FastQCFastQC Report
Wed 25 May 2016
SRR1033158_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033158_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2310107
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155000.6709645916834156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129560.5608398225709892No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67340.2915016490578142No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32500.14068612406265166No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT31390.13588115182543492No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA113800.047.2256051
AACGCGA405.679077E-446.998056
TATAACG1600.044.07882
ACGCGAG450.001014375641.7760477
GTATCAA203150.040.4001851
TATCAAC254800.032.126092
ATCAACG257700.031.7280713
TCAACGC259450.031.5140634
CAACGCG600.00415431631.3449255
CAACGCA261200.031.2129215
AACGCAG267850.030.4956576
GTACATG218450.030.1598831
TACATGG216150.029.800522
ACATGGG224700.028.0388533
ACGCAGA302350.026.9071047
CGCGAGT700.00884335426.8537048
CGCAGAG304200.026.6175658
CATGGGG106300.026.0961974
CATGGGA109400.025.6145924
GCAGAGT320950.025.0820059
Produced by FastQC (version 0.11.5)