FastQCFastQC Report
Wed 25 May 2016
SRR1033156_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033156_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1851712
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39160.21147997096740745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33250.17956356064009954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18800.10152766736943974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33950.045.7306981
GTATCAA53400.034.448411
TCAACGC61250.029.5509474
ATCAACG61750.029.15943
CAACGCA61950.029.141155
GTACATG47950.028.257751
AACGCAG63850.028.1168566
TATCAAC65800.027.6504292
TACATGG49050.026.3578782
ACATGGG48100.025.8033243
ACGCAGA73100.024.236877
CGCAGAG74250.024.0500648
GAGTACT53550.022.46516612-13
GCAGAGT81850.021.3002329
CATGGGG33350.021.1452224
TATAACG1805.654372E-620.894612
GTACGAC1656.847277E-519.9448553
AGAGTAC70150.019.72816710-11
CAGAGTA82300.019.0996510-11
GTACTTT64000.018.83373614-15
Produced by FastQC (version 0.11.5)