FastQCFastQC Report
Wed 25 May 2016
SRR1033154_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033154_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2640374
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC28500.036.7567564
ATCAACG30050.034.8674283
GGTATCA19050.034.365751
CAACGCA30850.034.1090855
GTATCAA32050.034.093061
AACGCAG31700.033.7763636
GTACATG66750.028.7882271
TATCAAC38950.027.520122
TACATGG67350.027.0144622
ACGCAGA38500.026.9573447
CGCAGAG40300.025.4037028
ACATGGG68600.025.1363073
GAGTACA49750.019.9753951
CGCTACG1456.510701E-419.4889871
CATGGGG43950.019.3462544
TATACCG1708.666561E-519.3431115
GCAGAGT50950.019.1718799
GTCTCGC5400.018.3160381
GTATAGG11800.017.5621091
CTGTCGC7000.016.7721029
Produced by FastQC (version 0.11.5)