##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033156_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1851712 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.699819950402656 34.0 31.0 34.0 31.0 34.0 2 32.779807551066256 34.0 31.0 34.0 31.0 34.0 3 32.881383822106244 34.0 31.0 34.0 31.0 34.0 4 36.33319976324612 37.0 37.0 37.0 35.0 37.0 5 36.27195373794629 37.0 37.0 37.0 35.0 37.0 6 36.36036705491999 37.0 37.0 37.0 35.0 37.0 7 36.32553388431894 37.0 37.0 37.0 35.0 37.0 8 36.31766386997546 37.0 37.0 37.0 35.0 37.0 9 38.125293242145645 39.0 39.0 39.0 37.0 39.0 10-11 38.10473739976843 39.0 39.0 39.0 36.0 39.0 12-13 38.06506870398852 39.0 39.0 39.0 35.0 39.0 14-15 39.567295832181244 41.0 40.0 41.0 37.0 41.0 16-17 39.58140547774168 41.0 40.0 41.0 37.0 41.0 18-19 39.602504331127086 41.0 40.0 41.0 37.0 41.0 20-21 39.60307164397055 41.0 40.0 41.0 37.0 41.0 22-23 39.531871586942245 41.0 39.5 41.0 37.0 41.0 24-25 39.48632967761725 41.0 39.0 41.0 36.5 41.0 26-27 39.38219307322089 41.0 39.0 41.0 36.0 41.0 28-29 39.29200437217018 41.0 39.0 41.0 36.0 41.0 30-31 39.19873689861058 41.0 39.0 41.0 36.0 41.0 32-33 39.23525337633498 41.0 39.0 41.0 35.5 41.0 34-35 39.17705831144367 41.0 39.0 41.0 35.0 41.0 36-37 39.095374172657515 41.0 39.0 41.0 35.0 41.0 38-39 39.010706578560814 41.0 39.0 41.0 35.0 41.0 40-41 38.89717407458611 41.0 39.0 41.0 35.0 41.0 42-43 38.78699711402205 41.0 39.0 41.0 35.0 41.0 44-45 38.64622144264335 41.0 39.0 41.0 35.0 41.0 46-47 38.4382503866692 41.0 38.0 41.0 35.0 41.0 48-49 38.25519330219818 40.5 38.0 41.0 34.0 41.0 50-51 38.128219183112705 40.0 38.0 41.0 34.0 41.0 52-53 37.99593376291777 40.0 38.0 41.0 34.0 41.0 54-55 37.86563488274635 40.0 38.0 41.0 34.0 41.0 56-57 37.713525915477135 40.0 37.0 41.0 33.5 41.0 58-59 37.524112821000244 40.0 37.0 41.0 33.0 41.0 60-61 37.33100692764317 40.0 36.5 41.0 33.0 41.0 62-63 37.098634398869805 39.5 36.0 41.0 33.0 41.0 64-65 36.84864330954274 39.0 35.5 41.0 32.5 41.0 66-67 36.56791255875643 39.0 35.0 41.0 32.0 41.0 68-69 36.24578039133515 38.0 35.0 40.5 32.0 41.0 70-71 35.91042694544292 37.0 35.0 40.0 31.0 41.0 72-73 35.55567307443058 37.0 35.0 39.5 31.0 41.0 74-75 35.21788458464383 36.0 35.0 39.0 31.0 41.0 76-77 34.88025135658245 36.0 35.0 39.0 31.0 40.5 78-79 34.53591838255625 35.5 35.0 37.5 31.0 39.5 80-81 34.19229475209968 35.0 34.5 37.0 30.0 39.0 82-83 33.86626511034113 35.0 34.0 37.0 30.0 39.0 84-85 33.58854832716967 35.0 34.0 36.0 30.0 38.0 86-87 33.351894895102475 35.0 34.0 36.0 30.0 37.0 88-89 33.128622053537484 35.0 34.0 36.0 30.0 37.0 90-91 32.923149496249955 35.0 34.0 35.0 29.0 36.5 92-93 32.75409242906024 35.0 34.0 35.0 29.0 36.0 94-95 32.59747682144956 35.0 34.0 35.0 29.0 36.0 96-97 32.469605154581274 35.0 34.0 35.0 29.0 36.0 98-99 32.360815018750216 35.0 34.0 35.0 29.0 36.0 100 32.260558877406424 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0789520499665528 1101 2 0.12499461599504968 1101 3 0.11296017121513557 1101 4 0.030476628001707695 1101 5 0.033330191723976554 1101 6 0.07486069902226689 1101 7 0.07721756686707693 1101 8 0.08706122074444522 1101 9 0.08849078794199272 1101 10-11 0.10233990489707168 1101 12-13 0.12104579406349103 1101 14-15 0.11905489397017277 1101 16-17 0.1529096610352667 1101 18-19 0.1554915583291887 1101 20-21 0.16620861441686685 1101 22-23 0.13322033452996607 1101 24-25 0.1254153785145533 1101 26-27 0.08493004626665623 1101 28-29 0.06990611697723637 1101 30-31 0.03794155709370983 1101 32-33 0.015283452250884011 1101 34-35 0.03576077649567111 1101 36-37 0.11385862072616248 1101 38-39 0.09356277810064739 1101 40-41 0.16540990472059747 1101 42-43 0.0878717214109983 1101 44-45 0.09513831866221523 1101 46-47 0.19466376128610108 1101 48-49 0.1431272985421259 1101 50-51 0.25173747261283097 1101 52-53 0.23158418132480563 1101 54-55 0.17626781859030416 1101 56-57 0.27350008607635345 1101 58-59 0.23323613668274845 1101 60-61 0.2012214594297106 1101 62-63 0.21841611568462582 1101 64-65 0.22784152977972383 1101 66-67 0.22817479220660175 1101 68-69 0.20162888209221563 1101 70-71 0.19726296593077564 1101 72-73 0.26204376191254397 1101 74-75 0.33787165444798006 1101 76-77 0.23423947304290493 1101 78-79 0.3379662971921462 1101 80-81 0.27173877719153694 1101 82-83 0.28161234471346575 1101 84-85 0.2888437164918827 1101 86-87 0.20950795130057998 1101 88-89 0.17804010026696915 1101 90-91 0.21257229645831188 1101 92-93 0.21218968724914333 1101 94-95 0.21175826729366776 1101 96-97 0.23796907413045432 1101 98-99 0.23183485864160502 1101 100 0.11848731257617828 1102 1 0.1309832977019596 1102 2 0.11068421119483673 1102 3 0.10184918088222616 1102 4 0.08355321104154712 1102 5 0.07718535933995696 1102 6 0.124545830259855 1102 7 0.11786426165090802 1102 8 0.12412804973700986 1102 9 0.17494201045891344 1102 10-11 0.14495135901826472 1102 12-13 0.1850657876863977 1102 14-15 0.24843099003232538 1102 16-17 0.15197302610911123 1102 18-19 0.13655323492383076 1102 20-21 0.22237409987275925 1102 22-23 0.22787662393385943 1102 24-25 0.2507913006560969 1102 26-27 0.22967818749018676 1102 28-29 0.2850821391231264 1102 30-31 0.2860682872413989 1102 32-33 0.24486680370094405 1102 34-35 0.286044303315677 1102 36-37 0.3032862407676049 1102 38-39 0.33033031871835306 1102 40-41 0.2996222617796036 1102 42-43 0.33379817473505824 1102 44-45 0.30779339786855076 1102 46-47 0.33314985488437543 1102 48-49 0.16768905485037067 1102 50-51 0.19428841685677867 1102 52-53 0.2486030372170802 1102 54-55 0.23965735001920052 1102 56-57 0.19790893718582936 1102 58-59 0.27063178431960466 1102 60-61 0.17445942629782962 1102 62-63 0.29200355426891633 1102 64-65 0.30812038248346596 1102 66-67 0.296579940256521 1102 68-69 0.3248480075614424 1102 70-71 0.3140714865910965 1102 72-73 0.3513112300633381 1102 74-75 0.27272208272746923 1102 76-77 0.3076049816882005 1102 78-79 0.3084595795087708 1102 80-81 0.2574156906805314 1102 82-83 0.2343077517601415 1102 84-85 0.31102584035234315 1102 86-87 0.2940913389841029 1102 88-89 0.3317098250376205 1102 90-91 0.2813941292916198 1102 92-93 0.18806907330940703 1102 94-95 0.23168938748729317 1102 96-97 0.1755832095276446 1102 98-99 0.28503916462592116 1102 100 0.29992273730479013 1103 1 0.11912661162742211 1103 2 0.13110733408462494 1103 3 0.14369325939175326 1103 4 0.07170102559477698 1103 5 0.027408271077710822 1103 6 0.12137927567334117 1103 7 0.10961998267470818 1103 8 0.1580360696814722 1103 9 0.1262206823614136 1103 10-11 0.14040361984329053 1103 12-13 0.08809228564049931 1103 14-15 0.12437082273672928 1103 16-17 0.1820816451306797 1103 18-19 0.13171455194108006 1103 20-21 0.12639037298129807 1103 22-23 0.12224790656050288 1103 24-25 0.1325764325564336 1103 26-27 0.16426773314164933 1103 28-29 0.20188687343510026 1103 30-31 0.15961632665179337 1103 32-33 0.15203039484185865 1103 34-35 0.23718280244052892 1103 36-37 0.22498845658635958 1103 38-39 0.14815274659506628 1103 40-41 0.23233090797915423 1103 42-43 0.1285220016972488 1103 44-45 0.1389339818003208 1103 46-47 0.010882548954349147 1103 48-49 -0.010492467953007178 1103 50-51 -9.118668860281787E-4 1103 52-53 0.025383369415678203 1103 54-55 -0.0019779429164969997 1103 56-57 -0.028587840945419885 1103 58-59 -0.05269730680910811 1103 60-61 -0.02495525980225466 1103 62-63 -0.15145222807956316 1103 64-65 -0.08408784036672046 1103 66-67 -0.05330191100557613 1103 68-69 -0.06699479335053127 1103 70-71 -0.03127554665315557 1103 72-73 0.025643554675319535 1103 74-75 0.01051304105340023 1103 76-77 -0.027826515272849406 1103 78-79 -0.08588774816364264 1103 80-81 -0.0652767068009723 1103 82-83 -0.032718268256324734 1103 84-85 0.02513435019739063 1103 86-87 -0.018290846195540666 1103 88-89 0.003461039626387219 1103 90-91 -0.05214100638343666 1103 92-93 0.033640629806512834 1103 94-95 0.05080037412943739 1103 96-97 0.0031073212779446635 1103 98-99 -0.006450598333266555 1103 100 -0.10085264826835072 1104 1 0.09564488784702263 1104 2 0.15035603718998658 1104 3 0.0576385029354185 1104 4 0.09211696601139607 1104 5 0.0037231520536451512 1104 6 0.07223704226501582 1104 7 0.09473727845495716 1104 8 0.10587775905036523 1104 9 0.1379493319669649 1104 10-11 0.07130822733117981 1104 12-13 0.09463499928441621 1104 14-15 0.0995809561620149 1104 16-17 0.121065071703498 1104 18-19 0.11521989756516149 1104 20-21 0.10372751502388411 1104 22-23 0.052364428896055415 1104 24-25 0.07712651842093265 1104 26-27 0.03237477331663996 1104 28-29 0.1416340320078291 1104 30-31 0.10578678986303913 1104 32-33 0.1471444097311263 1104 34-35 0.06730770911652684 1104 36-37 0.04450260191140387 1104 38-39 0.04822597745280888 1104 40-41 0.06577362815058052 1104 42-43 0.06760648314007511 1104 44-45 0.1430677330270953 1104 46-47 0.13656374090761147 1104 48-49 0.08312533495843155 1104 50-51 0.17394948083314432 1104 52-53 0.15759784798611776 1104 54-55 0.1942907541984198 1104 56-57 0.17661082150232232 1104 58-59 0.13807429104478786 1104 60-61 0.22263031439008785 1104 62-63 0.11543075552297921 1104 64-65 0.15823159671980846 1104 66-67 0.25688626244657087 1104 68-69 0.2775159703857497 1104 70-71 0.27143392366649266 1104 72-73 0.23400112808436546 1104 74-75 0.16873461531830003 1104 76-77 0.18807267953050655 1104 78-79 0.20297741921535817 1104 80-81 0.16843021293659888 1104 82-83 0.2046876168079379 1104 84-85 0.17099423572757644 1104 86-87 0.12199880919949635 1104 88-89 0.1613671655797475 1104 90-91 0.20838980378238858 1104 92-93 0.13089955038052636 1104 94-95 0.07847891023216391 1104 96-97 0.10679247458210739 1104 98-99 0.1454869341881917 1104 100 0.1908948325372961 1105 1 0.02286066974298251 1105 2 -0.008844038850398306 1105 3 -0.0299042251397168 1105 4 0.02960163362728707 1105 5 0.0028568465971119394 1105 6 -0.09343594397656574 1105 7 -0.07947335615534001 1105 8 -0.06484334168912653 1105 9 -0.011659573768504572 1105 10-11 -0.0453733104317493 1105 12-13 0.012829501685544642 1105 14-15 -0.049797671929908915 1105 16-17 -0.03185219509523307 1105 18-19 -0.05114948453254442 1105 20-21 -0.04628418772304599 1105 22-23 0.007950306592462653 1105 24-25 -0.015121757827628812 1105 26-27 -0.024877276106316515 1105 28-29 0.013845461086297917 1105 30-31 0.04606576302116849 1105 32-33 0.009272589973207346 1105 34-35 0.015041757874129758 1105 36-37 -0.05240026336608139 1105 38-39 -0.05893348016607547 1105 40-41 0.004371704999165615 1105 42-43 -0.011667471297776899 1105 44-45 0.048232215684635094 1105 46-47 0.13607303593798292 1105 48-49 0.12188409107181286 1105 50-51 0.14907709159648164 1105 52-53 0.11858196109508867 1105 54-55 0.1662560135227764 1105 56-57 0.10993888493669601 1105 58-59 0.15349581931914713 1105 60-61 0.10067903792532462 1105 62-63 0.12049521540686925 1105 64-65 0.05360917650932606 1105 66-67 0.05247416202601585 1105 68-69 0.11617066545365162 1105 70-71 0.1665733965099392 1105 72-73 0.1781185885003751 1105 74-75 0.10769476306960257 1105 76-77 0.09321882513741286 1105 78-79 0.053956191915872864 1105 80-81 0.10603451778931117 1105 82-83 0.09080024723107272 1105 84-85 0.0935145651296807 1105 86-87 0.054301027968890025 1105 88-89 0.08356724903736534 1105 90-91 -0.040967781342054366 1105 92-93 0.05961697876507088 1105 94-95 0.06292613812337322 1105 96-97 0.019463678764545023 1105 98-99 0.0629880625692465 1105 100 0.033487941487400974 1106 1 0.009792999588533746 1106 2 0.04843467578930927 1106 3 0.027442620710303345 1106 4 -0.006093789697665386 1106 5 -3.631332183644531E-4 1106 6 0.02641283594168442 1106 7 0.021566919011533514 1106 8 0.05946205152027062 1106 9 0.054977430684211015 1106 10-11 0.07453651453085541 1106 12-13 0.05150501535456442 1106 14-15 0.0801686577299705 1106 16-17 0.09324931910791179 1106 18-19 0.13472363903120765 1106 20-21 0.0441977651774863 1106 22-23 0.09973305852646774 1106 24-25 0.05735567760959981 1106 26-27 0.0661792413330673 1106 28-29 0.03957389944354617 1106 30-31 0.06840727409339564 1106 32-33 0.03578915300849417 1106 34-35 0.09788020811272702 1106 36-37 0.02960696010246977 1106 38-39 0.036244195368652754 1106 40-41 0.050153537941959314 1106 42-43 0.1428784926951323 1106 44-45 0.22038275133377283 1106 46-47 0.21225159535814697 1106 48-49 0.11486878677364132 1106 50-51 0.1730387992558633 1106 52-53 0.15202658407335434 1106 54-55 0.14077214540711225 1106 56-57 0.1385903342461745 1106 58-59 0.1435545135766958 1106 60-61 0.10794294312829322 1106 62-63 0.1512693289795095 1106 64-65 0.07915968058927092 1106 66-67 0.1075423829510953 1106 68-69 0.15270459674670178 1106 70-71 0.07117247424219642 1106 72-73 0.16674236665682685 1106 74-75 0.16396438167264193 1106 76-77 0.10863552680149269 1106 78-79 0.1359031504684367 1106 80-81 0.19012624424198066 1106 82-83 0.16004340049950372 1106 84-85 0.09517149276055648 1106 86-87 0.08419996754041392 1106 88-89 0.02573204283253716 1106 90-91 0.00935433365875582 1106 92-93 0.05347548324339613 1106 94-95 0.03791749820176449 1106 96-97 0.06307929027726544 1106 98-99 0.004343473188505698 1106 100 0.029266926961668105 1107 1 -0.03296385237663202 1107 2 -0.03752254670652633 1107 3 -0.006329895066279789 1107 4 0.050850997026195444 1107 5 -0.05696866979586446 1107 6 -0.09564644518801657 1107 7 -0.08717987911057179 1107 8 -0.07885076204842534 1107 9 -0.07393472858756667 1107 10-11 -0.14777332562490386 1107 12-13 -0.06299159422178491 1107 14-15 -0.012315981882231597 1107 16-17 -0.044145604959368256 1107 18-19 -0.05857552834517321 1107 20-21 -0.03657858875990172 1107 22-23 -0.023926516166682177 1107 24-25 0.026747796503805432 1107 26-27 0.011204472905298246 1107 28-29 0.005889659224720845 1107 30-31 -0.09025104068968659 1107 32-33 -0.05022826309163264 1107 34-35 -0.008679723624197777 1107 36-37 0.08844923056996379 1107 38-39 0.0957620068198679 1107 40-41 0.03939865347653182 1107 42-43 0.001667948941623365 1107 44-45 0.016452675649759385 1107 46-47 0.015346919234367817 1107 48-49 0.0056889850775689865 1107 50-51 -0.11204453299659178 1107 52-53 0.0022997786650478247 1107 54-55 -0.0891807083930729 1107 56-57 -0.1368718437070271 1107 58-59 -0.07429174415040762 1107 60-61 -0.09877599794783265 1107 62-63 -0.082018477168873 1107 64-65 -0.12414780685287496 1107 66-67 -0.12671040978658255 1107 68-69 -0.00885352603388867 1107 70-71 -0.03165010208689267 1107 72-73 -0.02059435976563151 1107 74-75 -0.015364265710324787 1107 76-77 -0.028551175247585547 1107 78-79 -0.005441758506371741 1107 80-81 0.06917922254722697 1107 82-83 0.026557345833893464 1107 84-85 0.009818284120328258 1107 86-87 0.051161108539147904 1107 88-89 -0.01822213296023989 1107 90-91 -0.018784413354708818 1107 92-93 -0.04226716668306807 1107 94-95 -0.032001969555807364 1107 96-97 -0.06065187450801801 1107 98-99 -0.10659252098757577 1107 100 -0.04536067548299627 1108 1 -0.01844563530694643 1108 2 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8 -0.04200039062996552 2214 9 -0.08655780605484154 2214 10-11 -0.08671671437623019 2214 12-13 -0.03019249977538152 2214 14-15 -0.06026668580865646 2214 16-17 -0.10769792190312444 2214 18-19 -0.12231337058075553 2214 20-21 -0.11807960043305599 2214 22-23 -0.08823236689183034 2214 24-25 -0.10442209383931811 2214 26-27 -0.20972688896241465 2214 28-29 -0.13939871714598695 2214 30-31 -0.048153608783067625 2214 32-33 -0.019341697828103577 2214 34-35 -0.12438462634793268 2214 36-37 -0.1705764503638889 2214 38-39 -0.12833952837645057 2214 40-41 -0.0477785944895075 2214 42-43 -0.03151968459216192 2214 44-45 -0.06443969081645662 2214 46-47 -0.0030417047486537285 2214 48-49 -0.18066562404871434 2214 50-51 -0.12123788283740566 2214 52-53 -0.11630668755356055 2214 54-55 -0.05526772643339939 2214 56-57 -0.12850616984781027 2214 58-59 -0.21015274167427833 2214 60-61 -0.3216151914391716 2214 62-63 -0.21860555888093813 2214 64-65 -0.18190848601806664 2214 66-67 -0.2515067875722252 2214 68-69 -0.22797168662816603 2214 70-71 -0.17255548588597236 2214 72-73 -0.25110461091525593 2214 74-75 -0.17971596008598567 2214 76-77 -0.15960040057478864 2214 78-79 -0.31894005351875165 2214 80-81 -0.24306210748460444 2214 82-83 -0.24705640726126177 2214 84-85 -0.2890196642348144 2214 86-87 -0.1820341656030564 2214 88-89 -0.27881323306635863 2214 90-91 -0.15818776673284418 2214 92-93 -0.04643514687408157 2214 94-95 -0.15744779905704576 2214 96-97 -0.4003164234998806 2214 98-99 -0.3444329454658188 2214 100 -0.54828374587564 2215 1 -0.09933814889771497 2215 2 -0.11385238518941776 2215 3 -0.13248750698944178 2215 4 -0.04957434665187321 2215 5 -0.07785561110846828 2215 6 -0.10575513585096985 2215 7 -0.13698618105912175 2215 8 -0.11755071580803644 2215 9 -0.12716639075598835 2215 10-11 -0.1332568444474589 2215 12-13 -0.18708629971344237 2215 14-15 -0.16152488339304938 2215 16-17 -0.16339169706571255 2215 18-19 -0.18735463412984643 2215 20-21 -0.16580870541911708 2215 22-23 -0.1595828531104715 2215 24-25 -0.12629184918152703 2215 26-27 -0.1394011162773694 2215 28-29 -0.08887394786756886 2215 30-31 -0.08808017428322756 2215 32-33 -0.14047661266241818 2215 34-35 -0.13683073350185282 2215 36-37 -0.12211215801332287 2215 38-39 -0.1268114763479602 2215 40-41 -0.11618114636315369 2215 42-43 -0.011777696360503853 2215 44-45 -0.05882883915960235 2215 46-47 -0.15906732258699208 2215 48-49 -0.02290255189015511 2215 50-51 0.025971531842060358 2215 52-53 0.0026059665189137604 2215 54-55 -0.017312941670311943 2215 56-57 -0.12203838415564405 2215 58-59 -0.11804308543396047 2215 60-61 -0.0923885578064656 2215 62-63 -0.05414245575303056 2215 64-65 -0.02629285269505033 2215 66-67 -0.0371138733572991 2215 68-69 -0.07004103317508736 2215 70-71 -0.008321654978566073 2215 72-73 0.027425262110448045 2215 74-75 -0.01135780585867252 2215 76-77 0.024824597876744292 2215 78-79 0.01200059683739596 2215 80-81 -0.050681655332240894 2215 82-83 0.04447043076908841 2215 84-85 -0.029484898053340203 2215 86-87 -0.10487402933795664 2215 88-89 -0.09707087320262531 2215 90-91 -0.0538103867390376 2215 92-93 0.04857066297417134 2215 94-95 -0.03138811494431337 2215 96-97 0.05609465113622747 2215 98-99 0.03204824375685433 2215 100 -0.005527771889887845 2216 1 -0.06672747993674477 2216 2 -0.13578257834617347 2216 3 -0.16728998806541284 2216 4 -0.11009159499580079 2216 5 -0.07166991485780017 2216 6 -0.08733687517641187 2216 7 -0.041646579258909355 2216 8 -0.037646567541507636 2216 9 -0.0929592453352015 2216 10-11 -0.1435740457105652 2216 12-13 -0.11364053575738353 2216 14-15 -0.18049840994658695 2216 16-17 -0.1982970223529037 2216 18-19 -0.09623169142034271 2216 20-21 -0.186213693386577 2216 22-23 -0.15755554530262827 2216 24-25 -0.278942513629417 2216 26-27 -0.3292828709714044 2216 28-29 -0.3272682223933572 2216 30-31 -0.21567120998246736 2216 32-33 -0.24190649542930487 2216 34-35 -0.24762756487866966 2216 36-37 -0.25360853432191277 2216 38-39 -0.15950618504811587 2216 40-41 -0.2585676799467791 2216 42-43 -0.2489316186251429 2216 44-45 -0.1701291660788229 2216 46-47 -0.15072770175014938 2216 48-49 -0.0933701517848462 2216 50-51 -0.008611795880888451 2216 52-53 0.04684485667432625 2216 54-55 0.09658834553061979 2216 56-57 0.09429898757347388 2216 58-59 -0.03318746431295949 2216 60-61 0.06242438877072232 2216 62-63 0.06843683991966998 2216 64-65 0.056943448014919795 2216 66-67 0.10841908948924583 2216 68-69 0.2199501124722829 2216 70-71 0.21592715279469132 2216 72-73 0.11293448953451701 2216 74-75 0.09063342522135542 2216 76-77 0.12181393225878878 2216 78-79 0.03450114588154918 2216 80-81 0.05317033629350476 2216 82-83 0.06355780563229274 2216 84-85 0.09356576305154363 2216 86-87 0.09349782839994702 2216 88-89 0.12206808627396981 2216 90-91 0.07268311782487302 2216 92-93 0.05748161474510738 2216 94-95 0.02864899254715425 2216 96-97 0.09317275191241237 2216 98-99 0.07122594508890501 2216 100 0.1379560142443026 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 7.0 4 13.0 5 22.0 6 37.0 7 104.0 8 223.0 9 445.0 10 779.0 11 1240.0 12 1976.0 13 2991.0 14 3468.0 15 4577.0 16 5505.0 17 8252.0 18 9713.0 19 5323.0 20 3645.0 21 3324.0 22 3518.0 23 3886.0 24 4306.0 25 5180.0 26 6485.0 27 8498.0 28 11151.0 29 14113.0 30 18323.0 31 23059.0 32 29941.0 33 39441.0 34 54894.0 35 84225.0 36 153354.0 37 356639.0 38 657951.0 39 323639.0 40 1457.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29568519344333 19.93081174578722 13.101120255131688 23.672382805637763 2 15.690593830821484 20.182330834173555 40.25838032196552 23.868695013039446 3 18.254404572633327 25.485550668786505 30.374809905644074 25.885234852936094 4 12.011747017881907 16.75796995632131 39.18147824922342 32.04880477657336 5 12.101523698281484 38.16960631716458 36.16621023611029 13.562659748443643 6 30.635067732061426 38.713285247998066 17.495145022865376 13.156501997075132 7 26.91629910743459 32.82174776912654 22.725826826000244 17.536126297438635 8 25.236052846459263 36.55168428647343 20.108181437205946 18.10408142986136 9 24.98220563695179 16.070388991796772 21.345729082847747 37.60167628840369 10-11 23.77101745985928 26.727860735878757 29.211869388447237 20.289252415814726 12-13 24.706028799294923 24.0657294438876 29.417533612138392 21.810708144679086 14-15 21.65554496774739 26.004847950044024 26.954537439842785 25.385069642365803 16-17 20.83471943801196 29.583002108319224 27.467392337469327 22.114886116199493 18-19 20.679031263779482 28.087501751757767 30.160313850186704 21.073153134276048 20-21 21.761834453737947 27.423352011543912 29.912048957937305 20.90276457678084 22-23 21.31846275023161 27.299989550196724 29.982375745223532 21.39917195434813 24-25 21.22712453509214 27.412133966909526 29.957212545586216 21.40352895241212 26-27 21.002388632636077 27.333453224006 30.109003303474076 21.555154839883848 28-29 21.04149592169878 27.40877661764964 30.16120125245231 21.38852620819927 30-31 21.322027278127177 27.859664021942216 29.616645962128928 21.201662737801676 32-33 20.94974254927 27.540637890092412 30.1886909662127 21.32092859442489 34-35 21.062463198949853 27.306055089483948 30.22442563351826 21.407056078047937 36-37 21.484001936016387 27.689814954862296 29.53297385945731 21.293209249664006 38-39 21.345708095272308 27.849237349432084 29.641467534480924 21.163587020814685 40-41 21.150797605899303 27.643873334905344 29.835368437770693 21.369960621424656 42-43 21.08569817337983 27.588306087437502 29.664725315564084 21.661270423618582 44-45 21.31945078385755 27.681247646914887 29.506431450883248 21.492870118344314 46-47 21.43756260402497 27.992185451317464 29.132419277435112 21.437832667222455 48-49 21.456621786351405 27.956373071378763 28.980425240491986 21.606579901777838 50-51 21.44948607225152 27.985656524032127 28.874603141598215 21.69025426211814 52-53 21.484726887806353 27.93853490457005 28.83645132370573 21.740286883917868 54-55 21.545303255286704 28.332062844507018 28.681697049036547 21.440936851169727 56-57 21.38498723179961 28.002228258213325 28.722712815266 21.890071694721065 58-59 21.301612495142965 27.63313406550915 29.124442976720527 21.94081046262736 60-61 21.48338963279788 28.069643922763078 28.80707240084139 21.639894043597657 62-63 21.366469891574784 28.02214300334127 28.77399630931943 21.83739079576452 64-65 21.32051602276333 27.836120243294875 28.916149676304403 21.927214057637393 66-67 21.239532580366777 27.91443313110453 28.751961712979114 22.094072575549575 68-69 21.670712509349542 27.94925730209349 28.71301699253927 21.667013196017702 70-71 21.573999293083368 27.820598708162507 28.789085293622875 21.816316705131246 72-73 21.40362521318271 27.95766372270208 28.830160209710222 21.808550854404984 74-75 21.398849705675865 27.988335043473565 28.693794891181078 21.919020359669492 76-77 21.408125004894142 27.99141439220696 28.57841441677893 22.02204618611997 78-79 21.551797229697293 28.113161287047493 28.61329332709046 21.721748156164757 80-81 21.49113948386832 27.818143131331354 28.7635996798614 21.927117704938926 82-83 21.29318254601305 27.954509983512477 28.865806233505047 21.886501236969426 84-85 21.3005351278577 27.853083668204526 28.692579333555468 22.1538018703823 86-87 21.426650422204794 28.00468540396218 28.569596286239513 21.999067887593508 88-89 21.167390276509057 27.848290621946237 28.6980990289254 22.286220072619304 90-91 21.12612544499361 27.776862708671757 28.742050599661283 22.354961246673348 92-93 21.291350922821692 27.979188988352398 28.60369215083123 22.125767937994677 94-95 21.103524738188227 27.700446937752737 28.73268089206097 22.463347431998066 96-97 20.986254881968687 27.73765574776207 28.778881381121902 22.49720798914734 98-99 20.911162210970172 27.893808540420974 28.642359070957042 22.552670177651816 100 21.05478605744306 27.650466163204644 28.607472436318393 22.687275343033907 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 101.0 1 74.5 2 171.0 3 327.0 4 230.0 5 130.0 6 307.0 7 710.0 8 1262.0 9 1483.5 10 1148.5 11 796.0 12 760.5 13 864.0 14 987.0 15 1145.0 16 1291.0 17 1479.0 18 1730.0 19 2082.0 20 2601.0 21 3237.5 22 4158.0 23 5722.0 24 7913.0 25 10517.0 26 13924.0 27 18148.0 28 23256.5 29 28535.5 30 33250.0 31 37825.0 32 42564.5 33 47450.5 34 51981.0 35 55266.5 36 58035.5 37 61279.0 38 63419.5 39 63941.5 40 64183.0 41 64591.0 42 64921.0 43 65194.5 44 64438.5 45 63127.0 46 62128.5 47 60951.0 48 60234.0 49 59138.5 50 57229.0 51 54925.5 52 51354.0 53 48331.5 54 46740.5 55 44915.5 56 41443.0 57 37610.0 58 33690.5 59 29278.5 60 25242.5 61 21227.5 62 17774.5 63 14555.5 64 11560.5 65 9259.5 66 7296.0 67 5495.0 68 4157.5 69 3224.5 70 2315.5 71 1565.5 72 1133.0 73 838.0 74 624.0 75 416.0 76 238.5 77 147.5 78 84.5 79 35.0 80 17.0 81 9.5 82 6.0 83 2.5 84 0.5 85 1.5 86 2.0 87 1.0 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09110488024055577 2 0.0012420938029239967 3 0.0 4 4.3203262710399894E-4 5 0.040773079182939896 6 2.1601631355199947E-4 7 0.004482338506203988 8 4.3203262710399894E-4 9 1.0800815677599973E-4 10-11 4.0503058790999897E-4 12-13 0.0 14-15 8.100611758199979E-5 16-17 0.0 18-19 2.7002039193999934E-5 20-21 0.0 22-23 1.3501019596999964E-4 24-25 5.400407838799987E-5 26-27 0.0013501019596999966 28-29 0.00780358932706598 30-31 0.03610172640237791 32-33 0.02613797393979193 34-35 0.029270210486295925 36-37 0.026353990253343933 38-39 0.025435920920747937 40-41 0.03124135934745792 42-43 0.02289772923651194 44-45 0.022627708844571943 46-47 0.01585019700687796 48-49 0.010530795285659974 50-51 0.00747956485673798 52-53 0.004968375211695987 54-55 0.0033752548992499916 56-57 0.002241169253101994 58-59 8.910672934019977E-4 60-61 3.78028548715999E-4 62-63 0.0011340856461479973 64-65 0.002673201880205993 66-67 7.56057097431998E-4 68-69 9.180693325959977E-4 70-71 4.5903466629799885E-4 72-73 7.56057097431998E-4 74-75 6.480489406559983E-4 76-77 4.5903466629799885E-4 78-79 5.400407838799987E-5 80-81 7.56057097431998E-4 82-83 4.860367054919987E-4 84-85 2.700203919399993E-4 86-87 2.1601631355199947E-4 88-89 2.7002039193999934E-5 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1851712.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.4433065165477 #Duplication Level Percentage of deduplicated Percentage of total 1 82.55624230120839 58.98090923579612 2 11.52908552623538 16.473519822126562 3 2.905152217263866 6.226610410056317 4 1.096340866751378 3.1330486635974513 5 0.5547503970220785 1.9816601327312449 6 0.33090046625322544 1.4184374062198644 7 0.21354043467909475 1.067922430991878 8 0.1488855144460589 0.8509498755554943 9 0.10691492399460006 0.6874520117525652 >10 0.5243356807023261 6.473066741106086 >50 0.025915700158192393 1.2398413991704142 >100 0.0074827738465085905 0.8630034128574622 >500 2.2659871946871358E-4 0.11138005698627226 >1k 2.265987194687132E-4 0.4921984010522229 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3916 0.21147997096740745 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3325 0.17956356064009954 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1880 0.10152766736943974 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 5.400407838799986E-5 0.0 12-13 0.0 0.0 0.0 5.400407838799986E-5 0.0 14-15 0.0 0.0 0.0 5.400407838799986E-5 0.0 16-17 0.0 0.0 0.0 5.400407838799986E-5 0.0 18-19 0.0 0.0 0.0 2.1601631355199944E-4 0.0 20-21 0.0 0.0 0.0 2.4301835274599939E-4 0.0 22-23 8.100611758199979E-5 0.0 0.0 4.0503058790999897E-4 0.0 24-25 1.6201223516399958E-4 0.0 0.0 8.100611758199979E-4 0.0 26-27 1.6201223516399958E-4 0.0 0.0 8.640652542079978E-4 0.0 28-29 1.6201223516399958E-4 0.0 0.0 0.0015661182732519961 0.0 30-31 1.6201223516399958E-4 0.0 0.0 0.004509340545397989 0.0 32-33 1.8901427435799952E-4 0.0 0.0 0.010530795285659974 0.0 34-35 2.1601631355199944E-4 0.0 0.0 0.017902351985621956 0.0 36-37 2.1601631355199944E-4 0.0 0.0 0.03350953063975391 0.0 38-39 4.320326271039989E-4 0.0 0.0 0.06121362285279784 0.0 40-41 4.320326271039989E-4 0.0 0.0 0.09871945529326374 0.0 42-43 4.320326271039989E-4 0.0 0.0 0.13511820412677567 0.0 44-45 4.320326271039989E-4 2.700203919399993E-5 0.0 0.17527023640825357 0.0 46-47 4.320326271039989E-4 5.400407838799986E-5 0.0 0.2227668233504994 0.0 48-49 4.320326271039989E-4 5.400407838799986E-5 0.0 0.2800651505201673 0.0 50-51 4.320326271039989E-4 5.400407838799986E-5 0.0 0.3467601873293471 0.0 52-53 4.320326271039989E-4 5.400407838799986E-5 0.0 0.41283417723706495 0.0 54-55 4.320326271039989E-4 5.400407838799986E-5 0.0 0.4820674057304808 0.0 56-57 4.320326271039989E-4 5.400407838799986E-5 0.0 0.5643156171154046 0.0 58-59 4.320326271039989E-4 5.400407838799986E-5 0.0 0.6604428766460443 0.0 60-61 4.320326271039989E-4 5.400407838799986E-5 0.0 0.7625105847993641 0.0 62-63 4.320326271039989E-4 5.400407838799986E-5 0.0 0.8666844520098158 0.0 64-65 4.320326271039989E-4 5.400407838799986E-5 0.0 0.9784188901945874 0.0 66-67 4.320326271039989E-4 5.400407838799986E-5 0.0 1.105517488680745 0.0 68-69 4.320326271039989E-4 5.400407838799986E-5 0.0 1.2440379497459648 0.0 70-71 4.320326271039989E-4 5.400407838799986E-5 0.0 1.3956003957418863 0.0 72-73 4.320326271039989E-4 5.400407838799986E-5 0.0 1.559340761414302 0.0 74-75 4.320326271039989E-4 5.400407838799986E-5 0.0 1.7222710659108977 0.0 76-77 5.130387446859987E-4 5.400407838799986E-5 0.0 1.8918708740884111 0.0 78-79 5.400407838799987E-4 5.400407838799986E-5 0.0 2.0917399682023987 0.0 80-81 5.400407838799987E-4 5.400407838799986E-5 0.0 2.313993752808212 0.0 82-83 5.400407838799987E-4 5.400407838799986E-5 0.0 2.5491005080703695 0.0 84-85 5.940448622679985E-4 8.100611758199979E-5 0.0 2.780994020668441 0.0 86-87 6.480489406559983E-4 1.0800815677599972E-4 0.0 3.0236073428265304 0.0 88 6.480489406559983E-4 1.0800815677599972E-4 0.0 3.2225313655687278 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3395 0.0 45.730698 1 GTATCAA 5340 0.0 34.44841 1 TCAACGC 6125 0.0 29.550947 4 ATCAACG 6175 0.0 29.1594 3 CAACGCA 6195 0.0 29.14115 5 GTACATG 4795 0.0 28.25775 1 AACGCAG 6385 0.0 28.116856 6 TATCAAC 6580 0.0 27.650429 2 TACATGG 4905 0.0 26.357878 2 ACATGGG 4810 0.0 25.803324 3 ACGCAGA 7310 0.0 24.23687 7 CGCAGAG 7425 0.0 24.050064 8 GAGTACT 5355 0.0 22.465166 12-13 GCAGAGT 8185 0.0 21.300232 9 CATGGGG 3335 0.0 21.145222 4 TATAACG 180 5.654372E-6 20.89461 2 GTACGAC 165 6.847277E-5 19.944855 3 AGAGTAC 7015 0.0 19.728167 10-11 CAGAGTA 8230 0.0 19.09965 10-11 GTACTTT 6400 0.0 18.833736 14-15 >>END_MODULE