##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1033154_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2640374 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.71798995142355 34.0 31.0 34.0 31.0 34.0 2 32.80269840560466 34.0 31.0 34.0 31.0 34.0 3 32.905656168406445 34.0 31.0 34.0 31.0 34.0 4 36.347952221920075 37.0 37.0 37.0 35.0 37.0 5 36.287628570800955 37.0 37.0 37.0 35.0 37.0 6 36.37036154726565 37.0 37.0 37.0 35.0 37.0 7 36.33515214132543 37.0 37.0 37.0 35.0 37.0 8 36.327324841102055 37.0 37.0 37.0 35.0 37.0 9 38.1422169738075 39.0 39.0 39.0 37.0 39.0 10-11 38.11990024897988 39.0 39.0 39.0 37.0 39.0 12-13 38.080261925015165 39.0 39.0 39.0 36.0 39.0 14-15 39.59100510003507 41.0 40.0 41.0 37.0 41.0 16-17 39.604072377625286 41.0 40.0 41.0 37.0 41.0 18-19 39.62271689540951 41.0 40.0 41.0 37.0 41.0 20-21 39.6273796818178 41.0 40.0 41.0 37.0 41.0 22-23 39.55734585327684 41.0 40.0 41.0 37.0 41.0 24-25 39.513024291255704 41.0 39.0 41.0 37.0 41.0 26-27 39.413345798739115 41.0 39.0 41.0 36.0 41.0 28-29 39.32764240974953 41.0 39.0 41.0 36.0 41.0 30-31 39.243529704503985 41.0 39.0 41.0 36.0 41.0 32-33 39.291572709017736 41.0 39.0 41.0 36.0 41.0 34-35 39.2410088495039 41.0 39.0 41.0 35.0 41.0 36-37 39.161528821295775 41.0 39.0 41.0 35.0 41.0 38-39 39.08285133090994 41.0 39.0 41.0 35.0 41.0 40-41 38.978591290476274 41.0 39.0 41.0 35.0 41.0 42-43 38.87160644666248 41.0 39.0 41.0 35.0 41.0 44-45 38.74011162812541 41.0 39.0 41.0 35.0 41.0 46-47 38.55134537758666 41.0 39.0 41.0 35.0 41.0 48-49 38.384935050867796 41.0 38.0 41.0 35.0 41.0 50-51 38.26514766468689 40.0 38.0 41.0 34.0 41.0 52-53 38.14234422850703 40.0 38.0 41.0 34.0 41.0 54-55 38.01416901545008 40.0 38.0 41.0 34.0 41.0 56-57 37.86451408020227 40.0 37.5 41.0 34.0 41.0 58-59 37.68565949369294 40.0 37.0 41.0 33.5 41.0 60-61 37.50339099688151 40.0 36.5 41.0 33.0 41.0 62-63 37.27576131260193 39.5 36.0 41.0 33.0 41.0 64-65 37.03137112393926 39.0 35.5 41.0 33.0 41.0 66-67 36.76043753650051 39.0 35.0 41.0 32.5 41.0 68-69 36.44359018835968 38.5 35.0 41.0 32.0 41.0 70-71 36.11386758088059 37.5 35.0 40.0 32.0 41.0 72-73 35.762959148969045 37.0 35.0 39.5 31.0 41.0 74-75 35.42585387524646 36.5 35.0 39.0 31.0 41.0 76-77 35.081355898823425 36.0 35.0 39.0 31.0 40.5 78-79 34.72916090675034 35.5 35.0 38.0 31.0 39.5 80-81 34.37845774878862 35.0 35.0 37.0 31.0 39.0 82-83 34.04637941443144 35.0 34.0 37.0 30.5 39.0 84-85 33.7621908865941 35.0 34.0 36.0 30.0 38.0 86-87 33.515651949307184 35.0 34.0 36.0 30.0 37.0 88-89 33.288825560318344 35.0 34.0 36.0 30.0 37.0 90-91 33.07968681709485 35.0 34.0 35.0 30.0 36.5 92-93 32.90911514808129 35.0 34.0 35.0 30.0 36.0 94-95 32.754702742869 35.0 34.0 35.0 29.0 36.0 96-97 32.62502793164908 35.0 34.0 35.0 29.0 36.0 98-99 32.50974502097051 35.0 34.0 35.0 29.0 36.0 100 32.40834556013655 35.0 34.0 35.0 29.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02947741755137656 1101 2 0.09289224351994818 1101 3 0.0866278526898725 1101 4 0.06294564694781002 1101 5 0.040934880885579616 1101 6 0.09974175869345459 1101 7 0.10794066512109879 1101 8 0.11775867033314569 1101 9 0.11296161278087169 1101 10-11 0.12857185785528458 1101 12-13 0.17784905181305533 1101 14-15 0.14041173313960797 1101 16-17 0.12417217137066672 1101 18-19 0.189935606613993 1101 20-21 0.14495409023454187 1101 22-23 0.19317157069873048 1101 24-25 0.14850485493949606 1101 26-27 0.15968272510382064 1101 28-29 0.22384768663952315 1101 30-31 0.20185258477646784 1101 32-33 0.15969930332451554 1101 34-35 0.15978721231876136 1101 36-37 0.18909116123434444 1101 38-39 0.18051208938755536 1101 40-41 0.28383718672244385 1101 42-43 0.2379194702077001 1101 44-45 0.13116079821775628 1101 46-47 0.11687386071905337 1101 48-49 0.010800273719127063 1101 50-51 0.013141286947188746 1101 52-53 0.007495281736936477 1101 54-55 0.04062652368341446 1101 56-57 0.07323593439407716 1101 58-59 0.08113502864188149 1101 60-61 0.10472994106242339 1101 62-63 0.02966157892974053 1101 64-65 0.057821596306617096 1101 66-67 0.034758641797509426 1101 68-69 0.039817759282492204 1101 70-71 0.10409060660651193 1101 72-73 0.08365862155960002 1101 74-75 0.039374447107249466 1101 76-77 0.0407707082296227 1101 78-79 4.057655939391225E-4 1101 80-81 -0.0141670106041758 1101 82-83 0.016576810025206612 1101 84-85 0.04786078413286532 1101 86-87 -0.014953624673594845 1101 88-89 0.013931100269793717 1101 90-91 0.08503031580644915 1101 92-93 -0.02027439401415876 1101 94-95 -0.02947303155437453 1101 96-97 -0.021869635814404376 1101 98-99 -0.029313323282913473 1101 100 -0.02297608267348039 1102 1 0.11034528326108983 1102 2 0.12211181324600773 1102 3 0.11763692639467394 1102 4 0.08450886837457716 1102 5 0.05790697683494983 1102 6 0.060680746256323914 1102 7 0.10633418202105815 1102 8 0.14830122170751991 1102 9 0.1333533649012537 1102 10-11 0.1472143208399146 1102 12-13 0.15811554920801996 1102 14-15 0.20205317262444566 1102 16-17 0.15244060540995719 1102 18-19 0.1633482057773179 1102 20-21 0.18957353269023258 1102 22-23 0.22779508062007636 1102 24-25 0.15848348793031874 1102 26-27 0.15075744209610775 1102 28-29 0.15480411545895123 1102 30-31 0.17176238442890224 1102 32-33 0.20937066476317057 1102 34-35 0.23375749750720587 1102 36-37 0.21894312538456262 1102 38-39 0.17528257683366633 1102 40-41 0.1603504068149988 1102 42-43 0.15730785634195854 1102 44-45 0.15978114040046165 1102 46-47 0.24297042187131979 1102 48-49 0.15634385854334454 1102 50-51 0.1926333602888164 1102 52-53 0.20061890540560512 1102 54-55 0.21110647709299712 1102 56-57 0.20534361579845495 1102 58-59 0.20577273700338594 1102 60-61 0.1744680568790642 1102 62-63 0.1731583149317686 1102 64-65 0.1522315748340688 1102 66-67 0.21765401400755735 1102 68-69 0.2575507939329924 1102 70-71 0.2604983424498002 1102 72-73 0.23701858488306016 1102 74-75 0.20406960653667738 1102 76-77 0.21817583097497106 1102 78-79 0.19719791010867027 1102 80-81 0.15603705466040907 1102 82-83 0.11123444715933317 1102 84-85 0.234171365136703 1102 86-87 0.222524031024939 1102 88-89 0.22648237513684677 1102 90-91 0.14445828058261867 1102 92-93 0.13688679536827664 1102 94-95 0.13141362771607135 1102 96-97 0.035285304626164304 1102 98-99 0.07230954727142347 1102 100 0.07093171912666918 1103 1 0.07591238407742651 1103 2 0.1285278183044909 1103 3 0.08617585688436691 1103 4 0.07888179968470865 1103 5 0.07977989290344567 1103 6 0.081957496424927 1103 7 0.12499241826997576 1103 8 0.11200332630233589 1103 9 0.1253067945753017 1103 10-11 0.12314145534463705 1103 12-13 0.12594400149050955 1103 14-15 0.19349194288820826 1103 16-17 0.18252020161389026 1103 18-19 0.14809654886578727 1103 20-21 0.1557556700095759 1103 22-23 0.12863978687881428 1103 24-25 0.11557906695444586 1103 26-27 0.12973210642436328 1103 28-29 0.17195517115307268 1103 30-31 0.18401840567546657 1103 32-33 0.17218062530618283 1103 34-35 0.22597852684925357 1103 36-37 0.18236347735905412 1103 38-39 0.1378456953721141 1103 40-41 0.1332432209183665 1103 42-43 0.12627754463733254 1103 44-45 0.17528813108796015 1103 46-47 0.21083792178640692 1103 48-49 0.23173714368599008 1103 50-51 0.21098676371821057 1103 52-53 0.2829869106173746 1103 54-55 0.23047896566974657 1103 56-57 0.15808850469559843 1103 58-59 0.18141928015566577 1103 60-61 0.14425164880256602 1103 62-63 0.07352508687704784 1103 64-65 0.17998377246096453 1103 66-67 0.1756600270526505 1103 68-69 0.21564883745789842 1103 70-71 0.2874206690151695 1103 72-73 0.2791840040093163 1103 74-75 0.23615594622320657 1103 76-77 0.15070848945683224 1103 78-79 0.19888936936079915 1103 80-81 0.25308363108301535 1103 82-83 0.16004943134721827 1103 84-85 0.16377349153432874 1103 86-87 0.05948564908916154 1103 88-89 0.10057270319792622 1103 90-91 0.12379017994366137 1103 92-93 0.0610646394802572 1103 94-95 0.06893596397802781 1103 96-97 0.10161162786913991 1103 98-99 0.14874884446049208 1103 100 0.11071951483990716 1104 1 0.04102199653235061 1104 2 0.052282309888838085 1104 3 0.07743936283512198 1104 4 0.015052271928745142 1104 5 -0.012581148583983293 1104 6 0.07838982525335325 1104 7 0.02477082942434805 1104 8 0.030020842116385893 1104 9 0.0699917607803826 1104 10-11 0.07052502370783031 1104 12-13 0.11617216879930226 1104 14-15 0.10524304356478353 1104 16-17 0.10499005646936865 1104 18-19 0.08979979366899471 1104 20-21 0.08879286378098783 1104 22-23 0.08089757244530205 1104 24-25 0.058716945671640985 1104 26-27 0.012980480349938261 1104 28-29 0.030569547427838017 1104 30-31 -0.03529520493652427 1104 32-33 -0.05653260801200588 1104 34-35 0.03849803428274612 1104 36-37 0.01607034421672182 1104 38-39 0.010105135835466683 1104 40-41 -0.045189757673341546 1104 42-43 -0.020005056440773217 1104 44-45 -0.007693154964002247 1104 46-47 0.012492116694687638 1104 48-49 -0.0818157525323997 1104 50-51 -0.011417122155449988 1104 52-53 -0.0084761968818583 1104 54-55 -0.07405187107134026 1104 56-57 -0.019444526237364812 1104 58-59 -0.027635175310237514 1104 60-61 -0.09676468617805511 1104 62-63 -0.04430651183981382 1104 64-65 0.007128271332810243 1104 66-67 0.05090416688137367 1104 68-69 -0.008111404432156633 1104 70-71 0.013616636880954047 1104 72-73 0.0019324473498016914 1104 74-75 -0.04234263265006888 1104 76-77 -0.10015026304022712 1104 78-79 -0.06665120404466052 1104 80-81 -0.0872993690563888 1104 82-83 -0.10520486759125447 1104 84-85 -0.08510346170837835 1104 86-87 -0.09848418243894486 1104 88-89 -0.0807339392630908 1104 90-91 -0.050366088548962296 1104 92-93 -0.060825710769378816 1104 94-95 -0.07885369934051312 1104 96-97 -0.10837216491904655 1104 98-99 -0.11358804290682656 1104 100 -0.1531769036484789 1105 1 0.09779873219419954 1105 2 0.11668682487068338 1105 3 0.11481223488494408 1105 4 0.10576101003783123 1105 5 0.08815752525434561 1105 6 0.0505370471099198 1105 7 0.0371241433279863 1105 8 0.023125734483492977 1105 9 0.09831079694402689 1105 10-11 0.03295220499530416 1105 12-13 0.012330314571087797 1105 14-15 0.04196099906837958 1105 16-17 0.09382891985290343 1105 18-19 0.029505388151370937 1105 20-21 0.04631877704580489 1105 22-23 0.11840290105967455 1105 24-25 0.042082222271908165 1105 26-27 0.06599833470365013 1105 28-29 0.09596642944669043 1105 30-31 0.1264894733447548 1105 32-33 0.10653284749329828 1105 34-35 0.07626541136312426 1105 36-37 0.02696687463227221 1105 38-39 0.06512631047009876 1105 40-41 0.1264922292569821 1105 42-43 0.04392307189067424 1105 44-45 0.09588075095636128 1105 46-47 0.09375070642104788 1105 48-49 0.09273335290833984 1105 50-51 0.11752245459739896 1105 52-53 0.09277821862114877 1105 54-55 0.06122775073856701 1105 56-57 0.0701321584062029 1105 58-59 0.037421527967282486 1105 60-61 0.022126505051168976 1105 62-63 0.021555366383353203 1105 64-65 -0.041442227684676425 1105 66-67 0.001392523731091444 1105 68-69 0.012924396353717782 1105 70-71 0.12543671421321534 1105 72-73 0.08020994589871577 1105 74-75 0.03922793252749557 1105 76-77 0.044652862989259745 1105 78-79 0.06436382111321848 1105 80-81 0.08190185374551362 1105 82-83 0.04655775278428109 1105 84-85 0.04491172782660158 1105 86-87 -0.014959439593610568 1105 88-89 0.008684472745024152 1105 90-91 0.05828550089571394 1105 92-93 0.13902426955311853 1105 94-95 0.1185660917982716 1105 96-97 0.11041883130846486 1105 98-99 0.010053578192369628 1105 100 0.011704100293158604 1106 1 0.06891590501814449 1106 2 0.05380161983452325 1106 3 0.05208717144767405 1106 4 0.050134663552199754 1106 5 -0.014936757792739286 1106 6 0.0183908559641921 1106 7 0.01844619268072023 1106 8 0.029505091286381457 1106 9 0.0061554366062708255 1106 10-11 0.03381082703686644 1106 12-13 0.06612697305189386 1106 14-15 0.08909396640026301 1106 16-17 0.05177783034090311 1106 18-19 0.03279403763666977 1106 20-21 0.06213531402843131 1106 22-23 0.05754564545758711 1106 24-25 0.05233747827498547 1106 26-27 0.0846089850139009 1106 28-29 0.013728035562202479 1106 30-31 0.00314398253119208 1106 32-33 0.032351274504357264 1106 34-35 0.06019621078129944 1106 36-37 0.05788550507680412 1106 38-39 0.07304482725751171 1106 40-41 0.09897571385644 1106 42-43 0.06913222013914577 1106 44-45 0.041008755686547715 1106 46-47 0.1119615774048981 1106 48-49 0.06475524914323216 1106 50-51 -0.01982987096766209 1106 52-53 0.05472575245329381 1106 54-55 0.05908295157324517 1106 56-57 -0.04069483119991446 1106 58-59 -0.06526940002392223 1106 60-61 -0.11635000432170983 1106 62-63 -0.040621588806367015 1106 64-65 -0.022954876189189122 1106 66-67 0.016972296340235005 1106 68-69 -0.019209471295759784 1106 70-71 -0.08574395334994023 1106 72-73 -0.0543651233491218 1106 74-75 -0.04626539682145392 1106 76-77 -0.05290050796585177 1106 78-79 -0.07951459386806192 1106 80-81 -0.04785013110360836 1106 82-83 -0.01665514231787313 1106 84-85 -0.06831879051370038 1106 86-87 -0.04933733290150144 1106 88-89 -0.07492917223867579 1106 90-91 -0.04461677824871657 1106 92-93 -0.0629389974887431 1106 94-95 -0.08031119299708678 1106 96-97 0.029921945385730453 1106 98-99 0.03289214549972996 1106 100 0.06431125405080707 1107 1 -0.02277877722171695 1107 2 0.019452769597577912 1107 3 3.080748192729743E-5 1107 4 0.023069247834030193 1107 5 -0.09892362396420395 1107 6 -0.08511203088730923 1107 7 -0.04658152301092855 1107 8 -0.03105561838084725 1107 9 0.022600866890819304 1107 10-11 0.013002550404493718 1107 12-13 0.037181459808309114 1107 14-15 0.05900103753339181 1107 16-17 0.023011385709487797 1107 18-19 -0.007465797433404475 1107 20-21 -0.01621039925088752 1107 22-23 0.06643074240167834 1107 24-25 0.0321128645383979 1107 26-27 0.07553209907237601 1107 28-29 0.03409048419827343 1107 30-31 0.05812363851789115 1107 32-33 0.10051594781904782 1107 34-35 0.12461513006228131 1107 36-37 0.1359925421414232 1107 38-39 0.11169206159994616 1107 40-41 0.1541380026212309 1107 42-43 0.13585992007472214 1107 44-45 0.22290903622327818 1107 46-47 0.2048855896331787 1107 48-49 0.18188320491216814 1107 50-51 0.12016196340530172 1107 52-53 0.16989808724990496 1107 54-55 0.1258642983511038 1107 56-57 0.18159998529839072 1107 58-59 0.20669213008972065 1107 60-61 0.19316701924086033 1107 62-63 0.19196948853774387 1107 64-65 0.12504016986597577 1107 66-67 0.10611294643918256 1107 68-69 0.18734230140974972 1107 70-71 0.15806392286682325 1107 72-73 0.18610269923801326 1107 74-75 0.14924588032243946 1107 76-77 0.13485649605033956 1107 78-79 0.20956224537060564 1107 80-81 0.23641551429432894 1107 82-83 0.21143089074825383 1107 84-85 0.1589429984567019 1107 86-87 0.16536476550732715 1107 88-89 0.22229535030442804 1107 90-91 0.2636962331937269 1107 92-93 0.23810919912932604 1107 94-95 0.1458251798569421 1107 96-97 0.1434393396215583 1107 98-99 0.14695454986640044 1107 100 0.15933240921910397 1108 1 0.0352628089871132 1108 2 0.023339694551033574 1108 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0.0028687517156669173 2213 100 0.009445086214277865 2214 1 -0.12401270639366402 2214 2 -0.08849602611199003 2214 3 -0.13161896115978777 2214 4 -0.08013305677733484 2214 5 -0.08460770088952785 2214 6 -0.05511047327119911 2214 7 -0.05613543386574804 2214 8 -0.048273220653754834 2214 9 -0.07439838292163614 2214 10-11 -0.05671626411204045 2214 12-13 -0.07073970610312585 2214 14-15 -0.11989664910397124 2214 16-17 -0.1564117074078908 2214 18-19 -0.13217382697197166 2214 20-21 -0.13809149314872826 2214 22-23 -0.149386216240039 2214 24-25 -0.11519950463974027 2214 26-27 -0.1482126852528296 2214 28-29 -0.1158457526122092 2214 30-31 -0.06342548500656875 2214 32-33 -0.10504256763519493 2214 34-35 -0.13425155836331726 2214 36-37 -0.19219995169002857 2214 38-39 -0.17494620655109117 2214 40-41 -0.08577761641559078 2214 42-43 -0.1047475447041677 2214 44-45 -0.15354077138636768 2214 46-47 -0.09260788681737608 2214 48-49 -0.14333385687344702 2214 50-51 -0.051250332075021277 2214 52-53 -0.09373135642796626 2214 54-55 -0.10643755003836475 2214 56-57 -0.10326659084675072 2214 58-59 -0.15890915651219473 2214 60-61 -0.1872162093615941 2214 62-63 -0.24392470144407952 2214 64-65 -0.1815559215633371 2214 66-67 -0.2630228077855108 2214 68-69 -0.24304453227592404 2214 70-71 -0.13869023632594235 2214 72-73 -0.2864998516198085 2214 74-75 -0.2563900205823586 2214 76-77 -0.1875351436791064 2214 78-79 -0.299267291433992 2214 80-81 -0.23353818102605572 2214 82-83 -0.2584552436613592 2214 84-85 -0.27852848423421506 2214 86-87 -0.2140642718694039 2214 88-89 -0.32900407579933955 2214 90-91 -0.18631089441355897 2214 92-93 -0.2722832100514694 2214 94-95 -0.2870232376766353 2214 96-97 -0.4448389386787497 2214 98-99 -0.5242008490346777 2214 100 -0.6725712013130263 2215 1 -0.03079051900373031 2215 2 -0.06961856463562555 2215 3 -0.05282750989184848 2215 4 -0.010266526319725244 2215 5 -0.041082371694109554 2215 6 -0.07151777385060853 2215 7 -0.04417776357316683 2215 8 -0.021806865218323424 2215 9 -0.12679053427829956 2215 10-11 -0.11901036189448888 2215 12-13 -0.10521726005316623 2215 14-15 -0.13789415812438932 2215 16-17 -0.11767794325693615 2215 18-19 -0.10226464242281708 2215 20-21 -0.09909495364951226 2215 22-23 -0.14380342021834025 2215 24-25 -0.1415424004458643 2215 26-27 -0.11119367852185746 2215 28-29 -0.14024972704396532 2215 30-31 -0.13100861751626525 2215 32-33 -0.142909935681665 2215 34-35 -0.1267536856211393 2215 36-37 -0.0727630486385209 2215 38-39 -0.14222044790982835 2215 40-41 -0.1510904231734287 2215 42-43 -0.1145742049638443 2215 44-45 -0.11704223172012007 2215 46-47 -0.18807582907215448 2215 48-49 -0.0567171227679637 2215 50-51 -0.015301308798044033 2215 52-53 -0.0909812326657331 2215 54-55 -0.035873714752874264 2215 56-57 -0.12111643246741721 2215 58-59 -0.0982090193751759 2215 60-61 -0.09540218797886979 2215 62-63 -0.06663146908942252 2215 64-65 -0.03270718260662164 2215 66-67 -0.08757880701569576 2215 68-69 -0.02163045957272658 2215 70-71 -0.08080316315432157 2215 72-73 4.7660995459608557E-4 2215 74-75 -0.06977607037048017 2215 76-77 -0.15428165564197371 2215 78-79 -0.1679225860970206 2215 80-81 -0.1563445636468117 2215 82-83 -0.07824231211246513 2215 84-85 -0.10035178929690858 2215 86-87 -0.09774012498149176 2215 88-89 -0.14504499177296282 2215 90-91 -0.12892746390620147 2215 92-93 -0.057149480275548115 2215 94-95 -0.007329804881649693 2215 96-97 0.01529909402836438 2215 98-99 0.019697037747995694 2215 100 0.029966378224628443 2216 1 -0.026266169710105203 2216 2 -0.05328391642913033 2216 3 -0.07774823706590439 2216 4 -0.07470279775134969 2216 5 -0.0707350838377252 2216 6 -0.118284570110049 2216 7 -0.1158464485380506 2216 8 -0.0867777183030185 2216 9 -0.17519600229277899 2216 10-11 -0.1212319948734546 2216 12-13 -0.0828224002507909 2216 14-15 -0.19249718437998808 2216 16-17 -0.1542405690186115 2216 18-19 -0.15480130409950732 2216 20-21 -0.15957876769228108 2216 22-23 -0.17837485193279434 2216 24-25 -0.2291096528620855 2216 26-27 -0.2661741616850364 2216 28-29 -0.26154283436456893 2216 30-31 -0.14034472879799154 2216 32-33 -0.18799673931148675 2216 34-35 -0.11734856870804578 2216 36-37 -0.17770525900050416 2216 38-39 -0.21966853211549164 2216 40-41 -0.24984969687942282 2216 42-43 -0.1381781185984039 2216 44-45 -0.01548931854117086 2216 46-47 -0.041032116491265924 2216 48-49 -0.06699332405264613 2216 50-51 -0.028655560012460057 2216 52-53 -0.017038822460143876 2216 54-55 0.0019352980105367124 2216 56-57 0.014595528722594509 2216 58-59 -0.02413549617818944 2216 60-61 0.03460774461516536 2216 62-63 0.07521828060186664 2216 64-65 0.06261397890643394 2216 66-67 0.0486079660875518 2216 68-69 0.1372379214098629 2216 70-71 0.13385346335721948 2216 72-73 0.11586468240798098 2216 74-75 0.11891273554562787 2216 76-77 0.13202960719545587 2216 78-79 0.1416032161879741 2216 80-81 0.0947132553260488 2216 82-83 0.12161287002481913 2216 84-85 0.12307144004746817 2216 86-87 0.08486768285329305 2216 88-89 0.11203714945046528 2216 90-91 0.11923195812408238 2216 92-93 0.12302321485385903 2216 94-95 0.08988157465345381 2216 96-97 0.11687350414594988 2216 98-99 0.143505195217422 2216 100 0.1309513872038437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11.0 3 14.0 4 5.0 5 16.0 6 45.0 7 119.0 8 280.0 9 493.0 10 995.0 11 1586.0 12 2361.0 13 3683.0 14 4476.0 15 5808.0 16 7295.0 17 10543.0 18 12070.0 19 6648.0 20 4690.0 21 4405.0 22 4722.0 23 5129.0 24 5869.0 25 7206.0 26 9021.0 27 11568.0 28 15458.0 29 19758.0 30 25411.0 31 32157.0 32 41928.0 33 54926.0 34 76291.0 35 116072.0 36 214915.0 37 499333.0 38 947499.0 39 485568.0 40 2000.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.99883092720664 19.879923638773185 13.366967552543832 23.754277881476337 2 15.557105021063599 20.104479087328624 40.62178426466016 23.71663162694762 3 18.07047031973501 25.33538809274747 30.366682901740432 26.227458685777087 4 11.897238605664587 16.685016374605215 39.35211328416596 32.06563173556423 5 12.093270145915046 38.1076509920647 36.48531604823017 13.313762813790092 6 30.737594712248228 38.822685945812054 17.47920553438425 12.960513807555468 7 27.0304613708791 32.738644876909945 22.688998288806186 17.54189546340477 8 25.016541298708777 36.892947593151085 20.217538435085203 17.872972673054935 9 24.940197820610127 16.01062274558282 21.393955094206422 37.655224339600636 10-11 23.641766138619786 26.755698751571284 29.364851522084685 20.237683587724245 12-13 24.53701634692661 24.058788641306116 29.59201991839035 21.812175093376922 14-15 21.518184088867798 26.167851758739253 26.883584374395802 25.430379777997146 16-17 20.75692685960398 29.745823887070543 27.394376705724266 22.10287254760121 18-19 20.50462236265706 28.30335742654298 30.134975375614896 21.057044835185064 20-21 21.70327006704353 27.626616532354888 29.79986926094561 20.870244139655973 22-23 21.2668380821014 27.403404599887367 29.91276976816498 21.416987549846255 24-25 21.182878328175605 27.531678289393202 29.843965227640183 21.441478154791007 26-27 20.916425724927226 27.529642083160695 29.956285833534697 21.597646358377386 28-29 20.97358594047983 27.551837075738582 29.99306478010505 21.481512203676537 30-31 21.248308098866698 28.029949846793556 29.57694183838259 21.144800215957147 32-33 20.886628256627855 27.658466981440327 30.123070098226123 21.331834663705695 34-35 21.047470324180832 27.44185024513119 30.127818269916695 21.382861160771284 36-37 21.37777762624334 27.962574024572824 29.416243885585438 21.2434044635984 38-39 21.227439189089363 28.031491469292526 29.54766812428235 21.193401217335765 40-41 21.026660329748328 27.742063682240797 29.801214862258213 21.43006112575266 42-43 21.084803690653224 27.701889895234377 29.629819041541634 21.58348737257077 44-45 21.224330073663154 27.995464784746797 29.417795719464436 21.362409422125612 46-47 21.319748018766205 28.121862521702518 29.164037995294244 21.394351464237033 48-49 21.368467980838542 28.114371815213946 28.895102479422114 21.622057724525405 50-51 21.337420694072335 28.05931357110703 28.881343503632245 21.72192223118839 52-53 21.39544246916975 28.067017811181515 28.87439264138055 21.663147078268185 54-55 21.511478718631587 28.475425957828705 28.613954217688402 21.399141105851307 56-57 21.293135543502263 28.151210217281218 28.72998673262354 21.825667506592975 58-59 21.139767288075124 27.796659983199174 29.18043695788355 21.883135770842156 60-61 21.49682912737517 28.238121938984488 28.671168701709977 21.593880231930363 62-63 21.267480223198444 28.1916426703975 28.78984191814618 21.75103518825788 64-65 21.25668013740915 27.912066477546027 28.947767101341142 21.883486283703686 66-67 21.162731156838024 27.97441334726577 28.85323256933427 22.009622926561935 68-69 21.603912521230665 28.123177912267856 28.69085026247532 21.58205930402616 70-71 21.536190579891965 27.933456106879262 28.724654285159705 21.80569902806906 72-73 21.321137526270082 28.069021622422085 28.835413036323445 21.77442781498439 74-75 21.426248963446078 28.13412340251394 28.622200878327362 21.81742675571262 76-77 21.423121693993387 28.132403688960366 28.519793384587622 21.924681232458624 78-79 21.571626135469092 28.196730500438672 28.573571125448726 21.658072238643513 80-81 21.418267109484567 28.032509166353265 28.660112264866612 21.88911145929556 82-83 21.3129394396344 28.10163971616808 28.809098588812798 21.77632225538472 84-85 21.262183668681097 27.986567026534363 28.706144615798895 22.04510468898565 86-87 21.395580922370726 28.170710923090326 28.53887902074331 21.894829133795643 88-89 21.17217106364477 28.024192784810033 28.62922070888442 22.174415442660774 90-91 21.152988175160033 27.91873423992207 28.705535655176124 22.222741929741773 92-93 21.273387785215274 28.074185702480026 28.575705562924043 22.076720949380658 94-95 21.022646791704506 27.821115493486907 28.755717939958508 22.40051977485008 96-97 20.949153415387364 27.851281674490053 28.76607632100604 22.433488589116543 98-99 21.032967299329563 27.90017626290821 28.633595089180545 22.43326134858168 100 21.140452072320056 27.793714072324605 28.492478717030238 22.573355138325102 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 40.0 1 35.5 2 106.5 3 198.5 4 149.5 5 109.5 6 255.0 7 584.0 8 987.5 9 1190.0 10 1011.5 11 821.0 12 857.0 13 974.0 14 1112.5 15 1305.0 16 1546.0 17 1784.0 18 2116.0 19 2543.0 20 3204.5 21 4182.5 22 5496.5 23 7643.5 24 10387.5 25 13546.0 26 18174.0 27 23997.0 28 31197.0 29 39400.0 30 47130.5 31 55075.5 32 63157.5 33 70327.5 34 77308.0 35 83730.0 36 88399.0 37 92541.5 38 95656.5 39 96283.5 40 96814.0 41 97208.5 42 95588.5 43 94390.5 44 93206.0 45 91376.0 46 89837.0 47 87055.5 48 83693.0 49 81496.0 50 79130.5 51 75524.0 52 70269.0 53 65800.5 54 62514.5 55 59304.0 56 54916.5 57 49176.5 58 44800.5 59 41035.5 60 36537.5 61 31450.0 62 26636.5 63 21873.5 64 17376.5 65 13296.5 66 9930.5 67 7260.5 68 5301.0 69 4076.0 70 2873.5 71 1851.0 72 1314.5 73 951.5 74 687.5 75 488.0 76 308.5 77 208.0 78 134.0 79 68.5 80 34.0 81 18.5 82 6.5 83 3.5 84 2.5 85 1.0 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09036598603076686 2 0.0014013166316589997 3 0.0 4 1.8936711238635133E-4 5 0.03900962515158837 6 3.029873798181621E-4 7 0.00424182331745427 8 4.923544922045134E-4 9 7.574684495454053E-5 10-11 5.68101337159054E-4 12-13 0.0 14-15 9.468355619317566E-5 16-17 0.0 18-19 1.3255697867044594E-4 20-21 0.0 22-23 1.8936711238635133E-4 24-25 3.7873422477270264E-5 26-27 0.0015149368990908106 28-29 0.00859726690234035 30-31 0.03641529571189536 32-33 0.026492459022850547 34-35 0.029048915040066296 36-37 0.026890129958861888 38-39 0.02556456017215743 40-41 0.0308857760302139 42-43 0.023651952337055283 44-45 0.02225063570539628 46-47 0.015736407039305796 48-49 0.010718178561067486 50-51 0.007669368051647229 52-53 0.005699950082829175 54-55 0.004488000563556527 56-57 0.002386025616068027 58-59 0.0010604558293635675 60-61 4.734177809658783E-4 62-63 0.0012498229417499188 64-65 0.003181367488090702 66-67 4.923544922045134E-4 68-69 0.0011551393855567431 70-71 4.3554435848860805E-4 72-73 4.923544922045134E-4 74-75 5.491646259204189E-4 76-77 3.597975135340675E-4 78-79 1.8936711238635132E-5 80-81 4.166076472499729E-4 82-83 3.2192409105679726E-4 84-85 2.0830382362498645E-4 86-87 1.5149368990908106E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2640374.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.210554088047 #Duplication Level Percentage of deduplicated Percentage of total 1 78.01312355887387 51.652921369723124 2 13.8677504196128 18.363828784746197 3 3.991832286972985 7.929042826411116 4 1.60425561903068 4.248746137395367 5 0.783244928293063 2.592954034446817 6 0.4596787604834708 1.8261351260470349 7 0.2862180555037347 1.3265459231433965 8 0.19352282481484448 1.0250602767739925 9 0.1353706091103313 0.8066666732788312 >10 0.6220259994231974 7.2727351566614225 >50 0.03326947828877994 1.5015309089719857 >100 0.009421647221012101 1.0383797768184606 >500 5.7162474275819836E-5 0.019415814156558773 >1k 2.2864989695376008E-4 0.3960371914256832 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4007 0.15175880386642193 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3542 0.13414766241449128 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.7873422477270264E-5 0.0 5 0.0 0.0 0.0 3.7873422477270264E-5 0.0 6 0.0 0.0 0.0 3.7873422477270264E-5 0.0 7 0.0 0.0 0.0 3.7873422477270264E-5 0.0 8 0.0 0.0 0.0 3.7873422477270264E-5 0.0 9 0.0 0.0 0.0 1.8936711238635133E-4 0.0 10-11 0.0 0.0 0.0 2.0830382362498648E-4 0.0 12-13 0.0 0.0 0.0 2.272405348636216E-4 0.0 14-15 0.0 0.0 0.0 2.272405348636216E-4 0.0 16-17 0.0 0.0 0.0 2.84050668579527E-4 0.0 18-19 0.0 0.0 0.0 3.029873798181621E-4 0.0 20-21 0.0 0.0 0.0 3.029873798181621E-4 0.0 22-23 0.0 0.0 0.0 3.2192409105679726E-4 0.0 24-25 0.0 0.0 0.0 4.1660764724997296E-4 0.0 26-27 0.0 0.0 0.0 5.112912034431486E-4 0.0 28-29 0.0 0.0 0.0 0.0010983292518408376 0.0 30-31 0.0 0.0 0.0 0.0037684055364883913 0.0 32-33 0.0 0.0 0.0 0.009581975886749378 0.0 34-35 0.0 0.0 0.0 0.01781944527555566 0.0 36-37 0.0 0.0 0.0 0.03397245996211143 0.0 38-39 0.0 0.0 0.0 0.06264264077740501 0.0 40-41 0.0 0.0 0.0 0.09563039175510743 0.0 42-43 0.0 0.0 0.0 0.1298490289633211 0.0 44-45 0.0 0.0 0.0 0.17067657839381845 0.0 46-47 0.0 0.0 0.0 0.220574812507622 0.0 48-49 0.0 0.0 0.0 0.2777447437370615 0.0 50-51 0.0 0.0 0.0 0.3421106252371823 0.0 52-53 0.0 0.0 0.0 0.40427984823362145 0.0 54-55 0.0 0.0 0.0 0.4743078063940942 0.0 56-57 0.0 0.0 0.0 0.5563416394798615 0.0 58-59 0.0 0.0 0.0 0.6515743602989577 0.0 60-61 0.0 0.0 0.0 0.7544385757472236 0.0 62-63 0.0 0.0 0.0 0.8577762089764556 0.0 64-65 0.0 0.0 0.0 0.9672114632245281 0.0 66-67 0.0 0.0 0.0 1.0912469218375882 0.0 68-69 0.0 0.0 0.0 1.234503142357863 0.0 70-71 0.0 0.0 0.0 1.392586807777989 0.0 72-73 0.0 0.0 0.0 1.5607637402883077 0.0 74-75 0.0 0.0 0.0 1.7262137863802627 0.0 76-77 0.0 0.0 0.0 1.9006208968880922 0.0 78-79 0.0 0.0 0.0 2.1091898344704196 0.0 80-81 0.0 0.0 0.0 2.3424522435079274 0.0 82-83 0.0 0.0 0.0 2.5883833123640816 0.0 84-85 0.0 0.0 0.0 2.82399387359518 0.0 86-87 0.0 0.0 0.0 3.0736554745653457 0.0 88 0.0 0.0 0.0 3.279989880221514 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3495 0.0 49.01148 1 GTATCAA 6050 0.0 40.75862 1 TCAACGC 6960 0.0 34.317146 4 ATCAACG 7065 0.0 33.740578 3 CAACGCA 7275 0.0 32.83125 5 AACGCAG 7475 0.0 32.12752 6 TATCAAC 7795 0.0 31.183952 2 ACGCAGA 8735 0.0 27.493214 7 CGCAGAG 8870 0.0 26.809341 8 GTACATG 6690 0.0 26.519104 1 GTCGTAC 180 7.406925E-9 26.143944 1 ATAACGC 255 0.0 25.813362 3 TACATGG 6645 0.0 25.401312 2 GGACCGT 210 1.4333636E-9 24.617325 6 GCAGAGT 10095 0.0 23.416443 9 TATAACG 250 4.3655746E-10 22.568253 2 GTGGTAT 1945 0.0 22.501265 1 ACATGGG 7105 0.0 22.499432 3 TAACGCA 290 1.2187229E-10 21.076672 4 TGGTATC 1930 0.0 20.463442 2 >>END_MODULE