##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033154_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2640374
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.415654751940444	33.0	31.0	34.0	30.0	34.0
2	31.603467160334105	33.0	31.0	34.0	30.0	34.0
3	31.73107559762367	34.0	31.0	34.0	30.0	34.0
4	35.165609493200584	37.0	35.0	37.0	33.0	37.0
5	35.25746807081118	37.0	35.0	37.0	33.0	37.0
6	35.42598169804732	37.0	36.0	37.0	35.0	37.0
7	35.38742769016814	37.0	36.0	37.0	33.0	37.0
8	35.398340538120735	37.0	36.0	37.0	33.0	37.0
9	37.07780223559238	39.0	38.0	39.0	35.0	39.0
10-11	37.059716161422585	39.0	38.0	39.0	34.5	39.0
12-13	37.012151498234715	39.0	38.0	39.0	34.0	39.0
14-15	38.46392651192596	41.0	39.0	41.0	34.5	41.0
16-17	38.47111318320813	41.0	39.0	41.0	34.5	41.0
18-19	38.45812127372865	41.0	39.0	41.0	34.0	41.0
20-21	38.48272081909609	41.0	39.0	41.0	34.0	41.0
22-23	38.444426622895094	41.0	39.0	41.0	34.0	41.0
24-25	38.43200982133592	41.0	39.0	41.0	34.0	41.0
26-27	38.38307982126774	40.0	39.0	41.0	34.0	41.0
28-29	38.35951478843528	40.0	39.0	41.0	34.0	41.0
30-31	38.36124655067805	40.0	39.0	41.0	34.0	41.0
32-33	38.43023942820221	41.0	39.0	41.0	34.0	41.0
34-35	38.40660868498175	41.0	39.0	41.0	34.0	41.0
36-37	38.33081658128735	41.0	39.0	41.0	34.0	41.0
38-39	38.25648639170057	41.0	39.0	41.0	33.5	41.0
40-41	38.19739646731864	41.0	38.0	41.0	33.0	41.0
42-43	38.08944130642098	40.0	38.0	41.0	33.0	41.0
44-45	37.97045399628992	40.0	38.0	41.0	33.0	41.0
46-47	37.85138260716096	40.0	38.0	41.0	33.0	41.0
48-49	37.73862736869853	40.0	38.0	41.0	33.0	41.0
50-51	37.62279396782425	40.0	38.0	41.0	32.5	41.0
52-53	37.4820239481225	40.0	37.0	41.0	32.0	41.0
54-55	37.336548155677946	40.0	37.0	41.0	32.0	41.0
56-57	37.18671919205386	40.0	37.0	41.0	31.5	41.0
58-59	36.99229559903256	40.0	36.0	41.0	31.0	41.0
60-61	36.77628794254147	39.5	36.0	41.0	31.0	41.0
62-63	36.52162326246206	39.0	35.0	41.0	31.0	41.0
64-65	36.261731103245225	39.0	35.0	41.0	30.5	41.0
66-67	35.97550555337994	38.5	35.0	40.5	30.0	41.0
68-69	35.67453285027045	38.0	35.0	40.0	30.0	41.0
70-71	35.33401177257464	37.0	35.0	40.0	29.5	41.0
72-73	34.972472081606625	37.0	34.5	39.0	29.0	41.0
74-75	34.60096827191906	36.0	34.0	39.0	29.0	41.0
76-77	34.26203465872638	36.0	34.0	39.0	29.0	40.0
78-79	33.88716181874234	35.0	34.0	37.5	29.0	39.5
80-81	33.518666105635035	35.0	34.0	37.0	28.0	39.0
82-83	33.28032543874467	35.0	34.0	37.0	29.0	39.0
84-85	32.99307389786447	35.0	34.0	36.0	28.0	37.5
86-87	32.724027164333535	35.0	34.0	36.0	28.0	37.0
88-89	32.341171743093966	35.0	34.0	35.5	27.0	37.0
90-91	32.135671120833635	35.0	34.0	35.0	27.0	36.5
92-93	31.944578305952113	35.0	33.0	35.0	26.0	36.0
94-95	31.763165937855774	35.0	33.0	35.0	26.0	36.0
96-97	31.57698966131313	35.0	33.0	35.0	25.5	36.0
98-99	31.375642049194546	35.0	33.0	35.0	25.0	35.5
100	31.204471412004512	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	-0.044750195850948415
1101	2	-0.02164055188032421
1101	3	0.01325681555523417
1101	4	0.001816277397409749
1101	5	-0.06386655195564117
1101	6	0.07518023555385156
1101	7	0.0531974100129986
1101	8	0.07501352111781756
1101	9	0.09274903045701421
1101	10-11	0.08695425332339113
1101	12-13	0.032574328101766525
1101	14-15	0.043313479969199875
1101	16-17	-0.020586362159683347
1101	18-19	0.023116748799829168
1101	20-21	-0.011208622596790008
1101	22-23	0.05453494841511031
1101	24-25	0.09383982731751672
1101	26-27	0.047121103016174004
1101	28-29	0.06786245526755863
1101	30-31	0.09799377704456447
1101	32-33	0.11637254591270363
1101	34-35	0.1561247591618553
1101	36-37	0.15098831576353433
1101	38-39	0.12132783973179073
1101	40-41	0.10606289863817153
1101	42-43	0.11932408629292013
1101	44-45	0.11420904188766201
1101	46-47	0.17679590556750924
1101	48-49	0.20072820667222402
1101	50-51	0.1525565136004161
1101	52-53	0.07928026288459478
1101	54-55	0.11513954228028922
1101	56-57	0.19878175778038099
1101	58-59	0.18340308278717998
1101	60-61	0.10503586670265719
1101	62-63	0.15478147946565457
1101	64-65	0.11406559295674867
1101	66-67	0.10720027114685138
1101	68-69	0.10698900939613765
1101	70-71	0.1800649110964514
1101	72-73	0.18530291909746666
1101	74-75	0.084672397754332
1101	76-77	0.07055403924064763
1101	78-79	0.12448096200250092
1101	80-81	0.12761031729977645
1101	82-83	0.12792108648024225
1101	84-85	-0.0017285151648138708
1101	86-87	0.0077208766825691555
1101	88-89	0.15521280729563358
1101	90-91	0.19169806542181078
1101	92-93	0.2729614749450704
1101	94-95	0.25333833767102476
1101	96-97	0.3121327010698245
1101	98-99	0.33891477920712276
1101	100	0.3951511258831282
1102	1	0.12169508188624789
1102	2	0.12316273308815795
1102	3	0.1869434786851052
1102	4	0.17757240313184752
1102	5	0.04255398328847093
1102	6	0.11807330417326511
1102	7	0.1468480931075291
1102	8	0.2427659508556701
1102	9	0.32983720144374473
1102	10-11	0.27484915592055614
1102	12-13	0.25060183188843865
1102	14-15	0.2719567600651658
1102	16-17	0.2310151082186067
1102	18-19	0.26071945871490954
1102	20-21	0.281679018714847
1102	22-23	0.2680643824388653
1102	24-25	0.23797114374267636
1102	26-27	0.23145836768019734
1102	28-29	0.23331336730401375
1102	30-31	0.23751855293068047
1102	32-33	0.24544967896149927
1102	34-35	0.3244582500378712
1102	36-37	0.25839032685242813
1102	38-39	0.2725146562584797
1102	40-41	0.3058893604722144
1102	42-43	0.28648297962265445
1102	44-45	0.23706350275021038
1102	46-47	0.30687773127765894
1102	48-49	0.232317889239539
1102	50-51	0.2681013331371531
1102	52-53	0.25259884901664265
1102	54-55	0.3488296909478166
1102	56-57	0.28321427169576907
1102	58-59	0.2580778249862874
1102	60-61	0.23190152259356012
1102	62-63	0.2954564677027207
1102	64-65	0.30937348186519387
1102	66-67	0.2668029575435966
1102	68-69	0.24790679427665907
1102	70-71	0.3014604814786068
1102	72-73	0.394704810182553
1102	74-75	0.3462538956400181
1102	76-77	0.27966884835846173
1102	78-79	0.27354909325832466
1102	80-81	0.2652035746538175
1102	82-83	0.21537035434396046
1102	84-85	0.21682291226989747
1102	86-87	0.2565055743280027
1102	88-89	0.3312381501092716
1102	90-91	0.3511610740227411
1102	92-93	0.416674172568797
1102	94-95	0.4322881325112533
1102	96-97	0.38010000696781887
1102	98-99	0.42695897799553606
1102	100	0.49044032826939343
1103	1	0.0917954822043221
1103	2	0.047198394463602966
1103	3	0.037664959905516326
1103	4	0.1500908535011476
1103	5	0.08059290956857268
1103	6	0.20926404213287242
1103	7	0.22939856253555035
1103	8	0.24411810214797214
1103	9	0.23051889472328213
1103	10-11	0.31061700511597934
1103	12-13	0.36486478532363265
1103	14-15	0.3605190407488408
1103	16-17	0.3020585016059343
1103	18-19	0.3218505597623391
1103	20-21	0.33629156023380347
1103	22-23	0.33743192111127485
1103	24-25	0.30359910951198543
1103	26-27	0.27114651209314644
1103	28-29	0.32631067519474044
1103	30-31	0.32124473351189664
1103	32-33	0.3043801047897219
1103	34-35	0.3141842953313869
1103	36-37	0.3744731322201602
1103	38-39	0.3525184652171447
1103	40-41	0.35898993120634515
1103	42-43	0.3160191024569272
1103	44-45	0.22478643352326344
1103	46-47	0.276612622529278
1103	48-49	0.31859835111062296
1103	50-51	0.2284944048968356
1103	52-53	0.2842410763168317
1103	54-55	0.2728215870398927
1103	56-57	0.2115360276721603
1103	58-59	0.251683375962898
1103	60-61	0.22018984566757638
1103	62-63	0.2983322024586812
1103	64-65	0.29172286324327246
1103	66-67	0.32601927300898126
1103	68-69	0.1469474708777767
1103	70-71	0.2452876313991581
1103	72-73	0.2800433241254652
1103	74-75	0.2917060948055905
1103	76-77	0.20298183821391547
1103	78-79	0.17161365162379383
1103	80-81	0.19784945675434784
1103	82-83	0.16393838040049502
1103	84-85	0.2502019608489192
1103	86-87	0.15105453933348656
1103	88-89	0.2353040683194294
1103	90-91	0.24981816932596956
1103	92-93	0.2800309741583433
1103	94-95	0.24028482638158977
1103	96-97	0.24656000666007927
1103	98-99	0.23926876594511626
1103	100	0.28044506333919017
1104	1	-0.0825188310652436
1104	2	-0.0835697967454756
1104	3	-0.020703195511277528
1104	4	-3.6926313008933676E-4
1104	5	-0.15194179540529262
1104	6	-0.05464045939928752
1104	7	-0.052671516319506395
1104	8	-0.016696336834193914
1104	9	0.0013173817476541672
1104	10-11	0.04271802642983147
1104	12-13	0.027261253232033766
1104	14-15	0.044881960054965475
1104	16-17	0.030616951726251784
1104	18-19	0.08311697560885278
1104	20-21	-0.03420790380366867
1104	22-23	0.006153087156981485
1104	24-25	0.05920111972242381
1104	26-27	0.1055546539020682
1104	28-29	0.08874427547178243
1104	30-31	0.03736330836067481
1104	32-33	0.0765456188663407
1104	34-35	0.027928546975537927
1104	36-37	0.04903869343368683
1104	38-39	0.06098445382515649
1104	40-41	0.023763700282863454
1104	42-43	-0.0060322634427549815
1104	44-45	-0.05710315947279554
1104	46-47	-0.04555406855196509
1104	48-49	-0.12684922191294845
1104	50-51	-0.05799397913112614
1104	52-53	-0.11423915546515673
1104	54-55	-0.12085387790175872
1104	56-57	-0.07346362277164786
1104	58-59	-0.08187193382400437
1104	60-61	-0.0016210000471232888
1104	62-63	0.04795141310266615
1104	64-65	-0.0987043880155909
1104	66-67	-0.06871659510069605
1104	68-69	-0.1000224158135552
1104	70-71	0.03246651298668013
1104	72-73	0.04947616672350108
1104	74-75	-0.01598301403032565
1104	76-77	0.00770783791980989
1104	78-79	0.004282308231843501
1104	80-81	0.008727929042059657
1104	82-83	0.11717063361701463
1104	84-85	0.05605384346781506
1104	86-87	0.13627692726598184
1104	88-89	0.19728252941850855
1104	90-91	0.17988183109510913
1104	92-93	0.10271494458914532
1104	94-95	0.15687270700609446
1104	96-97	0.16583237956804098
1104	98-99	0.20090742013818286
1104	100	0.20561323673721432
1105	1	0.06359767478242162
1105	2	0.12767953057024428
1105	3	0.11271535018112289
1105	4	0.10926957788179692
1105	5	0.14983361364472358
1105	6	0.17024568729465273
1105	7	0.15102976479874997
1105	8	0.09303342312750118
1105	9	0.16122103357923123
1105	10-11	0.1664747114962779
1105	12-13	0.22825808777933076
1105	14-15	0.23935697985183424
1105	16-17	0.2891540569569244
1105	18-19	0.20875946156347425
1105	20-21	0.31004623000746534
1105	22-23	0.31710145433312675
1105	24-25	0.31012456432973323
1105	26-27	0.33463193910179
1105	28-29	0.3322814591396508
1105	30-31	0.3550211257193041
1105	32-33	0.3336830633549752
1105	34-35	0.3161609543633688
1105	36-37	0.37538015718103424
1105	38-39	0.30707268817641875
1105	40-41	0.3241502251805386
1105	42-43	0.3495036638657396
1105	44-45	0.4212979288263554
1105	46-47	0.37872681575588985
1105	48-49	0.3481327545818118
1105	50-51	0.37982584856620605
1105	52-53	0.374690899693249
1105	54-55	0.39913884448989023
1105	56-57	0.36926500974050214
1105	58-59	0.3682533610828287
1105	60-61	0.30611052100031344
1105	62-63	0.39451511331976263
1105	64-65	0.3525006802554742
1105	66-67	0.36868894774954697
1105	68-69	0.3398422015731839
1105	70-71	0.3134426946772564
1105	72-73	0.2725490157878099
1105	74-75	0.39677438788395136
1105	76-77	0.3279967801156829
1105	78-79	0.4136689565828533
1105	80-81	0.32830496835819645
1105	82-83	0.29941766852020635
1105	84-85	0.26107361987403976
1105	86-87	0.24048013828047488
1105	88-89	0.28173965189171923
1105	90-91	0.38843257757856264
1105	92-93	0.34627862639055706
1105	94-95	0.3899441510213748
1105	96-97	0.35555420714124253
1105	98-99	0.4428970718838201
1105	100	0.4167005059698461
1106	1	0.059217023054639384
1106	2	0.0785156765979309
1106	3	0.03598108945680778
1106	4	0.05507045827747703
1106	5	0.016976618560072154
1106	6	0.05185826466769328
1106	7	0.05947819192474668
1106	8	0.09845627229429255
1106	9	0.08243162233586077
1106	10-11	0.044927365645335726
1106	12-13	0.049926050647933096
1106	14-15	0.10322691524606142
1106	16-17	0.10165787213257715
1106	18-19	0.12657840705531243
1106	20-21	0.10703042145203057
1106	22-23	0.07840229194775361
1106	24-25	0.13281908082087313
1106	26-27	0.16774121467176428
1106	28-29	0.18139499748998844
1106	30-31	0.12537398636673913
1106	32-33	0.17302904966248178
1106	34-35	0.15731498274189448
1106	36-37	0.19923052822682052
1106	38-39	0.1532181795008114
1106	40-41	0.15308924239128885
1106	42-43	0.21449388807674552
1106	44-45	0.19584958981655376
1106	46-47	0.23596077190467213
1106	48-49	0.2235513384141541
1106	50-51	0.20587273915016624
1106	52-53	0.13525903202588552
1106	54-55	0.2406359513416234
1106	56-57	0.22105300218549928
1106	58-59	0.15452077201204872
1106	60-61	0.15925773761416906
1106	62-63	0.27806030862483055
1106	64-65	0.2191320365935141
1106	66-67	0.2584244331612098
1106	68-69	0.2564525505005264
1106	70-71	0.25992053728641196
1106	72-73	0.20367248444973995
1106	74-75	0.24672356144347418
1106	76-77	0.15191093962131674
1106	78-79	0.16767811555851608
1106	80-81	0.2790077907581008
1106	82-83	0.21540469117628902
1106	84-85	0.2576041406407228
1106	86-87	0.30616762974023004
1106	88-89	0.29641055068963595
1106	90-91	0.2988368010258995
1106	92-93	0.33341666359601874
1106	94-95	0.275987951153418
1106	96-97	0.15622561420356718
1106	98-99	0.23108279782714192
1106	100	0.3048633089331041
1107	1	0.2700607889474398
1107	2	0.2677566641127811
1107	3	0.26522123632010874
1107	4	0.3049806781668494
1107	5	0.24395179942920464
1107	6	0.16790369721363874
1107	7	0.1942066135424696
1107	8	0.2718007110770344
1107	9	0.34520221319836963
1107	10-11	0.2606383073643386
1107	12-13	0.29264335354279325
1107	14-15	0.27861196192311866
1107	16-17	0.2509473863810072
1107	18-19	0.33086183395413826
1107	20-21	0.2603184997102943
1107	22-23	0.30231474816689285
1107	24-25	0.260479577055996
1107	26-27	0.35270483436985245
1107	28-29	0.31321825517206037
1107	30-31	0.2351237499642167
1107	32-33	0.22358078254766411
1107	34-35	0.3198390439344436
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2216	28-29	-0.36132066368625004
2216	30-31	-0.36813158043975136
2216	32-33	-0.28061807385772397
2216	34-35	-0.2772249767714783
2216	36-37	-0.2949687706857702
2216	38-39	-0.26853787874067336
2216	40-41	-0.3052778332848831
2216	42-43	-0.320057433974398
2216	44-45	-0.2760328374223988
2216	46-47	-0.17509598177434071
2216	48-49	-0.19006224783295522
2216	50-51	-0.2007028221445566
2216	52-53	-0.249100256859208
2216	54-55	-0.2583915891062887
2216	56-57	-0.2653132080126568
2216	58-59	-0.3102957993808957
2216	60-61	-0.25091773930898853
2216	62-63	-0.18549963514421108
2216	64-65	-0.13999054321732984
2216	66-67	-0.16215932715662973
2216	68-69	-0.16773457815060056
2216	70-71	-0.25086304466258724
2216	72-73	-0.1086947171978565
2216	74-75	-0.09352302864429873
2216	76-77	-0.19379835267463363
2216	78-79	-0.18336126369119654
2216	80-81	-0.18108851459660968
2216	82-83	-0.21974163654776646
2216	84-85	-0.2835839602083894
2216	86-87	-0.3845511020415273
2216	88-89	-0.29090579156925145
2216	90-91	-0.3301538980149985
2216	92-93	-0.2994796200798824
2216	94-95	-0.31032564191539436
2216	96-97	-0.28066003974501186
2216	98-99	-0.3175264212714666
2216	100	-0.36196500709694845
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	25070.0
3	4357.0
4	2815.0
5	2379.0
6	2033.0
7	2291.0
8	2478.0
9	2864.0
10	3264.0
11	3671.0
12	4057.0
13	4553.0
14	5236.0
15	5427.0
16	5195.0
17	5178.0
18	5207.0
19	5306.0
20	5415.0
21	5807.0
22	6858.0
23	8976.0
24	13287.0
25	18124.0
26	19130.0
27	17067.0
28	18256.0
29	22986.0
30	28333.0
31	34645.0
32	45559.0
33	62037.0
34	86704.0
35	131194.0
36	243998.0
37	522849.0
38	872351.0
39	384454.0
40	963.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.135862366140536	19.860446452795514	13.153660896203867	23.850030284860075
2	15.215276932509894	19.890445698537597	41.166321797370045	23.727955571582466
3	17.98934680489418	25.292602832135437	30.06630296989553	26.651747393074853
4	11.705080927398164	16.513743392763573	39.74007147996413	32.04110419987414
5	12.356919145724417	38.37276005762261	36.384727129986565	12.885593666666415
6	30.903994712351547	38.69229181304602	17.839791069707193	12.563922404895242
7	27.270864462662797	32.49294086232267	22.819526587998233	17.416668087016298
8	24.71620997128766	37.43418510746294	20.348601125766105	17.5010037954833
9	24.622374348274718	16.808034801107308	21.292533550309933	37.277057300308044
10-11	23.489176536412103	27.128312720053295	29.488376867795385	19.894133875739225
12-13	24.067365942554627	24.40138501626345	29.465270236127772	22.06597880505415
14-15	21.28727029467738	26.691898644799206	26.235044910724024	25.78578614979939
16-17	21.087949112925205	30.053833794622452	26.829488184047445	22.028728908404894
18-19	20.972626069933376	29.113419377518102	28.738061975450435	21.175892577098086
20-21	22.132603978933833	28.469539572937492	28.22785253681418	21.170003911314495
22-23	21.734682939006	28.155543543684598	28.33130238437322	21.778471132936183
24-25	21.67606211641771	28.31204283128005	28.226978054386148	21.784916997916092
26-27	21.36223091995049	28.22905055972817	28.396386602131336	22.012331918190007
28-29	21.405380650205462	28.298207056014157	28.40311617392148	21.893296119858903
30-31	21.658039684811012	28.801726141355477	28.061426358392143	21.478807815441368
32-33	21.306752856418225	28.410569609248128	28.591040766252345	21.691636768081306
34-35	21.404673913022894	28.14751248440574	28.668953162195287	21.778860440376086
36-37	21.810225270634785	28.572245629059566	28.011175941537847	21.6063531587678
38-39	21.56109637649704	28.708842510838846	28.189873494436828	21.540187618227286
40-41	21.313036736212037	28.383116546162015	28.574090760855363	21.72975595677059
42-43	21.356728056699424	28.289752773308365	28.506841231315583	21.846677938676628
44-45	21.452876123163804	28.527361685969606	28.38267252619887	21.637089664667723
46-47	21.448844725107925	28.637560613937158	28.37888433426225	21.534710326692668
48-49	21.477311032583792	28.502331200975657	28.305646770974178	21.714710995466373
50-51	21.43808497740434	28.36322889062822	28.316187347301998	21.88249878466544
52-53	21.45351832072056	28.38923861251884	28.367421918202545	21.789821148558055
54-55	21.56781314818739	28.793405889605918	28.15427288347081	21.484508078735885
56-57	21.44876932685377	28.487336491304216	28.226059660576837	21.837834521265172
58-59	21.24401323106471	28.10078697451787	28.68212209855579	21.97307769586163
60-61	21.668947674309607	28.595427644903637	28.13449177085541	21.60113290993135
62-63	21.391869763506442	28.56907224147821	28.246249627647114	21.79280836736823
64-65	21.412435658804043	28.233241557789334	28.41624978198653	21.938073001420094
66-67	21.261110106397584	28.309710992838973	28.300526543531923	22.128652357231523
68-69	21.705370131830264	28.475727467712815	28.176517797862278	21.642384602594642
70-71	21.58420300232036	28.283114442940633	28.289363674805877	21.84331887993313
72-73	21.3531088579875	28.407934676458606	28.390171775583738	21.848784689970152
74-75	21.48443314278832	28.3924378891809	28.2577383583284	21.86539060970238
76-77	21.436385806881432	28.401027239729732	28.168154167011483	21.994432786377352
78-79	21.553899804661793	28.480897476321275	28.219487045469393	21.745715673547533
80-81	21.45172777978409	28.27959821708405	28.314821733970412	21.95385226916145
82-83	21.305885850741294	28.3615780692834	28.503265708826408	21.829270371148898
84-85	21.316527926172053	28.279067424509552	28.327168000921105	22.077236648397285
86-87	21.522638849305057	28.417377834294548	28.19070055021792	21.869282766182476
88-89	21.284941179922132	28.30183898590684	28.30256291360323	22.110656920567795
90-91	21.28754777690792	28.163097565013846	28.396378243166335	22.1529764149119
92-93	21.449061869318808	28.362447033020377	28.248573147063066	21.93991795059775
94-95	21.183672023170455	28.085126729611748	28.47383456228888	22.25736668492892
96-97	21.22617841619066	28.078370967698923	28.463604281208315	22.2318463349021
98-99	21.24361964513074	28.174633712960794	28.337709449744636	22.24403719216383
100	21.36853699115705	28.110795362547208	28.194801667020343	22.3258659792754
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	250.0
1	247.0
2	243.5
3	217.5
4	183.5
5	163.0
6	148.5
7	147.5
8	153.0
9	160.5
10	156.0
11	156.0
12	171.0
13	212.0
14	275.0
15	327.0
16	421.5
17	575.0
18	812.0
19	1237.5
20	1908.5
21	2857.5
22	4217.5
23	6531.0
24	9517.0
25	12816.5
26	17657.5
27	23956.5
28	31281.5
29	39358.5
30	47453.0
31	55921.0
32	64044.5
33	71363.5
34	78371.5
35	84499.5
36	89230.5
37	93600.5
38	96379.5
39	97027.5
40	97879.5
41	98085.0
42	96470.0
43	94856.0
44	93146.5
45	90723.0
46	89336.0
47	87171.0
48	84332.5
49	82125.5
50	79397.0
51	75592.0
52	70226.0
53	66086.5
54	63160.5
55	59659.5
56	54948.5
57	49571.0
58	45034.5
59	40964.5
60	36662.5
61	31778.0
62	26801.0
63	22119.0
64	17893.5
65	13907.0
66	10401.5
67	7614.0
68	5642.0
69	4282.5
70	2970.5
71	2028.0
72	1541.5
73	1115.5
74	820.5
75	594.0
76	401.0
77	291.5
78	186.5
79	115.0
80	83.5
81	48.5
82	31.5
83	28.5
84	24.0
85	20.0
86	18.5
87	11.5
88	7.5
89	9.0
90	5.5
91	5.0
92	4.5
93	2.5
94	3.0
95	2.5
96	2.0
97	1.5
98	2.5
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32934728186234224
2	0.11903616684606044
3	0.06548314746320029
4	0.03912324541902018
5	0.042872714244269944
6	0.009809216421612999
7	0.0067793426234313775
8	0.014922128456044485
9	0.017346027494589782
10-11	0.020091850624191877
12-13	0.015338736103294458
14-15	0.017800508564317023
16-17	0.018539040302623794
18-19	0.01855797701386243
20-21	0.022591496507691714
22-23	0.014884255033567212
24-25	0.023519395358384834
26-27	0.018046685810419284
28-29	0.017800508564317023
30-31	0.018842027682441958
32-33	0.023367901668475754
34-35	0.014410837252601337
36-37	0.009032811260828959
38-39	0.01257397626245373
40-41	0.00859726690234035
42-43	0.007801925030317674
44-45	0.008767697303488066
46-47	0.007631494629169958
48-49	0.0039388359376361076
50-51	0.005851443772738256
52-53	0.007347443960590431
54-55	0.0029730636644657157
56-57	0.002689012995886189
58-59	0.002745823129602094
60-61	0.00354116500162477
62-63	0.0026322028621702832
64-65	0.0021019749474885
66-67	0.0015717470328067162
68-69	0.0032192409105679725
70-71	0.001874734412624878
72-73	0.0014960001878521754
74-75	0.002007291391295324
76-77	0.0035790384241020396
78-79	0.003086683931897527
80-81	0.0035032915791474994
82-83	0.0022345319261589456
84-85	0.0027647598408407294
86-87	0.00213984836996577
88-89	0.5984947586970634
90-91	0.60853121565354
92-93	0.6051983544755402
94-95	0.5927948086142342
96-97	0.5908632640678934
98-99	0.5877955168472345
100	0.5897270613935753
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2640374.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.95231717162588
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.11448007426426	53.760122428746804
2	13.251896125876335	18.00994097341978
3	3.7617056984457538	7.66849856181295
4	1.5052365802924195	4.09137254089456
5	0.7321269331149665	2.4874860784458965
6	0.4101076192986485	1.6720657812649296
7	0.26048552118324636	1.239041632784216
8	0.18718719897322575	1.0175843132077504
9	0.13663875186134797	0.8356427823975663
>10	0.6020836452161267	7.042351178709436
>50	0.03087581993312858	1.419753427098747
>100	0.007176031540589092	0.7561403012173445
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.7873422477270264E-5	0.0
3	0.0	0.0	0.0	3.7873422477270264E-5	0.0
4	0.0	0.0	0.0	3.7873422477270264E-5	0.0
5	0.0	0.0	0.0	3.7873422477270264E-5	0.0
6	0.0	0.0	0.0	3.7873422477270264E-5	0.0
7	0.0	0.0	0.0	3.7873422477270264E-5	0.0
8	0.0	0.0	0.0	3.7873422477270264E-5	0.0
9	0.0	0.0	0.0	1.136202674318108E-4	0.0
10-11	0.0	0.0	0.0	1.8936711238635133E-4	0.0
12-13	0.0	0.0	0.0	1.8936711238635133E-4	0.0
14-15	0.0	0.0	0.0	1.8936711238635133E-4	0.0
16-17	0.0	0.0	0.0	2.461772461022567E-4	0.0
18-19	0.0	0.0	0.0	2.6511395734089187E-4	0.0
20-21	0.0	0.0	0.0	2.6511395734089187E-4	0.0
22-23	0.0	0.0	0.0	3.4086080229543236E-4	0.0
24-25	0.0	0.0	0.0	4.166076472499729E-4	0.0
26-27	0.0	0.0	0.0	4.5448106972724315E-4	0.0
28-29	0.0	0.0	0.0	0.0010225824068862972	0.0
30-31	0.0	0.0	0.0	0.003673721980295216	0.0
32-33	0.0	0.0	0.0	0.00944941890807893	0.0
34-35	0.0	0.0	0.0	0.017667951585646578	0.0
36-37	0.0	0.0	0.0	0.03376415613848644	1.8936711238635132E-5
38-39	0.0	0.0	0.0	0.0624154002425414	3.7873422477270264E-5
40-41	0.0	0.0	0.0	0.09555464491015289	3.7873422477270264E-5
42-43	0.0	0.0	0.0	0.12950816816102567	3.7873422477270264E-5
44-45	0.0	0.0	0.0	0.17012741376789803	3.7873422477270264E-5
46-47	0.0	0.0	0.0	0.21977947063559936	3.7873422477270264E-5
48-49	0.0	0.0	0.0	0.2767789714638911	3.7873422477270264E-5
50-51	0.0	0.0	0.0	0.34099335927410285	3.7873422477270264E-5
52-53	0.0	0.0	0.0	0.4028217214682466	3.7873422477270264E-5
54-55	0.0	0.0	0.0	0.4723194517140375	3.7873422477270264E-5
56-57	0.0	0.0	0.0	0.5535958163502595	3.7873422477270264E-5
58-59	0.0	0.0	0.0	0.6481089421422874	3.7873422477270264E-5
60-61	0.0	0.0	0.0	0.7501778157185308	3.7873422477270264E-5
62-63	0.0	0.0	0.0	0.8530609678780354	3.7873422477270264E-5
64-65	0.0	0.0	0.0	0.9622689815912443	3.7873422477270264E-5
66-67	0.0	0.0	0.0	1.0853007945086568	3.7873422477270264E-5
68-69	0.0	0.0	0.0	1.2283487112053064	3.7873422477270264E-5
70-71	0.0	5.681013371590539E-5	0.0	1.3852961739511145	3.7873422477270264E-5
72-73	0.0	7.574684495454053E-5	0.0	1.552431587343308	3.7873422477270264E-5
74-75	0.0	7.574684495454053E-5	0.0	1.7168969244508543	3.7873422477270264E-5
76-77	0.0	7.574684495454053E-5	0.0	1.8900352753056953	3.7873422477270264E-5
78-79	0.0	7.574684495454053E-5	0.0	2.0959720100258523	3.7873422477270264E-5
80-81	0.0	7.574684495454053E-5	0.0	2.3263181655326104	3.7873422477270264E-5
82-83	0.0	7.574684495454053E-5	0.0	2.5688784997882874	3.7873422477270264E-5
84-85	0.0	7.574684495454053E-5	0.0	2.801307693531295	3.7873422477270264E-5
86-87	0.0	7.574684495454053E-5	0.0	3.0472008889649724	3.7873422477270264E-5
88	0.0	7.574684495454053E-5	0.0	3.250562230956675	3.7873422477270264E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAACGC	2850	0.0	36.756756	4
ATCAACG	3005	0.0	34.867428	3
GGTATCA	1905	0.0	34.36575	1
CAACGCA	3085	0.0	34.109085	5
GTATCAA	3205	0.0	34.09306	1
AACGCAG	3170	0.0	33.776363	6
GTACATG	6675	0.0	28.788227	1
TATCAAC	3895	0.0	27.52012	2
TACATGG	6735	0.0	27.014462	2
ACGCAGA	3850	0.0	26.957344	7
CGCAGAG	4030	0.0	25.403702	8
ACATGGG	6860	0.0	25.136307	3
GAGTACA	4975	0.0	19.975395	1
CGCTACG	145	6.510701E-4	19.488987	1
CATGGGG	4395	0.0	19.346254	4
TATACCG	170	8.666561E-5	19.343111	5
GCAGAGT	5095	0.0	19.171879	9
GTCTCGC	540	0.0	18.316038	1
GTATAGG	1180	0.0	17.562109	1
CTGTCGC	700	0.0	16.772102	9
>>END_MODULE