FastQCFastQC Report
Wed 25 May 2016
SRR1033153_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033153_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501607
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA20400.033.4826621
TCAACGC29950.031.525634
TATACCG1351.335502E-831.316515
GTATCAA32700.030.9722581
ATCAACG31750.029.8910873
CAACGCA31650.029.6838955
GTACATG64700.027.8853861
AACGCAG34100.027.6811896
TACATGG64350.026.291682
CGCAGAG38200.024.341368
ACGCAGA39000.024.0828787
ACATGGG68650.023.6793423
TATCAAC41400.022.9306012
GAGTACA45900.021.2441711
GACCGTA1200.00508975419.567347
CTAGTAC6250.019.5446283
GTACCGT1456.639281E-419.4323946
CATGGGG44250.019.3206944
ATACCGC2451.9822073E-719.1680076
TACACCG2553.0098272E-718.4214745
Produced by FastQC (version 0.11.5)