.0	41.0	30.0	41.0
54-55	36.68558815673906	40.0	36.0	41.0	30.0	41.0
56-57	36.50263169628073	40.0	36.0	41.0	30.0	41.0
58-59	36.255358734465545	39.0	35.0	41.0	30.0	41.0
60-61	35.968462066908586	39.0	35.0	41.0	29.0	41.0
62-63	35.6380572415044	39.0	35.0	41.0	29.0	41.0
64-65	35.28882168661452	38.0	35.0	40.5	28.5	41.0
66-67	34.91679779334896	37.0	34.5	40.0	28.0	41.0
68-69	34.53133296423066	37.0	34.0	39.5	28.0	41.0
70-71	34.11086607676614	36.0	34.0	39.0	27.5	41.0
72-73	33.70274710219051	36.0	34.0	39.0	27.0	40.5
74-75	33.31766883525309	35.0	34.0	37.5	27.0	40.0
76-77	32.985430328551885	35.0	33.0	37.0	26.5	39.0
78-79	32.62146753375493	35.0	33.0	36.5	26.0	39.0
80-81	32.27876068078232	35.0	33.0	36.0	26.0	38.5
82-83	32.093150663583984	35.0	33.0	36.0	26.0	37.0
84-85	31.848583853475184	35.0	33.0	35.5	25.0	37.0
86-87	31.643986620533163	35.0	33.0	35.0	25.0	36.5
88-89	31.3233339408088	35.0	33.0	35.0	24.0	36.0
90-91	31.142176531216954	35.0	33.0	35.0	23.5	36.0
92-93	30.96361185867148	35.0	33.0	35.0	22.0	36.0
94-95	30.781558170249255	35.0	32.0	35.0	20.0	35.5
96-97	30.608052354284887	35.0	32.0	35.0	18.5	35.0
98-99	30.40386462618398	35.0	32.0	35.0	8.5	35.0
100	30.226010743225313	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2757718631107444
1101	2	0.23386155900048777
1101	3	0.3822982630347802
1101	4	0.37383997318791273
1101	5	0.21638130395761124
1101	6	0.3481231086685952
1101	7	0.17363670213811133
1101	8	0.17986628879079092
1101	9	0.4281301858990574
1101	10-11	0.3455070570137764
1101	12-13	0.3453028532129636
1101	14-15	0.39822326248734186
1101	16-17	0.31712162330907034
1101	18-19	0.2508559011767275
1101	20-21	0.18831371118695728
1101	22-23	0.2577083020878135
1101	24-25	0.21476613218847973
1101	26-27	0.3028518052767595
1101	28-29	0.2861271562291776
1101	30-31	0.2668830234433841
1101	32-33	0.3252806428696573
1101	34-35	0.3410642355716078
1101	36-37	0.2890782238229477
1101	38-39	0.2033670197086508
1101	40-41	0.335565331085391
1101	42-43	0.26311058604605364
1101	44-45	0.17538932235149218
1101	46-47	0.2704652124328888
1101	48-49	0.22142450783971412
1101	50-51	0.13311382755998125
1101	52-53	0.16063737797726674
1101	54-55	0.20548253960042473
1101	56-57	0.28009666360347296
1101	58-59	0.21649753277924333
1101	60-61	0.2490132567988823
1101	62-63	0.24840551949051815
1101	64-65	0.19096393044738136
1101	66-67	0.24080280525468822
1101	68-69	0.3178630734894696
1101	70-71	0.23113121902412104
1101	72-73	0.3214496124866315
1101	74-75	0.23679126378446114
1101	76-77	0.25885316939683634
1101	78-79	0.19101370555083008
1101	80-81	0.11075819607530946
1101	82-83	0.2485573972053814
1101	84-85	0.23349031803764575
1101	86-87	0.27506555062536364
1101	88-89	0.4173890078906801
1101	90-91	0.49696489620652073
1101	92-93	0.43835577699404027
1101	94-95	0.5099878054041085
1101	96-97	0.5039657515980203
1101	98-99	0.6080524309229567
1101	100	0.6400512420753444
1102	1	0.1679249423839373
1102	2	0.12251729143335766
1102	3	0.18659256276142244
1102	4	0.0596098919851471
1102	5	0.014338170013246554
1102	6	0.026295342950632516
1102	7	0.009466920018802227
1102	8	0.08504397009003384
1102	9	0.11972549865030402
1102	10-11	0.025278473238252275
1102	12-13	0.025486313174376107
1102	14-15	0.1162956515370297
1102	16-17	0.04267737986157272
1102	18-19	-0.009619305850129933
1102	20-21	-0.03799955260143406
1102	22-23	0.017786288902421177
1102	24-25	-0.003559764257893505
1102	26-27	0.02535090079051372
1102	28-29	0.03183185151780776
1102	30-31	0.016534796236442162
1102	32-33	0.14096669569165243
1102	34-35	0.14169134603952926
1102	36-37	0.1783552880615744
1102	38-39	0.13895754873261978
1102	40-41	0.19147137506352152
1102	42-43	0.12262553016890365
1102	44-45	0.06518747116963652
1102	46-47	0.08240091351023437
1102	48-49	0.15365386545347803
1102	50-51	0.09595778358184504
1102	52-53	-0.042510234179033546
1102	54-55	8.611173460479904E-4
1102	56-57	-0.05948264979096507
1102	58-59	-0.12857728833347437
1102	60-61	-0.17583398552302754
1102	62-63	-0.1312691669542545
1102	64-65	-0.14451127657947183
1102	66-67	-0.20824245925729912
1102	68-69	-0.17372972679996224
1102	70-71	-0.09488029810081855
1102	72-73	-0.11136112879010085
1102	74-75	-0.03605706392095698
1102	76-77	-0.07114185592877931
1102	78-79	-0.07835410880839078
1102	80-81	-0.017975573421395552
1102	82-83	-0.04327936312586189
1102	84-85	0.012356996764125938
1102	86-87	0.05227174334123319
1102	88-89	0.19790160034927595
1102	90-91	0.2591970677775137
1102	92-93	0.2656288011940724
1102	94-95	0.3213443840341128
1102	96-97	0.28562804658113805
1102	98-99	0.3390187739845416
1102	100	0.3182375461435214
1103	1	0.16557776237718258
1103	2	0.1378161769450088
1103	3	0.23709281605422916
1103	4	0.17189987003310137
1103	5	0.062166184197643304
1103	6	0.2421820788506679
1103	7	0.27659479067060744
1103	8	0.3202854425655488
1103	9	0.2865293655871497
1103	10-11	0.21500481146857453
1103	12-13	0.26703213806461434
1103	14-15	0.2801671265454715
1103	16-17	0.2554792204559675
1103	18-19	0.23375655956696306
1103	20-21	0.20073788829691352
1103	22-23	0.18522972017563433
1103	24-25	0.11425727390488305
1103	26-27	0.27282958197929474
1103	28-29	0.30295225003182225
1103	30-31	0.3011588674961416
1103	32-33	0.38884589613068954
1103	34-35	0.36550931294259925
1103	36-37	0.34523567053371096
1103	38-39	0.37580743052930643
1103	40-41	0.3973261327467341
1103	42-43	0.3309097880209748
1103	44-45	0.25032287728243574
1103	46-47	0.38419612627619415
1103	48-49	0.42293683573378615
1103	50-51	0.354281090741587
1103	52-53	0.30555629739813384
1103	54-55	0.3929235540228433
1103	56-57	0.27445225395777584
1103	58-59	0.2593443386506493
1103	60-61	0.37140512288628713
1103	62-63	0.39671652200217977
1103	64-65	0.2894505395299518
1103	66-67	0.29450576769306025
1103	68-69	0.2907575663442401
1103	70-71	0.23789473567155284
1103	72-73	0.2423960478394207
1103	74-75	0.23746750548127693
1103	76-77	0.2169085282319756
1103	78-79	0.1523602824445618
1103	80-81	0.2637488228974192
1103	82-83	0.2479139626086848
1103	84-85	0.2579950835591305
1103	86-87	0.29004245002816376
1103	88-89	0.34467686630672034
1103	90-91	0.44325967331618443
1103	92-93	0.4209706409441516
1103	94-95	0.3663613757598796
1103	96-97	0.3999480609343955
1103	98-99	0.3885918700669464
1103	100	0.3310974330060681
1104	1	0.024236111065572885
1104	2	0.04381118895782521
1104	3	0.10824267790247077
1104	4	0.0018036887611998509
1104	5	0.13704614353080302
1104	6	0.1365590879132128
1104	7	0.06051218484321197
1104	8	0.14310384343487925
1104	9	0.08185488840275212
1104	10-11	0.19067049252437585
1104	12-13	0.1729415161104768
1104	14-15	-0.005582047081276187
1104	16-17	0.04137844491626197
1104	18-19	0.13966735937727748
1104	20-21	-0.02305910211208584
1104	22-23	-0.07228999204173903
1104	24-25	-8.467533195002375E-4
1104	26-27	0.001435745350093498
1104	28-29	0.08851003228925691
1104	30-31	0.1096533195524998
1104	32-33	0.0991354133082325
1104	34-35	-0.0030733344440605492
1104	36-37	0.14097492680653545
1104	38-39	0.0065993161476995965
1104	40-41	-0.04592517790135986
1104	42-43	-0.06933708497214752
1104	44-45	-0.09499981294089821
1104	46-47	-0.09869616478567167
1104	48-49	-0.18209563080444013
1104	50-51	-0.16042804687295842
1104	52-53	-0.23907759169198783
1104	54-55	-0.20715018086927017
1104	56-57	-0.17987700787961813
1104	58-59	-0.13154666801108306
1104	60-61	-0.08393703676080122
1104	62-63	-0.1698432170406079
1104	64-65	-0.17605362689610615
1104	66-67	-0.16656139995965447
1104	68-69	-0.16882363871706474
1104	70-71	-0.1533377678762946
1104	72-73	-0.06288674767323243
1104	74-75	-0.10818213588410686
1104	76-77	-0.20933000359717369
1104	78-79	-0.10082370437546473
1104	80-81	-0.06539659385892094
1104	82-83	-0.09734977907289988
1104	84-85	-0.052879402199600634
1104	86-87	0.007731048177156197
1104	88-89	0.09413522873154179
1104	90-91	0.05897937923404584
1104	92-93	0.030132075517617096
1104	94-95	0.06230671132254173
1104	96-97	-0.0016155846796586104
1104	98-99	0.04344001031154221
1104	100	0.03643882708767876
1105	1	0.17577934851420807
1105	2	0.3106747278666546
1105	3	0.2113291527997383
1105	4	0.10706178157065693
1105	5	0.2803887621414205
1105	6	0.2077597717210935
1105	7	0.10757189215394192
1105	8	0.24587643264663228
1105	9	0.2188351294409685
1105	10-11	0.2657723124928637
1105	12-13	0.3214878152642413
1105	14-15	0.3677634545673314
1105	16-17	0.3160294348946593
1105	18-19	0.3175843035194319
1105	20-21	0.39434490490465635
1105	22-23	0.36473857033745816
1105	24-25	0.37341472198382775
1105	26-27	0.3959327973422333
1105	28-29	0.49688322734504453
1105	30-31	0.44953832288220497
1105	32-33	0.4384735530584223
1105	34-35	0.4001291725807974
1105	36-37	0.3555338626000619
1105	38-39	0.43597513842262003
1105	40-41	0.39152416590295047
1105	42-43	0.3297512050208695
1105	44-45	0.3683402271779954
1105	46-47	0.4048876341650569
1105	48-49	0.40185244608740334
1105	50-51	0.408032735689126
1105	52-53	0.4061574774197112
1105	54-55	0.3969350027518246
1105	56-57	0.3966541492337683
1105	58-59	0.3949690587581358
1105	60-61	0.3877588392766853
1105	62-63	0.4067173795452277
1105	64-65	0.34327227009416106
1105	66-67	0.4792401252080509
1105	68-69	0.33803555533170027
1105	70-71	0.33642521391589497
1105	72-73	0.34484750931508756
1105	74-75	0.27900519661544365
1105	76-77	0.21869454689253587
1105	78-79	0.3498127973218814
1105	80-81	0.3868664894486926
1105	82-83	0.31134065167734093
1105	84-85	0.305822905855031
1105	86-87	0.3375986111573184
1105	88-89	0.3869336231636247
1105	90-91	0.41672602110388013
1105	92-93	0.3867957508631754
1105	94-95	0.3011135193528176
1105	96-97	0.30174122578698714
1105	98-99	0.43980301727956217
1105	100	0.45238832113481564
1106	1	-0.034027009568013966
1106	2	-0.07658180766574318
1106	3	-0.015190470271608802
1106	4	0.031165822916079833
1106	5	-0.07321813629306462
1106	6	-0.07196854593825464
1106	7	-0.06649967157279235
1106	8	2.0913525519716814E-4
1106	9	-0.026577053902244074
1106	10-11	-0.09165564838337303
1106	12-13	-0.164758619258059
1106	14-15	-0.035116698312819494
1106	16-17	-0.06068222974803916
1106	18-19	-0.026459583627904237
1106	20-21	-0.07239801356239184
1106	22-23	-0.04948097836484777
1106	24-25	-0.03379625074438053
1106	26-27	-0.0341179430533316
1106	28-29	0.016929449115409057
1106	30-31	0.0064278142544509365
1106	32-33	0.02171463112708949
1106	34-35	-0.04532066597248985
1106	36-37	-0.003622001728217583
1106	38-39	-0.006200108925042969
1106	40-41	-0.03257610992145743
1106	42-43	-0.004819101963235539
1106	44-45	-0.025226380181720742
1106	46-47	-0.025247359763035604
1106	48-49	-0.01448614956153449
1106	50-51	0.011465478777047622
1106	52-53	0.043450726781927074
1106	54-55	0.0553937750037079
1106	56-57	0.05795353639523171
1106	58-59	0.001879731186043898
1106	60-61	0.0015684168793654862
1106	62-63	0.025046523736435233
1106	64-65	0.02400880600310984
1106	66-67	0.08646474794991121
1106	68-69	0.029151279206040215
1106	70-71	0.04238509228957099
1106	72-73	0.085410642981671
1106	74-75	0.08340614929225865
1106	76-77	0.11909369211927157
1106	78-79	0.12108752032073511
1106	80-81	0.13455351817020045
1106	82-83	0.1224254041414028
1106	84-85	0.1671524171845462
1106	86-87	0.1772952043021938
1106	88-89	0.2839235598087342
1106	90-91	0.2925960918015349
1106	92-93	0.24385233723010558
1106	94-95	0.2128280552052857
1106	96-97	0.2711995555901474
1106	98-99	0.37277909680736343
1106	100	0.46498116476214335
1107	1	-0.052894197924253206
1107	2	0.002014452828326796
1107	3	-0.005579983872802785
1107	4	0.05883992037119867
1107	5	-0.09987208951636006
1107	6	-0.06710236588959617
1107	7	-0.017130302203426595
1107	8	0.01853139912491031
1107	9	-0.07905288739997474
1107	10-11	-0.014770442009115925
1107	12-13	0.016384275461483355
1107	14-15	0.01884507367815047
1107	16-17	-0.08846110351231573
1107	18-19	0.009160854328300161
1107	20-21	-0.03193178090638327
1107	22-23	-0.08025101847795924
1107	24-25	-0.040334906188149944
1107	26-27	0.07607626575401127
1107	28-29	0.03790443870353499
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2216	30-31	-0.38693103908059356
2216	32-33	-0.29472058790497613
2216	34-35	-0.31505142248099816
2216	36-37	-0.32902853789000375
2216	38-39	-0.2892671621284322
2216	40-41	-0.28235031606875083
2216	42-43	-0.22399019905036255
2216	44-45	-0.2065050511920461
2216	46-47	-0.10912862254496503
2216	48-49	-0.1440346008477036
2216	50-51	-0.1274059288781757
2216	52-53	-0.2039201021729724
2216	54-55	-0.1914881967754809
2216	56-57	-0.2784730979863923
2216	58-59	-0.2939063492029348
2216	60-61	-0.29875410863250806
2216	62-63	-0.2817481848435932
2216	64-65	-0.1732916106221296
2216	66-67	-0.178956169613393
2216	68-69	-0.2045327185766368
2216	70-71	-0.26843738350802226
2216	72-73	-0.13818853507541462
2216	74-75	-0.14341643178265429
2216	76-77	-0.27543780107052385
2216	78-79	-0.2536582382055883
2216	80-81	-0.20486201725269382
2216	82-83	-0.24337633137468018
2216	84-85	-0.2039604940387285
2216	86-87	-0.2852707483633026
2216	88-89	-0.2482197824081851
2216	90-91	-0.3150978930294315
2216	92-93	-0.2263766344379512
2216	94-95	-0.23682901367922682
2216	96-97	-0.28572837428736264
2216	98-99	-0.29254294455882146
2216	100	-0.24722710496614653
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23895.0
3	4175.0
4	3067.0
5	2991.0
6	2399.0
7	4080.0
8	5568.0
9	5373.0
10	5413.0
11	5597.0
12	6355.0
13	7220.0
14	8078.0
15	8347.0
16	7891.0
17	7108.0
18	6308.0
19	5541.0
20	4991.0
21	4949.0
22	6671.0
23	12788.0
24	26181.0
25	38231.0
26	33879.0
27	19039.0
28	15663.0
29	20161.0
30	23893.0
31	28181.0
32	37661.0
33	52427.0
34	74379.0
35	114109.0
36	213791.0
37	502248.0
38	739483.0
39	221546.0
40	430.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.92482064838048	18.739133248578284	12.190480823482043	22.145565279559193
2	16.731583279121786	20.967018431348244	39.301110943046886	23.000287346483084
3	19.492795558820674	28.1285086564185	27.526492521796985	24.852203262963833
4	12.104349181740625	16.779708726512123	38.181727633738525	32.93421445800872
5	12.238180781936704	41.42295745930336	33.038029723226494	13.300832035533444
6	29.82805137591801	38.16235157211902	18.085602863530337	13.923994188432626
7	28.386290159190835	34.17139552159977	20.36531811326048	17.07699620594892
8	26.75264163703132	34.6559485864655	19.69921315887229	18.892196617630887
9	27.60918226413297	17.055510094515665	19.099308559231407	36.23599908211996
10-11	24.251716799009007	28.585490972801175	26.63764581236563	20.52514641582419
12-13	25.299989414239427	27.032287435654705	26.18540494214308	21.482318207962788
14-15	23.274967029864925	27.130437907472338	24.825624137862356	24.76897092480038
16-17	21.458367384492156	30.187312944130845	24.993705722218312	23.360613949158687
18-19	21.458099891065086	28.981449890805305	27.656998765173384	21.903451452956226
20-21	25.149489548937748	27.410949259718226	26.189031861949164	21.250529329394862
22-23	23.897375817114437	27.511826606460453	26.61100644512981	21.9797911312953
24-25	22.907332099019882	28.183654064314773	26.859610411878503	22.04940342478684
26-27	22.237816252674385	27.77856072655337	27.216959146743147	22.766663874029096
28-29	22.38912337602594	27.83131040821977	27.377821882574878	22.401744333179412
30-31	23.062871463104056	28.7788624694051	26.32665935520911	21.831606712281733
32-33	22.080157885826342	28.165867581836373	27.407570500671785	22.346404031665497
34-35	22.606358452327193	27.29460326889098	27.623453649869052	22.47558462891277
36-37	23.050702683887927	28.31957635491013	26.367118671364214	22.26260228983773
38-39	22.659184218626947	28.557002217978393	26.896462447846336	21.887351115548316
40-41	22.133568901993034	27.844362770829463	27.376912525220558	22.64515580195695
42-43	21.95488295395954	27.976261340844182	27.091115947293027	22.977739757903255
44-45	22.19560228427352	27.917347257282948	27.253914812061748	22.63313564638178
46-47	22.362813028297822	28.292346621816144	27.051294539362182	22.293545810523856
48-49	21.977740591461565	28.138742621990275	27.02620509746906	22.857311689079108
50-51	22.078009503724452	27.879359454253393	26.980352057296813	23.06227898472534
52-53	22.35931754036251	27.938562095181652	26.753299209341318	22.94882115511452
54-55	22.24851592001271	28.9565617915366	26.446428749225653	22.348493539225036
56-57	22.294563629799388	28.014371217047024	26.577314446454036	23.113750706699555
58-59	22.0254308165428	27.221094772713105	27.322197268262947	23.431277142481143
60-61	22.470099239550887	28.194127617703163	26.671627670191967	22.664145472553983
62-63	21.980025419517272	28.13845758247923	26.699215942955	23.182301055048494
64-65	21.762053913586662	27.8771636179933	26.858505351727462	23.50227711669257
66-67	21.72911376404616	27.80027116155324	26.449658061125632	24.02095701327497
68-69	22.772221079858443	28.12729299466456	26.355861949546	22.744623975930995
70-71	22.63992978484162	27.570921314218243	26.65680393326097	23.132344967679167
72-73	21.910423894118676	27.81581674572673	26.809694821515308	23.46406453863929
74-75	21.962878580942935	28.100258826574944	26.64333663341906	23.293525959063064
76-77	22.0215969650223	27.95098962099792	26.37003758224295	23.657375831736832
78-79	22.36296682857756	28.12104507523867	26.55313351793527	22.9628545782485
80-81	22.282474590975088	27.72341283962033	26.630558535345795	23.363554034058787
82-83	21.90800881284776	27.796465553682808	27.056801916333356	23.23872371713607
84-85	21.874505551352854	27.71778196965614	26.740605340424175	23.66710713856683
86-87	22.363383829583867	27.872596983731228	26.463205499299903	23.300813687385
88-89	21.96859939413037	27.65025499354649	26.758044344639693	23.623101267683445
90-91	21.900863704254142	27.457053265319548	26.824356904254664	23.817726126171646
92-93	22.290528525709778	27.996127825320933	26.32157561357526	23.39176803539403
94-95	21.738253746043174	27.333915602349023	26.912369384147084	24.015461267460722
96-97	21.61076073124824	27.48057910373402	26.99467563902348	23.913984525994255
98-99	21.73398319289672	27.569785362569466	26.554125761935225	24.14210568259859
100	21.904611765382167	27.419925888796616	26.540198294719413	24.135264051101803
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2103.0
1	1728.0
2	1291.5
3	1042.0
4	704.0
5	464.0
6	338.5
7	324.0
8	392.0
9	415.5
10	345.5
11	293.5
12	301.0
13	317.5
14	354.0
15	405.5
16	481.5
17	616.0
18	826.5
19	1108.0
20	1495.5
21	1990.5
22	2733.0
23	3839.5
24	5084.0
25	6355.0
26	8275.0
27	11099.0
28	14486.0
29	18382.5
30	22251.0
31	26479.0
32	31588.5
33	36455.0
34	41220.5
35	47868.5
36	55690.5
37	62773.5
38	69604.5
39	73536.0
40	76738.5
41	80881.5
42	85151.5
43	90813.5
44	92164.0
45	93237.0
46	97194.0
47	100832.0
48	103850.0
49	101056.0
50	97819.5
51	92221.0
52	81321.0
53	73044.0
54	67625.0
55	65102.5
56	62394.5
57	57333.0
58	51203.0
59	43729.0
60	35340.5
61	27943.5
62	21776.5
63	16527.5
64	12023.0
65	8550.0
66	6338.0
67	4440.5
68	3014.5
69	2133.0
70	1423.0
71	906.0
72	574.0
73	363.0
74	283.0
75	235.5
76	146.5
77	93.0
78	61.5
79	46.5
80	40.5
81	22.5
82	16.0
83	17.5
84	13.0
85	11.0
86	9.5
87	6.0
88	3.0
89	3.0
90	6.0
91	4.5
92	1.5
93	1.5
94	2.5
95	2.5
96	1.5
97	1.5
98	2.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3370406652159402
2	0.12068705042666855
3	0.0662739864430522
4	0.0405608917682168
5	0.046361488883415354
6	0.009999536817991548
7	0.007575406680296627
8	0.015800133933190106
9	0.018916872681655005
10-11	0.021795527220167726
12-13	0.017336859288335994
14-15	0.019090024834347498
16-17	0.020323733922281522
18-19	0.020388665979541205
20-21	0.025150350178584798
22-23	0.016384522448527276
24-25	0.02558323056031604
26-27	0.02002071765506966
28-29	0.019760989426030915
30-31	0.021211138704830556
32-33	0.025539942522142915
34-35	0.017055487040210694
36-37	0.010778721505107773
38-39	0.01497766120790076
40-41	0.010194332989770603
42-43	0.00883075978731721
44-45	0.009783096627125929
46-47	0.0091770640927022
48-49	0.005086344485342021
50-51	0.007380610508517571
52-53	0.008549387539191907
54-55	0.0038959234355811223
56-57	0.0031600267866380215
58-59	0.003246602862984269
60-61	0.003917567454667684
62-63	0.0030734507102917743
64-65	0.00253235023312773
66-67	0.0019479617177905612
68-69	0.0035496191301961338
70-71	0.0027920784621664712
72-73	0.0021211138704830554
74-75	0.0028570105194261567
76-77	0.004610176065437661
78-79	0.003960855492840808
80-81	0.004372091855485482
82-83	0.0030518066912052126
84-85	0.003657839225628943
86-87	0.0029219425766858417
88-89	0.608997765038589
90-91	0.618932369799321
92-93	0.6164000195661932
94-95	0.6024612712744475
96-97	0.600361801423051
98-99	0.5980675353998754
100	0.5985869918579528
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2310107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.78429258430445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.78621182257635	36.236100654783
2	13.454973963491298	13.396927210252988
3	5.358513375153275	8.00309393056618
4	2.711842097912026	5.4002856177954515
5	1.5693579324062967	3.9064687238207085
6	1.0177157931834757	3.0399756489308176
7	0.662822549597506	2.3098706218445826
8	0.483258215492373	1.9246934715072919
9	0.33583064945477203	1.504718218011002
>10	1.434531809682324	12.363500245692778
>50	0.10476348384630965	3.657518663537406
>100	0.07764483567155038	7.186115000083807
>500	0.002184027183220581	0.7072250270524346
>1k	3.4944434931524066E-4	0.3635069661216131
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	2943	0.1273966963435027	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2797	0.12107664277022667	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	4.328803817312358E-5	0.0
5	0.0	0.0	0.0	4.328803817312358E-5	0.0
6	0.0	0.0	0.0	4.328803817312358E-5	0.0
7	0.0	0.0	0.0	4.328803817312358E-5	0.0
8	0.0	0.0	0.0	4.328803817312358E-5	0.0
9	0.0	0.0	0.0	4.328803817312358E-5	0.0
10-11	0.0	0.0	0.0	4.328803817312358E-5	0.0
12-13	0.0	0.0	0.0	4.328803817312358E-5	0.0
14-15	0.0	0.0	0.0	4.328803817312358E-5	0.0
16-17	0.0	0.0	0.0	1.7315215269249432E-4	0.0
18-19	0.0	0.0	0.0	1.947961717790561E-4	0.0
20-21	0.0	0.0	0.0	3.030162672118651E-4	0.0
22-23	0.0	0.0	0.0	8.008287062027863E-4	0.0
24-25	0.0	0.0	0.0	0.0017748095650980669	0.0
26-27	0.0	0.0	0.0	0.0026622143476471003	0.0
28-29	0.0	0.0	0.0	0.005973749267891054	0.0
30-31	0.0	0.0	0.0	0.018159332013625344	0.0
32-33	0.0	0.0	0.0	0.04131843243624646	0.0
34-35	0.0	0.0	0.0	0.07363295293248322	0.0
36-37	0.0	0.0	0.0	0.13008055471023636	0.0
38-39	0.0	0.0	0.0	0.22667781189356162	0.0
40-41	0.0	0.0	0.0	0.3422568738158016	0.0
42-43	0.0	0.0	0.0	0.45095313766851497	0.0
44-45	0.0	0.0	0.0	0.5695190742247004	0.0
46-47	0.0	0.0	0.0	0.7203129552007764	0.0
48-49	0.0	0.0	0.0	0.8904998772784117	0.0
50-51	0.0	0.0	0.0	1.0856207093437664	0.0
52-53	0.0	0.0	0.0	1.2704822763620904	0.0
54-55	0.0	0.0	0.0	1.4498462625324282	0.0
56-57	0.0	0.0	0.0	1.665658776844536	0.0
58-59	0.0	0.0	0.0	1.9175518709739419	0.0
60-61	0.0	0.0	0.0	2.196110396617992	0.0
62-63	0.0	0.0	0.0	2.466097890703764	0.0
64-65	0.0	0.0	0.0	2.733055222117417	0.0
66-67	0.0	0.0	0.0	3.0356169649284643	0.0
68-69	0.0	0.0	0.0	3.391011758329809	0.0
70-71	0.0	0.0	0.0	3.7840022128845114	0.0
72-73	0.0	0.0	0.0	4.197316401361496	0.0
74-75	0.0	0.0	0.0	4.580502115269985	0.0
76-77	0.0	0.0	0.0	4.9632549487967434	0.0
78-79	0.0	0.0	0.0	5.4241859792641645	0.0
80-81	0.0	0.0	0.0	5.950698387563866	0.0
82-83	0.0	0.0	0.0	6.488357465693147	0.0
84-85	0.0	0.0	0.0	6.999740704651343	0.0
86-87	0.0	0.0	0.0	7.5088296775863625	0.0
88	0.0	0.0	0.0	7.9296759847054705	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGTCG	770	0.0	34.153606	8
GTACATG	22860	0.0	31.775629	1
TACATGG	22270	0.0	31.536383	2
GTATCAA	10620	0.0	30.96114	1
GTACAAG	1230	0.0	30.638739	1
CTGTCGC	955	0.0	29.504427	9
GGTATCA	7085	0.0	29.05545	1
ACATGGG	23875	0.0	28.439222	3
CATGGGG	11655	0.0	27.246756	4
TCAACGC	12400	0.0	25.912827	4
ATCAACG	12430	0.0	25.894808	3
CAACGCA	12470	0.0	25.65435	5
TATCAAC	12780	0.0	25.454039	2
CATGGGA	11540	0.0	25.116533	4
AACGCAG	12945	0.0	24.66816	6
TTGTACG	240	2.6557245E-10	23.480606	9
CTTGCTC	1865	0.0	22.675503	3
TCGCCGG	105	0.0022659486	22.498878	94
ATGGGAT	5205	0.0	22.292387	5
GTACGGA	305	1.0913936E-11	21.555498	6
>>END_MODULE