##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033158_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2310107
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.672060211929576	34.0	31.0	34.0	31.0	34.0
2	32.70766895213079	34.0	31.0	34.0	31.0	34.0
3	32.79646397331379	34.0	31.0	34.0	31.0	34.0
4	36.27657593349572	37.0	37.0	37.0	35.0	37.0
5	36.25217663077944	37.0	37.0	37.0	35.0	37.0
6	36.363511300558805	37.0	37.0	37.0	35.0	37.0
7	36.33050676873409	37.0	37.0	37.0	35.0	37.0
8	36.328455781485445	37.0	37.0	37.0	35.0	37.0
9	38.129855456911734	39.0	39.0	39.0	37.0	39.0
10-11	38.105264821066726	39.0	39.0	39.0	36.0	39.0
12-13	38.07113306872799	39.0	39.0	39.0	35.0	39.0
14-15	39.483456177571	41.0	39.0	41.0	36.5	41.0
16-17	39.509544363096595	41.0	39.0	41.0	36.5	41.0
18-19	39.609324373286604	41.0	40.0	41.0	37.0	41.0
20-21	39.62881502891425	41.0	40.0	41.0	37.0	41.0
22-23	39.53252814696462	41.0	39.5	41.0	36.5	41.0
24-25	39.464892102400455	41.0	39.0	41.0	36.0	41.0
26-27	39.33725883692833	41.0	39.0	41.0	36.0	41.0
28-29	39.22864698475006	41.0	39.0	41.0	35.5	41.0
30-31	39.022553717208766	40.0	38.5	41.0	35.0	41.0
32-33	38.8939811013083	41.0	38.0	41.0	35.0	41.0
34-35	38.759514386130164	41.0	38.0	41.0	35.0	41.0
36-37	38.60993711546695	41.0	38.0	41.0	35.0	41.0
38-39	38.46740627165755	41.0	38.0	41.0	35.0	41.0
40-41	38.280785262327676	41.0	38.0	41.0	35.0	41.0
42-43	38.040016544688186	41.0	38.0	41.0	34.5	41.0
44-45	37.687196740237574	41.0	38.0	41.0	33.5	41.0
46-47	37.11200650013181	40.0	38.0	41.0	32.0	41.0
48-49	36.599655773520446	40.0	38.0	41.0	30.5	41.0
50-51	36.33350944350197	40.0	37.0	41.0	29.5	41.0
52-53	36.15552569642878	40.0	37.0	41.0	28.5	41.0
54-55	35.96913281506008	40.0	36.5	41.0	28.0	41.0
56-57	35.778632981069705	40.0	36.0	41.0	28.0	41.0
58-59	35.54203852895126	39.5	35.5	41.0	27.0	41.0
60-61	35.297082775819476	39.0	35.0	41.0	26.0	41.0
62-63	35.01421665749682	39.0	35.0	41.0	26.0	41.0
64-65	34.71201745200547	38.5	35.0	41.0	26.0	41.0
66-67	34.368464101446385	37.0	35.0	40.0	25.5	41.0
68-69	33.986749964395585	37.0	35.0	39.5	24.5	41.0
70-71	33.601106139239434	36.5	35.0	39.0	24.0	41.0
72-73	33.216271800397124	36.0	34.0	39.0	23.0	41.0
74-75	32.87247279022141	35.0	34.0	37.5	22.5	40.0
76-77	32.55030459628061	35.0	34.0	37.0	22.0	39.0
78-79	32.23141006022665	35.0	34.0	37.0	21.0	39.0
80-81	31.92939612753868	35.0	34.0	36.0	20.0	38.5
82-83	31.663628134973834	35.0	34.0	36.0	19.5	37.0
84-85	31.44393008635531	35.0	34.0	35.5	18.5	37.0
86-87	31.271764684492968	35.0	33.5	35.0	18.0	36.5
88-89	31.095789935271398	35.0	33.0	35.0	16.0	36.0
90-91	30.93432771728755	35.0	33.0	35.0	10.5	36.0
92-93	30.803493084952343	35.0	33.0	35.0	6.0	36.0
94-95	30.673035707869808	35.0	33.0	35.0	2.0	35.5
96-97	30.574030553563105	35.0	33.0	35.0	2.0	35.0
98-99	30.48487472658193	35.0	33.0	35.0	2.0	35.0
100	30.386444870302544	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.19140892111585117
1101	2	0.1976967790557822
1101	3	0.20303422869857712
1101	4	0.09173220304388252
1101	5	0.09262847878405012
1101	6	0.12036101089218931
1101	7	0.15677783055829053
1101	8	0.15347167373509052
1101	9	0.22553526953517888
1101	10-11	0.18506832996497025
1101	12-13	0.1697491065242147
1101	14-15	0.19725066214633813
1101	16-17	0.20089157008560932
1101	18-19	0.30988928760925205
1101	20-21	0.25863993736783186
1101	22-23	0.28404057777805747
1101	24-25	0.25510001719288056
1101	26-27	0.2640410725490838
1101	28-29	0.3277728047904773
1101	30-31	0.22884957429641872
1101	32-33	0.1317206998242284
1101	34-35	0.2247331349635786
1101	36-37	0.3673574938362094
1101	38-39	0.3492076351633173
1101	40-41	0.3563092605844176
1101	42-43	0.33387137569461345
1101	44-45	0.2555202201705953
1101	46-47	0.20713854321592606
1101	48-49	0.16979839413540532
1101	50-51	0.20277389116903777
1101	52-53	0.2313825219397927
1101	54-55	0.333531905698095
1101	56-57	0.3420746329039801
1101	58-59	0.37092199142229276
1101	60-61	0.3744055843730152
1101	62-63	0.3389250436904305
1101	64-65	0.43012510503174894
1101	66-67	0.3579748638047633
1101	68-69	0.4056444027702284
1101	70-71	0.4102986609735879
1101	72-73	0.4472251489164307
1101	74-75	0.47500767221395535
1101	76-77	0.4525978846022838
1101	78-79	0.4350178076930078
1101	80-81	0.39095241050973684
1101	82-83	0.4279264865543091
1101	84-85	0.5117351248154165
1101	86-87	0.5033176654950609
1101	88-89	0.544761361670929
1101	90-91	0.4645622648854548
1101	92-93	0.4758963342229059
1101	94-95	0.4792807625972024
1101	96-97	0.4650974290461889
1101	98-99	0.4875756751317155
1101	100	0.4356695092735485
1102	1	0.0694841743720005
1102	2	0.10779134811833302
1102	3	0.06798890388751744
1102	4	0.07000895573724364
1102	5	0.022503157188943135
1102	6	0.09446506701053892
1102	7	0.0959556525553964
1102	8	0.09778748013483352
1102	9	0.09311848227034858
1102	10-11	0.11173710709956453
1102	12-13	0.1112893963618049
1102	14-15	0.13413309903329917
1102	16-17	0.09677841885550009
1102	18-19	0.09409117899050301
1102	20-21	0.15658901479184806
1102	22-23	0.18833864619741547
1102	24-25	0.16200200103273232
1102	26-27	0.21112611435645334
1102	28-29	0.24194861681813506
1102	30-31	0.2944426660314221
1102	32-33	0.18106092735276746
1102	34-35	0.20620398035672594
1102	36-37	0.2125255272821036
1102	38-39	0.21550069875875266
1102	40-41	0.26788618211521253
1102	42-43	0.1738745413965006
1102	44-45	0.14971366968015332
1102	46-47	0.2396173882874777
1102	48-49	0.20471307093232127
1102	50-51	0.15630490499763283
1102	52-53	0.17469133364585332
1102	54-55	0.14492139757781786
1102	56-57	0.13528771981505372
1102	58-59	0.12020744728337007
1102	60-61	0.12299659578965105
1102	62-63	0.0806414571009384
1102	64-65	0.11775700927680077
1102	66-67	0.16761693849522175
1102	68-69	0.1458088685203478
1102	70-71	0.10227209922732072
1102	72-73	0.04939038850478994
1102	74-75	0.10837743624355056
1102	76-77	0.07408169429837841
1102	78-79	0.09678266136723579
1102	80-81	-0.00853253481909988
1102	82-83	0.016740252835059977
1102	84-85	0.04874846096270957
1102	86-87	0.12449043241900526
1102	88-89	0.09082609273701792
1102	90-91	0.07735546917874458
1102	92-93	0.14468452364242523
1102	94-95	0.13208090128509653
1102	96-97	0.09569594769870093
1102	98-99	0.052045947683570404
1102	100	0.029777786327734646
1103	1	0.152648645834347
1103	2	0.13187632785491132
1103	3	0.1293163692708319
1103	4	0.09072514114757269
1103	5	0.021854094372322663
1103	6	0.046809080857208585
1103	7	0.072757197641522
1103	8	0.08375505865560484
1103	9	0.10620527727203921
1103	10-11	0.1176525495176648
1103	12-13	0.09272567211323945
1103	14-15	0.12405521149529619
1103	16-17	0.1773218374640848
1103	18-19	0.09820138246906396
1103	20-21	0.1251179112795313
1103	22-23	0.09875267439735325
1103	24-25	0.09278710319416206
1103	26-27	0.09350854427877664
1103	28-29	0.09324779783806036
1103	30-31	0.10568200662581972
1103	32-33	0.17229171931494136
1103	34-35	0.17650064955632416
1103	36-37	0.1262708835806592
1103	38-39	0.1355261335780682
1103	40-41	0.19638478900477452
1103	42-43	0.20583534433469453
1103	44-45	0.3184768620138243
1103	46-47	0.2689207275309755
1103	48-49	0.19967148869658757
1103	50-51	0.208971867172572
1103	52-53	0.21359288717506786
1103	54-55	0.1644777130117916
1103	56-57	0.14749590539425128
1103	58-59	0.2454375215825877
1103	60-61	0.1741997366204444
1103	62-63	0.16956306297733903
1103	64-65	0.2196150175510354
1103	66-67	0.29696777013993625
1103	68-69	0.3414527599421646
1103	70-71	0.36188931047352924
1103	72-73	0.3768737133950282
1103	74-75	0.3687270858551628
1103	76-77	0.334289922093042
1103	78-79	0.29889449014130065
1103	80-81	0.25688983093571593
1103	82-83	0.30242973696205055
1103	84-85	0.31840978737270476
1103	86-87	0.29039036065270807
1103	88-89	0.2531260969585638
1103	90-91	0.22747103402170765
1103	92-93	0.2613912950034525
1103	94-95	0.2569062347872908
1103	96-97	0.24183692625324227
1103	98-99	0.24488725974030956
1103	100	0.19022083766174447
1104	1	0.05750230908702747
1104	2	0.10706631933307165
1104	3	0.06171551586218271
1104	4	-0.010818260727930351
1104	5	-0.03865652905170691
1104	6	-0.01365057777933032
1104	7	0.02417780281446369
1104	8	0.030336415080142842
1104	9	0.11287236828071201
1104	10-11	0.05903309155552705
1104	12-13	0.05209700286727781
1104	14-15	0.08121679218239564
1104	16-17	0.0749924732596483
1104	18-19	0.00832279240443512
1104	20-21	0.05241964950688072
1104	22-23	0.05231658949045226
1104	24-25	0.041225338683851476
1104	26-27	-0.0954819023389959
1104	28-29	-0.02521279653822006
1104	30-31	0.09237642394737833
1104	32-33	0.06456517317889876
1104	34-35	0.06498258571078708
1104	36-37	0.043113466257352684
1104	38-39	0.04624785621413707
1104	40-41	0.02534696219580468
1104	42-43	0.014799088028624396
1104	44-45	-0.006445476836830721
1104	46-47	0.048651462841299065
1104	48-49	0.06652762188223704
1104	50-51	0.07185794184277228
1104	52-53	0.03607230658770533
1104	54-55	-4.981476150476283E-4
1104	56-57	0.01616621002806795
1104	58-59	-0.007318459452378079
1104	60-61	-0.039132793962799894
1104	62-63	-0.03817870697505299
1104	64-65	0.014386435010834475
1104	66-67	0.0789448386103615
1104	68-69	0.10238000409892578
1104	70-71	0.05654401200797565
1104	72-73	0.11345378804422523
1104	74-75	0.20081500820669618
1104	76-77	0.15815714766625177
1104	78-79	0.11158008508628825
1104	80-81	0.12580648360347269
1104	82-83	0.07477943677288579
1104	84-85	0.06836111928165067
1104	86-87	0.03154911837179597
1104	88-89	0.01584498306516835
1104	90-91	0.06106930769342611
1104	92-93	0.03692689554801021
1104	94-95	0.018768617174561797
1104	96-97	0.06749628592552881
1104	98-99	0.03028968688910183
1104	100	0.06027397228052678
1105	1	0.05772868847858348
1105	2	0.0839004999817945
1105	3	0.07510641520761396
1105	4	0.0607036178758662
1105	5	0.040144005325480236
1105	6	0.04654948881478305
1105	7	-0.029308845877203282
1105	8	-0.07213589151267996
1105	9	0.01992099010733739
1105	10-11	8.937982683931978E-5
1105	12-13	0.03933874763314549
1105	14-15	0.006761320907209267
1105	16-17	0.03059013515643727
1105	18-19	2.7155131220979456E-5
1105	20-21	-0.006699582386829661
1105	22-23	0.09521832662235141
1105	24-25	0.01321510186867414
1105	26-27	0.02207722024289893
1105	28-29	0.04720048898811058
1105	30-31	0.09012633461379949
1105	32-33	0.08344359018801839
1105	34-35	0.041814939081909586
1105	36-37	0.08093374741446269
1105	38-39	0.08765924543238413
1105	40-41	0.133469820220391
1105	42-43	0.10753931412376971
1105	44-45	0.32961777539244963
1105	46-47	0.2939957625473184
1105	48-49	0.17951388075468344
1105	50-51	0.14882602133339873
1105	52-53	0.18296806649154718
1105	54-55	0.1692835536210069
1105	56-57	0.1241205524517639
1105	58-59	0.10890258834919564
1105	60-61	0.15517614995196283
1105	62-63	0.2190002606537078
1105	64-65	0.10722442045757674
1105	66-67	0.10269345800062268
1105	68-69	0.15377157750676673
1105	70-71	0.13789938501625443
1105	72-73	0.08648438885310128
1105	74-75	-0.0072383117536674035
1105	76-77	0.03316770527016644
1105	78-79	0.10736151020039131
1105	80-81	0.1317180311857804
1105	82-83	0.11093598167518692
1105	84-85	0.09643926991429197
1105	86-87	0.06252868128699518
1105	88-89	0.04502051541204466
1105	90-91	0.18289197649326994
1105	92-93	0.10474417184862617
1105	94-95	0.0035036416194742515
1105	96-97	0.016947798099259614
1105	98-99	0.010552347892559766
1105	100	-0.02612499145004321
1106	1	0.05552021040803368
1106	2	0.06620914091112695
1106	3	0.11131660659021492
1106	4	0.06964296474625087
1106	5	0.04852605508683894
1106	6	0.05551048743095777
1106	7	0.023198520423264313
1106	8	0.05018988253723222
1106	9	0.047923287075150256
1106	10-11	0.0754101551476154
1106	12-13	0.1350178122777166
1106	14-15	0.15657116209636257
1106	16-17	0.09792800594259177
1106	18-19	0.1278421174289477
1106	20-21	0.07448034362067801
1106	22-23	0.10843530535657919
1106	24-25	0.1262240355672688
1106	26-27	0.12267829153362442
1106	28-29	0.03897376696828303
1106	30-31	0.032613649514907195
1106	32-33	0.031051542968377532
1106	34-35	0.08054238125762936
1106	36-37	0.046459461216045383
1106	38-39	0.028155541101099857
1106	40-41	-0.01725833740431426
1106	42-43	-0.10647924989478952
1106	44-45	-0.06717353001954507
1106	46-47	-0.056933850451265755
1106	48-49	-0.13602454168029254
1106	50-51	-0.05417745235973115
1106	52-53	-0.13720806575354771
1106	54-55	-0.16924902284260668
1106	56-57	-0.16484835375852214
1106	58-59	-0.1643833935574719
1106	60-61	-0.1398787795857288
1106	62-63	-0.13044150085701745
1106	64-65	-0.12474955979226365
1106	66-67	-0.1219429338771505
1106	68-69	-0.1022532561042695
1106	70-71	-0.2113305659378426
1106	72-73	-0.0769891159997087
1106	74-75	-0.045784538230925875
1106	76-77	-0.12765910900493083
1106	78-79	-0.12471696325739146
1106	80-81	-0.15657119848276224
1106	82-83	-0.15166721713490716
1106	84-85	-0.10936433933759204
1106	86-87	-0.12502627178519887
1106	88-89	-0.12031786371943909
1106	90-91	-0.0602618356260578
1106	92-93	-0.09797688776898639
1106	94-95	-0.11753903114733433
1106	96-97	-0.07778847422321888
1106	98-99	-0.08281704030441617
1106	100	-0.01231117763622791
1107	1	-0.013654511759384036
1107	2	0.047155196455719306
1107	3	-0.0019294862179179972
1107	4	-0.010817787977273952
1107	5	-0.039169085309033846
1107	6	-0.05777286891809297
1107	7	-0.06963285109423367
1107	8	-0.004459072987636148
1107	9	-0.060823123569065274
1107	10-11	-0.0725649001997013
1107	12-13	-0.014053010369742935
1107	14-15	0.0632810106310231
1107	16-17	0.01470953199419256
1107	18-19	0.07491057274557278
1107	20-21	0.028863193623969607
1107	22-23	0.03743510807900918
1107	24-25	0.01390361295486997
1107	26-27	0.058193733983380014
1107	28-29	0.023211289162013315
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2216	6	-0.0488191397508686
2216	7	-0.07048627018859577
2216	8	-0.06800034837751667
2216	9	-0.1454121388073375
2216	10-11	-0.08833795782144449
2216	12-13	-0.06636541470601998
2216	14-15	-0.17618941713684677
2216	16-17	-0.09574856394042541
2216	18-19	-0.06339032904046604
2216	20-21	-0.0907258926957013
2216	22-23	-0.12545755742613807
2216	24-25	-0.11066568904156782
2216	26-27	-0.12579778679529596
2216	28-29	-0.11127977590590632
2216	30-31	-0.08823668578035182
2216	32-33	-0.13996453911673257
2216	34-35	-0.09301955982216725
2216	36-37	-0.1551822800707967
2216	38-39	-0.17450082658449162
2216	40-41	-0.24728316855443921
2216	42-43	-0.19903344659311273
2216	44-45	-0.08272545935636799
2216	46-47	-0.08635375943187285
2216	48-49	-0.13510131808880743
2216	50-51	-0.08815073531591366
2216	52-53	-0.05832851535268446
2216	54-55	-0.05145189223439672
2216	56-57	-0.041694362285980446
2216	58-59	-0.12829260681747456
2216	60-61	0.0043297006971911856
2216	62-63	-0.06100695053088856
2216	64-65	-0.023908868419219687
2216	66-67	-0.08109572440843493
2216	68-69	-0.024811780870116706
2216	70-71	0.0013171066211086213
2216	72-73	-0.02700503756940975
2216	74-75	-0.06550203759568518
2216	76-77	-0.08907123526232397
2216	78-79	-0.12169420451076718
2216	80-81	-0.12129845549523921
2216	82-83	-0.08161068581457798
2216	84-85	-0.08238030054875267
2216	86-87	-0.0490867529545298
2216	88-89	-0.07750924683594818
2216	90-91	-0.10975624455565125
2216	92-93	-0.09769341884236837
2216	94-95	-0.06414432503676792
2216	96-97	-0.025077160875682125
2216	98-99	-0.023834318131907395
2216	100	-0.04915031064502173
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4.0
3	7.0
4	22.0
5	52.0
6	101.0
7	309.0
8	528.0
9	973.0
10	1762.0
11	3341.0
12	5997.0
13	10173.0
14	10569.0
15	14131.0
16	20203.0
17	34783.0
18	43191.0
19	19330.0
20	9711.0
21	7405.0
22	6617.0
23	6352.0
24	7032.0
25	8054.0
26	9527.0
27	11780.0
28	14154.0
29	17653.0
30	22242.0
31	28654.0
32	36821.0
33	47957.0
34	67949.0
35	103587.0
36	187570.0
37	484110.0
38	810010.0
39	256578.0
40	868.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.659035019158544	18.14104885906552	12.89432730184203	21.305588819933902
2	17.182845811167823	20.57159850966394	39.647579762285595	22.59797591688264
3	19.244476554549205	27.915719921198455	29.06008249834315	23.77972102590919
4	11.766439952486987	16.462851888919285	39.063349528852385	32.70735862974134
5	11.487653660727162	39.608968715505235	33.927890155450285	14.97548746831732
6	29.414446022536655	37.743078988252904	17.678908689352813	15.163566299857626
7	26.96105402825547	34.29596167957074	21.154418083156024	17.58856620901777
8	27.577711970169272	31.81930140716791	20.045790205709622	20.557196416953197
9	28.77579157657336	13.553799502620013	20.414310172048456	37.25609874875817
10-11	24.78700000952342	26.248585555392985	27.22776648915585	21.736647945927743
12-13	27.032189418065915	25.049121101316953	27.455892735704452	20.46279674491268
14-15	24.08426280006199	24.732674604541955	27.754895229915853	23.4281673654802
16-17	20.225513363666707	28.470824078711505	27.589003453086807	23.714659104534984
18-19	19.85747401092158	25.667924185264308	33.17219347172531	21.302408332088802
20-21	23.174792336458875	24.284372974931465	32.45769568249436	20.0831390061153
22-23	21.93000568806053	24.504632262990985	32.902356692475045	20.663005356473438
24-25	20.99040952683969	25.21984931420866	33.06713331212217	20.722607846829476
26-27	20.487941184555382	24.847456152369176	33.45378404626519	21.210818616810243
28-29	20.614602906883995	24.955988935597844	33.515229137846354	20.914179019671806
30-31	21.238416892073438	25.790294947460175	32.55892854397798	20.41235961648841
32-33	20.385426891337737	25.218796234275747	33.432774686532426	20.963002187854094
34-35	20.97312257807793	24.502322685373716	33.42952283899981	21.09503189754854
36-37	21.439804256833668	25.451915670407786	32.15205340244904	20.9562266703095
38-39	21.15806683324681	25.681211327161122	32.48728458922956	20.673437250362507
40-41	20.82947692487554	25.10622037331116	32.640381386618344	21.423921315194953
42-43	20.834431090473878	25.29580991962578	32.036186693689714	21.833572296210633
44-45	21.275566765461722	25.297732764967712	31.71364573237617	21.7130547371944
46-47	21.74585042637076	25.925480959162655	30.67875783270784	21.649910781758738
48-49	21.90318916886536	26.20030810904298	29.619725385964262	22.2767773361274
50-51	22.085129189055337	26.284834117477967	29.050372562499245	22.57966413096745
52-53	22.416411778444107	26.45379360471723	28.645893646297328	22.483900970541335
54-55	22.264556966409067	27.239778045980394	28.35335762163922	22.142307365971323
56-57	22.161223259594188	26.182205421293624	28.625001758614232	23.031569560497953
58-59	21.792971821617936	25.309095568499274	29.45945963495306	23.438472974929727
60-61	22.087939392863746	26.225763400662572	28.90431589058613	22.781981315887556
62-63	21.615810409454593	26.06629747198465	29.029645383500867	23.288246735059886
64-65	21.414700284021425	25.93685728843304	29.09353832860686	23.554904098938675
66-67	21.49784186551474	25.807898975670717	28.70771227355388	23.986546885260662
68-69	22.522203709210952	26.1220681299016	28.402133772450995	22.953594388436453
70-71	22.51451343469398	25.645783452758124	28.428497282153465	23.41120583039443
72-73	22.140684074874745	25.866002221550776	28.42180288455295	23.57151081902153
74-75	22.27304504793093	26.16807966771929	28.21232460136921	23.34655068298057
76-77	22.467117772581755	26.002721526617567	27.924585964982906	23.605574735817772
78-79	22.85520169741092	26.16312278243449	28.058728028339818	22.92294749181477
80-81	22.74130895567647	25.83981990339352	28.150916835184375	23.267954305745633
82-83	22.2924165456399	25.922851657615816	28.503914341185105	23.280817455559184
84-85	22.091268323607565	25.87571406525387	28.158926419002118	23.874091192136447
86-87	22.477201233018615	26.05459583403857	27.910010938906186	23.55819199403663
88-89	21.953983083900443	25.85663780941749	28.155427432582126	24.033951674099942
90-91	21.74587584038315	25.60182277271139	28.290464467663185	24.36183691924227
92-93	21.78414246612819	26.196708637305544	27.881544015060772	24.13760488150549
94-95	21.34424942221291	25.494728166271084	28.374876142100774	24.786146269415227
96-97	21.05763932146866	25.622449522900887	28.48415679446883	24.835754361161626
98-99	21.18259024365538	25.61935442817151	27.969873256953033	25.22818207122008
100	21.291394727603528	25.413151858333833	28.033246944838485	25.262206469224154
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	349.0
1	232.0
2	334.5
3	560.5
4	365.0
5	249.0
6	567.5
7	1233.5
8	2142.5
9	2542.5
10	2057.5
11	1606.0
12	1687.5
13	1964.0
14	2334.0
15	2770.5
16	3116.5
17	3538.0
18	3986.0
19	4401.5
20	4946.5
21	5399.0
22	6076.5
23	7139.5
24	8167.0
25	9254.5
26	10985.0
27	13278.5
28	16242.5
29	19685.0
30	23229.5
31	26978.0
32	31475.0
33	36322.5
34	40820.5
35	47064.0
36	54764.0
37	61292.0
38	67620.5
39	71924.0
40	75691.0
41	79801.0
42	83725.5
43	89432.5
44	90600.0
45	91549.5
46	95765.0
47	99513.5
48	102536.0
49	99644.0
50	96294.5
51	90286.0
52	78226.0
53	69301.0
54	64267.5
55	62105.5
56	59981.0
57	55724.0
58	50052.5
59	42826.5
60	34477.0
61	26811.5
62	20513.5
63	15542.5
64	11292.5
65	7892.0
66	5860.5
67	4090.5
68	2729.0
69	1895.0
70	1195.0
71	727.0
72	448.0
73	275.0
74	194.0
75	138.5
76	79.5
77	44.5
78	21.5
79	13.0
80	6.5
81	2.0
82	2.5
83	3.0
84	2.0
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08653278830807404
2	0.001038912916154966
3	0.0
4	1.2986411451937075E-4
5	0.038093473592348756
6	1.7315215269249432E-4
7	0.004848260275389841
8	2.597282290387415E-4
9	8.657607634624716E-5
10-11	5.627444962506066E-4
12-13	0.0
14-15	4.328803817312358E-5
16-17	0.0
18-19	8.657607634624716E-5
20-21	0.0
22-23	2.1644019086561791E-4
24-25	8.657607634624716E-5
26-27	0.00145014927879964
28-29	0.007640338737556313
30-31	0.03305041714517986
32-33	0.023288964537140487
34-35	0.026124331037480082
36-37	0.02380842099521797
38-39	0.02199032339194678
40-41	0.02768270041171253
42-43	0.020496886074974015
44-45	0.019198244929780308
46-47	0.013917104272659232
48-49	0.009112132035442514
50-51	0.00688279806952665
52-53	0.004653464103610785
54-55	0.003419755015676763
56-57	0.0021427578895696175
58-59	8.874047825490334E-4
60-61	6.926086107699773E-4
62-63	0.001471793297886202
64-65	0.0030301626721186505
66-67	4.3288038173123583E-4
68-69	8.657607634624717E-4
70-71	2.597282290387415E-4
72-73	3.895923435581122E-4
74-75	5.19456458077483E-4
76-77	2.813722481253033E-4
78-79	2.164401908656179E-5
80-81	5.843885153371684E-4
82-83	5.19456458077483E-4
84-85	2.813722481253033E-4
86-87	1.7315215269249432E-4
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2310107.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.31155108718624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.01399569124797	30.581171958035853
2	14.9856878355694	13.280781442049175
3	6.140758289302724	8.163195739515
4	3.1857594162509746	5.646637644987586
5	1.866046864139885	4.134371547570909
6	1.158083017395742	3.0789872873120525
7	0.789237965098611	2.4480650887289803
8	0.544723419147588	1.9310031692756087
9	0.37405559751118717	1.491748534670845
>10	1.707273569524454	13.070258296516313
>50	0.13186842645997207	4.151944781990523
>100	0.09643105364573301	8.115238568385383
>500	0.004412071963818754	1.3157971581976105
>1k	0.0013726446109657996	1.061943800363982
>5k	9.804604364041426E-5	0.29256349677417076
>10k+	1.960920872808285E-4	1.2362914856260474
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15500	0.6709645916834156	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12956	0.5608398225709892	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6734	0.2915016490578142	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3250	0.14068612406265166	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	3139	0.13588115182543492	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.328803817312358E-5	0.0
10-11	0.0	0.0	0.0	4.328803817312358E-5	0.0
12-13	0.0	0.0	0.0	8.657607634624716E-5	0.0
14-15	0.0	0.0	0.0	8.657607634624716E-5	0.0
16-17	0.0	0.0	0.0	2.1644019086561791E-4	0.0
18-19	0.0	0.0	0.0	2.3808420995217973E-4	0.0
20-21	0.0	0.0	0.0	3.4630430538498864E-4	0.0
22-23	0.0	0.0	0.0	8.441167443759099E-4	0.0
24-25	0.0	0.0	0.0	0.0018180976032711905	0.0
26-27	0.0	0.0	0.0	0.0027487904239933475	0.0
28-29	0.0	0.0	0.0	0.005973749267891054	0.0
30-31	0.0	0.0	0.0	0.01813768799453878	0.0
32-33	0.0	0.0	0.0	0.04146994056985239	0.0
34-35	0.0	0.0	0.0	0.07380610508517571	0.0
36-37	0.0	0.0	0.0	0.1302320628438423	0.0
38-39	0.0	0.0	0.0	0.22682932002716757	0.0
40-41	0.0	0.0	0.0	0.3425815341021	0.0
42-43	0.0	0.0	0.0	0.45186218647015053	0.0
44-45	0.0	0.0	0.0	0.5708610034080672	0.0
46-47	0.0	0.0	0.0	0.7225206451476057	0.0
48-49	0.0	0.0	0.0	0.8935083959314438	0.0
50-51	0.0	0.0	0.0	1.0896681409129534	0.0
52-53	0.0	0.0	0.0	1.2759365691719042	0.0
54-55	0.0	0.0	0.0	1.4562528921820506	0.0
56-57	0.0	0.0	0.0	1.6735804878302174	0.0
58-59	0.0	0.0	0.0	1.9267072910475576	0.0
60-61	0.0	0.0	0.0	2.20695405018036	0.0
62-63	0.0	0.0	0.0	2.4792141662702205	0.0
64-65	0.0	0.0	0.0	2.7476000029435865	0.0
66-67	0.0	0.0	0.0	3.0508327103463175	0.0
68-69	0.0	0.0	0.0	3.4085650578090103	0.0
70-71	0.0	0.0	0.0	3.8046289630740047	0.0
72-73	0.0	0.0	0.0	4.22051878982229	0.0
74-75	2.164401908656179E-5	0.0	0.0	4.605998769753955	0.0
76-77	4.328803817312358E-5	0.0	0.0	4.990677920979418	0.0
78-79	4.328803817312358E-5	0.0	0.0	5.455851179187803	0.0
80-81	8.657607634624716E-5	0.0	0.0	5.9889866573279935	0.0
82-83	8.657607634624716E-5	0.0	0.0	6.5346540225193035	0.0
84-85	8.657607634624716E-5	0.0	0.0	7.052725263375246	0.0
86-87	8.657607634624716E-5	0.0	0.0	7.568740322417966	0.0
88	8.657607634624716E-5	0.0	0.0	7.995040922346887	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11380	0.0	47.225605	1
AACGCGA	40	5.679077E-4	46.99805	6
TATAACG	160	0.0	44.0788	2
ACGCGAG	45	0.0010143756	41.776047	7
GTATCAA	20315	0.0	40.400185	1
TATCAAC	25480	0.0	32.12609	2
ATCAACG	25770	0.0	31.728071	3
TCAACGC	25945	0.0	31.514063	4
CAACGCG	60	0.004154316	31.344925	5
CAACGCA	26120	0.0	31.212921	5
AACGCAG	26785	0.0	30.495657	6
GTACATG	21845	0.0	30.159883	1
TACATGG	21615	0.0	29.80052	2
ACATGGG	22470	0.0	28.038853	3
ACGCAGA	30235	0.0	26.907104	7
CGCGAGT	70	0.008843354	26.853704	8
CGCAGAG	30420	0.0	26.617565	8
CATGGGG	10630	0.0	26.096197	4
CATGGGA	10940	0.0	25.614592	4
GCAGAGT	32095	0.0	25.082005	9
>>END_MODULE