##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033156_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1851712
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51989402239657	33.0	31.0	34.0	30.0	34.0
2	31.60319099298379	33.0	31.0	34.0	30.0	34.0
3	31.686676977845366	34.0	31.0	34.0	30.0	34.0
4	35.050164388414615	37.0	35.0	37.0	33.0	37.0
5	35.12451180313137	37.0	35.0	37.0	33.0	37.0
6	35.219547100197005	37.0	35.0	37.0	33.0	37.0
7	35.12098047644558	37.0	35.0	37.0	33.0	37.0
8	35.143078405281166	37.0	35.0	37.0	33.0	37.0
9	36.7791562618809	39.0	38.0	39.0	33.0	39.0
10-11	36.776440126758374	39.0	38.0	39.0	33.0	39.0
12-13	36.74955230619017	39.0	38.0	39.0	33.0	39.0
14-15	38.19430856418277	41.0	39.0	41.0	34.0	41.0
16-17	38.21488249792624	41.0	39.0	41.0	34.0	41.0
18-19	38.20450453418242	41.0	39.0	41.0	33.5	41.0
20-21	38.22602947974631	41.0	39.0	41.0	34.0	41.0
22-23	38.19840936387516	41.0	39.0	41.0	34.0	41.0
24-25	38.19327438608164	40.5	39.0	41.0	33.5	41.0
26-27	38.149535942954415	40.0	38.5	41.0	33.0	41.0
28-29	38.124883351190675	40.0	38.5	41.0	33.0	41.0
30-31	38.13151019164967	40.0	38.0	41.0	33.0	41.0
32-33	38.19675089862787	41.0	39.0	41.0	33.0	41.0
34-35	38.169699445702136	41.0	39.0	41.0	33.0	41.0
36-37	38.092808708913694	41.0	38.0	41.0	33.0	41.0
38-39	38.014169860107835	41.0	38.0	41.0	33.0	41.0
40-41	37.95325974017558	40.5	38.0	41.0	33.0	41.0
42-43	37.84942582863858	40.0	38.0	41.0	33.0	41.0
44-45	37.739867484792455	40.0	38.0	41.0	32.5	41.0
46-47	37.61545234896139	40.0	38.0	41.0	32.0	41.0
48-49	37.50041178109771	40.0	38.0	41.0	32.0	41.0
50-51	37.382351305170566	40.0	37.5	41.0	31.5	41.0
52-53	37.241652319583174	40.0	37.0	41.0	31.0	41.0
54-55	37.09174455854907	40.0	37.0	41.0	31.0	41.0
56-57	36.93750810061176	40.0	36.5	41.0	31.0	41.0
58-59	36.74509588964159	40.0	36.0	41.0	30.5	41.0
60-61	36.52469228476134	39.5	35.5	41.0	30.0	41.0
62-63	36.26483168008848	39.0	35.0	41.0	30.0	41.0
64-65	35.99697253136557	39.0	35.0	41.0	30.0	41.0
66-67	35.705791721390796	38.0	35.0	40.5	29.5	41.0
68-69	35.39366974993952	37.5	35.0	40.0	29.0	41.0
70-71	35.04554649967166	37.0	35.0	40.0	29.0	41.0
72-73	34.67772526181177	36.5	34.0	39.0	29.0	41.0
74-75	34.31195023848201	36.0	34.0	39.0	28.5	41.0
76-77	33.98548856409636	35.5	34.0	38.5	28.0	40.0
78-79	33.61178628210003	35.0	34.0	37.0	28.0	39.5
80-81	33.24372364600975	35.0	34.0	37.0	27.5	39.0
82-83	33.0115714538762	35.0	34.0	36.5	27.5	39.0
84-85	32.73383819946082	35.0	34.0	36.0	27.0	37.5
86-87	32.46936942677911	35.0	34.0	36.0	27.0	37.0
88-89	32.09476203642899	35.0	33.5	35.5	26.0	37.0
90-91	31.893431321933434	35.0	33.0	35.0	26.0	36.0
92-93	31.701791099263815	35.0	33.0	35.0	25.0	36.0
94-95	31.519262444699827	35.0	33.0	35.0	25.0	36.0
96-97	31.338207291414648	35.0	33.0	35.0	24.5	36.0
98-99	31.140818604621018	35.0	33.0	35.0	24.0	35.5
100	30.97319669581447	35.0	33.0	35.0	22.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2022470344571552
1101	2	0.28458013324738474
1101	3	0.16806354917078892
1101	4	0.21477454410502617
1101	5	0.17023310237814115
1101	6	0.06402586388811216
1101	7	0.16734838232184046
1101	8	0.09578358779541674
1101	9	0.14455451905900674
1101	10-11	0.11395308434742901
1101	12-13	0.20397762554862453
1101	14-15	0.20817470175867925
1101	16-17	0.09442146510116345
1101	18-19	0.10450299405043495
1101	20-21	0.14352524094213237
1101	22-23	0.19646824533999308
1101	24-25	0.1068642327357665
1101	26-27	0.11741545048447222
1101	28-29	0.14232280036546285
1101	30-31	0.13352904906213325
1101	32-33	0.12695804652958742
1101	34-35	0.13148685670692117
1101	36-37	0.10195650689468749
1101	38-39	0.10205591542815284
1101	40-41	-0.0033573423757005116
1101	42-43	0.05958725548145338
1101	44-45	-0.08948289029238765
1101	46-47	-0.09616117301546723
1101	48-49	-0.033940216119916045
1101	50-51	-0.05128076821173977
1101	52-53	-0.05118420293326409
1101	54-55	-0.14457577401980615
1101	56-57	-0.08585402957595534
1101	58-59	-0.16797967886311227
1101	60-61	-0.2230400484578965
1101	62-63	-0.21496984562271138
1101	64-65	-0.16475086285353768
1101	66-67	-0.1479657663654379
1101	68-69	-0.08593302162395844
1101	70-71	-0.0875110021017278
1101	72-73	-0.0703387723893627
1101	74-75	-0.11082632789177893
1101	76-77	-0.14080690272056273
1101	78-79	-0.1661344971942782
1101	80-81	-0.17707795981654328
1101	82-83	-0.14148406005091374
1101	84-85	-0.14600642440380085
1101	86-87	-0.07001686775102911
1101	88-89	0.10665874782359452
1101	90-91	0.15620227042000678
1101	92-93	0.12229090296569822
1101	94-95	0.21438866122577238
1101	96-97	0.14934667673911406
1101	98-99	0.1406386211449977
1101	100	0.16713441332716172
1102	1	0.23239508437237077
1102	2	0.2583815304095829
1102	3	0.2708614969076457
1102	4	0.23998397350050737
1102	5	0.18534245495868618
1102	6	0.1312860346201674
1102	7	0.18380743126599697
1102	8	0.2830290477429571
1102	9	0.24802640914118257
1102	10-11	0.2639777230998348
1102	12-13	0.3247111357012358
1102	14-15	0.3637385306208074
1102	16-17	0.28911471263366195
1102	18-19	0.2986853498050337
1102	20-21	0.29689060465360484
1102	22-23	0.3330312480792301
1102	24-25	0.4060085795914361
1102	26-27	0.38401218657445213
1102	28-29	0.31111818277339864
1102	30-31	0.3384229212594718
1102	32-33	0.4109260089047595
1102	34-35	0.34731870446842095
1102	36-37	0.325078526692657
1102	38-39	0.2559575343391174
1102	40-41	0.27321899427661833
1102	42-43	0.25364551163752225
1102	44-45	0.2746679061190491
1102	46-47	0.2844568248684993
1102	48-49	0.26320132626598536
1102	50-51	0.22160428476157534
1102	52-53	0.1843966918882458
1102	54-55	0.18162319115259606
1102	56-57	0.09534316015692923
1102	58-59	0.1227893038462895
1102	60-61	0.029715506131438474
1102	62-63	0.0871166581383207
1102	64-65	0.11762101563392946
1102	66-67	0.13242685940090126
1102	68-69	0.15940010880200361
1102	70-71	0.2342086016491507
1102	72-73	0.22187411367814747
1102	74-75	0.17838823940825677
1102	76-77	0.1770230391609715
1102	78-79	0.08558478045547702
1102	80-81	0.04271329922370626
1102	82-83	0.10967631648719589
1102	84-85	0.1613801286347396
1102	86-87	0.21322823710860206
1102	88-89	0.27441604161897715
1102	90-91	0.3575887437449232
1102	92-93	0.3323145271812429
1102	94-95	0.31528797568872236
1102	96-97	0.3018324732230191
1102	98-99	0.34934878245636014
1102	100	0.312557402732498
1103	1	0.04998362478684726
1103	2	0.05973209621108211
1103	3	-0.04502914538710456
1103	4	-0.04383534052252003
1103	5	-0.04086896885410596
1103	6	0.03929494528242117
1103	7	-0.07845242394735408
1103	8	0.023451857040612367
1103	9	-0.0432815139381546
1103	10-11	0.08318024670344926
1103	12-13	-0.0017526426657852312
1103	14-15	0.08385269267360229
1103	16-17	0.055915556080343265
1103	18-19	0.01255910109860281
1103	20-21	-0.019343101622077086
1103	22-23	0.03513738217355211
1103	24-25	0.04784782355565653
1103	26-27	0.06858975474879969
1103	28-29	0.04510407809634387
1103	30-31	0.08584326305165746
1103	32-33	0.08129168345885063
1103	34-35	0.04754190656925772
1103	36-37	0.08827669020423201
1103	38-39	-0.009148169437111164
1103	40-41	0.10163060078586028
1103	42-43	0.1001497869435326
1103	44-45	0.09593600009448267
1103	46-47	0.10441966420131621
1103	48-49	0.0402307899885983
1103	50-51	0.03765724001894455
1103	52-53	0.08056503162698903
1103	54-55	0.03085550020112038
1103	56-57	-0.01755109483528372
1103	58-59	0.039260919112884096
1103	60-61	0.03897576553020343
1103	62-63	0.030832460626385227
1103	64-65	0.0718440417747388
1103	66-67	0.09396231679824751
1103	68-69	0.07376593840997714
1103	70-71	0.09545504615322642
1103	72-73	0.05499047319874251
1103	74-75	0.08570223862543003
1103	76-77	0.05530406939240606
1103	78-79	-0.028152471153426006
1103	80-81	-0.04226719128083545
1103	82-83	-0.05410595591751388
1103	84-85	-0.0474770196978227
1103	86-87	-2.885559039356167E-5
1103	88-89	0.025261288549550898
1103	90-91	0.12702601138186864
1103	92-93	0.2066193979638662
1103	94-95	0.1504280932973856
1103	96-97	0.09476330815625289
1103	98-99	0.2294815306500091
1103	100	0.2931814742965422
1104	1	0.1539391623580464
1104	2	0.1293659719174478
1104	3	0.12406100512680496
1104	4	0.1322756595750363
1104	5	-0.019321642394594107
1104	6	-0.011141218963238941
1104	7	-9.183531808787393E-5
1104	8	-0.003126073689884379
1104	9	-0.009801428460725958
1104	10-11	-0.010234375214928093
1104	12-13	-0.01902495533111903
1104	14-15	0.016374535165979864
1104	16-17	0.041540982885408084
1104	18-19	0.07550485841522203
1104	20-21	0.003311329144771946
1104	22-23	0.0847568945191739
1104	24-25	0.05563133388617558
1104	26-27	0.08106078271035244
1104	28-29	0.15296826092238547
1104	30-31	0.12382839605915308
1104	32-33	0.1258040326496328
1104	34-35	0.12202548761676013
1104	36-37	0.17146113126796791
1104	38-39	0.10394322830099867
1104	40-41	0.12584898957272372
1104	42-43	0.06694037140062647
1104	44-45	0.13851880831147412
1104	46-47	0.015821395946126415
1104	48-49	-0.016636320500580837
1104	50-51	-0.07874747195182152
1104	52-53	-0.07554660265235924
1104	54-55	-0.10573090485507919
1104	56-57	-0.022708178381734
1104	58-59	-0.019092876343776766
1104	60-61	0.04783238573828186
1104	62-63	0.0733246408362831
1104	64-65	-0.02872597910898378
1104	66-67	0.09665673244185768
1104	68-69	0.07272632304409399
1104	70-71	0.039685255860284485
1104	72-73	0.14873961173275774
1104	74-75	0.08451579017634003
1104	76-77	-0.003515863969937527
1104	78-79	0.008759837643978585
1104	80-81	-0.02781091718746609
1104	82-83	0.009201456425067533
1104	84-85	0.1474629665644258
1104	86-87	0.1563015999763948
1104	88-89	0.21029304120379066
1104	90-91	0.1922771052383503
1104	92-93	0.15521175004560206
1104	94-95	0.1498615432735093
1104	96-97	0.19676945086401076
1104	98-99	0.19486234610782205
1104	100	0.22354288507680664
1105	1	0.3334283951440895
1105	2	0.30514466473160695
1105	3	0.3814770715698401
1105	4	0.40762151558015347
1105	5	0.3309620393335422
1105	6	0.38417716031069915
1105	7	0.29239497368071454
1105	8	0.28719819953794
1105	9	0.3224328264684999
1105	10-11	0.380865314882044
1105	12-13	0.3516166985911795
1105	14-15	0.4074456551765522
1105	16-17	0.4504791206816776
1105	18-19	0.4142228746842349
1105	20-21	0.42175657526095733
1105	22-23	0.4684831672684524
1105	24-25	0.4892370628478133
1105	26-27	0.39378990999585994
1105	28-29	0.42258540253469334
1105	30-31	0.44660293055217437
1105	32-33	0.41588635295821064
1105	34-35	0.41403546462058216
1105	36-37	0.40056499754237507
1105	38-39	0.3331855496095315
1105	40-41	0.40057358355470996
1105	42-43	0.3460633851271737
1105	44-45	0.35234729329199155
1105	46-47	0.4141109955281195
1105	48-49	0.40988560662867
1105	50-51	0.4772100641089878
1105	52-53	0.5194860894931423
1105	54-55	0.42315839732331284
1105	56-57	0.4719368534038608
1105	58-59	0.4675130413452493
1105	60-61	0.40552945546385644
1105	62-63	0.41857483650017
1105	64-65	0.443927159988057
1105	66-67	0.4173211325483308
1105	68-69	0.35845218592251626
1105	70-71	0.4021338880811385
1105	72-73	0.3809132513777129
1105	74-75	0.34482244884704727
1105	76-77	0.38906493786602425
1105	78-79	0.3197324540402917
1105	80-81	0.3417950024245613
1105	82-83	0.3291554503445795
1105	84-85	0.349229861581172
1105	86-87	0.3766102063577961
1105	88-89	0.3429614652970585
1105	90-91	0.3607631461413767
1105	92-93	0.3603074035190268
1105	94-95	0.368390700282891
1105	96-97	0.3940638224745747
1105	98-99	0.26779552699410303
1105	100	0.29685139173761854
1106	1	0.07473275936417778
1106	2	0.08365985162045675
1106	3	-0.0025628283552983078
1106	4	0.0420031767386817
1106	5	-0.00609332975688659
1106	6	0.011481626987645654
1106	7	0.02012497437502958
1106	8	-0.03159163414129296
1106	9	0.05204592700806643
1106	10-11	0.0232143619942633
1106	12-13	0.0073641252727654205
1106	14-15	0.06244214329871056
1106	16-17	0.06204872334409828
1106	18-19	0.041937298815582835
1106	20-21	0.043311430896899594
1106	22-23	0.026266689969268953
1106	24-25	0.09677788761511152
1106	26-27	0.16990204148596888
1106	28-29	0.10789280301436577
1106	30-31	0.12162706902392273
1106	32-33	0.08249752215998996
1106	34-35	0.12171454113583735
1106	36-37	0.1434699901298515
1106	38-39	0.11639561399352516
1106	40-41	-0.01967155358014594
1106	42-43	0.08313750330258074
1106	44-45	0.0894331784035245
1106	46-47	0.0740971846556846
1106	48-49	0.11438943068787921
1106	50-51	0.11701010680801005
1106	52-53	0.13602211933149277
1106	54-55	0.12664304868784626
1106	56-57	0.16795931694001354
1106	58-59	0.15696441867488176
1106	60-61	0.20586730305330292
1106	62-63	0.23508884071442537
1106	64-65	0.20250701171686103
1106	66-67	0.25592720179096773
1106	68-69	0.14976446844548263
1106	70-71	0.27357250459109395
1106	72-73	0.27353384817070747
1106	74-75	0.2885770792339244
1106	76-77	0.30984595817510296
1106	78-79	0.23193693816611471
1106	80-81	0.19562618680762967
1106	82-83	0.19514584540418412
1106	84-85	0.25088805041956874
1106	86-87	0.16150073224492445
1106	88-89	0.2370106071428495
1106	90-91	0.20169627327811313
1106	92-93	0.20777014003769168
1106	94-95	0.22469300626432087
1106	96-97	0.115893777375355
1106	98-99	0.18407494660980817
1106	100	0.16376578383169615
1107	1	0.12098915162902202
1107	2	0.010513792595212124
1107	3	0.057994959834491766
1107	4	0.049415930957486864
1107	5	0.06730634834585203
1107	6	-0.027931709522427184
1107	7	-0.10329533703746563
1107	8	-0.07058802290281818
1107	9	-0.02702932796788815
1107	10-11	-0.1369438187384162
1107	12-13	-0.12332557128220856
1107	14-15	-0.12259526299666135
1107	16-17	-0.04989198771866654
1107	18-19	-0.09293031240375171
1107	20-21	-0.16507794566232548
1107	22-23	-0.06919954790401306
1107	24-25	-0.0458082311100938
1107	26-27	-0.0020868269787825966
1107	28-29	-0.014754058361404532
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2216	24-25	-0.23227164956004742
2216	26-27	-0.2831846049380573
2216	28-29	-0.2643731999755943
2216	30-31	-0.2528174104095555
2216	32-33	-0.24834097457597437
2216	34-35	-0.2442422810938467
2216	36-37	-0.2773723121081346
2216	38-39	-0.15872216709456666
2216	40-41	-0.1858624168526788
2216	42-43	-0.303809304532372
2216	44-45	-0.17523414973375395
2216	46-47	-0.009743895872048824
2216	48-49	-0.007193636802938386
2216	50-51	-0.019177388621912428
2216	52-53	-0.04993648555972641
2216	54-55	-0.07962359371185102
2216	56-57	-0.09393565227171763
2216	58-59	-0.07734898709998816
2216	60-61	-0.05897226007720491
2216	62-63	0.013933238380310797
2216	64-65	0.1076091064509086
2216	66-67	0.08015127700861058
2216	68-69	0.06727805056506497
2216	70-71	-0.02369954290242049
2216	72-73	0.05125191331044476
2216	74-75	0.02088104117250822
2216	76-77	-0.1023749338708626
2216	78-79	-0.07146719814238622
2216	80-81	-0.03541283266447692
2216	82-83	-0.02216321099676577
2216	84-85	0.011391476171979775
2216	86-87	-0.09645092560361945
2216	88-89	-0.07234452305281991
2216	90-91	-0.07819984132033753
2216	92-93	-0.0705048200258851
2216	94-95	-0.0850064871715901
2216	96-97	-0.125978220054197
2216	98-99	-0.2184690288214881
2216	100	-0.26380900122148887
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	23209.0
3	4001.0
4	2493.0
5	2028.0
6	1740.0
7	1784.0
8	2104.0
9	2278.0
10	2526.0
11	2795.0
12	3091.0
13	3490.0
14	3918.0
15	4071.0
16	3972.0
17	4001.0
18	4019.0
19	4013.0
20	4024.0
21	4281.0
22	4902.0
23	6840.0
24	10286.0
25	14107.0
26	14832.0
27	12720.0
28	13065.0
29	16463.0
30	20246.0
31	24263.0
32	31748.0
33	43733.0
34	60692.0
35	92222.0
36	169657.0
37	364761.0
38	599382.0
39	267147.0
40	808.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.388559839310865	19.80519638850403	12.901783803319441	23.904459968865666
2	15.328974687267504	19.99546905548731	40.722593733238746	23.952962524006434
3	18.100926607918993	25.532487477796696	30.039832197882426	26.326753716401885
4	11.905185105147286	16.600980564297192	39.45774524237226	32.03608908818325
5	12.408072146211481	38.47797945656942	36.01805168058056	13.095896716638537
6	30.77578240653977	38.70713553900138	17.7751985754738	12.741883478985052
7	27.136663278609554	32.80235169329046	22.711576245867093	17.3494087822329
8	24.873733622909267	37.27568172501125	20.247666279761244	17.60291837231824
9	24.683764118469018	17.027612271232325	21.15478827495278	37.13383533534588
10-11	23.59789017114677	27.3234449734921	29.142564689804924	19.936100165556205
12-13	24.128396934154427	24.62282543147042	29.14727287899816	22.10150475537699
14-15	21.416698062123846	26.689217382145504	26.066196477497222	25.82788807823343
16-17	21.226402227759873	30.101495300903412	26.67464815853587	21.997454312800844
18-19	21.14947309289877	29.20010586754674	28.466297930721574	21.18412310883292
20-21	22.322285121645564	28.530827319476256	27.91852123935871	21.228366319519466
22-23	21.783696444452005	28.318022199591077	28.075209952520495	21.823071403436423
24-25	21.768635460324784	28.412186451563766	28.033635306376333	21.785542781735124
26-27	21.557896158260753	28.35082033623658	28.08291134967254	22.008372155830127
28-29	21.59955190206662	28.338116249437583	28.25342271525958	21.808909133236217
30-31	21.818800528801162	28.86074137491003	27.770649374850613	21.549808721438186
32-33	21.443298802009004	28.47329881281242	28.343090620148594	21.740311765029986
34-35	21.475588605909635	28.21778702217882	28.460191493050583	21.84643287886096
36-37	21.947640427470493	28.590208274936096	27.788579579302237	21.673571718291175
38-39	21.812519511438968	28.701799319246568	27.975163626032824	21.510517543281637
40-41	21.482159995376822	28.4494915316485	28.31743936537187	21.750909107602805
42-43	21.40345940626839	28.385866948446996	28.261323354003288	21.949350291281327
44-45	21.55548426401203	28.506895836573857	28.191592782703374	21.746027116710735
46-47	21.63614617968908	28.696746896756025	28.044543807540677	21.622563116014216
48-49	21.58625566962539	28.466197731906817	28.18091063425546	21.766635964212334
50-51	21.53834936905749	28.39832684793381	28.21137991839468	21.85194386461402
52-53	21.573331417859382	28.364883745673307	28.192815136420812	21.8689697000465
54-55	21.659082489136786	28.773555864947276	27.984318952379	21.583042693536942
56-57	21.52009308356594	28.48942535371458	28.04547463788759	21.94500692483189
58-59	21.39852511118372	28.15849519216489	28.447100312421526	21.99587938422987
60-61	21.62978380639327	28.575046964856487	28.042198028191606	21.752971200558637
62-63	21.509566122804642	28.6163071117905	28.009393710361678	21.864733055043178
64-65	21.45721351147153	28.3486105253039	28.183354386389375	22.010821576835195
66-67	21.401381826606602	28.327499440372918	28.058311387040792	22.21280734597969
68-69	21.76009759928021	28.469508055775027	28.02606643901422	21.74432790593054
70-71	21.705656611260945	28.289158890819877	28.101301849029582	21.9038826488896
72-73	21.45527687799525	28.43017861598266	28.24180933875255	21.872735167269543
74-75	21.487396435180298	28.433472657375457	28.095887055987312	21.983243851456933
76-77	21.51535902901579	28.362101415658604	28.039415720166964	22.083123835158645
78-79	21.6559640992116	28.534338270258797	28.05193290231433	21.757764728215275
80-81	21.61151231440897	28.233085914631907	28.23773041586648	21.91767135509264
82-83	21.373347237876235	28.351010140559374	28.412603089206357	21.863039532358037
84-85	21.429391155245625	28.273645980301488	28.14800213321812	22.14896073123477
86-87	21.58562056735195	28.407966311537276	28.083097848026583	21.9233152730842
88-89	21.40665854417184	28.286737650208426	28.124070429364856	22.182533376254877
90-91	21.322823427385636	28.186103906900772	28.24967845947448	22.24139420623911
92-93	21.609833002293776	28.327803329087008	28.087314026269627	21.97504964234959
94-95	21.36164301399602	28.058947141958402	28.29660568511615	22.28280415892943
96-97	21.33185670994401	28.112915510847586	28.326314211114767	22.228913568093635
98-99	21.30842294315711	28.268952246846133	28.137484666642763	22.28514014335399
100	21.485676288243106	28.05727528950565	28.060426223594632	22.396622198656615
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	501.0
1	465.5
2	404.5
3	348.0
4	267.5
5	207.5
6	183.5
7	174.5
8	190.5
9	225.0
10	226.0
11	206.0
12	208.0
13	227.0
14	262.0
15	315.5
16	386.0
17	526.0
18	741.5
19	1041.5
20	1509.0
21	2214.0
22	3282.0
23	4971.5
24	7285.0
25	9990.0
26	13422.0
27	17932.5
28	23247.5
29	28771.0
30	33798.5
31	38535.0
32	43218.5
33	47889.0
34	52437.5
35	55925.5
36	58789.0
37	61871.0
38	64110.5
39	64497.0
40	64840.5
41	65409.0
42	65312.5
43	65147.0
44	64174.5
45	63301.0
46	62783.0
47	61110.0
48	60032.0
49	59486.0
50	57564.0
51	55115.5
52	51925.5
53	48951.5
54	47134.0
55	45117.0
56	41562.0
57	37729.0
58	34045.0
59	29602.0
60	25369.0
61	21479.5
62	18051.5
63	14800.0
64	11831.5
65	9444.5
66	7388.0
67	5656.0
68	4368.0
69	3467.5
70	2589.5
71	1776.5
72	1338.5
73	1024.0
74	737.5
75	517.5
76	317.5
77	198.5
78	142.0
79	95.5
80	61.5
81	39.5
82	24.5
83	21.5
84	14.5
85	9.0
86	8.0
87	8.0
88	8.5
89	6.0
90	6.0
91	5.5
92	3.5
93	2.5
94	0.5
95	1.5
96	2.0
97	0.5
98	0.5
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.3327191269484671
2	0.1192410050807037
3	0.06475088998721183
4	0.039801005771955895
5	0.043419279023951894
6	0.00804660767981198
7	0.005940448622679985
8	0.014527097086371963
9	0.017551325476099954
10-11	0.020359537552275948
12-13	0.015202148066221962
14-15	0.017794343828845954
16-17	0.01865840908305395
18-19	0.01846939480869595
20-21	0.02332976186361594
22-23	0.015202148066221962
24-25	0.02392380672588394
26-27	0.018199374416755953
28-29	0.017875349946427952
30-31	0.01914444578854595
32-33	0.024085818961047938
34-35	0.014095064459267965
36-37	0.009126689247571977
38-39	0.012636954342791967
40-41	0.009234697404347975
42-43	0.008181617875781979
44-45	0.008640652542079977
46-47	0.00764157709190198
48-49	0.0036992793695779904
50-51	0.006318477171395984
52-53	0.006750509798499983
54-55	0.002700203919399993
56-57	0.002295173331489994
58-59	0.002538191684235994
60-61	0.0033212508208619916
62-63	0.0025651937234299933
64-65	0.002214167213907994
66-67	0.0017551325476099955
68-69	0.0031862406248919922
70-71	0.0020521549787439947
72-73	0.0016201223516399959
74-75	0.0018091366259979952
76-77	0.003510265095219991
78-79	0.0028892181937579925
80-81	0.0032402447032799917
82-83	0.0021061590571319947
84-85	0.002673201880205993
86-87	0.0021331610963259947
88-89	0.6018484515950644
90-91	0.6131083019389625
92-93	0.6101110755884285
94-95	0.5965290498738465
96-97	0.5954489683060865
98-99	0.5915606746621505
100	0.5933968133273425
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1851712.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	73.3233940472213
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.28334448527792	61.066174852645084
2	11.148746648691969	16.34927887309358
3	2.7606419758725855	6.072589182606156
4	1.0485742101813251	3.0754008000351662
5	0.522634618532095	1.9160672038673994
6	0.29986605900801566	1.319231832361923
7	0.18987069514909666	0.9745374658905931
8	0.13368107134155002	0.7841559896504942
9	0.10256554399419701	0.6768408418158708
>10	0.48404964937660877	6.153399327620415
>50	0.021091070174098427	1.0506249481860066
>100	0.004786812816081043	0.49663353385758746
>500	1.4715958438906278E-4	0.06506514836972632
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	5.400407838799986E-5	0.0
10-11	5.400407838799986E-5	0.0	0.0	1.6201223516399958E-4	0.0
12-13	5.400407838799986E-5	0.0	0.0	2.4301835274599939E-4	0.0
14-15	5.400407838799986E-5	0.0	0.0	2.7002039193999933E-4	0.0
16-17	5.400407838799986E-5	0.0	0.0	2.7002039193999933E-4	0.0
18-19	5.400407838799986E-5	0.0	0.0	3.2402447032799916E-4	0.0
20-21	5.400407838799986E-5	0.0	0.0	3.7802854871599905E-4	0.0
22-23	5.400407838799986E-5	0.0	0.0	5.130387446859987E-4	0.0
24-25	5.400407838799986E-5	0.0	0.0	7.830591366259981E-4	0.0
26-27	5.400407838799986E-5	0.0	0.0	8.640652542079978E-4	0.0
28-29	5.400407838799986E-5	0.0	0.0	0.0015661182732519961	0.0
30-31	8.100611758199979E-5	0.0	0.0	0.004482338506203988	0.0
32-33	1.0800815677599972E-4	0.0	0.0	0.010530795285659974	0.0
34-35	1.0800815677599972E-4	0.0	0.0	0.017821345868039955	0.0
36-37	1.0800815677599972E-4	0.0	0.0	0.033212508208619915	0.0
38-39	1.0800815677599972E-4	0.0	0.0	0.06124062489199184	0.0
40-41	1.0800815677599972E-4	0.0	0.0	0.09866545121487574	0.0
42-43	1.3501019596999964E-4	0.0	0.0	0.13503719800919367	0.0
44-45	1.6201223516399958E-4	0.0	0.0	0.17516222825147754	0.0
46-47	1.6201223516399958E-4	0.0	0.0	0.22255080703694743	0.0
48-49	1.6201223516399958E-4	0.0	0.0	0.2796331178930633	0.0
50-51	1.6201223516399958E-4	0.0	0.0	0.3457071078007811	0.0
52-53	1.6201223516399958E-4	0.0	0.0	0.4111870528462309	0.0
54-55	1.6201223516399958E-4	0.0	0.0	0.4797722323989908	0.0
56-57	1.6201223516399958E-4	5.400407838799986E-5	0.0	0.5609673642553485	0.0
58-59	1.6201223516399958E-4	5.400407838799986E-5	0.0	0.6563115646493624	0.0
60-61	1.6201223516399958E-4	5.400407838799986E-5	0.0	0.7580822503715481	0.0
62-63	1.6201223516399958E-4	5.400407838799986E-5	0.0	0.8618780890332838	0.0
64-65	1.6201223516399958E-4	5.400407838799986E-5	0.0	0.9729914803165935	0.0
66-67	1.6201223516399958E-4	5.400407838799986E-5	0.0	1.0993070196661252	0.0
68-69	1.6201223516399958E-4	8.100611758199979E-5	0.0	1.2370444215947187	0.0
70-71	2.1601631355199944E-4	1.0800815677599972E-4	0.0	1.3875807901012684	0.0
72-73	2.1601631355199944E-4	1.0800815677599972E-4	0.0	1.550646104793834	0.0
74-75	2.1601631355199944E-4	1.0800815677599972E-4	0.0	1.7129553623889677	0.0
76-77	2.1601631355199944E-4	1.0800815677599972E-4	0.0	1.8813130767635573	0.0
78-79	2.1601631355199944E-4	1.0800815677599972E-4	0.0	2.0791030138596067	0.0
80-81	2.1601631355199944E-4	1.0800815677599972E-4	0.0	2.297873535409394	0.0
82-83	2.1601631355199944E-4	1.0800815677599972E-4	0.0	2.5278499032246913	0.0
84-85	2.1601631355199944E-4	1.0800815677599972E-4	0.0	2.755747114022051	0.0
86-87	2.1601631355199944E-4	1.0800815677599972E-4	0.0	2.9951471935160545	0.0
88	2.1601631355199944E-4	1.0800815677599972E-4	0.0	3.191317008260464	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	4910	0.0	28.494349	1
ACACCGT	285	0.0	26.36451	6
ACATGGG	4965	0.0	25.270756	3
TACATGG	5115	0.0	25.097862	2
ACCGTTC	155	1.5586138E-6	24.23834	8
GTATCAA	2885	0.0	24.002436	1
GGTATCA	2215	0.0	22.54322	1
TCAACGC	2755	0.0	22.510473	4
ATCAACG	2840	0.0	22.006931	3
CAACGCA	2845	0.0	21.632643	5
AACGCAG	2915	0.0	20.943506	6
GAGTACA	3385	0.0	20.178698	1
CACCGTT	210	9.608993E-7	20.126478	7
CGTATAC	165	6.870423E-5	19.936104	3
CGACGCG	60	0.0049234694	19.68036	90-91
CGCGCAC	120	0.004926033	19.677954	94
TATCAAC	3290	0.0	19.581429	2
CATGGGG	3225	0.0	19.375559	4
TACACCG	225	1.8538085E-6	18.792326	5
ACGCAGA	3445	0.0	17.721428	7
>>END_MODULE