FastQCFastQC Report
Wed 25 May 2016
SRR1033156_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033156_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851712
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG49100.028.4943491
ACACCGT2850.026.364516
ACATGGG49650.025.2707563
TACATGG51150.025.0978622
ACCGTTC1551.5586138E-624.238348
GTATCAA28850.024.0024361
GGTATCA22150.022.543221
TCAACGC27550.022.5104734
ATCAACG28400.022.0069313
CAACGCA28450.021.6326435
AACGCAG29150.020.9435066
GAGTACA33850.020.1786981
CACCGTT2109.608993E-720.1264787
CGTATAC1656.870423E-519.9361043
CGACGCG600.004923469419.6803690-91
CGCGCAC1200.00492603319.67795494
TATCAAC32900.019.5814292
CATGGGG32250.019.3755594
TACACCG2251.8538085E-618.7923265
ACGCAGA34450.017.7214287
Produced by FastQC (version 0.11.5)