FastQCFastQC Report
Wed 25 May 2016
SRR1033155_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033155_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3837729
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44870.116918104431032No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39090.1018571139337874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA101050.034.7773861
GGTATCA64400.034.4801251
TCAACGC112800.030.0460784
ATCAACG114300.029.651773
CAACGCA115400.029.409865
AACGCAG119600.028.5283416
GTACATG108200.028.3921681
TATCAAC123400.027.8464282
TACATGG111650.026.3983082
ACATGGG107150.026.2343673
ACGCAGA138600.024.3459457
CGCAGAG139100.024.1558158
GCAGAGT153350.021.6659839
AGAGTAC124500.020.49619710-11
CGCGCGT1909.056064E-619.7879967
GAGTACT85750.019.53746212-13
CATGGGG67550.018.7888434
GAGTACA78650.018.4231821
CATGGGA44900.018.2164824
AGTACTT93650.018.0398912-13
Produced by FastQC (version 0.11.5)