##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1033153_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2501607
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.570472100533777	33.0	31.0	34.0	30.0	34.0
2	31.620322456724818	33.0	31.0	34.0	30.0	34.0
3	31.81120495745335	34.0	31.0	34.0	30.0	34.0
4	35.19798193721076	37.0	35.0	37.0	33.0	37.0
5	35.2683642954309	37.0	35.0	37.0	33.0	37.0
6	35.35401004234478	37.0	36.0	37.0	33.0	37.0
7	35.24580199847538	37.0	35.0	37.0	33.0	37.0
8	35.25708874335577	37.0	35.0	37.0	33.0	37.0
9	36.90896171940677	39.0	38.0	39.0	34.0	39.0
10-11	36.92471819114673	39.0	38.0	39.0	34.0	39.0
12-13	36.89198003523335	39.0	38.0	39.0	34.0	39.0
14-15	38.33323459680118	41.0	39.0	41.0	34.0	41.0
16-17	38.33583152749412	41.0	39.0	41.0	34.0	41.0
18-19	38.31985779540911	41.0	39.0	41.0	34.0	41.0
20-21	38.36274142980892	41.0	39.0	41.0	34.0	41.0
22-23	38.3144664609589	40.5	39.0	41.0	34.0	41.0
24-25	38.306940498647464	40.5	39.0	41.0	34.0	41.0
26-27	38.27319519013179	40.0	39.0	41.0	34.0	41.0
28-29	38.24690888696746	40.0	38.5	41.0	33.5	41.0
30-31	38.24981581839194	40.0	38.5	41.0	34.0	41.0
32-33	38.32655169257201	41.0	39.0	41.0	34.0	41.0
34-35	38.30099312162142	41.0	39.0	41.0	34.0	41.0
36-37	38.22054323480866	41.0	38.5	41.0	33.0	41.0
38-39	38.14536356030344	41.0	38.0	41.0	33.0	41.0
40-41	38.08919966245698	40.0	38.0	41.0	33.0	41.0
42-43	37.97129365244021	40.0	38.0	41.0	33.0	41.0
44-45	37.839005287401264	40.0	38.0	41.0	33.0	41.0
46-47	37.72048827013995	40.0	38.0	41.0	32.5	41.0
48-49	37.60518338811812	40.0	38.0	41.0	32.0	41.0
50-51	37.493317095770834	40.0	38.0	41.0	32.0	41.0
52-53	37.3568518156529	40.0	37.0	41.0	31.5	41.0
54-55	37.21578889090093	40.0	37.0	41.0	31.0	41.0
56-57	37.066596191967804	40.0	37.0	41.0	31.0	41.0
58-59	36.88035510773675	40.0	36.0	41.0	31.0	41.0
60-61	36.67231903332538	39.5	36.0	41.0	31.0	41.0
62-63	36.42028983769234	39.0	35.0	41.0	30.0	41.0
64-65	36.160135864666195	39.0	35.0	41.0	30.0	41.0
66-67	35.88090575378147	38.5	35.0	40.0	30.0	41.0
68-69	35.58306100838381	38.0	35.0	40.0	30.0	41.0
70-71	35.246150174667726	37.0	35.0	40.0	29.0	41.0
72-73	34.88623153037228	37.0	34.0	39.5	29.0	41.0
74-75	34.51576066904194	36.0	34.0	39.0	29.0	41.0
76-77	34.17663945615759	36.0	34.0	39.0	29.0	40.0
78-79	33.79706884414698	35.0	34.0	37.5	28.0	39.5
80-81	33.422674504828294	35.0	34.0	37.0	28.0	39.0
82-83	33.17721248781284	35.0	34.0	37.0	28.0	39.0
84-85	32.884509637205205	35.0	34.0	36.0	28.0	38.0
86-87	32.60570385356293	35.0	34.0	36.0	27.0	37.0
88-89	32.22174406291636	35.0	33.5	36.0	26.5	37.0
90-91	32.01012469184808	35.0	33.0	35.0	26.0	36.5
92-93	31.811946880545186	35.0	33.0	35.0	26.0	36.0
94-95	31.626032985996602	35.0	33.0	35.0	25.0	36.0
96-97	31.434657002478808	35.0	33.0	35.0	25.0	36.0
98-99	31.22879513049012	35.0	33.0	35.0	24.5	35.5
100	31.05616749553387	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.31912511060872717
1101	2	0.225437709815548
1101	3	0.2916023574393982
1101	4	0.237546296260561
1101	5	0.24578550776939068
1101	6	0.2542684323882085
1101	7	0.2583391069634544
1101	8	0.311885056494404
1101	9	0.3169281614696473
1101	10-11	0.31640420128279345
1101	12-13	0.28521777918665947
1101	14-15	0.2930342954924896
1101	16-17	0.30853832189172437
1101	18-19	0.32918114453173786
1101	20-21	0.3112267304656555
1101	22-23	0.3629685298272207
1101	24-25	0.3840472075704966
1101	26-27	0.379027092253466
1101	28-29	0.3965345587290159
1101	30-31	0.41380338707636355
1101	32-33	0.4016631428311044
1101	34-35	0.32323667853031424
1101	36-37	0.4051388305655479
1101	38-39	0.4006452706936372
1101	40-41	0.38977619943998576
1101	42-43	0.34995738319797454
1101	44-45	0.3375754773966264
1101	46-47	0.4375281742551209
1101	48-49	0.3660061186091639
1101	50-51	0.3452260596067802
1101	52-53	0.3699350623333899
1101	54-55	0.29642754845271924
1101	56-57	0.28095569395409825
1101	58-59	0.3472994994970904
1101	60-61	0.2951046081085664
1101	62-63	0.31001115681686287
1101	64-65	0.289600838086173
1101	66-67	0.39262948872053016
1101	68-69	0.3469665622077045
1101	70-71	0.36357946392818974
1101	72-73	0.413881712419375
1101	74-75	0.331083605913868
1101	76-77	0.2567256778086886
1101	78-79	0.27987736800950813
1101	80-81	0.3420298713366847
1101	82-83	0.26894675962613235
1101	84-85	0.22689611986854175
1101	86-87	0.31557225260422683
1101	88-89	0.5052257429498468
1101	90-91	0.5311502597040558
1101	92-93	0.5603392518885819
1101	94-95	0.5331710555051288
1101	96-97	0.4949596154471756
1101	98-99	0.5213843195380718
1101	100	0.5706125084882103
1102	1	0.2186423612171211
1102	2	0.22045425579486988
1102	3	0.2771667958921604
1102	4	0.25591662881574706
1102	5	0.11883480867491869
1102	6	0.1810670538594863
1102	7	0.11933107270509424
1102	8	0.20625405413682785
1102	9	0.2851328690196695
1102	10-11	0.21728924183408083
1102	12-13	0.21848812545443508
1102	14-15	0.3138688472641391
1102	16-17	0.3161380314490714
1102	18-19	0.2984855029888607
1102	20-21	0.2406915840961048
1102	22-23	0.2785518189663563
1102	24-25	0.2992639326395974
1102	26-27	0.3576596905587195
1102	28-29	0.3307243096389172
1102	30-31	0.40644814993935086
1102	32-33	0.3679424287188624
1102	34-35	0.409034354989096
1102	36-37	0.39077666443829173
1102	38-39	0.33410003482876505
1102	40-41	0.4164864738075167
1102	42-43	0.40854382127734823
1102	44-45	0.363861033078841
1102	46-47	0.3603821533029432
1102	48-49	0.39074541411225994
1102	50-51	0.32034257535230637
1102	52-53	0.23081038842052237
1102	54-55	0.3707922671049815
1102	56-57	0.1955833144225423
1102	58-59	0.2795639939567067
1102	60-61	0.29712535708053167
1102	62-63	0.3817588764785711
1102	64-65	0.43271492686778856
1102	66-67	0.3856822012533385
1102	68-69	0.3881609704431881
1102	70-71	0.37449914943067597
1102	72-73	0.4564894264969226
1102	74-75	0.3849084696552936
1102	76-77	0.3781491276660276
1102	78-79	0.2944807023175997
1102	80-81	0.3022086637362733
1102	82-83	0.30251700693193584
1102	84-85	0.27257987440981424
1102	86-87	0.29313064264657385
1102	88-89	0.4233725023995589
1102	90-91	0.42469501480208294
1102	92-93	0.4239880740098023
1102	94-95	0.4883078675418133
1102	96-97	0.6318353000442976
1102	98-99	0.5406163977560325
1102	100	0.4412982042701863
1103	1	0.2345097562188485
1103	2	0.21634756575046765
1103	3	0.24996700250596504
1103	4	0.3345173015137277
1103	5	0.24550014122809927
1103	6	0.2823850590577166
1103	7	0.2797332477645824
1103	8	0.25806382987342147
1103	9	0.32219614570130517
1103	10-11	0.3231205475956429
1103	12-13	0.3230909372790691
1103	14-15	0.31463177837294865
1103	16-17	0.29771956550045076
1103	18-19	0.30317144960053355
1103	20-21	0.3284287418167935
1103	22-23	0.3099276816451706
1103	24-25	0.2660587437134936
1103	26-27	0.37034702956778176
1103	28-29	0.31149037685016623
1103	30-31	0.3510468150505375
1103	32-33	0.3260629889983946
1103	34-35	0.34674442272008577
1103	36-37	0.4071947221321608
1103	38-39	0.3505697459744752
1103	40-41	0.3247453313919735
1103	42-43	0.30656394607176907
1103	44-45	0.36696970464269185
1103	46-47	0.3667985707590802
1103	48-49	0.3352451035097843
1103	50-51	0.31979119079516494
1103	52-53	0.29975715929776214
1103	54-55	0.3569483399347462
1103	56-57	0.30742462763936373
1103	58-59	0.27856733899896113
1103	60-61	0.23649170397436592
1103	62-63	0.32788344391099145
1103	64-65	0.37451584371655144
1103	66-67	0.39301771328221946
1103	68-69	0.35549084390426344
1103	70-71	0.32537783961463873
1103	72-73	0.31048312461538075
1103	74-75	0.32313013916063227
1103	76-77	0.3259301747793515
1103	78-79	0.36523839188436114
1103	80-81	0.3628089105339001
1103	82-83	0.3935032790646247
1103	84-85	0.36059599285341903
1103	86-87	0.2582420244125032
1103	88-89	0.30815161772190436
1103	90-91	0.3375067953182267
1103	92-93	0.3187077116656418
1103	94-95	0.3074338013042812
1103	96-97	0.32122087661993604
1103	98-99	0.4245017238846174
1103	100	0.4730470305377281
1104	1	0.24253013283628988
1104	2	0.18549484733273758
1104	3	0.219940162984944
1104	4	0.1765406492582784
1104	5	0.0960531730076184
1104	6	0.10237007842929557
1104	7	0.1564527679030192
1104	8	0.13231906132705973
1104	9	0.21195466099572968
1104	10-11	0.26019450125475885
1104	12-13	0.2423629725597749
1104	14-15	0.16800258951040803
1104	16-17	0.2348152386017759
1104	18-19	0.2299247332484171
1104	20-21	0.1397090485400696
1104	22-23	0.16303060680267123
1104	24-25	0.15474120145625392
1104	26-27	0.22336573884189903
1104	28-29	0.22796052692958568
1104	30-31	0.26424793831990456
1104	32-33	0.27158017328219586
1104	34-35	0.20273225304271136
1104	36-37	0.2213659521963649
1104	38-39	0.14284226162906322
1104	40-41	0.1998924102080082
1104	42-43	0.23222639677480572
1104	44-45	0.2246187443810257
1104	46-47	0.12924423550440167
1104	48-49	0.07805739552587454
1104	50-51	0.1571194741074251
1104	52-53	0.06264735891448936
1104	54-55	0.09219763669547376
1104	56-57	0.14078855336643414
1104	58-59	0.08688658748619815
1104	60-61	0.15259289358255046
1104	62-63	0.14438745008718
1104	64-65	0.05507168860748379
1104	66-67	0.023336565495732486
1104	68-69	0.016154688818033947
1104	70-71	0.01341859778751342
1104	72-73	0.08047662344237949
1104	74-75	0.09344059524730852
1104	76-77	-0.09215026168961771
1104	78-79	0.018583363443852363
1104	80-81	0.14289627901554525
1104	82-83	0.11523282100890953
1104	84-85	0.0926730165537748
1104	86-87	0.11226515179923524
1104	88-89	0.2022817590765129
1104	90-91	0.10958084696559212
1104	92-93	0.10271179384059437
1104	94-95	0.2124083097003293
1104	96-97	0.16222869577344667
1104	98-99	0.19711555960909521
1104	100	0.26281417188415546
1105	1	0.2995593105551215
1105	2	0.25002897221169107
1105	3	0.2852097832114602
1105	4	0.3467821654049672
1105	5	0.24436534933703769
1105	6	0.31244281220892844
1105	7	0.2592194935145713
1105	8	0.25807166121759195
1105	9	0.39683766592183645
1105	10-11	0.3557470569366288
1105	12-13	0.42229750163223656
1105	14-15	0.45091896671557663
1105	16-17	0.42320401696900234
1105	18-19	0.3755857054180112
1105	20-21	0.4230721174634766
1105	22-23	0.4616318499082226
1105	24-25	0.46202563158550447
1105	26-27	0.462689872562585
1105	28-29	0.5839126447351433
1105	30-31	0.5545521623525786
1105	32-33	0.5263490514346927
1105	34-35	0.5527951451314621
1105	36-37	0.4867909606170713
1105	38-39	0.4875224856196283
1105	40-41	0.471512552517801
1105	42-43	0.5277889520356354
1105	44-45	0.47192609459785473
1105	46-47	0.5319384641684621
1105	48-49	0.48728029666687434
1105	50-51	0.4758382912098682
1105	52-53	0.4874452621606622
1105	54-55	0.4590091656837245
1105	56-57	0.5628464404027795
1105	58-59	0.46463055089827066
1105	60-61	0.4731655799957579
1105	62-63	0.5073596670464724
1105	64-65	0.4933288935672806
1105	66-67	0.5325118370632467
1105	68-69	0.43020962334710333
1105	70-71	0.41555298160292864
1105	72-73	0.37222947069091816
1105	74-75	0.4036196403401462
1105	76-77	0.32504730367605106
1105	78-79	0.42011382531913455
1105	80-81	0.3775403466337508
1105	82-83	0.39605652316882356
1105	84-85	0.36462077664808135
1105	86-87	0.4139261344947016
1105	88-89	0.4313714552998249
1105	90-91	0.5074877772001116
1105	92-93	0.45557325230550205
1105	94-95	0.5553333444357094
1105	96-97	0.5935940856517696
1105	98-99	0.6550732508641985
1105	100	0.7887157128719053
1106	1	0.20936351004081644
1106	2	0.19869798767459912
1106	3	0.19454918708067837
1106	4	0.1632790139908522
1106	5	0.12413282638340206
1106	6	0.1738487883654969
1106	7	0.1285506884084171
1106	8	0.1461928042084395
1106	9	0.126666970417169
1106	10-11	0.10109874923982431
1106	12-13	0.0743007840974812
1106	14-15	0.1042128286293007
1106	16-17	0.16457014486366006
1106	18-19	0.15440465068700604
1106	20-21	0.1762819561974709
1106	22-23	0.1947338119067723
1106	24-25	0.21885477353244198
1106	26-27	0.1746227530102118
1106	28-29	0.23659375121166448
1106	30-31	0.168799888081125
1106	32-33	0.16761693316470172
1106	34-35	0.08643598460114532
1106	36-37	0.1901045926907372
1106	38-39	0.12161591551458883
1106	40-41	0.20444665798415684
1106	42-43	0.1912726370134692
1106	44-45	0.15541402467616905
1106	46-47	0.13670129396172825
1106	48-49	0.15694929272522273
1106	50-51	0.1345581902273807
1106	52-53	0.10233407680598816
1106	54-55	0.0759642502988882
1106	56-57	0.08355649146209743
1106	58-59	0.09435095780921898
1106	60-61	0.07584023937531015
1106	62-63	0.08639077594152411
1106	64-65	0.07434158837225624
1106	66-67	0.16372752050894945
1106	68-69	0.03543503410522675
1106	70-71	0.07927134934702451
1106	72-73	0.1036239011303266
1106	74-75	0.12378483788426564
1106	76-77	0.06536403823057668
1106	78-79	0.08236581245925834
1106	80-81	0.05922973351693628
1106	82-83	-0.00941673171382007
1106	84-85	-0.020829074639820533
1106	86-87	-0.009358333077742031
1106	88-89	0.16485440768495607
1106	90-91	0.12684625097750768
1106	92-93	0.1525024655712528
1106	94-95	0.1382064520686228
1106	96-97	0.13580988046281917
1106	98-99	0.15207760058061126
1106	100	0.16321611558696958
1107	1	0.036933454735549986
1107	2	-0.025813422740654346
1107	3	-0.022086218488990284
1107	4	-0.03908534477537273
1107	5	-0.08043719686602913
1107	6	-0.04955823938636428
1107	7	-0.12409426224682818
1107	8	-0.09850192807851244
1107	9	0.02929476307134138
1107	10-11	-0.021392771675714073
1107	12-13	-0.11551070528592078
1107	14-15	-0.07329275633449583
1107	16-17	-0.16771129045481104
1107	18-19	-0.05210005777167481
1107	20-21	-0.10202660943498643
1107	22-23	-0.08005005929658893
1107	24-25	-0.09753773262445264
1107	26-27	0.0026499343158405964
1107	28-29	0.013259145384004967
1107	30-31	0.006246880883026051
1107	32-33	-0.018879754445286778
1107	34-35	-0.0012890160534766437
1107	36-37	-0.06868726352935539
1107	38-39	-0.05654942559887388
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2216	54-55	-0.44748163237106553
2216	56-57	-0.5225862227209603
2216	58-59	-0.5675496573681897
2216	60-61	-0.4646855091009243
2216	62-63	-0.5354988907115086
2216	64-65	-0.5414871125204925
2216	66-67	-0.613956885759201
2216	68-69	-0.5853228695855108
2216	70-71	-0.5855852535652986
2216	72-73	-0.49374007025515
2216	74-75	-0.5594785821622352
2216	76-77	-0.6399955736384868
2216	78-79	-0.6019293046798282
2216	80-81	-0.5309258101891672
2216	82-83	-0.4977372770883832
2216	84-85	-0.5197914754585184
2216	86-87	-0.5454366629627998
2216	88-89	-0.464207764797802
2216	90-91	-0.5599773868818296
2216	92-93	-0.48759038117511366
2216	94-95	-0.4176572670849481
2216	96-97	-0.4960808305902731
2216	98-99	-0.5945487864776453
2216	100	-0.6150678170640269
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26139.0
3	4619.0
4	2944.0
5	2460.0
6	2098.0
7	2326.0
8	2663.0
9	2914.0
10	3292.0
11	3679.0
12	4002.0
13	4453.0
14	5029.0
15	5261.0
16	5085.0
17	5113.0
18	5143.0
19	5331.0
20	5413.0
21	5597.0
22	6438.0
23	8644.0
24	13314.0
25	18201.0
26	19560.0
27	16987.0
28	17551.0
29	22241.0
30	27525.0
31	33311.0
32	44144.0
33	59242.0
34	82896.0
35	124563.0
36	228548.0
37	491631.0
38	824550.0
39	358028.0
40	672.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.697484070647036	20.201190926435206	13.405803349946838	23.695521652970918
2	14.89001427949388	20.215649172685826	41.43214358895548	23.46219295886481
3	17.815836779363657	25.757066662186627	30.332626927116955	26.094469631332757
4	11.609076305445727	16.78958946597665	40.19085659122274	31.410477637354884
5	12.160006398656282	38.75550134471761	36.45106527629198	12.63342698033413
6	30.30650354565131	39.18358470184786	18.05475710549482	12.455154647006006
7	26.81321501686837	32.9191697379136	23.102173279492067	17.165441965725964
8	24.279923413588612	37.93110341444419	20.551433356508625	17.23753981545857
9	24.593028834620736	17.115658334532778	21.490788274240753	36.80052455660574
10-11	23.217447905733938	27.60115485602083	29.620473625309913	19.560923612935316
12-13	23.829731091227483	24.839318412614645	29.67014097120605	21.660809524951823
14-15	21.03835601760153	26.967158254656066	26.521062744837764	25.473422982904637
16-17	20.858708550701923	30.389828109556966	27.12244235400987	21.629020985731238
18-19	20.750155279472065	29.486027446333875	28.96820264235984	20.795614631834223
20-21	21.92049151224942	28.756733263601674	28.405855860178423	20.916919363970482
22-23	21.567454681280175	28.45052667687909	28.541403001717374	21.440615640123355
24-25	21.425619739707024	28.668133152898083	28.44434172954345	21.461905377851444
26-27	21.159762338899963	28.57844541525222	28.567830189980548	21.69396205586727
28-29	21.168684015036355	28.584448359106933	28.660853579450634	21.586014046406078
30-31	21.404884793105776	29.04977352046632	28.292169593568296	21.253172092859607
32-33	21.07138201850944	28.707545879758207	28.775418893447963	21.445653208284384
34-35	21.232556596109436	28.551629756855824	28.790911838645915	21.424901808388828
36-37	21.554971339568347	28.809970424065646	28.286174949767286	21.34888328659872
38-39	21.292740702241858	28.919131243066037	28.455787548350344	21.332340506341765
40-41	21.080049317210893	28.726915539478952	28.703948015438012	21.48908712787214
42-43	21.11358395645305	28.53381237327056	28.71848954502133	21.634114125255056
44-45	21.24534656125269	28.716514430457472	28.62096701975233	21.417171988537508
46-47	21.28590286567293	28.892277210940104	28.529680555144676	21.292139368242292
48-49	21.246020375040175	28.688838501182495	28.608726312136916	21.45641481164041
50-51	21.256906557736844	28.60962962341096	28.56369584241315	21.56976797643905
52-53	21.23028120027824	28.619084360083473	28.641211791702315	21.50942264793597
54-55	21.374193040308707	29.02540416442969	28.367903661735127	21.23249913352647
56-57	21.22372014634209	28.676101403297814	28.467649476161842	21.632528974198255
58-59	21.039613212172647	28.40755434731553	28.830396160104637	21.722436280407184
60-61	21.34723772951628	28.787300955302612	28.424380304543934	21.44108101063717
62-63	21.19421457373836	28.767908561450668	28.501671179243722	21.536205685567257
64-65	21.185357789385254	28.546018596081584	28.576199901780836	21.69242371275232
66-67	21.060832798499014	28.565129628448883	28.513181908761897	21.86085566429021
68-69	21.44674461773641	28.688542775781105	28.46445960096765	21.400253005514834
70-71	21.375579965769205	28.470825170889153	28.50540375291544	21.6481911104262
72-73	21.14128897696735	28.586250765772924	28.57163986836201	21.700820388897718
74-75	21.235555191336562	28.616726246453446	28.455526029387805	21.692192532822183
76-77	21.237760353579567	28.61101050488866	28.37335328934376	21.777875852188007
78-79	21.30704662270002	28.68648730220756	28.47013862585411	21.536327449238314
80-81	21.26485349877974	28.51434030977228	28.52109621569329	21.69970997575469
82-83	21.10896310438673	28.5565234910919	28.689481376301995	21.645032028219372
84-85	21.108748780497066	28.488987646167956	28.537597867546342	21.86466570578863
86-87	21.247372126128354	28.601201576464486	28.43810250217166	21.71332379523551
88-89	21.047197186358318	28.502881143505416	28.526005092496803	21.923916577639464
90-91	21.10316013687545	28.440990561256914	28.54429468441264	21.911554617454996
92-93	21.202676036427203	28.570222026404434	28.434867783779893	21.79223415338847
94-95	20.977890104985057	28.29640308310035	28.629081956302976	22.09662485561162
96-97	21.07087185370472	28.293812191286634	28.62688635970201	22.00842959530664
98-99	21.06525671485329	28.391143408433052	28.50811665236167	22.03548322435199
100	21.155685054289474	28.35293105264724	28.365275967145635	22.126107925917644
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	398.0
1	350.0
2	308.5
3	266.0
4	201.0
5	169.5
6	157.5
7	165.5
8	160.0
9	157.5
10	161.0
11	177.5
12	200.5
13	228.0
14	264.5
15	316.0
16	414.5
17	574.0
18	865.5
19	1316.5
20	1948.0
21	2895.5
22	4355.0
23	6754.0
24	9870.0
25	13378.5
26	18367.0
27	24998.5
28	32869.5
29	40954.5
30	48583.5
31	56571.0
32	64060.5
33	71052.0
34	77657.0
35	82788.0
36	87344.5
37	91606.0
38	93720.0
39	93681.5
40	94149.5
41	94373.0
42	92242.5
43	90041.0
44	88229.5
45	85850.0
46	84135.5
47	81721.5
48	78513.5
49	75972.5
50	72952.5
51	69292.0
52	64502.5
53	60115.5
54	56928.5
55	53504.5
56	49012.0
57	44431.0
58	40276.0
59	35444.5
60	30758.5
61	26267.5
62	22121.5
63	18398.0
64	15045.5
65	12145.5
66	9416.5
67	6966.5
68	5085.0
69	3830.0
70	2770.5
71	1915.0
72	1422.0
73	1056.0
74	800.0
75	574.5
76	365.0
77	264.0
78	200.5
79	114.5
80	69.5
81	46.5
82	36.5
83	33.0
84	26.0
85	15.5
86	10.0
87	11.0
88	10.5
89	6.5
90	5.0
91	3.5
92	2.0
93	1.5
94	3.0
95	3.0
96	1.0
97	1.0
98	3.0
99	3.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.32994790948378383
2	0.11668499488528773
3	0.06511814205828494
4	0.03941466425381765
5	0.04325219748745507
6	0.00931401295247415
7	0.007195374813070159
8	0.015190235716481445
9	0.017868514119124226
10-11	0.02062674113080112
12-13	0.015470055848100843
14-15	0.01842815438236302
16-17	0.019147691863670033
18-19	0.01884788457979211
20-21	0.02362481396958035
22-23	0.016209580481666385
24-25	0.02468413303928235
26-27	0.01928760192947973
28-29	0.018747948818499467
30-31	0.019907203649494106
32-33	0.024504248668955595
34-35	0.014990364193896162
36-37	0.009953601824747053
38-39	0.013271469099662737
40-41	0.009953601824747053
42-43	0.008594475471167134
44-45	0.009234064343440037
46-47	0.00819473242599657
48-49	0.00459704501946149
50-51	0.007195374813070159
52-53	0.007475194944689554
54-55	0.003517738797500966
56-57	0.002838175620711007
58-59	0.003217931513623043
60-61	0.0038375332336374177
62-63	0.0031979443613645146
64-65	0.002338496814247802
66-67	0.002138625291662519
68-69	0.003217931513623043
70-71	0.002338496814247802
72-73	0.0019787280735942933
74-75	0.002378471118764858
76-77	0.004197301974290926
78-79	0.0035777002542765513
80-81	0.003677636015569192
82-83	0.0025183811845745554
84-85	0.003018059991037761
86-87	0.002398458271023386
88-89	0.5999143750397244
90-91	0.6099679126257642
92-93	0.6063502380669705
94-95	0.5925990773131031
96-97	0.5905603877827332
98-99	0.5881619295117099
100	0.5897409145401336
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2501607.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.07911114699708
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.24431308052957	54.741267110573354
2	13.156673546228914	18.17702628449406
3	3.8268352885323305	7.930631407133265
4	1.514938981972585	4.186025532664111
5	0.7353372415033655	2.539822151816861
6	0.3969522545276713	1.645266534634085
7	0.25866809404811464	1.2507993413252123
8	0.17776393520774086	0.9823819710514494
9	0.11983536536561064	0.7450308471088819
>10	0.5436394295823553	6.32861511359052
>50	0.019751614320192845	0.9063925073459547
>100	0.0052333068660162935	0.5438383410171322
>500	5.786131550996338E-5	0.02290285724513481
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.9974304517056435E-5	0.0
10-11	0.0	0.0	0.0	3.9974304517056435E-5	0.0
12-13	0.0	0.0	0.0	3.9974304517056435E-5	0.0
14-15	1.9987152258528217E-5	0.0	0.0	3.9974304517056435E-5	0.0
16-17	3.9974304517056435E-5	0.0	0.0	5.996145677558465E-5	0.0
18-19	3.9974304517056435E-5	0.0	0.0	7.994860903411287E-5	0.0
20-21	3.9974304517056435E-5	0.0	0.0	1.5989721806822574E-4	0.0
22-23	9.99357612926411E-5	0.0	0.0	2.398458271023386E-4	0.0
24-25	1.5989721806822574E-4	0.0	0.0	3.997430451705644E-4	0.0
26-27	1.5989721806822574E-4	0.0	0.0	5.196659587217336E-4	0.0
28-29	1.7988437032675396E-4	0.0	0.0	0.0011192805264775801	0.0
30-31	2.198586748438104E-4	0.0	0.0	0.003757584624603305	0.0
32-33	2.598329793608668E-4	0.0	0.0	0.010892997980897878	0.0
34-35	2.7982013161939503E-4	0.0	0.0	0.021246342850815496	0.0
36-37	2.7982013161939503E-4	0.0	0.0	0.039514600015110285	0.0
38-39	2.7982013161939503E-4	0.0	0.0	0.07077450614744842	0.0
40-41	2.7982013161939503E-4	0.0	0.0	0.10881005689542762	0.0
42-43	2.7982013161939503E-4	0.0	0.0	0.1464058902937192	0.0
44-45	2.7982013161939503E-4	0.0	0.0	0.18781926977338964	0.0
46-47	2.7982013161939503E-4	0.0	0.0	0.2409651076288162	0.0
48-49	2.7982013161939503E-4	0.0	0.0	0.3026254723463757	0.0
50-51	2.7982013161939503E-4	0.0	0.0	0.3715611604860396	0.0
52-53	2.7982013161939503E-4	0.0	0.0	0.43961741392632814	0.0
54-55	2.7982013161939503E-4	0.0	0.0	0.5129103012583511	0.0
56-57	2.7982013161939503E-4	0.0	0.0	0.598495287229369	0.0
58-59	3.197944361364515E-4	0.0	0.0	0.7018488515582184	0.0
60-61	3.5976874065350793E-4	0.0	0.0	0.808540270314242	0.0
62-63	3.7975589291203615E-4	0.0	0.0	0.9174902372754794	0.0
64-65	3.997430451705644E-4	0.0	0.0	1.0299979173387346	0.0
66-67	3.997430451705644E-4	0.0	0.0	1.1558570151106868	0.0
68-69	3.997430451705644E-4	0.0	0.0	1.3048812223502733	0.0
70-71	3.997430451705644E-4	0.0	0.0	1.4709144961618672	0.0
72-73	3.997430451705644E-4	0.0	0.0	1.6458220655762474	0.0
74-75	3.997430451705644E-4	0.0	0.0	1.8178914593699171	0.0
76-77	3.997430451705644E-4	0.0	0.0	2.0009337997535184	0.0
78-79	3.997430451705644E-4	0.0	0.0	2.2087002474809196	0.0
80-81	4.397173496876208E-4	0.0	0.0	2.4393519845443348	0.0
82-83	4.397173496876208E-4	0.0	0.0	2.6798374005189465	0.0
84-85	4.397173496876208E-4	3.9974304517056435E-5	0.0	2.920282842189041	0.0
86-87	4.397173496876208E-4	3.9974304517056435E-5	0.0	3.1762583011640118	0.0
88	4.397173496876208E-4	3.9974304517056435E-5	0.0	3.3840247488914126	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2040	0.0	33.482662	1
TCAACGC	2995	0.0	31.52563	4
TATACCG	135	1.335502E-8	31.31651	5
GTATCAA	3270	0.0	30.972258	1
ATCAACG	3175	0.0	29.891087	3
CAACGCA	3165	0.0	29.683895	5
GTACATG	6470	0.0	27.885386	1
AACGCAG	3410	0.0	27.681189	6
TACATGG	6435	0.0	26.29168	2
CGCAGAG	3820	0.0	24.34136	8
ACGCAGA	3900	0.0	24.082878	7
ACATGGG	6865	0.0	23.679342	3
TATCAAC	4140	0.0	22.930601	2
GAGTACA	4590	0.0	21.244171	1
GACCGTA	120	0.005089754	19.56734	7
CTAGTAC	625	0.0	19.544628	3
GTACCGT	145	6.639281E-4	19.432394	6
CATGGGG	4425	0.0	19.320694	4
ATACCGC	245	1.9822073E-7	19.168007	6
TACACCG	255	3.0098272E-7	18.421474	5
>>END_MODULE