FastQCFastQC Report
Wed 25 May 2016
SRR1033155_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033155_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3837729
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG108850.026.529541
TACATGG109800.025.2218482
GTATCAA65050.024.6947561
ACATGGG106400.024.5967273
TCAACGC62950.024.1034934
ATCAACG63350.024.0304623
CAACGCA64550.023.5054265
AACGCAG65750.022.999296
TCTAACG1754.454603E-621.4790823
TATCAAC75550.020.7862422
GTCGTAC2601.6418198E-819.9304451
GGTATCA48650.019.4630491
ACGCAGA78550.018.7133887
TAGACCG1801.344115E-418.2678495
CATGGGG70650.017.8860034
CGCAGAG80650.017.8767418
GTATACG3008.456482E-817.2730521
TATACTG19450.017.1474915
GAGTACA75300.017.1416741
GTATTAG18050.016.442241
Produced by FastQC (version 0.11.5)