FastQCFastQC Report
Wed 25 May 2016
SRR1033157_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033157_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821426
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23800.029.7041171
GTACATG72350.028.206661
TACATGG71150.027.5568432
GTATCAA34350.027.0297361
TCAACGC32250.026.9488684
ATCAACG33200.026.4677813
ACATGGG73750.025.422893
CAACGCA34550.025.2912965
AACGCAG36450.024.478526
TATCAAC40800.021.8958452
CATGGGG48750.021.1041564
GTCTCGC4700.021.0583211
ACGCAGA43350.020.5822857
GTCTTAC11250.019.6900861
GAGTACA50650.019.6338441
CGCAGAG45000.019.6188898
ATACCGT4303.6379788E-1217.4735286
TCTGTCG6250.017.281328
GTATTAG12100.017.1383741
GTCTAGG8950.016.851141
Produced by FastQC (version 0.11.5)