FastQCFastQC Report
Wed 25 May 2016
SRR1033157_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033157_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2821426
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44040.15609128150091478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36910.13082037239325078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA39900.044.7184751
GTATCAA67950.038.3831831
TCAACGC75400.033.6717074
ATCAACG76500.033.310463
CAACGCA77650.032.696035
AACGCAG80550.031.6216146
TATCAAC83600.030.76322
GTACATG70800.029.3241861
TACATGG71650.027.1665572
ACGCAGA93100.027.106597
CGCAGAG95150.026.4219288
ACATGGG72550.025.6625923
GCAGAGT106050.023.307449
TATACCG1754.4291883E-621.4928845
CCTATAC11950.021.245583
CATGGGG47650.020.6217784
GAGTACT73850.019.78942712-13
AGAGTAC96050.019.66745410-11
CAGAGTA105500.019.53154810-11
GAGTACA52900.019.3118881
Produced by FastQC (version 0.11.5)