FastQCFastQC Report
Wed 25 May 2016
SRR1033154_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033154_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2640374
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40070.15175880386642193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35420.13414766241449128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34950.049.011481
GTATCAA60500.040.758621
TCAACGC69600.034.3171464
ATCAACG70650.033.7405783
CAACGCA72750.032.831255
AACGCAG74750.032.127526
TATCAAC77950.031.1839522
ACGCAGA87350.027.4932147
CGCAGAG88700.026.8093418
GTACATG66900.026.5191041
GTCGTAC1807.406925E-926.1439441
ATAACGC2550.025.8133623
TACATGG66450.025.4013122
GGACCGT2101.4333636E-924.6173256
GCAGAGT100950.023.4164439
TATAACG2504.3655746E-1022.5682532
GTGGTAT19450.022.5012651
ACATGGG71050.022.4994323
TAACGCA2901.2187229E-1021.0766724
TGGTATC19300.020.4634422
Produced by FastQC (version 0.11.5)