##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033155_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3837729
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.739860735346348	33.0	31.0	34.0	30.0	34.0
2	31.829781362884145	34.0	31.0	34.0	30.0	34.0
3	32.00981413747557	34.0	31.0	34.0	30.0	34.0
4	35.39222545416834	37.0	35.0	37.0	33.0	37.0
5	35.469274406817156	37.0	35.0	37.0	35.0	37.0
6	35.53145727590458	37.0	36.0	37.0	35.0	37.0
7	35.50667256598889	37.0	37.0	37.0	35.0	37.0
8	35.494585730258706	37.0	37.0	37.0	35.0	37.0
9	37.19158387681882	39.0	38.0	39.0	35.0	39.0
10-11	37.178235357421016	39.0	38.0	39.0	35.0	39.0
12-13	37.13146941850245	39.0	38.0	39.0	35.0	39.0
14-15	38.60247388494602	41.0	39.0	41.0	35.0	41.0
16-17	38.607187479887195	41.0	39.0	41.0	35.0	41.0
18-19	38.59160300271333	41.0	39.0	41.0	35.0	41.0
20-21	38.61467341753417	41.0	39.0	41.0	35.0	41.0
22-23	38.57167715594301	41.0	39.0	41.0	34.0	41.0
24-25	38.55421200923776	41.0	39.0	41.0	34.0	41.0
26-27	38.50502862500192	41.0	39.0	41.0	34.0	41.0
28-29	38.473946571005925	40.5	39.0	41.0	34.0	41.0
30-31	38.47271211698377	41.0	39.0	41.0	34.0	41.0
32-33	38.539436604304264	41.0	39.0	41.0	34.0	41.0
34-35	38.512355093337746	41.0	39.0	41.0	34.0	41.0
36-37	38.44016995467893	41.0	39.0	41.0	34.0	41.0
38-39	38.36478565839329	41.0	39.0	41.0	34.0	41.0
40-41	38.30323167685889	41.0	39.0	41.0	34.0	41.0
42-43	38.19355548554887	40.5	38.0	41.0	33.0	41.0
44-45	38.063669816185566	40.0	38.0	41.0	33.0	41.0
46-47	37.93798572020067	40.0	38.0	41.0	33.0	41.0
48-49	37.81597580235603	40.0	38.0	41.0	33.0	41.0
50-51	37.69090456882182	40.0	38.0	41.0	33.0	41.0
52-53	37.543701496379754	40.0	37.0	41.0	32.0	41.0
54-55	37.38476713702296	40.0	37.0	41.0	32.0	41.0
56-57	37.22088076047058	40.0	37.0	41.0	32.0	41.0
58-59	37.0176108057656	40.0	36.0	41.0	31.0	41.0
60-61	36.780265750916755	39.0	36.0	41.0	31.0	41.0
62-63	36.51588713012305	39.0	35.0	41.0	31.0	41.0
64-65	36.23333890954781	39.0	35.0	41.0	30.5	41.0
66-67	35.92569094899614	38.0	35.0	40.0	30.0	41.0
68-69	35.603741692026716	37.5	35.0	40.0	30.0	41.0
70-71	35.244018011693896	37.0	35.0	39.5	30.0	41.0
72-73	34.8643594948992	36.5	34.5	39.0	29.0	41.0
74-75	34.48384448198401	36.0	34.0	39.0	29.0	41.0
76-77	34.14811780612962	35.5	34.0	38.5	29.0	40.0
78-79	33.77091126027919	35.0	34.0	37.0	29.0	39.0
80-81	33.40671644610654	35.0	34.0	37.0	28.5	39.0
82-83	33.189626208624944	35.0	34.0	36.5	29.0	39.0
84-85	32.92078922195913	35.0	34.0	36.0	28.5	37.0
86-87	32.67245993659271	35.0	34.0	36.0	28.0	37.0
88-89	32.31722784490515	35.0	34.0	35.0	27.0	36.5
90-91	32.12637786565961	35.0	34.0	35.0	27.0	36.0
92-93	31.948834584203315	35.0	33.5	35.0	26.5	36.0
94-95	31.78157850645525	35.0	33.0	35.0	26.0	36.0
96-97	31.607266563115843	35.0	33.0	35.0	25.5	36.0
98-99	31.412540202812654	35.0	33.0	35.0	25.0	35.0
100	31.249459771651413	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.22018220184585857
1101	2	0.22959242875383623
1101	3	0.25764935846223835
1101	4	0.1628169235425858
1101	5	0.23816363208803182
1101	6	0.2424307796109133
1101	7	0.21637853056213174
1101	8	0.189346584339539
1101	9	0.2785972085254471
1101	10-11	0.2745512651484461
1101	12-13	0.3051388555114869
1101	14-15	0.407993848568438
1101	16-17	0.29467389626684337
1101	18-19	0.3335258415000837
1101	20-21	0.3491707266290973
1101	22-23	0.3284990658690248
1101	24-25	0.30570904802308974
1101	26-27	0.3229969412697997
1101	28-29	0.3805074976198881
1101	30-31	0.37334987949255094
1101	32-33	0.39223349582255906
1101	34-35	0.41486995688011064
1101	36-37	0.4240758060834864
1101	38-39	0.40244344963060996
1101	40-41	0.4211728836519555
1101	42-43	0.3711843658304659
1101	44-45	0.4071715014955899
1101	46-47	0.45032326557426217
1101	48-49	0.40797095058962185
1101	50-51	0.42808225911179676
1101	52-53	0.4009193138547005
1101	54-55	0.3440723606861411
1101	56-57	0.3857925318316333
1101	58-59	0.41902488670226035
1101	60-61	0.32855544527773617
1101	62-63	0.37125252435109957
1101	64-65	0.32220150566364225
1101	66-67	0.2830490008751809
1101	68-69	0.27852053857492365
1101	70-71	0.36371344213659995
1101	72-73	0.3788633407395636
1101	74-75	0.33637385426487043
1101	76-77	0.3170064673768067
1101	78-79	0.34408432248264376
1101	80-81	0.36685454446858756
1101	82-83	0.3521995831121316
1101	84-85	0.2827429955328924
1101	86-87	0.357607649607786
1101	88-89	0.4876917269744183
1101	90-91	0.4514556684564184
1101	92-93	0.4509352771613315
1101	94-95	0.5205815747586193
1101	96-97	0.5630883517030547
1101	98-99	0.5472916819580789
1101	100	0.5466698056753678
1102	1	0.043426859647876626
1102	2	0.08205894653112367
1102	3	0.11042246736067085
1102	4	0.09349120228307584
1102	5	-0.019522875798720918
1102	6	0.003676096357153824
1102	7	-0.03539972273413383
1102	8	0.02054706213431956
1102	9	0.09358565362462912
1102	10-11	0.12668946194636987
1102	12-13	0.12809934273068535
1102	14-15	0.10907873343490593
1102	16-17	0.07295947739550712
1102	18-19	0.0510662680683609
1102	20-21	0.05099265570071765
1102	22-23	0.04740087602465337
1102	24-25	0.01875481191916606
1102	26-27	0.07422844158067932
1102	28-29	0.09255191706289168
1102	30-31	0.12925177628424933
1102	32-33	0.1270110483087592
1102	34-35	0.1314594940614029
1102	36-37	0.09430551423891842
1102	38-39	0.080206476370698
1102	40-41	0.1151394601193445
1102	42-43	0.06061432523760146
1102	44-45	0.025662009886431747
1102	46-47	0.0868763865592328
1102	48-49	0.10827324178445963
1102	50-51	0.05422596335734653
1102	52-53	-0.0021191012052170777
1102	54-55	0.03571463926172669
1102	56-57	-0.06601140413814477
1102	58-59	-0.04471416061683442
1102	60-61	-0.08980133327981576
1102	62-63	0.01696608224542473
1102	64-65	0.011679777257612045
1102	66-67	-0.07328456388910354
1102	68-69	-0.0899380568537893
1102	70-71	0.013174913557215007
1102	72-73	-0.009006375885377338
1102	74-75	-0.045227338408594164
1102	76-77	-0.061455566576178455
1102	78-79	-0.0972670203871786
1102	80-81	-0.08536634975882151
1102	82-83	-0.10170504749763865
1102	84-85	-0.10020757352020127
1102	86-87	-0.11403063506589461
1102	88-89	-0.0589700709289005
1102	90-91	-0.05451774028926337
1102	92-93	-0.03769012494730006
1102	94-95	-0.04784681138553282
1102	96-97	-0.06836778044168668
1102	98-99	-0.04703217800713588
1102	100	-0.021998884010994146
1103	1	0.023209935862155362
1103	2	-0.042406374987926654
1103	3	0.10656354871375129
1103	4	0.06756710124786025
1103	5	0.030522053553738715
1103	6	0.0028014583813202876
1103	7	0.06278603045891629
1103	8	-4.1673697400312903E-4
1103	9	0.04723169104829594
1103	10-11	0.08777581521274413
1103	12-13	0.09297657049558694
1103	14-15	0.09809710682823436
1103	16-17	0.08832777594198404
1103	18-19	0.12816474223829033
1103	20-21	0.1189241338042919
1103	22-23	0.14849097685848278
1103	24-25	0.10360250729024756
1103	26-27	0.13745545142099758
1103	28-29	0.17218332266484282
1103	30-31	0.1254511371420719
1103	32-33	0.11350599794941019
1103	34-35	0.12590678662208177
1103	36-37	0.13305874672141016
1103	38-39	0.12104117112947677
1103	40-41	0.11316621407804206
1103	42-43	0.09026493977802375
1103	44-45	0.07460204411339078
1103	46-47	0.06983060788834905
1103	48-49	0.075978824279197
1103	50-51	0.14102461739886252
1103	52-53	0.06843380683757516
1103	54-55	0.08824208364924857
1103	56-57	0.05595156470136686
1103	58-59	0.05233306938845317
1103	60-61	0.08907146914859254
1103	62-63	0.08767870217023699
1103	64-65	0.10070997786142755
1103	66-67	0.09209574516905406
1103	68-69	0.020209607513685057
1103	70-71	0.09108039269748502
1103	72-73	0.13110920254829495
1103	74-75	0.1431232327185583
1103	76-77	0.046311254966873605
1103	78-79	0.060101604127083874
1103	80-81	0.03568701682820574
1103	82-83	0.0797462033069607
1103	84-85	0.09706858427273346
1103	86-87	0.1023848409966277
1103	88-89	0.15070615644312113
1103	90-91	0.1756219424242289
1103	92-93	0.24438310624831772
1103	94-95	0.19903032709283863
1103	96-97	0.2296363126913583
1103	98-99	0.25534541336226013
1103	100	0.2960090067118877
1104	1	0.202345130930361
1104	2	0.14578429365893797
1104	3	0.14236276947077897
1104	4	0.20108878031255273
1104	5	0.09408800599837974
1104	6	0.06335359287464826
1104	7	0.06014680914370274
1104	8	0.12595810277908015
1104	9	0.08357257411725527
1104	10-11	0.08991078183424861
1104	12-13	0.12124706895144755
1104	14-15	0.06266431808801798
1104	16-17	0.0761168396523928
1104	18-19	0.11679954340427656
1104	20-21	0.09826616697859691
1104	22-23	0.06512567627574128
1104	24-25	0.08716727505187549
1104	26-27	0.13537942619448273
1104	28-29	0.18827336390788219
1104	30-31	0.18631793476142633
1104	32-33	0.17818728292776598
1104	34-35	0.148226725133064
1104	36-37	0.16176162845143693
1104	38-39	0.08017164047700476
1104	40-41	0.12298885284981509
1104	42-43	0.1142477607684853
1104	44-45	0.1294108568668264
1104	46-47	0.07313636790364342
1104	48-49	0.029721360524170848
1104	50-51	0.08913831547989304
1104	52-53	-0.008972411369001065
1104	54-55	-0.019838397767927063
1104	56-57	0.006084135673951607
1104	58-59	0.05808515752235621
1104	60-61	0.04236882220829585
1104	62-63	0.029556566556955488
1104	64-65	-0.05452557124223745
1104	66-67	-6.903225164194282E-4
1104	68-69	-0.08032094310921423
1104	70-71	-0.06695581599031897
1104	72-73	-0.002815031281066638
1104	74-75	-0.02212064332092467
1104	76-77	-0.1003519480796271
1104	78-79	-0.05681572773648469
1104	80-81	0.03943880576622405
1104	82-83	-0.02119742500626387
1104	84-85	0.020490456884445507
1104	86-87	-0.03983108416809955
1104	88-89	0.01713301750709917
1104	90-91	0.02960275218596564
1104	92-93	0.10067578813741207
1104	94-95	0.027318332314035132
1104	96-97	0.02135134496876745
1104	98-99	0.12987455309991702
1104	100	0.1931232236528473
1105	1	0.06368869316330361
1105	2	0.0094228197148567
1105	3	0.05537260671012234
1105	4	0.04614685599629098
1105	5	0.01492546455468613
1105	6	0.007436111621700547
1105	7	0.025383412030734576
1105	8	-0.030382100159897618
1105	9	-0.019224628876287397
1105	10-11	-0.08371965427688366
1105	12-13	-0.009913543483548892
1105	14-15	0.027896007674215184
1105	16-17	0.029850126279733047
1105	18-19	0.01127405465016551
1105	20-21	0.012063738922037714
1105	22-23	0.1456372385516147
1105	24-25	0.03612472408855183
1105	26-27	0.07857689568545112
1105	28-29	0.13643231577196957
1105	30-31	0.08915638140592819
1105	32-33	0.09249792826003755
1105	34-35	0.10139133228440755
1105	36-37	0.06830312854550158
1105	38-39	0.038861324601057845
1105	40-41	0.021952233722380754
1105	42-43	0.023666224666001767
1105	44-45	0.04783120538175467
1105	46-47	0.05553405434103098
1105	48-49	0.019929229960020223
1105	50-51	0.04688399073791061
1105	52-53	0.022368707939577348
1105	54-55	-0.023042696591076606
1105	56-57	-0.012029956661727681
1105	58-59	-0.017159970175569583
1105	60-61	0.019630892652052978
1105	62-63	0.07516923380142515
1105	64-65	0.08717329158285736
1105	66-67	0.021020165646376654
1105	68-69	-0.018843055910934936
1105	70-71	0.029786921363374574
1105	72-73	0.02874648466471541
1105	74-75	0.004892513063701642
1105	76-77	0.0479649125937911
1105	78-79	0.05949759149208944
1105	80-81	0.029195103861397342
1105	82-83	0.04021387526744746
1105	84-85	0.04769800840632854
1105	86-87	0.04862073099707942
1105	88-89	0.1163264625211653
1105	90-91	0.10153537925804557
1105	92-93	0.09113495341345512
1105	94-95	0.09958891649427315
1105	96-97	0.10557976472986752
1105	98-99	0.15517791199145847
1105	100	0.236765095744623
1106	1	0.17364245711812032
1106	2	0.1272749537743998
1106	3	0.16360538626330623
1106	4	0.20956757880792765
1106	5	0.17565203623252046
1106	6	0.16996583751723904
1106	7	0.14018338655387197
1106	8	0.16267392340758846
1106	9	0.20697597356772945
1106	10-11	0.10029004827229926
1106	12-13	0.1670180251861666
1106	14-15	0.18116601518796216
1106	16-17	0.16219733541026216
1106	18-19	0.16245075143500998
1106	20-21	0.1979211621133814
1106	22-23	0.15887727970404342
1106	24-25	0.24132323698970026
1106	26-27	0.217355927576115
1106	28-29	0.2673526457449782
1106	30-31	0.22199029447454421
1106	32-33	0.22118197925849614
1106	34-35	0.26761838340171806
1106	36-37	0.25515412527953885
1106	38-39	0.21163205914886873
1106	40-41	0.15198863531261253
1106	42-43	0.21811722102626874
1106	44-45	0.23135563578532725
1106	46-47	0.19647022476159037
1106	48-49	0.2197978426547067
1106	50-51	0.1516914404234413
1106	52-53	0.10171415512137116
1106	54-55	0.16055418251129083
1106	56-57	0.1476060563731849
1106	58-59	0.20450453287683956
1106	60-61	0.1662778200751518
1106	62-63	0.21418178350760542
1106	64-65	0.13853423443659096
1106	66-67	0.15725032058833222
1106	68-69	0.1153582491061087
1106	70-71	0.13677899999415644
1106	72-73	0.18534052649699362
1106	74-75	0.22973755468651547
1106	76-77	0.19988478502620666
1106	78-79	0.22800622651188007
1106	80-81	0.173368300833026
1106	82-83	0.16970287999053113
1106	84-85	0.13234527759075831
1106	86-87	0.18994970991292348
1106	88-89	0.16672632598542947
1106	90-91	0.1496086854648908
1106	92-93	0.2134360886681428
1106	94-95	0.22347509122377218
1106	96-97	0.23103580193404483
1106	98-99	0.2540809509754922
1106	100	0.26912209833562173
1107	1	0.12245732084452499
1107	2	0.1308008134091665
1107	3	0.13075081017121448
1107	4	0.044316928472042605
1107	5	0.08678899754360714
1107	6	0.05274030812272912
1107	7	0.08310320579841601
1107	8	0.056497164600152416
1107	9	0.04343005384260579
1107	10-11	0.05407304746174901
1107	12-13	-0.011039365641735799
1107	14-15	-0.003779823873323096
1107	16-17	0.0019256830357363697
1107	18-19	0.046572331361467434
1107	20-21	-0.005511604895609423
1107	22-23	-0.016245481754772584
1107	24-25	-0.012907155004356241
1107	26-27	0.034520531262430154
1107	28-29	0.034657352455347734
1107	30-31	0.03347254980764802
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2216	30-31	-0.3124514559184206
2216	32-33	-0.24797938668128694
2216	34-35	-0.26615137352325746
2216	36-37	-0.23830383583463544
2216	38-39	-0.25794203610363553
2216	40-41	-0.2596489097500907
2216	42-43	-0.28811088040530564
2216	44-45	-0.23157278536805137
2216	46-47	-0.1352426310721384
2216	48-49	-0.14734703464336718
2216	50-51	-0.16606880460552986
2216	52-53	-0.13091634158945453
2216	54-55	-0.1104609831523291
2216	56-57	-0.18996791297725935
2216	58-59	-0.20710900927410592
2216	60-61	-0.12897222258411034
2216	62-63	-0.13067740922264193
2216	64-65	-0.08426957336118335
2216	66-67	-0.08179206085477375
2216	68-69	-0.11980894526398345
2216	70-71	-0.13829078331389155
2216	72-73	-0.031958902882841755
2216	74-75	-0.050442181833979305
2216	76-77	-0.05530276250471644
2216	78-79	-0.09281988728738355
2216	80-81	-0.06511528326363702
2216	82-83	-0.07377663090762354
2216	84-85	-0.09870598352071625
2216	86-87	-0.05992489122736799
2216	88-89	-0.04133040985306735
2216	90-91	-0.06987870744602276
2216	92-93	-0.03856308466554026
2216	94-95	-0.02798288438272678
2216	96-97	-0.0394137479459431
2216	98-99	-0.05745252611700025
2216	100	0.018191381658496653
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32741.0
3	5771.0
4	3805.0
5	3166.0
6	2751.0
7	3243.0
8	4008.0
9	4409.0
10	4844.0
11	5401.0
12	6105.0
13	6611.0
14	7230.0
15	7542.0
16	7566.0
17	7586.0
18	7746.0
19	7708.0
20	7775.0
21	8360.0
22	9733.0
23	12352.0
24	18309.0
25	25059.0
26	26040.0
27	24061.0
28	25797.0
29	32590.0
30	40517.0
31	50183.0
32	66242.0
33	90027.0
34	126982.0
35	193613.0
36	361049.0
37	782862.0
38	1279632.0
39	527337.0
40	976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.463203666914765	19.37746838467678	13.043345272818677	24.115982675589777
2	15.491485461357113	20.016502894267475	40.16140801045166	24.330603633923754
3	18.46441340152855	25.163743617127793	29.644061008577243	26.72778197276641
4	12.208437254967384	16.494214102200498	38.92864441701006	32.36870422582206
5	13.066873961505515	38.009093653368204	35.69420570258926	13.229826682537027
6	31.373812657172266	37.99416263827902	17.75475262876725	12.877272075781466
7	27.745787503215023	32.06915215334345	22.610868516627313	17.574191826814207
8	25.26680069030688	36.50331210742184	20.234320719578726	17.995566482692553
9	25.14423200966785	16.675401682596018	20.746605397327684	37.433760910408445
10-11	24.055990257804606	26.727470684475236	28.971980763203796	20.244558294516366
12-13	24.75737384280298	24.00782877295581	28.78738066431204	22.447416719929166
14-15	21.957559539981848	25.9456779312605	25.797711637083182	26.29905089167447
16-17	21.872798974300032	29.21909220757576	26.380199893692698	22.52790892443151
18-19	21.84497199041801	28.516750302092227	27.996785501553887	21.641492205935876
20-21	22.977918658553687	27.763451655462056	27.479596352598794	21.77903333338546
22-23	22.390787768730476	27.60475575927337	27.513893246907312	22.49056322508884
24-25	22.441321418625378	27.75229844995941	27.49089370865419	22.315486422761015
26-27	22.16531554018932	27.721112170537086	27.566381247143752	22.547191042129842
28-29	22.176704855385605	27.75416556228738	27.69837974853772	22.37074983378929
30-31	22.44842031850082	28.29118555862742	27.163930657893093	22.096463464978672
32-33	22.05864760068401	27.82840615609804	27.7767743324417	22.336171910776248
34-35	22.228531860648086	27.599713642605227	27.892325658305367	22.27942883844132
36-37	22.466749292873594	27.886918148457195	27.303731052738833	22.342601505930375
38-39	22.340261406772267	28.089735377373	27.451594089290264	22.118409126564472
40-41	22.130091919510686	27.90203938677936	27.727675582734985	22.240193110974964
42-43	22.096736848976672	27.758276056210303	27.63833840554718	22.506648689265848
44-45	22.187317259272156	27.743703816434234	27.674633684647226	22.394345239646384
46-47	22.309953239464807	27.913073978794106	27.569236550352528	22.207736231388555
48-49	22.257593366618373	27.80596264292936	27.524103857659554	22.412340132792714
50-51	22.17039702950353	27.757068258200125	27.620845801390654	22.45168891090569
52-53	22.266659978201684	27.68296154805825	27.59011349326148	22.460264980478588
54-55	22.27029607459218	28.09451393496063	27.398547639236252	22.236642351210932
56-57	22.15882218820234	27.797088042734764	27.529044656525848	22.515045112537052
58-59	22.14505493245557	27.424287749027915	27.82203406171843	22.60862325679809
60-61	22.21491440934074	27.860634159783565	27.59621091060227	22.328240520273425
62-63	22.144433918543598	27.832863229027026	27.526371464006306	22.49633138842307
64-65	22.091812332229402	27.656008172723705	27.624986531448272	22.627192963598624
66-67	21.985915120445092	27.66597575111823	27.490647624862174	22.85746150357451
68-69	22.316400080571253	27.75333105586647	27.504881309840506	22.42538755372177
70-71	22.274077122829535	27.59519771504898	27.576592489403946	22.554132672717532
72-73	22.123057195482293	27.658860379432284	27.633714812003007	22.584367613082417
74-75	22.115747668945584	27.772204330733384	27.531457476090132	22.5805905242309
76-77	22.156353181861178	27.676979326299563	27.46115326295393	22.70551422888533
78-79	22.262612143920496	27.784109875179507	27.49070966655905	22.46256831434095
80-81	22.289560605000517	27.38155404305861	27.716817106389996	22.61206824555088
82-83	22.080946982025072	27.552602222782173	27.83244993059506	22.534000864597694
84-85	22.0657440316469	27.467446738459017	27.603664846907392	22.863144382986693
86-87	22.173242282239368	27.60133837968296	27.59882380393814	22.626595534139536
88-89	22.057878681673802	27.45237177136353	27.603558974281007	22.88619057268166
90-91	22.031283138779344	27.419647021288252	27.692268166506796	22.85680167342561
92-93	22.203666288906838	27.579888601483827	27.565352319369328	22.651092790240007
94-95	22.010316729817614	27.313960926752113	27.76342896340836	22.912293380021918
96-97	21.99108527959775	27.31965619704911	27.753308814422915	22.935949708930224
98-99	21.91832363410762	27.45139051066862	27.619675549694872	23.010610305528886
100	22.11434246542668	27.14879324629969	27.57627282575561	23.160591462518017
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	249.0
1	240.5
2	223.0
3	190.5
4	142.0
5	113.0
6	104.5
7	112.0
8	132.0
9	147.0
10	156.5
11	168.0
12	180.0
13	199.5
14	228.5
15	283.5
16	395.0
17	534.5
18	743.0
19	1066.0
20	1546.0
21	2344.5
22	3767.0
23	5898.5
24	8725.0
25	12697.0
26	17886.5
27	24756.0
28	33628.5
29	43239.5
30	53055.5
31	63489.0
32	74363.5
33	85681.0
34	96557.0
35	105820.0
36	114363.5
37	123000.5
38	130088.0
39	133943.5
40	136281.5
41	138517.5
42	140157.0
43	140685.5
44	138757.5
45	137807.5
46	137066.0
47	134030.5
48	133584.5
49	132349.0
50	128022.5
51	122752.0
52	115514.5
53	110787.5
54	108314.0
55	103599.0
56	95937.5
57	88187.5
58	79045.5
59	68487.0
60	59460.5
61	50383.0
62	43245.5
63	36203.5
64	27994.0
65	21683.5
66	16655.5
67	12705.0
68	10138.0
69	8074.5
70	6320.0
71	4606.5
72	3259.0
73	2375.5
74	1683.5
75	1064.5
76	560.0
77	325.0
78	232.5
79	147.5
80	97.5
81	60.0
82	39.5
83	35.0
84	29.0
85	23.5
86	21.0
87	18.5
88	12.0
89	7.0
90	5.0
91	7.0
92	7.5
93	6.0
94	3.0
95	2.5
96	3.0
97	3.5
98	3.0
99	3.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32847551247104734
2	0.1162666775064107
3	0.06423069476766077
4	0.03921590086220262
5	0.04291600579405164
6	0.009875632177259
7	0.0072438674017889235
8	0.015503960805987083
9	0.017770926503669227
10-11	0.02114531797320759
12-13	0.01567333180638862
14-15	0.018409324889798108
16-17	0.019269208430298234
18-19	0.01924315135331338
20-21	0.0238422254411398
22-23	0.015972988191714423
24-25	0.024975708289980872
26-27	0.019425550892207346
28-29	0.01907378035291184
30-31	0.019620978969593736
32-33	0.024702108981639923
34-35	0.014748305573426369
36-37	0.009562947253440772
38-39	0.01279402479956245
40-41	0.009119976944698283
42-43	0.008299179019675437
44-45	0.008468550020076978
46-47	0.007947408480379933
48-49	0.004403646010440028
50-51	0.006670611708122174
52-53	0.007204781786311645
54-55	0.0029965638532580075
56-57	0.002709936006424633
58-59	0.0027881072373791894
60-61	0.0034265056235080697
62-63	0.002749021621901911
64-65	0.0022278800822048664
66-67	0.001758852696477526
68-69	0.0034395341620004952
70-71	0.0025405650060230933
72-73	0.001941252235371492
74-75	0.0022930227746669972
76-77	0.003856447393758132
78-79	0.003595876623909609
80-81	0.0037913047012960006
82-83	0.0027620501603943374
84-85	0.0034786197774777736
86-87	0.002696907467932207
88-89	0.597280318646783
90-91	0.6065566380533904
92-93	0.6028565331215414
94-95	0.5905445642461987
96-97	0.5883297127024862
98-99	0.585658862311539
100	0.5882645700100242
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3837729.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.78951158545763
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36692691624287	41.82049361430184
2	15.529032286090688	17.94830382415967
3	5.49250595533018	9.522277096162455
4	2.511156732642073	5.80474084375676
5	1.3100705821320353	3.785416954194324
6	0.7964997214357153	2.7617597927833826
7	0.4660557665351614	1.8853194583757134
8	0.32907555565019375	1.5213692508590224
9	0.21811737286276064	1.1344406801437878
>10	0.9034291408881394	8.823770201955265
>50	0.050074074011984285	1.9973694423940744
>100	0.02610677355716382	2.5734959775246997
>500	7.683373760521204E-4	0.29072758195964266
>1k	1.807852449748215E-4	0.13051528142933377
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	2.605707698485224E-5	0.0
5	0.0	0.0	0.0	2.605707698485224E-5	0.0
6	0.0	0.0	0.0	2.605707698485224E-5	0.0
7	0.0	0.0	0.0	2.605707698485224E-5	0.0
8	0.0	0.0	0.0	2.605707698485224E-5	0.0
9	0.0	0.0	0.0	5.211415396970448E-5	0.0
10-11	0.0	0.0	0.0	9.119976944698283E-5	0.0
12-13	0.0	0.0	0.0	1.0422830793940896E-4	0.0
14-15	0.0	0.0	0.0	1.0422830793940896E-4	0.0
16-17	0.0	0.0	0.0	1.0422830793940896E-4	0.0
18-19	0.0	0.0	0.0	1.1725684643183508E-4	0.0
20-21	0.0	0.0	0.0	1.6937100040153956E-4	0.0
22-23	0.0	0.0	0.0	2.2148515437124403E-4	0.0
24-25	0.0	0.0	0.0	3.126849238182269E-4	0.0
26-27	0.0	0.0	0.0	3.908561547727836E-4	0.0
28-29	0.0	0.0	0.0	0.001094397233363794	0.0
30-31	0.0	0.0	0.0	0.004690273857273403	0.0
32-33	0.0	0.0	0.0	0.012077455182479012	0.0
34-35	0.0	0.0	0.0	0.02342531220938216	0.0
36-37	0.0	0.0	0.0	0.04352834710319567	0.0
38-39	0.0	0.0	0.0	0.07883568641767044	0.0
40-41	0.0	0.0	0.0	0.11921112720569899	0.0
42-43	2.605707698485224E-5	0.0	0.0	0.16142359192115963	0.0
44-45	2.605707698485224E-5	0.0	0.0	0.21007215465187876	0.0
46-47	2.605707698485224E-5	0.0	0.0	0.2705897159491981	0.0
48-49	2.605707698485224E-5	0.0	0.0	0.3373349186458971	0.0
50-51	2.605707698485224E-5	0.0	0.0	0.41158455951423356	0.0
52-53	5.211415396970448E-5	0.0	0.0	0.48412746184006217	0.0
54-55	7.817123095455672E-5	0.0	0.0	0.5649044004931041	0.0
56-57	7.817123095455672E-5	0.0	0.0	0.6632177519569515	0.0
58-59	7.817123095455672E-5	0.0	0.0	0.7726444467548386	0.0
60-61	7.817123095455672E-5	0.0	0.0	0.8880251836437643	0.0
62-63	7.817123095455672E-5	0.0	0.0	1.006923625925645	0.0
64-65	7.817123095455672E-5	0.0	0.0	1.1317500532215798	0.0
66-67	7.817123095455672E-5	0.0	0.0	1.2750770051767595	0.0
68-69	7.817123095455672E-5	0.0	0.0	1.4387675627956011	0.0
70-71	7.817123095455672E-5	0.0	0.0	1.6130763792857703	0.0
72-73	7.817123095455672E-5	0.0	0.0	1.7989545379572136	0.0
74-75	7.817123095455672E-5	0.0	0.0	1.9842985265504678	0.0
76-77	7.817123095455672E-5	0.0	0.0	2.1893937794982397	0.0
78-79	7.817123095455672E-5	0.0	0.0	2.4244807280555767	0.0
80-81	7.817123095455672E-5	0.0	0.0	2.680673908970644	0.0
82-83	9.119976944698283E-5	1.302853849242612E-5	0.0	2.943837357979159	0.0
84-85	1.0422830793940896E-4	2.605707698485224E-5	0.0	3.2082254896059625	0.0
86-87	1.0422830793940896E-4	2.605707698485224E-5	0.0	3.487023184805389	0.0
88	1.0422830793940896E-4	2.605707698485224E-5	0.0	3.721419620822627	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	10885	0.0	26.52954	1
TACATGG	10980	0.0	25.221848	2
GTATCAA	6505	0.0	24.694756	1
ACATGGG	10640	0.0	24.596727	3
TCAACGC	6295	0.0	24.103493	4
ATCAACG	6335	0.0	24.030462	3
CAACGCA	6455	0.0	23.505426	5
AACGCAG	6575	0.0	22.99929	6
TCTAACG	175	4.454603E-6	21.479082	3
TATCAAC	7555	0.0	20.786242	2
GTCGTAC	260	1.6418198E-8	19.930445	1
GGTATCA	4865	0.0	19.463049	1
ACGCAGA	7855	0.0	18.713388	7
TAGACCG	180	1.344115E-4	18.267849	5
CATGGGG	7065	0.0	17.886003	4
CGCAGAG	8065	0.0	17.876741	8
GTATACG	300	8.456482E-8	17.273052	1
TATACTG	1945	0.0	17.147491	5
GAGTACA	7530	0.0	17.141674	1
GTATTAG	1805	0.0	16.44224	1
>>END_MODULE