FastQCFastQC Report
Wed 25 May 2016
SRR1033153_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1033153_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2501607
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44220.17676637457442354No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36110.1443472136110908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35900.047.6972161
GTATCAA64850.040.4771841
ATCAACG74550.034.872013
TCAACGC74700.034.8019834
CAACGCA76800.033.8510445
AACGCAG79250.032.8526956
TATCAAC82850.031.4358632
ACGCAGA90350.028.712537
CGCAGAG90800.028.310888
GTACATG65450.025.9467931
TACATGG66150.024.945112
GCAGAGT102650.024.8132429
TAGCGCG950.001303236624.742634
CGCGCGT950.001305668124.7347167
ACATGGG66400.023.8594953
GTGGTAT20650.021.1860031
GAGTACT68350.021.00425112-13
AGAGTAC92350.020.55832110-11
CATGGGG40500.020.5455474
TGGTATC19800.020.4193272
Produced by FastQC (version 0.11.5)