##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033157_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2821426
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.569234493479538	33.0	31.0	34.0	30.0	34.0
2	31.63746842908515	34.0	31.0	34.0	30.0	34.0
3	31.827527640278355	34.0	31.0	34.0	30.0	34.0
4	35.18276715391437	37.0	35.0	37.0	33.0	37.0
5	35.29523475008737	37.0	35.0	37.0	33.0	37.0
6	35.34575317587631	37.0	36.0	37.0	33.0	37.0
7	35.29253646914716	37.0	36.0	37.0	33.0	37.0
8	35.30807931875584	37.0	36.0	37.0	33.0	37.0
9	36.96445201823475	39.0	38.0	39.0	34.0	39.0
10-11	36.95376114773168	39.0	38.0	39.0	34.0	39.0
12-13	36.90202294867915	39.0	38.0	39.0	34.0	39.0
14-15	38.34883087488383	41.0	39.0	41.0	34.0	41.0
16-17	38.35337662586224	41.0	39.0	41.0	34.0	41.0
18-19	38.33720448454079	41.0	39.0	41.0	34.0	41.0
20-21	38.36668939748907	41.0	39.0	41.0	34.0	41.0
22-23	38.32115284965829	41.0	39.0	41.0	34.0	41.0
24-25	38.31381896955653	41.0	39.0	41.0	34.0	41.0
26-27	38.273973869950865	40.5	39.0	41.0	34.0	41.0
28-29	38.24020406702142	40.0	38.5	41.0	33.5	41.0
30-31	38.23342363046204	40.0	39.0	41.0	34.0	41.0
32-33	38.299894273321364	41.0	39.0	41.0	34.0	41.0
34-35	38.272524780022586	41.0	39.0	41.0	34.0	41.0
36-37	38.19019566701377	41.0	39.0	41.0	33.0	41.0
38-39	38.11196908938955	41.0	38.0	41.0	33.0	41.0
40-41	38.0492467284274	41.0	38.0	41.0	33.0	41.0
42-43	37.936990904599305	40.5	38.0	41.0	33.0	41.0
44-45	37.80479161955692	40.0	38.0	41.0	33.0	41.0
46-47	37.681413405845134	40.0	38.0	41.0	32.5	41.0
48-49	37.564194488886116	40.0	38.0	41.0	32.0	41.0
50-51	37.44184554193518	40.0	37.5	41.0	32.0	41.0
52-53	37.29510786389577	40.0	37.0	41.0	31.5	41.0
54-55	37.144813119323345	40.0	37.0	41.0	31.0	41.0
56-57	36.98990847181532	40.0	36.5	41.0	31.0	41.0
58-59	36.79444082531316	40.0	36.0	41.0	31.0	41.0
60-61	36.576000043949406	39.5	35.5	41.0	31.0	41.0
62-63	36.31823340395955	39.0	35.0	41.0	30.0	41.0
64-65	36.0529099469559	39.0	35.0	41.0	30.0	41.0
66-67	35.76156188395514	38.0	35.0	40.5	30.0	41.0
68-69	35.45925322868649	37.5	35.0	40.0	29.5	41.0
70-71	35.11732418287774	37.0	35.0	40.0	29.0	41.0
72-73	34.756610487037406	36.5	34.0	39.0	29.0	41.0
74-75	34.38970683618851	36.0	34.0	39.0	29.0	41.0
76-77	34.05728946993471	35.5	34.0	39.0	29.0	40.0
78-79	33.68790834847343	35.0	34.0	37.5	28.0	39.5
80-81	33.326325765765255	35.0	34.0	37.0	28.0	39.0
82-83	33.09478451676564	35.0	34.0	36.5	28.0	39.0
84-85	32.815765502976156	35.0	34.0	36.0	27.5	37.5
86-87	32.54963146295526	35.0	34.0	36.0	27.0	37.0
88-89	32.17653661659033	35.0	34.0	35.5	26.0	37.0
90-91	31.978476132282044	35.0	33.0	35.0	26.0	36.0
92-93	31.79143418966154	35.0	33.0	35.0	26.0	36.0
94-95	31.61417985089809	35.0	33.0	35.0	25.0	36.0
96-97	31.435956321377915	35.0	33.0	35.0	25.0	36.0
98-99	31.23683449433017	35.0	33.0	35.0	24.5	35.5
100	31.071804116074638	35.0	33.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.1539322517866175
1101	2	0.17051179128423755
1101	3	0.24607522779784574
1101	4	0.22375198053860856
1101	5	0.20438989742586244
1101	6	0.22874677671157428
1101	7	0.18797768127794967
1101	8	0.1250214045246807
1101	9	0.20617813287719144
1101	10-11	0.22843720081686314
1101	12-13	0.22074435165183104
1101	14-15	0.2775695284365156
1101	16-17	0.21017386444576402
1101	18-19	0.21246130825809217
1101	20-21	0.2023840930308225
1101	22-23	0.1829763418243644
1101	24-25	0.21919484388967447
1101	26-27	0.23349782581176015
1101	28-29	0.3117789216606184
1101	30-31	0.2765885496043339
1101	32-33	0.26759905644562565
1101	34-35	0.29561926894838564
1101	36-37	0.2887730863124176
1101	38-39	0.3020652231225327
1101	40-41	0.29853084231354643
1101	42-43	0.3077390877906012
1101	44-45	0.2803392119201007
1101	46-47	0.35008200535142464
1101	48-49	0.31335708384050776
1101	50-51	0.21283479094660152
1101	52-53	0.2050880967969917
1101	54-55	0.2491696037801745
1101	56-57	0.27160973916481623
1101	58-59	0.22040690216822156
1101	60-61	0.13451180328323886
1101	62-63	0.24068077306519342
1101	64-65	0.22949696600172587
1101	66-67	0.2996960599041145
1101	68-69	0.21009823240163428
1101	70-71	0.29034209150722035
1101	72-73	0.337713492250586
1101	74-75	0.3568160491477741
1101	76-77	0.24876896620866518
1101	78-79	0.23250542834323795
1101	80-81	0.3156474840642147
1101	82-83	0.29745205649474116
1101	84-85	0.22150543208789486
1101	86-87	0.2783106619345901
1101	88-89	0.45795142909558706
1101	90-91	0.44406135335206187
1101	92-93	0.4534894997974419
1101	94-95	0.4514238522152496
1101	96-97	0.43658505660908276
1101	98-99	0.42524544206611736
1101	100	0.41072631672669146
1102	1	0.18137614311808292
1102	2	0.14412439956855394
1102	3	0.14863704254672072
1102	4	0.1649007598546035
1102	5	0.1108468521822985
1102	6	0.06665372184572504
1102	7	0.07508698636080169
1102	8	0.09675342868938941
1102	9	0.22883739509715184
1102	10-11	0.13392953865449897
1102	12-13	0.18062885735589163
1102	14-15	0.1544996370269871
1102	16-17	0.16141470140932057
1102	18-19	0.16036358355761848
1102	20-21	0.18207751233217806
1102	22-23	0.23001749019994833
1102	24-25	0.20421944463226538
1102	26-27	0.25749720944742194
1102	28-29	0.18493612013666194
1102	30-31	0.16087650748700355
1102	32-33	0.2714215919639571
1102	34-35	0.1997248543128336
1102	36-37	0.18459415628322517
1102	38-39	0.10175619984468653
1102	40-41	0.1853367763679401
1102	42-43	0.13884381993454298
1102	44-45	0.10705376121377697
1102	46-47	0.13779741324869121
1102	48-49	0.044641880500840614
1102	50-51	0.042276113196145104
1102	52-53	0.026897132859687645
1102	54-55	0.046463252939673794
1102	56-57	0.04894808819659602
1102	58-59	0.019848776184964834
1102	60-61	-0.01868302975334757
1102	62-63	0.01285148580038964
1102	64-65	-0.02859429629360477
1102	66-67	-0.11592073508207079
1102	68-69	-0.13075555332697775
1102	70-71	-0.1069130012367765
1102	72-73	-0.019257413468551476
1102	74-75	-0.12186433902718363
1102	76-77	-0.12318948437520305
1102	78-79	-0.10029497861872727
1102	80-81	-0.04682275751561349
1102	82-83	-0.03892191785750043
1102	84-85	-0.07846058712705428
1102	86-87	-0.07953681346130992
1102	88-89	0.12967568141738184
1102	90-91	0.15789533467541617
1102	92-93	0.12629222149010388
1102	94-95	0.131319036633343
1102	96-97	0.13776698233045437
1102	98-99	0.16244646162424559
1102	100	0.19322708718211246
1103	1	0.036466906890776585
1103	2	-8.678922350746632E-4
1103	3	0.0010093334175707014
1103	4	0.06062985045354452
1103	5	-0.04228942221288179
1103	6	0.028863115175852272
1103	7	-0.035908102499085714
1103	8	-0.005569444113547206
1103	9	-0.05716230973610692
1103	10-11	0.015021681149761434
1103	12-13	0.04225587137376152
1103	14-15	0.07245465540463414
1103	16-17	0.060563956752190506
1103	18-19	0.026614301509191307
1103	20-21	0.07432625483880884
1103	22-23	0.0736434219397637
1103	24-25	3.595964450866518E-4
1103	26-27	0.10993275033408167
1103	28-29	0.11415995938706658
1103	30-31	0.11505493769827524
1103	32-33	0.08757622100261386
1103	34-35	0.11017743053708529
1103	36-37	0.11891016486222838
1103	38-39	0.09664342634426504
1103	40-41	0.16860293051781383
1103	42-43	0.06202811323061752
1103	44-45	0.009273357602005206
1103	46-47	0.032261279920213326
1103	48-49	0.07833506809080149
1103	50-51	0.028067156866121934
1103	52-53	-0.07366211866901295
1103	54-55	-0.023475675656172257
1103	56-57	-0.037866455729258064
1103	58-59	-0.026367499257993643
1103	60-61	-0.0930443483436818
1103	62-63	-0.016708920200827038
1103	64-65	-0.10525405018076128
1103	66-67	-0.15299610546203013
1103	68-69	-0.1655641773069192
1103	70-71	-0.1565446817002467
1103	72-73	-0.0950371296356991
1103	74-75	-0.1017458187197775
1103	76-77	-0.15407510598549834
1103	78-79	-0.18685981275992702
1103	80-81	-0.17092740243066373
1103	82-83	-0.1220110997409094
1103	84-85	-0.15418372966895788
1103	86-87	-0.11027127392269875
1103	88-89	-0.011718930320157028
1103	90-91	0.03934813003861137
1103	92-93	0.031621163100112426
1103	94-95	0.010521696711787598
1103	96-97	0.007130208004589633
1103	98-99	0.08899051270470792
1103	100	0.14406585266958416
1104	1	0.17068176117829736
1104	2	0.08193970215556945
1104	3	0.15464044594502369
1104	4	0.10954682309894537
1104	5	0.10685095689073165
1104	6	0.12705287738027238
1104	7	0.12215319852818851
1104	8	0.17143478072726026
1104	9	0.17770358418105303
1104	10-11	0.18388172062529407
1104	12-13	0.18334965761679456
1104	14-15	0.057438220814518104
1104	16-17	0.12210636302057765
1104	18-19	0.1864254001143948
1104	20-21	0.06355880512035128
1104	22-23	0.1190551637624111
1104	24-25	0.08138447234031077
1104	26-27	0.12493132324772915
1104	28-29	0.16794045096257548
1104	30-31	0.22489297663894803
1104	32-33	0.19456944920992214
1104	34-35	0.14899384071213717
1104	36-37	0.2702301612307707
1104	38-39	0.24022032437220275
1104	40-41	0.21915929710298343
1104	42-43	0.2470032959709627
1104	44-45	0.24942861745306288
1104	46-47	0.19732549757446805
1104	48-49	0.08213778821471607
1104	50-51	0.07489351266900002
1104	52-53	0.1081097263768811
1104	54-55	0.12554071877596584
1104	56-57	0.1728823562536803
1104	58-59	0.13352690251073085
1104	60-61	0.169675536592635
1104	62-63	0.16938113911102448
1104	64-65	0.11533306558840195
1104	66-67	0.14961808023215184
1104	68-69	0.16706689444664136
1104	70-71	0.15921110927083504
1104	72-73	0.18367727065728445
1104	74-75	0.17398047708017828
1104	76-77	0.1070962947850731
1104	78-79	0.12080734881889299
1104	80-81	0.1644215081733691
1104	82-83	0.24083937252797227
1104	84-85	0.13829798890517964
1104	86-87	0.0993542233864062
1104	88-89	0.22977071994698406
1104	90-91	0.23579189489074537
1104	92-93	0.17983190353558598
1104	94-95	0.1749670380724453
1104	96-97	0.09877803881484937
1104	98-99	0.1801721537591625
1104	100	0.26194478721195935
1105	1	0.023173426294679444
1105	2	-0.03204757660342494
1105	3	0.04282382638867688
1105	4	-0.05133051844563852
1105	5	-0.04413354417740578
1105	6	-0.05489777901612314
1105	7	-0.14284654881411285
1105	8	-0.11136667598243832
1105	9	-0.0669694800200702
1105	10-11	-0.058947061118651334
1105	12-13	-0.011669903908391177
1105	14-15	-0.041241148368968084
1105	16-17	7.086131170268573E-4
1105	18-19	-0.052063963352686926
1105	20-21	-0.005235047394172909
1105	22-23	0.01070500192194146
1105	24-25	-0.04848710807428347
1105	26-27	-0.02149340402594646
1105	28-29	0.05826177277265998
1105	30-31	0.052851964979701904
1105	32-33	0.038385662376306584
1105	34-35	0.0258570873416204
1105	36-37	0.047499067464002565
1105	38-39	-0.04759049432959728
1105	40-41	-0.06238982653106717
1105	42-43	-0.02131211608347172
1105	44-45	-0.07308181202525077
1105	46-47	-0.07420680220380405
1105	48-49	-0.06733732803009929
1105	50-51	-0.08428748922953133
1105	52-53	-0.1149555227860688
1105	54-55	-0.12550130868005738
1105	56-57	-0.14070887042587543
1105	58-59	-0.12980871830011154
1105	60-61	-0.08960176618933247
1105	62-63	-0.05516644773479129
1105	64-65	-0.08409001385828674
1105	66-67	-0.08960541566330704
1105	68-69	-0.1226113348379414
1105	70-71	-0.15159233212300194
1105	72-73	-0.15757605523545948
1105	74-75	-0.17418507065826105
1105	76-77	-0.13239203150877898
1105	78-79	-0.10174180765175578
1105	80-81	-0.15356569529303243
1105	82-83	-0.1441313954807768
1105	84-85	-0.18113007600344133
1105	86-87	-0.10916287067357189
1105	88-89	-0.12684621303554877
1105	90-91	-0.14174295806632387
1105	92-93	-0.17546987337544095
1105	94-95	-0.1584183013798537
1105	96-97	-0.15303493605767216
1105	98-99	-0.19540705140167702
1105	100	-0.16906068889876735
1106	1	0.03206099327168488
1106	2	-0.026242251561285457
1106	3	-0.02955890983980325
1106	4	-0.08411925025932732
1106	5	-0.04497582882623874
1106	6	0.011229239525015089
1106	7	-0.11660920927604934
1106	8	-0.05543009543498556
1106	9	-0.08070155704881898
1106	10-11	-0.09511065133870034
1106	12-13	-0.1460384871723548
1106	14-15	-0.09297904782556543
1106	16-17	-0.11858280310026004
1106	18-19	-0.13873962956625974
1106	20-21	-0.16582270644314434
1106	22-23	-0.12670422722535335
1106	24-25	-0.14113717354630495
1106	26-27	-0.13269590311899293
1106	28-29	-0.06864562847027145
1106	30-31	-0.09120818681842024
1106	32-33	-0.10119870897059258
1106	34-35	-0.17957148337501394
1106	36-37	-0.140630302882208
1106	38-39	-0.09865243671172408
1106	40-41	-0.09729452032127028
1106	42-43	-0.15026174470916231
1106	44-45	-0.1671123340695715
1106	46-47	-0.12814190194314534
1106	48-49	-0.11505202000468273
1106	50-51	-0.10562674504020464
1106	52-53	-0.1319627579506033
1106	54-55	-0.06963919893965453
1106	56-57	-0.04702292452260082
1106	58-59	-0.12089133468439428
1106	60-61	-0.11392039228392292
1106	62-63	-0.010304355776760588
1106	64-65	0.040693865063850865
1106	66-67	0.0479523020437469
1106	68-69	0.034488173903916675
1106	70-71	-0.02812754922607752
1106	72-73	0.07734943941210304
1106	74-75	0.15107563662979118
1106	76-77	0.07057367918680058
1106	78-79	0.07279502640225388
1106	80-81	0.13863555189760746
1106	82-83	0.07531419512822168
1106	84-85	-0.012196108333625943
1106	86-87	0.019369579853176333
1106	88-89	0.11809744569311675
1106	90-91	0.1251124189468058
1106	92-93	0.11363780552184011
1106	94-95	0.18441941353638214
1106	96-97	0.03927615415756236
1106	98-99	0.014003923590511391
1106	100	0.08858355539130613
1107	1	0.026631936152337943
1107	2	-0.03061314226226486
1107	3	-0.03894847831997339
1107	4	-0.009803722433851192
1107	5	0.031509864557158096
1107	6	0.033594179480871844
1107	7	0.0085014604671656
1107	8	0.023175247750785388
1107	9	0.014245007722109904
1107	10-11	-0.0032062334558418115
1107	12-13	-0.009574251837882741
1107	14-15	-0.06890353237182012
1107	16-17	-0.04675434699333891
1107	18-19	0.07794052609404645
1107	20-21	-0.03631716596860457
1107	22-23	-0.01766267913114916
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2216	12-13	-0.30092220861824615
2216	14-15	-0.344979031934713
2216	16-17	-0.3404711890662
2216	18-19	-0.3343882886922884
2216	20-21	-0.32274142731068167
2216	22-23	-0.32378001440469006
2216	24-25	-0.31588266119042174
2216	26-27	-0.41615626781779724
2216	28-29	-0.3102646348927607
2216	30-31	-0.3761319421303142
2216	32-33	-0.34184925097806484
2216	34-35	-0.3720983749502409
2216	36-37	-0.34142006909817724
2216	38-39	-0.4160077163002214
2216	40-41	-0.2984224291328985
2216	42-43	-0.30051743964023814
2216	44-45	-0.2951792227400958
2216	46-47	-0.19095595418858125
2216	48-49	-0.2108559475586489
2216	50-51	-0.20749368020072012
2216	52-53	-0.15276633442756093
2216	54-55	-0.134132646549169
2216	56-57	-0.25478849857601915
2216	58-59	-0.19910473333529666
2216	60-61	-0.2070549334946321
2216	62-63	-0.11952778636089079
2216	64-65	-0.04632362115822275
2216	66-67	-0.1508027881109868
2216	68-69	-0.1102369956200846
2216	70-71	-0.1275048864288948
2216	72-73	-0.06524690997827065
2216	74-75	-0.044341034502330956
2216	76-77	-0.13684668453637272
2216	78-79	-0.20246473363106787
2216	80-81	-0.15606380422715915
2216	82-83	-0.18687427483751406
2216	84-85	-0.16003205115463714
2216	86-87	-0.20289921653287735
2216	88-89	-0.16083203919560418
2216	90-91	-0.2112940084972159
2216	92-93	-0.0851650033898963
2216	94-95	-0.11164835763088377
2216	96-97	-0.1522182014479725
2216	98-99	-0.16231152501753954
2216	100	-0.19540531879341216
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	33205.0
3	5463.0
4	3467.0
5	2866.0
6	2437.0
7	2599.0
8	3093.0
9	3532.0
10	3745.0
11	4414.0
12	4636.0
13	5169.0
14	5844.0
15	6090.0
16	5878.0
17	5982.0
18	5698.0
19	5945.0
20	6030.0
21	6485.0
22	7388.0
23	9483.0
24	13857.0
25	19076.0
26	20646.0
27	18797.0
28	20324.0
29	25116.0
30	31058.0
31	37417.0
32	48889.0
33	66864.0
34	92669.0
35	139412.0
36	258944.0
37	553837.0
38	916477.0
39	417485.0
40	1109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.2629452744103	19.70345266586772	12.966789116759067	24.066812942962912
2	15.24768889462949	19.87153192256431	40.9446044401043	23.936174742701905
3	18.05988729017637	25.219798342335697	30.003369236389172	26.71694513109876
4	11.729138345392256	16.43286324449938	39.60514381711565	32.2328545929927
5	12.355388823980979	38.281561642912735	36.384982102555284	12.978067430550999
6	30.863015990494745	38.633073583498515	17.795096796513228	12.708813629493514
7	27.391498218516368	32.34640463568553	22.80926428199759	17.45283286380051
8	24.967777383906416	37.122509748316205	20.29755405884438	17.612158808933
9	24.95587437161525	16.594958458632156	21.08820410908061	37.360963060671985
10-11	23.66536398326175	26.975401688289036	29.405596339251655	19.95363798919756
12-13	24.26589667297713	24.25701676600878	29.346479613169272	22.130606947844814
14-15	21.45206940166868	26.496732262473984	26.127401351052427	25.923796984804916
16-17	21.340370625938426	29.80906820982769	26.678569586438826	22.17199157779506
18-19	21.14338129400765	28.911752063845153	28.591941365970815	21.35292527617638
20-21	22.370620921729106	28.231082459863398	28.05953185367538	21.338764764732115
22-23	21.924466514047985	28.023211132041624	28.152049611233565	21.900272742676822
24-25	21.84604083661366	28.123036300728693	28.020225980628766	22.01069688202888
26-27	21.615084477876014	28.111978273496646	28.164639023938044	22.108298224689296
28-29	21.629335317106875	28.157708578491192	28.224088420430952	21.988867683970977
30-31	21.823554122582966	28.66976973375188	27.819587254264206	21.687088889400947
32-33	21.473429553498516	28.286126389428794	28.420985646033092	21.819458411039598
34-35	21.612315986099336	28.04306532769305	28.421847231308288	21.922771454899326
36-37	21.9455697169453	28.409841524885877	27.897110718484203	21.74747803968462
38-39	21.75779211756165	28.60691173801583	28.002014139386098	21.63328200503641
40-41	21.44915946794217	28.307155267187127	28.38838073494249	21.85530452992821
42-43	21.494874585568873	28.12490418481727	28.343234309394294	22.036986920219558
44-45	21.605778148544555	28.291525480202345	28.313271696015196	21.7894246752379
46-47	21.704481269219446	28.514760659343892	28.154080615029393	21.62667745640727
48-49	21.59084484789647	28.37696275695211	28.113149933267017	21.9190424618844
50-51	21.58227009078265	28.220919565978697	28.203125996900656	21.993684346338
52-53	21.586407909460736	28.224277433699996	28.241096344890828	21.948218311948445
54-55	21.773668319480333	28.63164289584013	28.014403031456037	21.5802857532235
56-57	21.644120818243103	28.322739159313837	28.031301903123385	22.001838119319675
58-59	21.448307382745636	27.98169177900481	28.47303361806054	22.09696722018902
60-61	21.751525119929905	28.36940165393304	28.050067069301043	21.82900615683601
62-63	21.527338666001263	28.394879476222744	28.133674918975014	21.94410693880097
64-65	21.565767631508944	28.133838216912917	28.25418727348158	22.046206878096562
66-67	21.45652127682198	28.172100177927113	28.129993123932262	22.241385421318647
68-69	21.858844078723045	28.340618303256544	28.007885631406985	21.792651986613425
70-71	21.79102705423252	28.102570736911503	28.126495284205898	21.97990692465008
72-73	21.5531382182749	28.187520424416725	28.256476090761325	22.002865266547055
74-75	21.646851831963964	28.250538479342136	28.12233839505687	21.98027129363703
76-77	21.61563102279524	28.267871779535238	28.000974723643697	22.11552247402583
78-79	21.7193040099655	28.343618363444094	28.08569068491903	21.85138694167138
80-81	21.60381466357899	28.10484157523942	28.22948165993726	22.06186210124433
82-83	21.463810222222378	28.18671330133698	28.3261317313653	22.02334474507534
84-85	21.468495318110957	28.11036771220024	28.23495343807868	22.18618353161013
86-87	21.69008148904022	28.243602998694602	28.0727634641579	21.99355204810728
88-89	21.409458483188097	28.215129037342273	28.143705101516126	22.2317073779535
90-91	21.45527368159966	28.04526491042219	28.211701524622868	22.28775988335529
92-93	21.560279590702493	28.258802380240432	28.06263028585459	22.11828774320249
94-95	21.351622191585886	28.00950443479081	28.276870147121564	22.36200322650174
96-97	21.34299496066243	27.96482037142451	28.32058865424354	22.371596013669517
98-99	21.390363844942282	27.998438334258864	28.241387885859346	22.369809934939507
100	21.54371870620027	27.907785196755835	28.139185051783294	22.409311045260598
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	273.0
1	270.0
2	253.0
3	221.5
4	190.0
5	172.5
6	168.0
7	163.0
8	165.5
9	169.5
10	173.0
11	177.0
12	196.0
13	256.0
14	278.5
15	311.0
16	429.0
17	604.0
18	869.0
19	1256.0
20	1876.5
21	2853.0
22	4397.5
23	6870.0
24	9860.5
25	13402.0
26	18481.5
27	24927.0
28	32782.0
29	41288.5
30	49306.5
31	57497.0
32	65708.5
33	73316.5
34	81049.5
35	87973.5
36	92851.0
37	97229.0
38	100584.0
39	101328.5
40	101944.5
41	102328.5
42	100787.0
43	99659.0
44	98738.0
45	97335.5
46	95913.0
47	93384.5
48	90821.5
49	89103.0
50	86133.0
51	82202.5
52	77143.5
53	72576.0
54	69594.0
55	66468.5
56	62041.0
57	56302.0
58	50647.5
59	45719.0
60	40924.5
61	34835.0
62	29256.0
63	24387.0
64	19853.5
65	16114.0
66	12702.5
67	9515.0
68	6959.0
69	5228.0
70	3671.5
71	2487.0
72	1852.5
73	1360.0
74	1001.0
75	779.5
76	513.5
77	326.5
78	229.5
79	154.5
80	104.0
81	61.0
82	41.0
83	30.0
84	25.5
85	24.0
86	21.5
87	14.0
88	10.0
89	9.0
90	8.0
91	7.0
92	8.0
93	8.5
94	6.0
95	4.0
96	3.0
97	2.0
98	1.0
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.33302308832483996
2	0.11674947349319104
3	0.06365575421790258
4	0.0392709218671693
5	0.04221269670017927
6	0.008860767569307152
7	0.006450638790455606
8	0.015098747938099386
9	0.01782786434944599
10-11	0.02062786690134705
12-13	0.01596710315989149
14-15	0.018217738122495504
16-17	0.01947596711733712
18-19	0.019086093344287604
20-21	0.024243060069624366
22-23	0.01602026776530733
24-25	0.024721541518366953
26-27	0.019121536414564835
28-29	0.019121536414564835
30-31	0.019883562425525247
32-33	0.02507597222113924
34-35	0.014513937278525115
36-37	0.009126590596386366
38-39	0.013113936002574584
40-41	0.009445578228881424
42-43	0.008488615331396252
44-45	0.008701273753059624
46-47	0.008063298488069507
48-49	0.003810130054802075
50-51	0.006450638790455606
52-53	0.006982284844614035
54-55	0.00267595180593076
56-57	0.002268356497742631
58-59	0.002498736454544617
60-61	0.003207597860089189
62-63	0.002622787200514917
64-65	0.0017544319787228162
66-67	0.0016303812327525158
68-69	0.0032607624655050316
70-71	0.001984811935524802
72-73	0.0017012673733069732
74-75	0.0019493688652475734
76-77	0.004022788476465447
78-79	0.003278484000643646
80-81	0.003632914703415932
82-83	0.0022329134274654025
84-85	0.00267595180593076
86-87	0.0023037995680198595
88-89	0.6040916898050844
90-91	0.615132206196441
92-93	0.6115878991687183
94-95	0.5968790250036684
96-97	0.5951423145600842
98-99	0.5929094011326188
100	0.5939195286355198
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2821426.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.11134637095036
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4877119948908	52.674160255525116
2	13.377300737997244	17.95537326672206
3	3.98458075802842	8.022317381852076
4	1.6350167595627332	4.389127042932937
5	0.7996516902338064	2.6832850779698445
6	0.4486167314435622	1.8064363711027507
7	0.26792496821663564	1.258656374038875
8	0.1956880345150282	1.0506309971990835
9	0.1370637118061798	0.8278677214121386
>10	0.6300092705434553	7.272724433665265
>50	0.029183212852774934	1.3382227203653334
>100	0.007252129909365697	0.7211983572144508
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	3.544307027722861E-5	0.0
7	0.0	0.0	0.0	7.088614055445722E-5	0.0
8	0.0	0.0	0.0	7.088614055445722E-5	0.0
9	0.0	0.0	0.0	7.088614055445722E-5	0.0
10-11	0.0	0.0	0.0	7.088614055445722E-5	0.0
12-13	0.0	0.0	0.0	7.088614055445722E-5	0.0
14-15	0.0	0.0	0.0	7.088614055445722E-5	0.0
16-17	0.0	0.0	0.0	7.088614055445722E-5	0.0
18-19	0.0	0.0	0.0	7.088614055445722E-5	0.0
20-21	0.0	0.0	0.0	1.5949381624752874E-4	0.0
22-23	0.0	0.0	0.0	1.7721535138614305E-4	0.0
24-25	0.0	0.0	0.0	2.4810149194060026E-4	0.0
26-27	0.0	0.0	0.0	3.189876324950575E-4	0.0
28-29	0.0	0.0	0.0	8.683552217921009E-4	0.0
30-31	0.0	0.0	0.0	0.0034202562817525605	0.0
32-33	0.0	0.0	0.0	0.00995950274790124	0.0
34-35	0.0	0.0	0.0	0.018377231938743036	0.0
36-37	0.0	0.0	0.0	0.03547851334750584	0.0
38-39	0.0	0.0	0.0	0.06562284461828877	0.0
40-41	0.0	0.0	0.0	0.10117224410634906	0.0
42-43	0.0	0.0	0.0	0.1367925297349638	0.0
44-45	0.0	0.0	0.0	0.17654193305087568	0.0
46-47	0.0	0.0	0.0	0.2258609653416393	0.0
48-49	0.0	0.0	0.0	0.28379266370976947	0.0
50-51	0.0	0.0	0.0	0.3475015825330879	0.0
52-53	0.0	0.0	0.0	0.41238012267555485	0.0
54-55	0.0	0.0	0.0	0.48351436472195264	0.0
56-57	0.0	0.0	0.0	0.5668764660139944	0.0
58-59	0.0	0.0	0.0	0.6616866790055809	0.0
60-61	0.0	0.0	0.0	0.7627348723659596	0.0
62-63	0.0	0.0	0.0	0.8645982563427146	0.0
64-65	0.0	0.0	0.0	0.9747375972292025	0.0
66-67	0.0	3.544307027722861E-5	0.0	1.0978136587668788	0.0
68-69	0.0	3.544307027722861E-5	0.0	1.2399049275082883	0.0
70-71	0.0	3.544307027722861E-5	0.0	1.3979632994095894	0.0
72-73	0.0	3.544307027722861E-5	0.0	1.563021677690643	0.0
74-75	0.0	3.544307027722861E-5	0.0	1.7229230892463598	0.0
76-77	0.0	3.544307027722861E-5	0.0	1.8941485617556513	0.0
78-79	0.0	3.544307027722861E-5	0.0	2.0986905203255377	0.0
80-81	0.0	3.544307027722861E-5	0.0	2.3215211031584735	0.0
82-83	0.0	3.544307027722861E-5	0.0	2.5606909413892125	0.0
84-85	0.0	3.544307027722861E-5	0.0	2.788448110990683	3.544307027722861E-5
86-87	0.0	3.544307027722861E-5	0.0	3.0264660494374125	3.544307027722861E-5
88	0.0	3.544307027722861E-5	0.0	3.2286156007635856	3.544307027722861E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2380	0.0	29.704117	1
GTACATG	7235	0.0	28.20666	1
TACATGG	7115	0.0	27.556843	2
GTATCAA	3435	0.0	27.029736	1
TCAACGC	3225	0.0	26.948868	4
ATCAACG	3320	0.0	26.467781	3
ACATGGG	7375	0.0	25.42289	3
CAACGCA	3455	0.0	25.291296	5
AACGCAG	3645	0.0	24.47852	6
TATCAAC	4080	0.0	21.895845	2
CATGGGG	4875	0.0	21.104156	4
GTCTCGC	470	0.0	21.058321	1
ACGCAGA	4335	0.0	20.582285	7
GTCTTAC	1125	0.0	19.690086	1
GAGTACA	5065	0.0	19.633844	1
CGCAGAG	4500	0.0	19.618889	8
ATACCGT	430	3.6379788E-12	17.473528	6
TCTGTCG	625	0.0	17.28132	8
GTATTAG	1210	0.0	17.138374	1
GTCTAGG	895	0.0	16.85114	1
>>END_MODULE