##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033157_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2821426
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.72782309371219	34.0	31.0	34.0	31.0	34.0
2	32.80849471153948	34.0	31.0	34.0	31.0	34.0
3	32.914651669049626	34.0	31.0	34.0	31.0	34.0
4	36.35233849833382	37.0	37.0	37.0	35.0	37.0
5	36.291513936569665	37.0	37.0	37.0	35.0	37.0
6	36.376915077694754	37.0	37.0	37.0	35.0	37.0
7	36.341120766591075	37.0	37.0	37.0	35.0	37.0
8	36.33339240511713	37.0	37.0	37.0	35.0	37.0
9	38.1478688436273	39.0	39.0	39.0	37.0	39.0
10-11	38.12757697703218	39.0	39.0	39.0	37.0	39.0
12-13	38.092557274229414	39.0	39.0	39.0	37.0	39.0
14-15	39.61126767102877	41.0	40.0	41.0	37.0	41.0
16-17	39.622399984972134	41.0	40.0	41.0	37.0	41.0
18-19	39.64107990073104	41.0	40.0	41.0	37.0	41.0
20-21	39.63953617780513	41.0	40.0	41.0	37.0	41.0
22-23	39.571732521072676	41.0	40.0	41.0	37.0	41.0
24-25	39.53009364768029	41.0	39.5	41.0	37.0	41.0
26-27	39.42939775843846	41.0	39.0	41.0	36.0	41.0
28-29	39.344640972331014	41.0	39.0	41.0	36.0	41.0
30-31	39.2581441086883	41.0	39.0	41.0	36.0	41.0
32-33	39.30582124074847	41.0	39.0	41.0	36.0	41.0
34-35	39.25973178102137	41.0	39.0	41.0	35.0	41.0
36-37	39.178097883836045	41.0	39.0	41.0	35.0	41.0
38-39	39.09485380796802	41.0	39.0	41.0	35.0	41.0
40-41	38.989774142578966	41.0	39.0	41.0	35.0	41.0
42-43	38.88717921363168	41.0	39.0	41.0	35.0	41.0
44-45	38.7574588169245	41.0	39.0	41.0	35.0	41.0
46-47	38.5656042370064	41.0	39.0	41.0	35.0	41.0
48-49	38.397650868745096	41.0	38.0	41.0	35.0	41.0
50-51	38.2745088830967	40.5	38.0	41.0	34.0	41.0
52-53	38.14377197913396	40.0	38.0	41.0	34.0	41.0
54-55	38.01244778349671	40.0	38.0	41.0	34.0	41.0
56-57	37.85916607417668	40.0	37.0	41.0	34.0	41.0
58-59	37.67426365249345	40.0	37.0	41.0	33.5	41.0
60-61	37.48734965935665	40.0	36.5	41.0	33.0	41.0
62-63	37.257417171316916	39.5	36.0	41.0	33.0	41.0
64-65	37.01106319995633	39.0	35.5	41.0	33.0	41.0
66-67	36.73472244177235	39.0	35.0	41.0	32.5	41.0
68-69	36.415767948548	38.5	35.0	41.0	32.0	41.0
70-71	36.08357351920625	37.0	35.0	40.0	32.0	41.0
72-73	35.731348615912665	37.0	35.0	39.5	31.0	41.0
74-75	35.39453400514492	36.5	35.0	39.0	31.0	41.0
76-77	35.05269728853424	36.0	35.0	39.0	31.0	40.5
78-79	34.7090370259578	35.5	35.0	37.5	31.0	39.5
80-81	34.36420519269334	35.0	35.0	37.0	31.0	39.0
82-83	34.03913127617028	35.0	34.0	37.0	30.5	39.0
84-85	33.76009843958339	35.0	34.0	36.0	30.0	38.0
86-87	33.51695738254344	35.0	34.0	36.0	30.0	37.0
88-89	33.296324801713745	35.0	34.0	36.0	30.0	37.0
90-91	33.09262213504802	35.0	34.0	35.0	30.0	36.5
92-93	32.92392605724906	35.0	34.0	35.0	30.0	36.0
94-95	32.76808234559404	35.0	34.0	35.0	29.5	36.0
96-97	32.63916650658213	35.0	34.0	35.0	29.0	36.0
98-99	32.52980460944218	35.0	34.0	35.0	29.0	36.0
100	32.42980925248438	35.0	34.0	35.0	29.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.13784420459021618
1101	2	0.1621815573597729
1101	3	0.1791876828709178
1101	4	0.1441768897287048
1101	5	0.09664165335436792
1101	6	0.13925626715894168
1101	7	0.15188439986821578
1101	8	0.10345096212589056
1101	9	0.2031024080948356
1101	10-11	0.18544209535751577
1101	12-13	0.16977868150591036
1101	14-15	0.25857887705697635
1101	16-17	0.24911584050185098
1101	18-19	0.28012597970564457
1101	20-21	0.2526588110641512
1101	22-23	0.277295293867752
1101	24-25	0.23596857397349424
1101	26-27	0.24784656569713803
1101	28-29	0.20581764135108216
1101	30-31	0.2887987672654049
1101	32-33	0.20217157263480345
1101	34-35	0.20009869217391696
1101	36-37	0.2359522437981525
1101	38-39	0.21771410742841368
1101	40-41	0.24971550414294086
1101	42-43	0.18469754289147033
1101	44-45	0.24213618136818127
1101	46-47	0.22058526097108455
1101	48-49	0.23482797246754927
1101	50-51	0.21549523384548763
1101	52-53	0.261730685516838
1101	54-55	0.2487617968203324
1101	56-57	0.2389915261824811
1101	58-59	0.1892354329568846
1101	60-61	0.19529852343608667
1101	62-63	0.20314487897581301
1101	64-65	0.19376328614350058
1101	66-67	0.21646292218650132
1101	68-69	0.24869021207723563
1101	70-71	0.23219658916411134
1101	72-73	0.32502359508389134
1101	74-75	0.24384556337045638
1101	76-77	0.2522740257897098
1101	78-79	0.24207117175399162
1101	80-81	0.2741727983987303
1101	82-83	0.2725780573048908
1101	84-85	0.29487980911029865
1101	86-87	0.27972412115350664
1101	88-89	0.21847623615525436
1101	90-91	0.264212912352491
1101	92-93	0.18065400384881514
1101	94-95	0.25519772981477473
1101	96-97	0.3286480794008071
1101	98-99	0.3275775355566424
1101	100	0.3414168133257931
1102	1	0.07709651965735986
1102	2	0.0941520929911519
1102	3	0.10538336912800617
1102	4	0.01615909321775888
1102	5	0.08159751870702081
1102	6	0.08027363167654045
1102	7	0.08951956601070776
1102	8	0.11195658813269915
1102	9	0.1391706899590588
1102	10-11	0.09968653764872215
1102	12-13	0.11876615504463217
1102	14-15	0.13891742660904072
1102	16-17	0.16209791345085733
1102	18-19	0.17548269047330933
1102	20-21	0.20310268086324612
1102	22-23	0.13922944650808944
1102	24-25	0.1770654064260242
1102	26-27	0.15076100679061
1102	28-29	0.16074377856347155
1102	30-31	0.17178714516403204
1102	32-33	0.16606974736198055
1102	34-35	0.23569326170802185
1102	36-37	0.2261047132255385
1102	38-39	0.21334754290597147
1102	40-41	0.230836452859549
1102	42-43	0.22337756770753714
1102	44-45	0.23671813023685218
1102	46-47	0.23367028560546999
1102	48-49	0.2086040586078397
1102	50-51	0.1994072834991485
1102	52-53	0.259451516881974
1102	54-55	0.18568531784871567
1102	56-57	0.18850721588476205
1102	58-59	0.2172377194263433
1102	60-61	0.21294531706304554
1102	62-63	0.1775212474594099
1102	64-65	0.14860043407229284
1102	66-67	0.1699106260947616
1102	68-69	0.1788818031450674
1102	70-71	0.19249852021473401
1102	72-73	0.2370350624212918
1102	74-75	0.2980942381198446
1102	76-77	0.1816259550235486
1102	78-79	0.2629482259092768
1102	80-81	0.22114497587397608
1102	82-83	0.15525394655848146
1102	84-85	0.1938039976424193
1102	86-87	0.17990243616414148
1102	88-89	0.18083824388806846
1102	90-91	0.250512398132372
1102	92-93	0.2686419982019572
1102	94-95	0.16520022622491837
1102	96-97	0.15093917159033765
1102	98-99	0.16644630512923442
1102	100	0.2093487763923747
1103	1	0.1025154232343084
1103	2	0.12029140785931247
1103	3	0.08432162497368267
1103	4	0.07059742349400722
1103	5	0.002692676758591972
1103	6	0.08027669834397244
1103	7	0.08181999464137135
1103	8	0.08565320655685582
1103	9	0.12844297616601352
1103	10-11	0.1147574511386793
1103	12-13	0.11640696139927798
1103	14-15	0.1651847748425368
1103	16-17	0.1670666494152684
1103	18-19	0.1436269477871548
1103	20-21	0.1471439224291089
1103	22-23	0.16038996290933483
1103	24-25	0.14391121758191616
1103	26-27	0.19168261702100864
1103	28-29	0.16845702215844938
1103	30-31	0.15321773913279202
1103	32-33	0.16085909605931903
1103	34-35	0.17129490671954528
1103	36-37	0.17066703269944128
1103	38-39	0.16729875222710433
1103	40-41	0.18147630024173367
1103	42-43	0.10712477336655724
1103	44-45	0.06264568532985493
1103	46-47	0.07203100491787495
1103	48-49	0.07591415043535932
1103	50-51	-0.04787980193373187
1103	52-53	-0.047109522258288905
1103	54-55	-0.07195480489464501
1103	56-57	-0.07948495389106114
1103	58-59	-0.025152121726669918
1103	60-61	-0.06075363330614181
1103	62-63	-0.10407057016076493
1103	64-65	-0.06514587075317735
1103	66-67	-0.05224380999940337
1103	68-69	-0.06817007528456998
1103	70-71	-0.03599091607429017
1103	72-73	-0.059003442143890084
1103	74-75	-0.09083547577925088
1103	76-77	-0.09947771941904193
1103	78-79	-0.19213710730650746
1103	80-81	-0.13696552893143377
1103	82-83	-0.07573460007754562
1103	84-85	-0.03493275259732087
1103	86-87	-0.06035134334375414
1103	88-89	-0.09976850265250192
1103	90-91	-0.10936731822644674
1103	92-93	-0.08034194459958144
1103	94-95	-0.11174041523245393
1103	96-97	-0.024114511061966937
1103	98-99	-0.011866158893326428
1103	100	-0.06744805114016117
1104	1	0.07401465475108182
1104	2	0.1252285600108607
1104	3	0.12757533752314032
1104	4	0.07374206402407424
1104	5	0.029063163159136707
1104	6	0.054274901582502366
1104	7	0.08319047796231871
1104	8	0.13359499325911628
1104	9	0.13479212463830947
1104	10-11	0.16515949899835647
1104	12-13	0.190348974267323
1104	14-15	0.20083600355957998
1104	16-17	0.21618955487193858
1104	18-19	0.19724434377891953
1104	20-21	0.18517999291449172
1104	22-23	0.22843254120414258
1104	24-25	0.18417426957085326
1104	26-27	0.12176129832176485
1104	28-29	0.21484281047245446
1104	30-31	0.1856684941828135
1104	32-33	0.15084559386374252
1104	34-35	0.17272926093185959
1104	36-37	0.18490494456352735
1104	38-39	0.13133454382396081
1104	40-41	0.1491368419137018
1104	42-43	0.0963547675806069
1104	44-45	0.14559872919973316
1104	46-47	0.1865080599930593
1104	48-49	0.15407074904985762
1104	50-51	0.06824945821468731
1104	52-53	0.12206731560659989
1104	54-55	0.12720633686805627
1104	56-57	0.137801046902986
1104	58-59	0.08591541647484036
1104	60-61	0.12332181367615647
1104	62-63	0.1727835673241387
1104	64-65	0.15799503360690892
1104	66-67	0.21620665507553838
1104	68-69	0.2291582860150143
1104	70-71	0.1767785750490134
1104	72-73	0.17443684362322642
1104	74-75	0.22694912447457938
1104	76-77	0.15483433564814675
1104	78-79	0.12745441709395777
1104	80-81	0.15294269161568508
1104	82-83	0.22189026505139964
1104	84-85	0.2551917174190166
1104	86-87	0.20564375713679794
1104	88-89	0.2026297107363817
1104	90-91	0.2538272992743771
1104	92-93	0.30473104005821483
1104	94-95	0.22058759314503362
1104	96-97	0.2653206522227052
1104	98-99	0.3600008876562981
1104	100	0.38440352103844333
1105	1	0.04615224043722321
1105	2	0.0386849547653938
1105	3	0.05374050369204042
1105	4	0.04976303716652808
1105	5	0.06159734008615203
1105	6	0.04097346823844816
1105	7	-0.04137380626197995
1105	8	-0.06592300353999292
1105	9	-0.05628876756937018
1105	10-11	-0.04164275885298707
1105	12-13	-0.008414582480931188
1105	14-15	-0.01513235502694954
1105	16-17	0.004789194073175906
1105	18-19	0.0076567743715258985
1105	20-21	0.04549923705290837
1105	22-23	0.08778752954894031
1105	24-25	-0.0215967232705907
1105	26-27	0.032370202192900877
1105	28-29	-0.015297804374661439
1105	30-31	0.03561731244247568
1105	32-33	-0.02409985315244967
1105	34-35	0.00656466361390784
1105	36-37	-0.056562810821304765
1105	38-39	-0.08847438995044854
1105	40-41	-0.040651191041888524
1105	42-43	-0.06912928686828224
1105	44-45	0.02125402856585623
1105	46-47	0.03431510693994255
1105	48-49	-0.08289180185354184
1105	50-51	-0.07615023688595812
1105	52-53	-0.0676041860358012
1105	54-55	0.006484824855121474
1105	56-57	-0.08867932681902602
1105	58-59	-0.11361635625531363
1105	60-61	-0.2079167072472572
1105	62-63	-0.20441125171446828
1105	64-65	-0.23345799281372592
1105	66-67	-0.2155290938277119
1105	68-69	-0.2405432162799528
1105	70-71	-0.14863697264489417
1105	72-73	-0.16848342930409643
1105	74-75	-0.21576048952798743
1105	76-77	-0.18451556629038635
1105	78-79	-0.23261840414011203
1105	80-81	-0.15080886849366237
1105	82-83	-0.15400486614860398
1105	84-85	-0.1621364858842682
1105	86-87	-0.16555568170777235
1105	88-89	-0.23785710929004011
1105	90-91	-0.21980259976883332
1105	92-93	-0.15836190466401945
1105	94-95	-0.19882374469559494
1105	96-97	-0.24363432643143312
1105	98-99	-0.2601306938615551
1105	100	-0.2448858778422789
1106	1	-0.06649909811748955
1106	2	0.03591220557616026
1106	3	0.04453047528892995
1106	4	0.006864171240820838
1106	5	-0.020331987287853792
1106	6	-0.01416460403572728
1106	7	-6.651182767640762E-4
1106	8	0.02888915050345986
1106	9	-0.013919069112141358
1106	10-11	-0.010616238524384869
1106	12-13	0.027822685701075045
1106	14-15	0.06554676889734168
1106	16-17	0.008080803744654474
1106	18-19	0.012832863567965092
1106	20-21	0.014086318004544296
1106	22-23	0.06381707571421202
1106	24-25	0.004411512351190083
1106	26-27	-0.029885835124275673
1106	28-29	-0.047320194773170954
1106	30-31	0.04042707888956443
1106	32-33	0.04506452662805316
1106	34-35	0.05122019110903864
1106	36-37	0.001300468252352971
1106	38-39	0.042123846784711816
1106	40-41	0.08004653416094243
1106	42-43	0.025019312463257393
1106	44-45	0.040391780077563055
1106	46-47	0.084309455087066
1106	48-49	0.030499827491830445
1106	50-51	0.010718543739812958
1106	52-53	-0.014412690046967214
1106	54-55	-0.04504268749258955
1106	56-57	-0.004248063487189313
1106	58-59	0.025806189744706387
1106	60-61	0.020433977878504095
1106	62-63	-0.027056957671099724
1106	64-65	-0.07568977586684156
1106	66-67	-0.02132681322012786
1106	68-69	-0.029551364802038904
1106	70-71	-0.04038490498983549
1106	72-73	0.07206919845071269
1106	74-75	-0.008843975097015289
1106	76-77	-0.08378204866210126
1106	78-79	-0.051992293236722276
1106	80-81	-0.04458855133976414
1106	82-83	-0.04442214958268664
1106	84-85	0.013893445377199498
1106	86-87	-0.01640945694728657
1106	88-89	0.002746940132297482
1106	90-91	0.022217555939150202
1106	92-93	0.0020451272829973277
1106	94-95	-0.08132390354777641
1106	96-97	-0.013313138088022924
1106	98-99	-0.07954813280653639
1106	100	-0.02521568554449516
1107	1	-0.03842718282213298
1107	2	-0.04649845847927736
1107	3	-0.010090596186678624
1107	4	0.0016598521382888975
1107	5	-0.1416342910781907
1107	6	-0.13523265392419148
1107	7	-0.08089941785488008
1107	8	-0.07506520543914519
1107	9	-0.05049089919954497
1107	10-11	-0.06830354567137675
1107	12-13	-0.044616853355982755
1107	14-15	0.004989198934651995
1107	16-17	-0.015815976199469617
1107	18-19	-0.056902206579337644
1107	20-21	-0.062277354544171715
1107	22-23	-0.05595379591149907
1107	24-25	-0.045623582232899196
1107	26-27	-0.08664681164331256
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2216	7	-0.10974530020117612
2216	8	-0.1357757612708852
2216	9	-0.189812532390782
2216	10-11	-0.17139103222968544
2216	12-13	-0.12467315773188403
2216	14-15	-0.23926120306380483
2216	16-17	-0.24180994663256428
2216	18-19	-0.1687024072916472
2216	20-21	-0.18441313265232395
2216	22-23	-0.21082908485215768
2216	24-25	-0.273518927467002
2216	26-27	-0.31240651448415946
2216	28-29	-0.2782720957443203
2216	30-31	-0.26959078353924326
2216	32-33	-0.325589232353515
2216	34-35	-0.2916643463137376
2216	36-37	-0.3873731498623272
2216	38-39	-0.36263020713849414
2216	40-41	-0.4160954990332044
2216	42-43	-0.3577303430193268
2216	44-45	-0.3565754319164185
2216	46-47	-0.36114255471796497
2216	48-49	-0.35449676706115696
2216	50-51	-0.32587580229426294
2216	52-53	-0.3438638199421433
2216	54-55	-0.268695189248902
2216	56-57	-0.2509871585732597
2216	58-59	-0.35805564075605645
2216	60-61	-0.33974747719897636
2216	62-63	-0.29334051000873984
2216	64-65	-0.34155526939427716
2216	66-67	-0.32415946885164715
2216	68-69	-0.29362461018380515
2216	70-71	-0.2276416686732503
2216	72-73	-0.29230434712037834
2216	74-75	-0.2788256156170661
2216	76-77	-0.27709775484981947
2216	78-79	-0.2611280839120411
2216	80-81	-0.28413726586205
2216	82-83	-0.2840308705573946
2216	84-85	-0.27002359660311726
2216	86-87	-0.2877256520559399
2216	88-89	-0.2165899936497766
2216	90-91	-0.24233939041517516
2216	92-93	-0.2617948366632419
2216	94-95	-0.31090471020448973
2216	96-97	-0.2781100178633622
2216	98-99	-0.19232912837964022
2216	100	-0.2407849162682396
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	16.0
3	6.0
4	14.0
5	36.0
6	52.0
7	112.0
8	258.0
9	572.0
10	1052.0
11	1687.0
12	2658.0
13	3922.0
14	4704.0
15	6147.0
16	7649.0
17	11062.0
18	12818.0
19	7075.0
20	5044.0
21	4835.0
22	5014.0
23	5338.0
24	6334.0
25	7738.0
26	9613.0
27	12577.0
28	16207.0
29	20979.0
30	26840.0
31	34490.0
32	44544.0
33	57880.0
34	80625.0
35	124218.0
36	232383.0
37	540899.0
38	1004646.0
39	519218.0
40	2164.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.14179863508956	19.78875355515641	13.166557459167807	23.902890350586226
2	15.573089804029843	20.077309164708716	40.48647497216806	23.86312605909338
3	18.117327904400117	25.32892941370782	30.199303472782912	26.354439209109152
4	11.973770626603583	16.586700937862076	39.27246486428628	32.167063571248065
5	12.139935058718747	38.14094768319604	36.38796565302578	13.331151605059436
6	30.824421160677133	38.72352310288925	17.47959007904525	12.972465657388366
7	27.180584683972285	32.660397494930564	22.598472558402648	17.560545262694504
8	25.1207195252313	36.682679902573746	20.17114810435611	18.025452467838843
9	25.137333279932346	15.939273153492515	21.237383573738196	37.686009992836944
10-11	23.747060206857235	26.632492319012325	29.30172082124055	20.31872665288989
12-13	24.71877695888533	23.931001557368507	29.507100310268637	21.843121173477524
14-15	21.640632531657772	25.994426927679072	26.771073755663803	25.593866784999346
16-17	20.893778536101955	29.537404135355665	27.27845777277164	22.290359555770735
18-19	20.713334826669087	28.159787398136544	29.934086475481887	21.192791299712486
20-21	21.885847794696726	27.43931614722484	29.631842196109343	21.04299386196909
22-23	21.457791791219876	27.279368034087824	29.70995435628468	21.552885818407624
24-25	21.36095485133657	27.416072064332063	29.60895448698148	21.614018597349887
26-27	21.153626335183667	27.362233714228296	29.76050916900107	21.723630781586962
28-29	21.153718343283355	27.45051055718657	29.80402721199355	21.59174388753652
30-31	21.371581355374527	27.892764755270143	29.433228998842726	21.302424890512604
32-33	21.052212683821605	27.57879373000388	29.911367855572202	21.457625730602313
34-35	21.22817137298058	27.31967717598475	29.92103121029225	21.53112024074242
36-37	21.522299661820696	27.746621131757337	29.307124171258625	21.423955035163345
38-39	21.396305131698504	27.912542280826578	29.38530298792189	21.305849599553024
40-41	21.23686431063335	27.632704143857957	29.583664236889128	21.546767308619565
42-43	21.264296098925584	27.54222491808137	29.471225583498505	21.722253399494537
44-45	21.37200074801276	27.80345698882324	29.312936100744203	21.511606162419803
46-47	21.545690360327864	28.062662787389808	28.968751024690953	21.422895827591372
48-49	21.49845406458279	27.995884594968466	28.756578754974775	21.749082585473968
50-51	21.522044890149502	27.9349233907014	28.738340945938567	21.80469077321053
52-53	21.54963517323135	27.921392152932516	28.724753617208986	21.804219056627147
54-55	21.66055896867511	28.341284151330076	28.522051690300543	21.476105189694266
56-57	21.47380311602933	28.04943879643305	28.561268784024087	21.915489303513535
58-59	21.345242574195137	27.637148934684603	29.00406164398492	22.01354684713534
60-61	21.64010815154046	27.99088328661027	28.641213803799975	21.727794758049296
62-63	21.414649689655857	28.022488253991657	28.685655692383595	21.87720636396889
64-65	21.423834569330378	27.783935216576243	28.785463214452427	22.006766999640952
66-67	21.315639119703185	27.834679940398637	28.718705385813912	22.130975554084266
68-69	21.769362946118125	27.952033640108397	28.566747501412422	21.711855912361052
70-71	21.720730802867706	27.804569866033653	28.558942446483016	21.91575688461562
72-73	21.546041759594445	27.91749803245645	28.62531750508566	21.911142702863444
74-75	21.583688740358113	27.95631125255324	28.495349670537422	21.964650336551227
76-77	21.621852002354135	27.93589673839364	28.358184621093695	22.08406663815853
78-79	21.69552235208762	28.107033129175306	28.411205612198515	21.786238906538557
80-81	21.62169490275902	27.88257041626402	28.510587847921176	21.985146833055783
82-83	21.494718441522906	27.948824368997037	28.603990684126902	21.952466505353154
84-85	21.476532308270848	27.89678890587599	28.466111851774006	22.16056693407915
86-87	21.628847702436786	27.994835232138097	28.352562394025142	22.023754671399974
88-89	21.373943530682713	27.96514953785781	28.42280818281252	22.238098748646962
90-91	21.38906000015595	27.883648197755317	28.44476516484926	22.282526637239467
92-93	21.492979082208784	28.082607872756544	28.28240045990928	22.142012585125396
94-95	21.295773839186282	27.802767111382682	28.468423414259313	22.43303563517172
96-97	21.22049275791745	27.796103814170564	28.50840319753203	22.47500023037996
98-99	21.270839639246255	27.871774769212447	28.404466393944055	22.452919197597243
100	21.398718236806495	27.798283562992616	28.257732082996327	22.545266117204562
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	39.0
1	38.0
2	109.5
3	193.5
4	140.0
5	98.5
6	261.0
7	600.5
8	1056.5
9	1326.5
10	1116.0
11	868.5
12	907.5
13	1066.0
14	1264.5
15	1437.0
16	1628.5
17	1838.5
18	2132.0
19	2557.0
20	3226.5
21	4217.5
22	5655.0
23	8057.0
24	10878.5
25	14105.5
26	18776.0
27	24931.0
28	32814.0
29	41138.5
30	49052.0
31	56941.5
32	64473.0
33	72219.5
34	80162.5
35	87036.0
36	92000.5
37	96558.5
38	100190.0
39	100863.5
40	101595.0
41	102233.5
42	101009.0
43	99982.5
44	98637.0
45	96820.0
46	95314.5
47	93036.0
48	90715.0
49	88598.0
50	85412.0
51	81781.5
52	76546.5
53	72090.0
54	69111.0
55	65546.5
56	61131.5
57	55828.0
58	50688.0
59	45809.0
60	40867.0
61	34963.0
62	29187.5
63	24091.5
64	19446.0
65	15572.5
66	12172.5
67	8963.5
68	6572.0
69	4928.0
70	3382.0
71	2284.5
72	1678.5
73	1179.5
74	823.0
75	571.5
76	348.0
77	233.5
78	163.5
79	85.0
80	36.0
81	13.0
82	8.5
83	5.5
84	2.0
85	2.5
86	2.5
87	1.0
88	1.0
89	0.5
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.09332160403994291
2	9.569628974851723E-4
3	0.0
4	3.1898763249505743E-4
5	0.03657724852609992
6	1.4177228110891444E-4
7	0.003792408519663461
8	3.544307027722861E-4
9	1.7721535138614305E-4
10-11	4.0759530818812895E-4
12-13	0.0
14-15	7.088614055445722E-5
16-17	0.0
18-19	7.088614055445722E-5
20-21	0.0
22-23	2.835445622178289E-4
24-25	8.860767569307152E-5
26-27	0.0015772166273366728
28-29	0.008630387612505166
30-31	0.03579750098000089
32-33	0.02560761827529767
34-35	0.028992431486773003
36-37	0.02597977051320857
38-39	0.024916478404891713
40-41	0.030693698860079974
42-43	0.02264812190714908
44-45	0.021921538966465894
46-47	0.016073432370723173
48-49	0.011288617883297311
50-51	0.008081020023208122
52-53	0.0055113974281090486
54-55	0.004359497644099119
56-57	0.00280000255190106
58-59	0.0011164567137327012
60-61	5.139245190198148E-4
62-63	0.0014886089516436014
64-65	0.0030835471141188887
66-67	4.962029838812005E-4
68-69	0.0011341782488713155
70-71	3.012660973564432E-4
72-73	5.139245190198148E-4
74-75	4.607599136039719E-4
76-77	3.367091676336718E-4
78-79	1.7721535138614305E-5
80-81	2.658230270792145E-4
82-83	1.7721535138614305E-4
84-85	1.5949381624752871E-4
86-87	7.088614055445722E-5
88-89	0.0
90-91	0.0
92-93	0.0
94-95	0.0
96-97	0.0
98-99	0.0
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2821426.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.27992828900594
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.44279294560452	50.554599699893984
2	14.046940066116129	18.339664803920506
3	4.117992175842104	8.064667018009802
4	1.6658846167615236	4.349953132798016
5	0.8363714933657559	2.7299135554942677
6	0.4759087017223633	1.864037155232988
7	0.3074425604614479	1.4048879809938186
8	0.21599397981690444	1.1280057210643615
9	0.14506486938508703	0.8522841843641246
>10	0.70342346765315	7.84520154259774
>50	0.03232854745679575	1.435630830450879
>100	0.009530560000830426	0.9955413917706633
>500	1.0867193791279683E-4	0.03855641346182313
>1k	2.1734387541719278E-4	0.3970565699470722
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4404	0.15609128150091478	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3691	0.13082037239325078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	8.860767569307152E-5	0.0
22-23	0.0	0.0	0.0	1.0632921083168583E-4	0.0
24-25	0.0	0.0	0.0	1.7721535138614305E-4	0.0
26-27	0.0	1.7721535138614305E-5	0.0	2.4810149194060026E-4	0.0
28-29	0.0	3.544307027722861E-5	0.0	7.797475460990294E-4	0.0
30-31	0.0	3.544307027722861E-5	0.0	0.003331648606059489	0.0
32-33	0.0	3.544307027722861E-5	0.0	0.009853173537069552	0.0
34-35	0.0	3.544307027722861E-5	0.0	0.01828862426304996	0.0
36-37	0.0	3.544307027722861E-5	0.0	0.035248133390703845	0.0
38-39	0.0	3.544307027722861E-5	0.0	0.06549879387231847	0.0
40-41	0.0	3.544307027722861E-5	0.0	0.10117224410634906	0.0
42-43	0.0	3.544307027722861E-5	0.0	0.1369165804809341	0.0
44-45	0.0	3.544307027722861E-5	0.0	0.17684319914823213	0.0
46-47	0.0	3.544307027722861E-5	0.0	0.22614450990385715	0.0
48-49	0.0	3.544307027722861E-5	0.0	0.28455468972072984	0.0
50-51	0.0	3.544307027722861E-5	0.0	0.3487952545982067	0.0
52-53	0.0	3.544307027722861E-5	0.0	0.41440037768135685	0.0
54-55	0.0	3.544307027722861E-5	0.0	0.48585360736024974	0.0
56-57	0.0	3.544307027722861E-5	0.0	0.5699245700578359	0.0
58-59	0.0	3.544307027722861E-5	0.0	0.6658689612982938	0.0
60-61	0.0	5.3164605415842914E-5	0.0	0.7675196868533856	0.0
62-63	0.0	7.088614055445722E-5	0.0	0.8699678814897147	0.0
64-65	0.0	7.088614055445722E-5	0.0	0.9808515268520244	0.0
66-67	0.0	7.088614055445722E-5	0.0	1.1051326527791265	0.0
68-69	0.0	7.088614055445722E-5	0.0	1.2480391121369123	0.0
70-71	0.0	7.088614055445722E-5	0.0	1.4069126746545897	0.0
72-73	1.7721535138614305E-5	7.088614055445722E-5	0.0	1.5728039650871581	0.0
74-75	3.544307027722861E-5	7.088614055445722E-5	0.0	1.7340167702431324	0.0
76-77	3.544307027722861E-5	7.088614055445722E-5	0.0	1.9062169271850475	0.0
78-79	3.544307027722861E-5	7.088614055445722E-5	0.0	2.1129386345769836	0.0
80-81	3.544307027722861E-5	7.088614055445722E-5	0.0	2.3389413721997316	0.0
82-83	3.544307027722861E-5	7.088614055445722E-5	0.0	2.58144285903653	0.0
84-85	3.544307027722861E-5	7.088614055445722E-5	0.0	2.8125493987791987	0.0
86-87	3.544307027722861E-5	7.088614055445722E-5	0.0	3.0541116442536502	0.0
88	3.544307027722861E-5	7.088614055445722E-5	0.0	3.2596637303264377	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3990	0.0	44.718475	1
GTATCAA	6795	0.0	38.383183	1
TCAACGC	7540	0.0	33.671707	4
ATCAACG	7650	0.0	33.31046	3
CAACGCA	7765	0.0	32.69603	5
AACGCAG	8055	0.0	31.621614	6
TATCAAC	8360	0.0	30.7632	2
GTACATG	7080	0.0	29.324186	1
TACATGG	7165	0.0	27.166557	2
ACGCAGA	9310	0.0	27.10659	7
CGCAGAG	9515	0.0	26.421928	8
ACATGGG	7255	0.0	25.662592	3
GCAGAGT	10605	0.0	23.30744	9
TATACCG	175	4.4291883E-6	21.492884	5
CCTATAC	1195	0.0	21.24558	3
CATGGGG	4765	0.0	20.621778	4
GAGTACT	7385	0.0	19.789427	12-13
AGAGTAC	9605	0.0	19.667454	10-11
CAGAGTA	10550	0.0	19.531548	10-11
GAGTACA	5290	0.0	19.311888	1
>>END_MODULE